bst ni  (New England Biolabs)


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  • 95
    Name:
    BstNI
    Description:
    BstNI 15 000 units
    Catalog Number:
    r0168l
    Price:
    249
    Size:
    15 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bst ni
    BstNI
    BstNI 15 000 units
    https://www.bioz.com/result/bst ni/product/New England Biolabs
    Average 95 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    bst ni - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis"

    Article Title: G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis

    Journal: British Journal of Cancer

    doi: 10.1038/sj.bjc.6601450

    PCR – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), Bst NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.
    Figure Legend Snippet: PCR – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), Bst NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.

    Techniques Used: Polymerase Chain Reaction, Mutagenesis, Marker, Amplification

    2) Product Images from "Rapid Concerted Evolution of Nuclear Ribosomal DNA in Two Tragopogon Allopolyploids of Recent and Recurrent Origin"

    Article Title: Rapid Concerted Evolution of Nuclear Ribosomal DNA in Two Tragopogon Allopolyploids of Recent and Recurrent Origin

    Journal: Genetics

    doi: 10.1534/genetics.104.032839

    35S rRNA gene expression in diploid and allotetraploid species of Tragopogon. Primary RNA transcripts were analyzed in the ITS-1 region by RT-PCR using Bst NI restriction site polymorphisms. The digestion products of PCR reactions were separated on a 7%
    Figure Legend Snippet: 35S rRNA gene expression in diploid and allotetraploid species of Tragopogon. Primary RNA transcripts were analyzed in the ITS-1 region by RT-PCR using Bst NI restriction site polymorphisms. The digestion products of PCR reactions were separated on a 7%

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction

    3) Product Images from "Selection of Recombinant Antibodies Specific for Pathogenic Streptococcus suis by Subtractive Phage Display"

    Article Title: Selection of Recombinant Antibodies Specific for Pathogenic Streptococcus suis by Subtractive Phage Display

    Journal: Infection and Immunity

    doi:

    Bst NI fingerprints of the inserts of phages selected against purified EF protein and S. suis cells. Individual clones were subjected to PCR and restricted with Bst NI. Lane 1, EF-specific clone E-H1; lane 2, EF-specific clone E-D9; lane 3, EF-specific clone E-H3; lane 4, S. suis -specific clone S-A7; lane 5, S. suis -specific clone S-B1; lane 6, S. suis -specific clone S-F9. The size of products is indicated in base pairs.
    Figure Legend Snippet: Bst NI fingerprints of the inserts of phages selected against purified EF protein and S. suis cells. Individual clones were subjected to PCR and restricted with Bst NI. Lane 1, EF-specific clone E-H1; lane 2, EF-specific clone E-D9; lane 3, EF-specific clone E-H3; lane 4, S. suis -specific clone S-A7; lane 5, S. suis -specific clone S-B1; lane 6, S. suis -specific clone S-F9. The size of products is indicated in base pairs.

    Techniques Used: Purification, Clone Assay, Polymerase Chain Reaction

    Bst NI and Ava II fingerprints of the inserts of EF-specific clone E-H1 and S. suis -specific clone Sub-B3. Individual clones were subjected to PCR and restricted with Bst NI and Ava II. Lane 1, EF specific-clone E-H1 restricted with Bst NI; lane 2, S. suis -specific clone Sub-B3 restricted with Bst NI; lane 3, EF-specific clone E-H1 restricted with Ava II; lane 4, S. suis -specific clone Sub-B3 restricted with Ava II. The size of products is indicated in base pairs.
    Figure Legend Snippet: Bst NI and Ava II fingerprints of the inserts of EF-specific clone E-H1 and S. suis -specific clone Sub-B3. Individual clones were subjected to PCR and restricted with Bst NI and Ava II. Lane 1, EF specific-clone E-H1 restricted with Bst NI; lane 2, S. suis -specific clone Sub-B3 restricted with Bst NI; lane 3, EF-specific clone E-H1 restricted with Ava II; lane 4, S. suis -specific clone Sub-B3 restricted with Ava II. The size of products is indicated in base pairs.

    Techniques Used: Antiviral Assay, Clone Assay, Polymerase Chain Reaction

    4) Product Images from "Genetic diversity among Plasmodium vivax isolates along the Thai–Myanmar border of Thailand"

    Article Title: Genetic diversity among Plasmodium vivax isolates along the Thai–Myanmar border of Thailand

    Journal: Malaria Journal

    doi: 10.1186/s12936-016-1136-6

    RFLP analysis of the Pvcsp fragments. In lanes with odd numbers , the fragments were digested with Alu I (an enzyme that cuts repeatedly in the VK210 repeat region). In lanes with even numbers , the fragments were digested with Bst NI (an enzyme that cuts repeatedly in the VK247 repeat region). Paired digestions from the fragments obtained from a single isolate are presented in lanes 1–2 (a VK210 variant), lanes 3–4 (a VK247 variant), and in lanes 5–6 and 7–8 in which mixed genotypes are present. A 100 bp ladder was used as molecular weight marker (M)
    Figure Legend Snippet: RFLP analysis of the Pvcsp fragments. In lanes with odd numbers , the fragments were digested with Alu I (an enzyme that cuts repeatedly in the VK210 repeat region). In lanes with even numbers , the fragments were digested with Bst NI (an enzyme that cuts repeatedly in the VK247 repeat region). Paired digestions from the fragments obtained from a single isolate are presented in lanes 1–2 (a VK210 variant), lanes 3–4 (a VK247 variant), and in lanes 5–6 and 7–8 in which mixed genotypes are present. A 100 bp ladder was used as molecular weight marker (M)

    Techniques Used: Variant Assay, Molecular Weight, Marker

    5) Product Images from "G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis"

    Article Title: G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis

    Journal: British Journal of Cancer

    doi: 10.1038/sj.bjc.6601450

    PCR – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), Bst NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.
    Figure Legend Snippet: PCR – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), Bst NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.

    Techniques Used: Polymerase Chain Reaction, Mutagenesis, Marker, Amplification

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish
    Article Snippet: .. After digestion with Bst N I (restriction enzyme cutting site in the spacer of foxl2a ) or Bsr D I (restriction enzyme cutting site in the spacer of foxl2b ) (New England Biolabs, Beverly, MA) respectively at 60° and 65° for 2 hr, the uncleaved DNA fragments were separated by gel electrophoresis and cloned into pMD-18T vector (Promega). .. Sequence alignments were generated to analyze whether or not the gDNA fragments from embryos injected with TALEN mRNAs were mutated.

    Amplification:

    Article Title: Diagnostic Molecular Markers for Phosphine Resistance in U.S. Populations of Tribolium castaneum and Rhyzopertha dominica
    Article Snippet: .. The amplified 368bp PCR product from T . castaneum and the 375bp PCR product from R . dominica were subjected to separate restriction enzyme digestion with either MboI or BstNI (New England Biolabs, MA USA) in a 10μl reaction containing 8μl of PCR product, 1μl reaction buffer and 1U restriction enzyme. .. The reaction was then incubated at 37°C for MboI or 60°C for BstNI, respectively for 15min according to manufacturer’s instruction.

    Agarose Gel Electrophoresis:

    Article Title: Development and Evaluation of a Real-Time PCR Assay for Detection of Klebsiella pneumoniae Carbapenemase Genes ▿
    Article Snippet: .. The following cycling conditions were used: 95o for 2 min, followed by 35 cycles of 94°C for 2 s, 62°C for 10 s, and 72°C for 15 s. The amplicons from KPC-positive samples were then digested at 48°C for 1 h in RsaI and BstNI (New England Biolabs, Ipswitch, MA) using NEB buffer 2 (New England Biolabs) and electrophoresed on a 2% agarose gel to differentiate bla KPC-1 , bla KPC-2 , and bla KPC-3 . ..

    Clone Assay:

    Article Title: Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish
    Article Snippet: .. After digestion with Bst N I (restriction enzyme cutting site in the spacer of foxl2a ) or Bsr D I (restriction enzyme cutting site in the spacer of foxl2b ) (New England Biolabs, Beverly, MA) respectively at 60° and 65° for 2 hr, the uncleaved DNA fragments were separated by gel electrophoresis and cloned into pMD-18T vector (Promega). .. Sequence alignments were generated to analyze whether or not the gDNA fragments from embryos injected with TALEN mRNAs were mutated.

    Isolation:

    Article Title: Ligation of high-melting-temperature 'clamp' sequence extends the scanning range of rare point-mutational analysis by constant denaturant capillary electrophoresis (CDCE) to most of the human genome
    Article Snippet: .. Restriction digestion by BstNI and DraI (New England Biolabs, Beverly, MA) was performed with the samples of genomic DNA to liberate the HPRT target-embedded fragment of 438 bp suitable for target isolation by probe-target hybridization coupled with a biotin–streptavidin capture system ( ). ..

    Size-exclusion Chromatography:

    Article Title: Rapid Concerted Evolution of Nuclear Ribosomal DNA in Two Tragopogon Allopolyploids of Recent and Recurrent Origin
    Article Snippet: .. Cycling conditions were as follows: initial denaturation step (92°, 180 sec) and 35 cycles of 92° for 20 sec, 57° for 30 sec, and 72° for 30 sec, followed by a final 72° extension for 10 min. To discriminate among ITS-1 variants, the PCR products were digested with Bst NI (New England Biolabs, Beverly, MA), which produces species-specific DNA fragments for each diploid progenitor ( ); the resulting fragments were separated on a 7% polyacrylamide gel. .. The gel was stained after electrophoresis with ethidium bromide and photographed.

    Incubation:

    Article Title: G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis
    Article Snippet: .. The digestion reactions contained 10 μ l of PCR product, 0.5 μ l of Bst NI (5 U; New England Biolabs, Beverly, MA, USA), 2 μ l of 10 × NEBuffer 2 (supplied with the enzyme) and 0.2 μ l bovine serum albumin (100 mg l−1 ) in a final volume of 20 μ l. These components were incubated for 60 min at 60°C. .. After the reaction ended, 10 μ l of the PCR mixture were mixed with a loading buffer and electrophoresed in a 4% agarose 1000® gel (Life Technologies, Carlsbad, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Selection of Recombinant Antibodies Specific for Pathogenic Streptococcus suis by Subtractive Phage Display
    Article Snippet: .. PCR products were digested with Bst NI (New England Biolabs, Beverly, Mass.) or Ava II (Promega, Madison, Wis.). .. The restriction mixture, containing 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , 1 mM dithiothreitol, and 4 U of Bst NI or Ava II, was added to the PCR mixture in a 1:1 ratio.

    Article Title: Diagnostic Molecular Markers for Phosphine Resistance in U.S. Populations of Tribolium castaneum and Rhyzopertha dominica
    Article Snippet: .. The amplified 368bp PCR product from T . castaneum and the 375bp PCR product from R . dominica were subjected to separate restriction enzyme digestion with either MboI or BstNI (New England Biolabs, MA USA) in a 10μl reaction containing 8μl of PCR product, 1μl reaction buffer and 1U restriction enzyme. .. The reaction was then incubated at 37°C for MboI or 60°C for BstNI, respectively for 15min according to manufacturer’s instruction.

    Article Title: Genetic diversity among Plasmodium vivax isolates along the Thai–Myanmar border of Thailand
    Article Snippet: .. Subsequently, 20 µL of the second PCR products were separately digested by restriction enzymes Alu I and Bst NI (New England Biolabs Inc., UK) according to the manufacture specification for 2 h in a total volume of 30 µL. .. The DNA fragments were separated by electrophoresis on 3 % agarose gel and visualized under UV illumination after ethidium bromide staining.

    Article Title: Rapid Concerted Evolution of Nuclear Ribosomal DNA in Two Tragopogon Allopolyploids of Recent and Recurrent Origin
    Article Snippet: .. Cycling conditions were as follows: initial denaturation step (92°, 180 sec) and 35 cycles of 92° for 20 sec, 57° for 30 sec, and 72° for 30 sec, followed by a final 72° extension for 10 min. To discriminate among ITS-1 variants, the PCR products were digested with Bst NI (New England Biolabs, Beverly, MA), which produces species-specific DNA fragments for each diploid progenitor ( ); the resulting fragments were separated on a 7% polyacrylamide gel. .. The gel was stained after electrophoresis with ethidium bromide and photographed.

    Article Title: G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis
    Article Snippet: .. The digestion reactions contained 10 μ l of PCR product, 0.5 μ l of Bst NI (5 U; New England Biolabs, Beverly, MA, USA), 2 μ l of 10 × NEBuffer 2 (supplied with the enzyme) and 0.2 μ l bovine serum albumin (100 mg l−1 ) in a final volume of 20 μ l. These components were incubated for 60 min at 60°C. .. After the reaction ended, 10 μ l of the PCR mixture were mixed with a loading buffer and electrophoresed in a 4% agarose 1000® gel (Life Technologies, Carlsbad, CA, USA).

    Antiviral Assay:

    Article Title: Selection of Recombinant Antibodies Specific for Pathogenic Streptococcus suis by Subtractive Phage Display
    Article Snippet: .. PCR products were digested with Bst NI (New England Biolabs, Beverly, Mass.) or Ava II (Promega, Madison, Wis.). .. The restriction mixture, containing 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , 1 mM dithiothreitol, and 4 U of Bst NI or Ava II, was added to the PCR mixture in a 1:1 ratio.

    Plasmid Preparation:

    Article Title: Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish
    Article Snippet: .. After digestion with Bst N I (restriction enzyme cutting site in the spacer of foxl2a ) or Bsr D I (restriction enzyme cutting site in the spacer of foxl2b ) (New England Biolabs, Beverly, MA) respectively at 60° and 65° for 2 hr, the uncleaved DNA fragments were separated by gel electrophoresis and cloned into pMD-18T vector (Promega). .. Sequence alignments were generated to analyze whether or not the gDNA fragments from embryos injected with TALEN mRNAs were mutated.

    Hybridization:

    Article Title: Ligation of high-melting-temperature 'clamp' sequence extends the scanning range of rare point-mutational analysis by constant denaturant capillary electrophoresis (CDCE) to most of the human genome
    Article Snippet: .. Restriction digestion by BstNI and DraI (New England Biolabs, Beverly, MA) was performed with the samples of genomic DNA to liberate the HPRT target-embedded fragment of 438 bp suitable for target isolation by probe-target hybridization coupled with a biotin–streptavidin capture system ( ). ..

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    New England Biolabs bst ni
    <t>PCR</t> – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), <t>Bst</t> NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.
    Bst Ni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst ni/product/New England Biolabs
    Average 95 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    bst ni - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    90
    New England Biolabs enzymes bst ni
    A chromatogram screenshot of the DNA sequence (partial) of <t>MdACS3a</t> encompassing SNPs G 866 /T 866 and C 870 /T 870 in six apple cultivars—‘Florina’, ’Fuji red sport’, ‘Gala’, ‘Golden Delicious’ and ‘Granny Smith’. The oval circles in brown and red indicate the homozygous or heterozygous status at the 866th and 870th nucleotides in the coding sequence of MdACS3a , respectively. The recognition sites of restriction enzymes <t>Bst</t> NI and Taq α I are provided to show that the mutation from G 866 to T 866 abolishes the restriction site of Bst NI while the mutation from C 870 to T 870 gives rise to a restriction site for Taq α I. The right panel shows allelotypes of MdACS3a as represented by the SNP alleles, where G 866 stands for allele MdACS3a (wild type), T 866 for MdACS3a-G289V (functional null allele), C 870 also for allele MdACS3a and T 870 for Mdacs3a (transcriptional null allele).
    Enzymes Bst Ni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymes bst ni/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzymes bst ni - by Bioz Stars, 2020-08
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    85
    New England Biolabs restriction enzymes bst ni
    Restriction site analyses of exons 6 and 7 of the CD18 genes of AW (LAD patient) and her parents MW (father) and TW (mother). (a) The 342-bp fragments spanning exon 6 were either left undigested (U) or digested with <t>Bst</t> NI (B) and the samples were run in a 3% Nusieve agarose gel. (b) The 175-bp fragments spanning exon 7 were similarly analysed with the restriction enzyme <t>Eag</t> I (lanes marked E). SL is a normal unrelated individual, and BB and YM are LAD patients who are homozygous for the G284S mutation.
    Restriction Enzymes Bst Ni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bst ni/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bst ni - by Bioz Stars, 2020-08
    85/100 stars
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    Image Search Results


    PCR – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), Bst NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.

    Journal: British Journal of Cancer

    Article Title: G388R mutation of the FGFR4 gene is not relevant to breast cancer prognosis

    doi: 10.1038/sj.bjc.6601450

    Figure Lengend Snippet: PCR – RFLP analysis of FGFR4 gene G388R mutation. Lane 1, Hae III-digested pBR322 size marker; lane 2, amplification control; lane 3, wild-type control (Gly/Gly), Bst NI – digested; lane 4, heterozygote carrier (Gly/Arg), Bst NI – digested; lane 5, homozygote carrier (Arg/Arg), Bst NI – digested.

    Article Snippet: The digestion reactions contained 10 μ l of PCR product, 0.5 μ l of Bst NI (5 U; New England Biolabs, Beverly, MA, USA), 2 μ l of 10 × NEBuffer 2 (supplied with the enzyme) and 0.2 μ l bovine serum albumin (100 mg l−1 ) in a final volume of 20 μ l. These components were incubated for 60 min at 60°C.

    Techniques: Polymerase Chain Reaction, Mutagenesis, Marker, Amplification

    35S rRNA gene expression in diploid and allotetraploid species of Tragopogon. Primary RNA transcripts were analyzed in the ITS-1 region by RT-PCR using Bst NI restriction site polymorphisms. The digestion products of PCR reactions were separated on a 7%

    Journal: Genetics

    Article Title: Rapid Concerted Evolution of Nuclear Ribosomal DNA in Two Tragopogon Allopolyploids of Recent and Recurrent Origin

    doi: 10.1534/genetics.104.032839

    Figure Lengend Snippet: 35S rRNA gene expression in diploid and allotetraploid species of Tragopogon. Primary RNA transcripts were analyzed in the ITS-1 region by RT-PCR using Bst NI restriction site polymorphisms. The digestion products of PCR reactions were separated on a 7%

    Article Snippet: Cycling conditions were as follows: initial denaturation step (92°, 180 sec) and 35 cycles of 92° for 20 sec, 57° for 30 sec, and 72° for 30 sec, followed by a final 72° extension for 10 min. To discriminate among ITS-1 variants, the PCR products were digested with Bst NI (New England Biolabs, Beverly, MA), which produces species-specific DNA fragments for each diploid progenitor ( ); the resulting fragments were separated on a 7% polyacrylamide gel.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction

    A chromatogram screenshot of the DNA sequence (partial) of MdACS3a encompassing SNPs G 866 /T 866 and C 870 /T 870 in six apple cultivars—‘Florina’, ’Fuji red sport’, ‘Gala’, ‘Golden Delicious’ and ‘Granny Smith’. The oval circles in brown and red indicate the homozygous or heterozygous status at the 866th and 870th nucleotides in the coding sequence of MdACS3a , respectively. The recognition sites of restriction enzymes Bst NI and Taq α I are provided to show that the mutation from G 866 to T 866 abolishes the restriction site of Bst NI while the mutation from C 870 to T 870 gives rise to a restriction site for Taq α I. The right panel shows allelotypes of MdACS3a as represented by the SNP alleles, where G 866 stands for allele MdACS3a (wild type), T 866 for MdACS3a-G289V (functional null allele), C 870 also for allele MdACS3a and T 870 for Mdacs3a (transcriptional null allele).

    Journal: Horticulture Research

    Article Title: Assessing the allelotypic effect of two aminocyclopropane carboxylic acid synthase-encoding genes MdACS1 and MdACS3a on fruit ethylene production and softening in Malus

    doi: 10.1038/hortres.2016.24

    Figure Lengend Snippet: A chromatogram screenshot of the DNA sequence (partial) of MdACS3a encompassing SNPs G 866 /T 866 and C 870 /T 870 in six apple cultivars—‘Florina’, ’Fuji red sport’, ‘Gala’, ‘Golden Delicious’ and ‘Granny Smith’. The oval circles in brown and red indicate the homozygous or heterozygous status at the 866th and 870th nucleotides in the coding sequence of MdACS3a , respectively. The recognition sites of restriction enzymes Bst NI and Taq α I are provided to show that the mutation from G 866 to T 866 abolishes the restriction site of Bst NI while the mutation from C 870 to T 870 gives rise to a restriction site for Taq α I. The right panel shows allelotypes of MdACS3a as represented by the SNP alleles, where G 866 stands for allele MdACS3a (wild type), T 866 for MdACS3a-G289V (functional null allele), C 870 also for allele MdACS3a and T 870 for Mdacs3a (transcriptional null allele).

    Article Snippet: To detect alleles MdACS3a-G289V and Mdacs3a , the PCR products were restricted with enzymes Bst NI and Taq α I (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instruction, respectively.

    Techniques: Sequencing, Mutagenesis, Functional Assay

    Agarose gel analyses of markers ACS1 ( a ), CAPS 866 ( b ) and CAPS 870 ( c ). For marker ACS1, the PCR products amplified by primers ACS1–5F/R were directly analyzed. Allelotypes MdACS1-1/1 , MdACS1–2/2 and MdACS1-1/2 are denoted with ‘1/1’, ‘2/2’ and ‘1/2’, respectively. For marker CAPS 866 , the PCR products were first amplified by primers ACS3a-289F/R and then digested with enzyme Bst NI, which restricts the MdACS3a (G 866 ) allele into the two lower bands. Allelotypes MdACS3a / MdACS3a (G 866 /G 866 ), MdACS3a / MdACS3a-G289V (G 866 /T 866 ) and MdACS3a-G289V/G289V (T 866 /T 866 ) are noted with ‘G/G’, ‘G/T’ and ‘T/T’, respectively. For marker CAPS 870 , enzyme Taq α I restricts the Mdacs3a (T 870 ) allele into the two lower bands. Allelotypes MdACS3a / MdACS3a (C 870 /C 870 ), MdACS3a / mdacs3a (C 870 /T 870 ) and mdacs3a/mdacs3a (T 870 /T 870 ) are noted with ‘C/C’, ‘C/T’ and ‘T/T’, respectively.

    Journal: Horticulture Research

    Article Title: Assessing the allelotypic effect of two aminocyclopropane carboxylic acid synthase-encoding genes MdACS1 and MdACS3a on fruit ethylene production and softening in Malus

    doi: 10.1038/hortres.2016.24

    Figure Lengend Snippet: Agarose gel analyses of markers ACS1 ( a ), CAPS 866 ( b ) and CAPS 870 ( c ). For marker ACS1, the PCR products amplified by primers ACS1–5F/R were directly analyzed. Allelotypes MdACS1-1/1 , MdACS1–2/2 and MdACS1-1/2 are denoted with ‘1/1’, ‘2/2’ and ‘1/2’, respectively. For marker CAPS 866 , the PCR products were first amplified by primers ACS3a-289F/R and then digested with enzyme Bst NI, which restricts the MdACS3a (G 866 ) allele into the two lower bands. Allelotypes MdACS3a / MdACS3a (G 866 /G 866 ), MdACS3a / MdACS3a-G289V (G 866 /T 866 ) and MdACS3a-G289V/G289V (T 866 /T 866 ) are noted with ‘G/G’, ‘G/T’ and ‘T/T’, respectively. For marker CAPS 870 , enzyme Taq α I restricts the Mdacs3a (T 870 ) allele into the two lower bands. Allelotypes MdACS3a / MdACS3a (C 870 /C 870 ), MdACS3a / mdacs3a (C 870 /T 870 ) and mdacs3a/mdacs3a (T 870 /T 870 ) are noted with ‘C/C’, ‘C/T’ and ‘T/T’, respectively.

    Article Snippet: To detect alleles MdACS3a-G289V and Mdacs3a , the PCR products were restricted with enzymes Bst NI and Taq α I (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instruction, respectively.

    Techniques: Agarose Gel Electrophoresis, Marker, Polymerase Chain Reaction, Amplification

    Restriction site analyses of exons 6 and 7 of the CD18 genes of AW (LAD patient) and her parents MW (father) and TW (mother). (a) The 342-bp fragments spanning exon 6 were either left undigested (U) or digested with Bst NI (B) and the samples were run in a 3% Nusieve agarose gel. (b) The 175-bp fragments spanning exon 7 were similarly analysed with the restriction enzyme Eag I (lanes marked E). SL is a normal unrelated individual, and BB and YM are LAD patients who are homozygous for the G284S mutation.

    Journal: Clinical and Experimental Immunology

    Article Title: A novel point mutation in CD18 causing the expression of dysfunctional CD11/CD18 leucocyte integrins in a patient with leucocyte adhesion deficiency (LAD)

    doi: 10.1046/j.1365-2249.2000.01277.x

    Figure Lengend Snippet: Restriction site analyses of exons 6 and 7 of the CD18 genes of AW (LAD patient) and her parents MW (father) and TW (mother). (a) The 342-bp fragments spanning exon 6 were either left undigested (U) or digested with Bst NI (B) and the samples were run in a 3% Nusieve agarose gel. (b) The 175-bp fragments spanning exon 7 were similarly analysed with the restriction enzyme Eag I (lanes marked E). SL is a normal unrelated individual, and BB and YM are LAD patients who are homozygous for the G284S mutation.

    Article Snippet: The restriction enzymes Bst NI and Eag I were purchased from New England BioLabs (Hitchin, UK).

    Techniques: Agarose Gel Electrophoresis, Mutagenesis