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SH2-domain substitutions increase fitness in <t>BTK.</t> Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by <t>their</t> <t>sequence</t> relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .
Btk Sequence, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/btk sequence/product/New England Biolabs
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btk sequence - by Bioz Stars, 2026-02
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direct sequencing of exonic coding region of the btk gene  (Guangzhou Kingmed Diagnostics Group Co Ltd)
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SH2-domain substitutions increase fitness in <t>BTK.</t> Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by <t>their</t> <t>sequence</t> relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .
Direct Sequencing Of Exonic Coding Region Of The Btk Gene, supplied by Guangzhou Kingmed Diagnostics Group Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/direct sequencing of exonic coding region of the btk gene/product/Guangzhou Kingmed Diagnostics Group Co Ltd
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direct sequencing of exonic coding region of the btk gene - by Bioz Stars, 2026-02
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btk genomic sequencing  (GeneDx Inc)
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SH2-domain substitutions increase fitness in <t>BTK.</t> Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by <t>their</t> <t>sequence</t> relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .
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btk dna sequencing test  (LabCorp)
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SH2-domain substitutions increase fitness in <t>BTK.</t> Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by <t>their</t> <t>sequence</t> relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .
Btk Dna Sequencing Test, supplied by LabCorp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/btk dna sequencing test/product/LabCorp
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protein sequence of btk  (Biotechnology Information)
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Biotechnology Information protein sequence of btk
SH2-domain substitutions increase fitness in <t>BTK.</t> Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by <t>their</t> <t>sequence</t> relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .
Protein Sequence Of Btk, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SH2-domain substitutions increase fitness in BTK. Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by their sequence relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: BTK autoinhibition analyzed by high-throughput swaps of SH2 domains

doi: 10.1073/pnas.2502688122

Figure Lengend Snippet: SH2-domain substitutions increase fitness in BTK. Fitness scores for the 188 SH2-domain chimeras corresponding to Tec kinases or ancestral SH2 domains are shown. SH2 domains are ordered by their sequence relationships. The fitness bars, measured using the Jurkat assay, are colored by the human Tec kinase to which they are most closely related, requiring at least 75% sequence identity to display that color (sequences without 75% sequence identity to a human Tec kinase are colored purple). Error bars represent SEM and points are the individual replicate values. Sequence numbering corresponds to SI Appendix , Table S1 .

Article Snippet: The assembled BTK sequence was digested with BamHI HF (New England Biolabs) and XmaI (New England Biolabs) in a 30 μL reaction containing 3 μL of CutSmart buffer (New England Biolabs).

Techniques: Sequencing

Screening αI kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human BTK SH2 genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: BTK autoinhibition analyzed by high-throughput swaps of SH2 domains

doi: 10.1073/pnas.2502688122

Figure Lengend Snippet: Screening αI kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human BTK SH2 genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.

Article Snippet: The assembled BTK sequence was digested with BamHI HF (New England Biolabs) and XmaI (New England Biolabs) in a 30 μL reaction containing 3 μL of CutSmart buffer (New England Biolabs).

Techniques: Sequencing