human breast cancer cell line bt 474  (ATCC)


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    ATCC human breast cancer cell line bt 474
    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.
    Human Breast Cancer Cell Line Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody"

    Article Title: Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody

    Journal: mAbs

    doi: 10.1080/19420862.2018.1486946

    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.
    Figure Legend Snippet: ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.

    Techniques Used: Activity Assay

    human breast ductal carcinoma cell line bt 474  (ATCC)


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    ATCC human breast ductal carcinoma cell line bt 474
    Human Breast Ductal Carcinoma Cell Line Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast ductal carcinoma cell line bt 474  (ATCC)


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    ATCC human breast ductal carcinoma cell line bt 474
    Cellular binding/uptake specificity and distribution of mAb-IR700 and talaporfin sodium. Fluorescence images of live cells stained for nuclei (blue) after incubation with mAb-IR700 or talaporfin sodium (magenta). ( A ) A431 cells, ( B ) BALB/3T3 cells, and ( C ) <t>BT-474</t> cells. Scale bar = 20 µm.
    Human Breast Ductal Carcinoma Cell Line Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy"

    Article Title: In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy

    Journal: Cancers

    doi: 10.3390/cancers15133400

    Cellular binding/uptake specificity and distribution of mAb-IR700 and talaporfin sodium. Fluorescence images of live cells stained for nuclei (blue) after incubation with mAb-IR700 or talaporfin sodium (magenta). ( A ) A431 cells, ( B ) BALB/3T3 cells, and ( C ) BT-474 cells. Scale bar = 20 µm.
    Figure Legend Snippet: Cellular binding/uptake specificity and distribution of mAb-IR700 and talaporfin sodium. Fluorescence images of live cells stained for nuclei (blue) after incubation with mAb-IR700 or talaporfin sodium (magenta). ( A ) A431 cells, ( B ) BALB/3T3 cells, and ( C ) BT-474 cells. Scale bar = 20 µm.

    Techniques Used: Binding Assay, Fluorescence, Staining, Incubation

    Evaluation of cell death induced by NIR-PIT and TS-PDT. Hoechst and PI double-stained images 1 day after irradiation. ( A ) A431 cells, ( B ) BALB/3T3 cells, and ( C ) BT-474 cells. Scale bar = 50 µm. ( D ) LDH assay at 1 day after irradiation in the three cell lines. Data are presented as means ± SD (n = 3; Tukey’s test; * p < 0.05).
    Figure Legend Snippet: Evaluation of cell death induced by NIR-PIT and TS-PDT. Hoechst and PI double-stained images 1 day after irradiation. ( A ) A431 cells, ( B ) BALB/3T3 cells, and ( C ) BT-474 cells. Scale bar = 50 µm. ( D ) LDH assay at 1 day after irradiation in the three cell lines. Data are presented as means ± SD (n = 3; Tukey’s test; * p < 0.05).

    Techniques Used: Staining, Irradiation, Lactate Dehydrogenase Assay

    Analysis of ICD markers following NIR-PIT or TS-PDT. ( A ) Confocal microscopy images of HMGB1 and quantification of HMGB1 translocation. HMGB1 was stained at 1 h after treatment of A431 and BT-474 cells with NIR-PIT or TS-PDT. Scale bar = 20 µm. Fluorescence intensity of nuclear HMGB1 was quantified; data are presented as means ± SD from five microscopic fields (Tukey’s test; * p < 0.05). ( B ) ATP assays of culture supernatants. Data are presented as means ± SD (n = 3; Steel–Dwass test).
    Figure Legend Snippet: Analysis of ICD markers following NIR-PIT or TS-PDT. ( A ) Confocal microscopy images of HMGB1 and quantification of HMGB1 translocation. HMGB1 was stained at 1 h after treatment of A431 and BT-474 cells with NIR-PIT or TS-PDT. Scale bar = 20 µm. Fluorescence intensity of nuclear HMGB1 was quantified; data are presented as means ± SD from five microscopic fields (Tukey’s test; * p < 0.05). ( B ) ATP assays of culture supernatants. Data are presented as means ± SD (n = 3; Steel–Dwass test).

    Techniques Used: Confocal Microscopy, Translocation Assay, Staining, Fluorescence

    human breast ductal carcinoma cell line bt 474  (ATCC)


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    ATCC human breast ductal carcinoma cell line bt 474
    Human Breast Ductal Carcinoma Cell Line Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell line bt 474  (ATCC)


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    ATCC human breast cancer cell line bt 474
    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.
    Human Breast Cancer Cell Line Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell line bt 474 - by Bioz Stars, 2024-07
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    1) Product Images from "Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody"

    Article Title: Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody

    Journal: mAbs

    doi: 10.1080/19420862.2018.1486946

    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.
    Figure Legend Snippet: ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.

    Techniques Used: Activity Assay

    human bt474 breast cancer cell line  (ATCC)


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    ATCC human bt474 breast cancer cell line
    Effect of heregulin α1 (HRG α1) and p38 MAPK inhibitor (SB 203580) on Hsp27 phosphorylation . Cultures of cells from the <t>BT474</t> breast cancer cell line were treated with HRG α1 for 10 and 30 min (A) or SB 203580 for 10 hours (B) and total cell lysates were extracted using M-PER reagent (Pierce). Equal amounts of proteins (20 μg) were separated by SDS-PAGE and transferred onto PVDF membrane. The membranes were blocked for 1 hour, followed by being probed with the respective primary antibodies (anti-Hsp27, anti-pSer 15 , anti-pSer 78 and anti-pSer 82 ), and HRP-conjugated secondary antibody. The signals were captured and their intensities were detected as described in Figure 2. The phosphorylation levels of pSer 15 , pSer 78 and pSer 82 were expressed as the ratios of intensity probed with phosphorylation site-specific antibody to the intensity probed with anti-Hsp27. Data with ± SD represents the average of triplicate experiments. C: control; SB: inhibitor SB203580. For the control of HRG-treated cells, untreated cells were cultured for 10 and 30 min and equal amounts of cellular proteins from both time intervals were mixed and used as control. For the control of inhibitor-treated cells, cells were treated with DMSO for 10 hours and cellular proteins were used as the control. * p < 0.05 (student t -test)
    Human Bt474 Breast Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Phosphorylation of Ser 78 of Hsp27 correlated with HER-2/ neu status and lymph node positivity in breast cancer"

    Article Title: Phosphorylation of Ser 78 of Hsp27 correlated with HER-2/ neu status and lymph node positivity in breast cancer

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-6-52

    Effect of heregulin α1 (HRG α1) and p38 MAPK inhibitor (SB 203580) on Hsp27 phosphorylation . Cultures of cells from the BT474 breast cancer cell line were treated with HRG α1 for 10 and 30 min (A) or SB 203580 for 10 hours (B) and total cell lysates were extracted using M-PER reagent (Pierce). Equal amounts of proteins (20 μg) were separated by SDS-PAGE and transferred onto PVDF membrane. The membranes were blocked for 1 hour, followed by being probed with the respective primary antibodies (anti-Hsp27, anti-pSer 15 , anti-pSer 78 and anti-pSer 82 ), and HRP-conjugated secondary antibody. The signals were captured and their intensities were detected as described in Figure 2. The phosphorylation levels of pSer 15 , pSer 78 and pSer 82 were expressed as the ratios of intensity probed with phosphorylation site-specific antibody to the intensity probed with anti-Hsp27. Data with ± SD represents the average of triplicate experiments. C: control; SB: inhibitor SB203580. For the control of HRG-treated cells, untreated cells were cultured for 10 and 30 min and equal amounts of cellular proteins from both time intervals were mixed and used as control. For the control of inhibitor-treated cells, cells were treated with DMSO for 10 hours and cellular proteins were used as the control. * p < 0.05 (student t -test)
    Figure Legend Snippet: Effect of heregulin α1 (HRG α1) and p38 MAPK inhibitor (SB 203580) on Hsp27 phosphorylation . Cultures of cells from the BT474 breast cancer cell line were treated with HRG α1 for 10 and 30 min (A) or SB 203580 for 10 hours (B) and total cell lysates were extracted using M-PER reagent (Pierce). Equal amounts of proteins (20 μg) were separated by SDS-PAGE and transferred onto PVDF membrane. The membranes were blocked for 1 hour, followed by being probed with the respective primary antibodies (anti-Hsp27, anti-pSer 15 , anti-pSer 78 and anti-pSer 82 ), and HRP-conjugated secondary antibody. The signals were captured and their intensities were detected as described in Figure 2. The phosphorylation levels of pSer 15 , pSer 78 and pSer 82 were expressed as the ratios of intensity probed with phosphorylation site-specific antibody to the intensity probed with anti-Hsp27. Data with ± SD represents the average of triplicate experiments. C: control; SB: inhibitor SB203580. For the control of HRG-treated cells, untreated cells were cultured for 10 and 30 min and equal amounts of cellular proteins from both time intervals were mixed and used as control. For the control of inhibitor-treated cells, cells were treated with DMSO for 10 hours and cellular proteins were used as the control. * p < 0.05 (student t -test)

    Techniques Used: SDS Page, Cell Culture

    breast cell lines bt 474  (ATCC)


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    ATCC breast cell lines bt 474
    Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.
    Breast Cell Lines Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers"

    Article Title: CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-11-418

    Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.
    Figure Legend Snippet: Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.

    Techniques Used: Flow Cytometry, Expressing

    breast carcinoma cell lines bt 474  (ATCC)


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    ATCC breast carcinoma cell lines bt 474
    Breast Carcinoma Cell Lines Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell line bt474  (ATCC)


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    ATCC human breast cancer cell line bt474
    Phosphorylated levels of β1 downstream kinases are increased upon acquisition of resistance to lapatinib (L) . ( A ) Parental (P) <t>BT474</t> and ( B ) HCC1954 cells resistant to lapatinib (LRes), trastuzumab (TRes), and combination (LTRes) treatment strategies were developed by long-term exposure in 2D. Protein extracts were probed for β1, pHER2, pFAK, and pSrc, as well as totals.
    Human Breast Cancer Cell Line Bt474, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib"

    Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

    Journal: Breast Cancer Research : BCR

    doi: 10.1186/bcr2936

    Phosphorylated levels of β1 downstream kinases are increased upon acquisition of resistance to lapatinib (L) . ( A ) Parental (P) BT474 and ( B ) HCC1954 cells resistant to lapatinib (LRes), trastuzumab (TRes), and combination (LTRes) treatment strategies were developed by long-term exposure in 2D. Protein extracts were probed for β1, pHER2, pFAK, and pSrc, as well as totals.
    Figure Legend Snippet: Phosphorylated levels of β1 downstream kinases are increased upon acquisition of resistance to lapatinib (L) . ( A ) Parental (P) BT474 and ( B ) HCC1954 cells resistant to lapatinib (LRes), trastuzumab (TRes), and combination (LTRes) treatment strategies were developed by long-term exposure in 2D. Protein extracts were probed for β1, pHER2, pFAK, and pSrc, as well as totals.

    Techniques Used:

    β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor PF 573228 was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.
    Figure Legend Snippet: β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor PF 573228 was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.

    Techniques Used: Staining, TUNEL Assay, Labeling, Western Blot

    Percent growth inhibition of cells in response to HER-targeted therapies
    Figure Legend Snippet: Percent growth inhibition of cells in response to HER-targeted therapies

    Techniques Used: Inhibition

    HER2 and the β1 pathway play alternate roles in resistance to lapatinib-containing regimens, in comparison to trastuzumab . ( A ) Parental, LRes, and TRes cells were plated on lrECM in the presence of lapatinib and/or trastuzumab and assayed for response. ( B ) 3D cultures of parental, LRes, and TRes BT474 cells were harvested for protein and probed for phosphorylated and total HER receptors.
    Figure Legend Snippet: HER2 and the β1 pathway play alternate roles in resistance to lapatinib-containing regimens, in comparison to trastuzumab . ( A ) Parental, LRes, and TRes cells were plated on lrECM in the presence of lapatinib and/or trastuzumab and assayed for response. ( B ) 3D cultures of parental, LRes, and TRes BT474 cells were harvested for protein and probed for phosphorylated and total HER receptors.

    Techniques Used:

    β1 inhibition impedes colony grow of BT474 LRes and LTRes cells, but not parental or TRes . ( A ) Parental, LRes, LTRes, and TRes cells were plated in lrECM, subjected to HER2 and/or β1 inhibitors on Day 0, and propagated for 10 to 12 days. ( B ) The Hs_ITGB1_5 siRNA was validated both in 2D and 3D (top), transfected at 40 nMsi into parental, LRes, LTRes, and TRes cells, which were then grown on lrECM for 10 days (bottom).
    Figure Legend Snippet: β1 inhibition impedes colony grow of BT474 LRes and LTRes cells, but not parental or TRes . ( A ) Parental, LRes, LTRes, and TRes cells were plated in lrECM, subjected to HER2 and/or β1 inhibitors on Day 0, and propagated for 10 to 12 days. ( B ) The Hs_ITGB1_5 siRNA was validated both in 2D and 3D (top), transfected at 40 nMsi into parental, LRes, LTRes, and TRes cells, which were then grown on lrECM for 10 days (bottom).

    Techniques Used: Inhibition, Transfection

    human breast cancer cell line bt  (ATCC)


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    ATCC human breast cancer cell line bt
    A. Effects of FS-93 on cell proliferation. <t>BT-474,</t> NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 72 h. Cell viability was assessed with SRB assay. Bars represent means±SD. B. Effects of FS-93 on degradation of client onco-proteins. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell lysates were prepared and analyzed with immunoblotting.
    Human Breast Cancer Cell Line Bt, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "FS-93, an Hsp90 inhibitor, induces G2/M arrest and apoptosis via the degradation of client proteins in oncogene addicted and derived resistant cancer cells"

    Article Title: FS-93, an Hsp90 inhibitor, induces G2/M arrest and apoptosis via the degradation of client proteins in oncogene addicted and derived resistant cancer cells

    Journal: Oncoscience

    doi:

    A. Effects of FS-93 on cell proliferation. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 72 h. Cell viability was assessed with SRB assay. Bars represent means±SD. B. Effects of FS-93 on degradation of client onco-proteins. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell lysates were prepared and analyzed with immunoblotting.
    Figure Legend Snippet: A. Effects of FS-93 on cell proliferation. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 72 h. Cell viability was assessed with SRB assay. Bars represent means±SD. B. Effects of FS-93 on degradation of client onco-proteins. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell lysates were prepared and analyzed with immunoblotting.

    Techniques Used: Sulforhodamine B Assay, Western Blot

    A ., B. Effects of FS-93 on cell cycle distribution. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell cycle distribution was analyzed by FACS after propidium iodide staining. Representative images A. and quantification results B. were presented. Bars represent means±SD. C. Impacts of FS-93 on G2/M transition regulators. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h and cells lysates were immunoblotted with the indicated antibodies.
    Figure Legend Snippet: A ., B. Effects of FS-93 on cell cycle distribution. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell cycle distribution was analyzed by FACS after propidium iodide staining. Representative images A. and quantification results B. were presented. Bars represent means±SD. C. Impacts of FS-93 on G2/M transition regulators. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h and cells lysates were immunoblotted with the indicated antibodies.

    Techniques Used: Staining

    A. Effects of FS-93 on apoptosis induction. BT-474, NCI-H3122 and EBC-1 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h. Apoptosis was determined with annexin-V/PI assay. Bars represent means±SD. B. Impacts of FS-93 on apoptotic proteins. BT-474 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h and analyzed by immunoblotted with the indicated antibodies.
    Figure Legend Snippet: A. Effects of FS-93 on apoptosis induction. BT-474, NCI-H3122 and EBC-1 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h. Apoptosis was determined with annexin-V/PI assay. Bars represent means±SD. B. Impacts of FS-93 on apoptotic proteins. BT-474 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h and analyzed by immunoblotted with the indicated antibodies.

    Techniques Used:

    human breast ductal carcinoma cell line bt 474  (ATCC)


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    ATCC human breast ductal carcinoma cell line bt 474
    Protoporphyrin IX induction capacity (fluorescence intensity) of ALA-Hex (orange shades) and PSI-ALA-Hex (blue shades) at concentrations of 0.033 ( • / • ), 0.1 ( • / • ), 0.33 ( • / • ), and 1 mM ( • / • ) in <t>BT-474,</t> SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro.
    Human Breast Ductal Carcinoma Cell Line Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enlarging the Scope of 5-Aminolevulinic Acid-Mediated Photodiagnosis towards Breast Cancers"

    Article Title: Enlarging the Scope of 5-Aminolevulinic Acid-Mediated Photodiagnosis towards Breast Cancers

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232314900

    Protoporphyrin IX induction capacity (fluorescence intensity) of ALA-Hex (orange shades) and PSI-ALA-Hex (blue shades) at concentrations of 0.033 ( • / • ), 0.1 ( • / • ), 0.33 ( • / • ), and 1 mM ( • / • ) in BT-474, SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro.
    Figure Legend Snippet: Protoporphyrin IX induction capacity (fluorescence intensity) of ALA-Hex (orange shades) and PSI-ALA-Hex (blue shades) at concentrations of 0.033 ( • / • ), 0.1 ( • / • ), 0.33 ( • / • ), and 1 mM ( • / • ) in BT-474, SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro.

    Techniques Used: Fluorescence, In Vitro

    Evaluation of the dark toxicity of ALA-Hex ( ) and PSI-ALA-Hex ( ) at concentrations of 0.033 mM, 0.1 mM, 0.33 mM, and 1 mM after 30 h of incubation by WST-1 viability measurement in BT-474, SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro.
    Figure Legend Snippet: Evaluation of the dark toxicity of ALA-Hex ( ) and PSI-ALA-Hex ( ) at concentrations of 0.033 mM, 0.1 mM, 0.33 mM, and 1 mM after 30 h of incubation by WST-1 viability measurement in BT-474, SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro.

    Techniques Used: Incubation, In Vitro

    Evaluation of the photodynamic therapy potential of ALA-Hex ( ) and PSI-ALA-Hex ( ) at concentrations of 0.033 mM, 0.1 mM, 0.33 mM, and 1 mM in BT-474, SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro. Cells were incubated for 6 h then exposed to blue light (5 J/cm 2 ), and the PDT efficacy was assessed 24 h post-irradiation by WST-1 viability measurement. Values are normalized against the untreated control exposed to the same blue light fluence.
    Figure Legend Snippet: Evaluation of the photodynamic therapy potential of ALA-Hex ( ) and PSI-ALA-Hex ( ) at concentrations of 0.033 mM, 0.1 mM, 0.33 mM, and 1 mM in BT-474, SK-BR-3, MCF-7, and MDA-MB-231 breast cancer cells in vitro. Cells were incubated for 6 h then exposed to blue light (5 J/cm 2 ), and the PDT efficacy was assessed 24 h post-irradiation by WST-1 viability measurement. Values are normalized against the untreated control exposed to the same blue light fluence.

    Techniques Used: In Vitro, Incubation, Irradiation

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    ATCC human breast cancer cell line bt 474
    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.
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    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.
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    ATCC human bt474 breast cancer cell line
    Effect of heregulin α1 (HRG α1) and p38 MAPK inhibitor (SB 203580) on Hsp27 phosphorylation . Cultures of cells from the <t>BT474</t> breast cancer cell line were treated with HRG α1 for 10 and 30 min (A) or SB 203580 for 10 hours (B) and total cell lysates were extracted using M-PER reagent (Pierce). Equal amounts of proteins (20 μg) were separated by SDS-PAGE and transferred onto PVDF membrane. The membranes were blocked for 1 hour, followed by being probed with the respective primary antibodies (anti-Hsp27, anti-pSer 15 , anti-pSer 78 and anti-pSer 82 ), and HRP-conjugated secondary antibody. The signals were captured and their intensities were detected as described in Figure 2. The phosphorylation levels of pSer 15 , pSer 78 and pSer 82 were expressed as the ratios of intensity probed with phosphorylation site-specific antibody to the intensity probed with anti-Hsp27. Data with ± SD represents the average of triplicate experiments. C: control; SB: inhibitor SB203580. For the control of HRG-treated cells, untreated cells were cultured for 10 and 30 min and equal amounts of cellular proteins from both time intervals were mixed and used as control. For the control of inhibitor-treated cells, cells were treated with DMSO for 10 hours and cellular proteins were used as the control. * p < 0.05 (student t -test)
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    ATCC breast cell lines bt 474
    Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.
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    ATCC human breast cancer cell line bt474
    Phosphorylated levels of β1 downstream kinases are increased upon acquisition of resistance to lapatinib (L) . ( A ) Parental (P) <t>BT474</t> and ( B ) HCC1954 cells resistant to lapatinib (LRes), trastuzumab (TRes), and combination (LTRes) treatment strategies were developed by long-term exposure in 2D. Protein extracts were probed for β1, pHER2, pFAK, and pSrc, as well as totals.
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    ATCC human breast cancer cell line bt
    A. Effects of FS-93 on cell proliferation. <t>BT-474,</t> NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 72 h. Cell viability was assessed with SRB assay. Bars represent means±SD. B. Effects of FS-93 on degradation of client onco-proteins. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell lysates were prepared and analyzed with immunoblotting.
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    ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.

    Journal: mAbs

    Article Title: Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody

    doi: 10.1080/19420862.2018.1486946

    Figure Lengend Snippet: ADCC activity evaluation of anti-HER2 antibodies on four target cancer cells.

    Article Snippet: Human breast cancer cell line BT-474 (HTB-20), MDA-MB-175VII (HTB-25), SK-BR-3(HTB-30) and HCC1419(CRL-2326) are HER2-positive cell lines, which were obtained from the ATCC, and HCC1419 is trastuzumab-resistant human breast cancer cell line.

    Techniques: Activity Assay

    Effect of heregulin α1 (HRG α1) and p38 MAPK inhibitor (SB 203580) on Hsp27 phosphorylation . Cultures of cells from the BT474 breast cancer cell line were treated with HRG α1 for 10 and 30 min (A) or SB 203580 for 10 hours (B) and total cell lysates were extracted using M-PER reagent (Pierce). Equal amounts of proteins (20 μg) were separated by SDS-PAGE and transferred onto PVDF membrane. The membranes were blocked for 1 hour, followed by being probed with the respective primary antibodies (anti-Hsp27, anti-pSer 15 , anti-pSer 78 and anti-pSer 82 ), and HRP-conjugated secondary antibody. The signals were captured and their intensities were detected as described in Figure 2. The phosphorylation levels of pSer 15 , pSer 78 and pSer 82 were expressed as the ratios of intensity probed with phosphorylation site-specific antibody to the intensity probed with anti-Hsp27. Data with ± SD represents the average of triplicate experiments. C: control; SB: inhibitor SB203580. For the control of HRG-treated cells, untreated cells were cultured for 10 and 30 min and equal amounts of cellular proteins from both time intervals were mixed and used as control. For the control of inhibitor-treated cells, cells were treated with DMSO for 10 hours and cellular proteins were used as the control. * p < 0.05 (student t -test)

    Journal: Molecular Cancer

    Article Title: Phosphorylation of Ser 78 of Hsp27 correlated with HER-2/ neu status and lymph node positivity in breast cancer

    doi: 10.1186/1476-4598-6-52

    Figure Lengend Snippet: Effect of heregulin α1 (HRG α1) and p38 MAPK inhibitor (SB 203580) on Hsp27 phosphorylation . Cultures of cells from the BT474 breast cancer cell line were treated with HRG α1 for 10 and 30 min (A) or SB 203580 for 10 hours (B) and total cell lysates were extracted using M-PER reagent (Pierce). Equal amounts of proteins (20 μg) were separated by SDS-PAGE and transferred onto PVDF membrane. The membranes were blocked for 1 hour, followed by being probed with the respective primary antibodies (anti-Hsp27, anti-pSer 15 , anti-pSer 78 and anti-pSer 82 ), and HRP-conjugated secondary antibody. The signals were captured and their intensities were detected as described in Figure 2. The phosphorylation levels of pSer 15 , pSer 78 and pSer 82 were expressed as the ratios of intensity probed with phosphorylation site-specific antibody to the intensity probed with anti-Hsp27. Data with ± SD represents the average of triplicate experiments. C: control; SB: inhibitor SB203580. For the control of HRG-treated cells, untreated cells were cultured for 10 and 30 min and equal amounts of cellular proteins from both time intervals were mixed and used as control. For the control of inhibitor-treated cells, cells were treated with DMSO for 10 hours and cellular proteins were used as the control. * p < 0.05 (student t -test)

    Article Snippet: The human BT474 breast cancer cell line was obtained from American Type Culture Collection, and maintained in modified Dulbecco's medium (HybriCare) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere of 5% CO 2 in an incubator.

    Techniques: SDS Page, Cell Culture

    Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.

    Journal: BMC Cancer

    Article Title: CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers

    doi: 10.1186/1471-2407-11-418

    Figure Lengend Snippet: Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.

    Article Snippet: The breast cell lines BT-474, HCC1428, HCC1937, MCF7, MCF10A, MDA-MB-231, MDA-MB-361, MDA-MB-436, SK-BR-3 and ZR-75-1 were obtained from American Type Culture Collection (ATCC, Mannanas, VA).

    Techniques: Flow Cytometry, Expressing

    Phosphorylated levels of β1 downstream kinases are increased upon acquisition of resistance to lapatinib (L) . ( A ) Parental (P) BT474 and ( B ) HCC1954 cells resistant to lapatinib (LRes), trastuzumab (TRes), and combination (LTRes) treatment strategies were developed by long-term exposure in 2D. Protein extracts were probed for β1, pHER2, pFAK, and pSrc, as well as totals.

    Journal: Breast Cancer Research : BCR

    Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

    doi: 10.1186/bcr2936

    Figure Lengend Snippet: Phosphorylated levels of β1 downstream kinases are increased upon acquisition of resistance to lapatinib (L) . ( A ) Parental (P) BT474 and ( B ) HCC1954 cells resistant to lapatinib (LRes), trastuzumab (TRes), and combination (LTRes) treatment strategies were developed by long-term exposure in 2D. Protein extracts were probed for β1, pHER2, pFAK, and pSrc, as well as totals.

    Article Snippet: The human breast cancer cell line BT474 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained as previously described [ ].

    Techniques:

    β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor PF 573228 was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.

    Journal: Breast Cancer Research : BCR

    Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

    doi: 10.1186/bcr2936

    Figure Lengend Snippet: β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor PF 573228 was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.

    Article Snippet: The human breast cancer cell line BT474 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained as previously described [ ].

    Techniques: Staining, TUNEL Assay, Labeling, Western Blot

    Percent growth inhibition of cells in response to HER-targeted therapies

    Journal: Breast Cancer Research : BCR

    Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

    doi: 10.1186/bcr2936

    Figure Lengend Snippet: Percent growth inhibition of cells in response to HER-targeted therapies

    Article Snippet: The human breast cancer cell line BT474 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained as previously described [ ].

    Techniques: Inhibition

    HER2 and the β1 pathway play alternate roles in resistance to lapatinib-containing regimens, in comparison to trastuzumab . ( A ) Parental, LRes, and TRes cells were plated on lrECM in the presence of lapatinib and/or trastuzumab and assayed for response. ( B ) 3D cultures of parental, LRes, and TRes BT474 cells were harvested for protein and probed for phosphorylated and total HER receptors.

    Journal: Breast Cancer Research : BCR

    Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

    doi: 10.1186/bcr2936

    Figure Lengend Snippet: HER2 and the β1 pathway play alternate roles in resistance to lapatinib-containing regimens, in comparison to trastuzumab . ( A ) Parental, LRes, and TRes cells were plated on lrECM in the presence of lapatinib and/or trastuzumab and assayed for response. ( B ) 3D cultures of parental, LRes, and TRes BT474 cells were harvested for protein and probed for phosphorylated and total HER receptors.

    Article Snippet: The human breast cancer cell line BT474 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained as previously described [ ].

    Techniques:

    β1 inhibition impedes colony grow of BT474 LRes and LTRes cells, but not parental or TRes . ( A ) Parental, LRes, LTRes, and TRes cells were plated in lrECM, subjected to HER2 and/or β1 inhibitors on Day 0, and propagated for 10 to 12 days. ( B ) The Hs_ITGB1_5 siRNA was validated both in 2D and 3D (top), transfected at 40 nMsi into parental, LRes, LTRes, and TRes cells, which were then grown on lrECM for 10 days (bottom).

    Journal: Breast Cancer Research : BCR

    Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

    doi: 10.1186/bcr2936

    Figure Lengend Snippet: β1 inhibition impedes colony grow of BT474 LRes and LTRes cells, but not parental or TRes . ( A ) Parental, LRes, LTRes, and TRes cells were plated in lrECM, subjected to HER2 and/or β1 inhibitors on Day 0, and propagated for 10 to 12 days. ( B ) The Hs_ITGB1_5 siRNA was validated both in 2D and 3D (top), transfected at 40 nMsi into parental, LRes, LTRes, and TRes cells, which were then grown on lrECM for 10 days (bottom).

    Article Snippet: The human breast cancer cell line BT474 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained as previously described [ ].

    Techniques: Inhibition, Transfection

    A. Effects of FS-93 on cell proliferation. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 72 h. Cell viability was assessed with SRB assay. Bars represent means±SD. B. Effects of FS-93 on degradation of client onco-proteins. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell lysates were prepared and analyzed with immunoblotting.

    Journal: Oncoscience

    Article Title: FS-93, an Hsp90 inhibitor, induces G2/M arrest and apoptosis via the degradation of client proteins in oncogene addicted and derived resistant cancer cells

    doi:

    Figure Lengend Snippet: A. Effects of FS-93 on cell proliferation. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 72 h. Cell viability was assessed with SRB assay. Bars represent means±SD. B. Effects of FS-93 on degradation of client onco-proteins. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell lysates were prepared and analyzed with immunoblotting.

    Article Snippet: Human breast cancer cell line BT-474 was obtained from American Type Culture Collection (Manassas, VA).

    Techniques: Sulforhodamine B Assay, Western Blot

    A ., B. Effects of FS-93 on cell cycle distribution. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell cycle distribution was analyzed by FACS after propidium iodide staining. Representative images A. and quantification results B. were presented. Bars represent means±SD. C. Impacts of FS-93 on G2/M transition regulators. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h and cells lysates were immunoblotted with the indicated antibodies.

    Journal: Oncoscience

    Article Title: FS-93, an Hsp90 inhibitor, induces G2/M arrest and apoptosis via the degradation of client proteins in oncogene addicted and derived resistant cancer cells

    doi:

    Figure Lengend Snippet: A ., B. Effects of FS-93 on cell cycle distribution. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h. Cell cycle distribution was analyzed by FACS after propidium iodide staining. Representative images A. and quantification results B. were presented. Bars represent means±SD. C. Impacts of FS-93 on G2/M transition regulators. BT-474, NCI-H3122 and EBC-1 cells were treated with FS-93 at 25, 50 and 100nM or NVP-AUY922 at 100 nM for 24 h and cells lysates were immunoblotted with the indicated antibodies.

    Article Snippet: Human breast cancer cell line BT-474 was obtained from American Type Culture Collection (Manassas, VA).

    Techniques: Staining

    A. Effects of FS-93 on apoptosis induction. BT-474, NCI-H3122 and EBC-1 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h. Apoptosis was determined with annexin-V/PI assay. Bars represent means±SD. B. Impacts of FS-93 on apoptotic proteins. BT-474 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h and analyzed by immunoblotted with the indicated antibodies.

    Journal: Oncoscience

    Article Title: FS-93, an Hsp90 inhibitor, induces G2/M arrest and apoptosis via the degradation of client proteins in oncogene addicted and derived resistant cancer cells

    doi:

    Figure Lengend Snippet: A. Effects of FS-93 on apoptosis induction. BT-474, NCI-H3122 and EBC-1 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h. Apoptosis was determined with annexin-V/PI assay. Bars represent means±SD. B. Impacts of FS-93 on apoptotic proteins. BT-474 cells were treated with DMSO or FS-93 at 100 nM for 12, 24, 48 and 72h and analyzed by immunoblotted with the indicated antibodies.

    Article Snippet: Human breast cancer cell line BT-474 was obtained from American Type Culture Collection (Manassas, VA).

    Techniques: