bsshii  (New England Biolabs)


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  • 95
    Name:
    BssHII
    Description:
    BssHII 2 500 units
    Catalog Number:
    r0199l
    Price:
    282
    Size:
    2 500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bsshii
    BssHII
    BssHII 2 500 units
    https://www.bioz.com/result/bsshii/product/New England Biolabs
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bsshii - by Bioz Stars, 2020-07
    95/100 stars

    Images

    1) Product Images from "One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome"

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

    Journal:

    doi: 10.1073/pnas.0811011106

    Validation of an intact M. genitalium genome by restriction analysis. ( A ) Diagram of the EagI (red), BssHII (blue), and AatII (green) restriction fragments expected for a complete and proper assembly of the synthetic JCVI-1.1 M. genitalium genome. The
    Figure Legend Snippet: Validation of an intact M. genitalium genome by restriction analysis. ( A ) Diagram of the EagI (red), BssHII (blue), and AatII (green) restriction fragments expected for a complete and proper assembly of the synthetic JCVI-1.1 M. genitalium genome. The

    Techniques Used:

    2) Product Images from "Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages"

    Article Title: Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01233-12

    Restriction pattern of the three bacteriophages A, B, and C. Genomic DNA digested by HindIII or BssHII was run on an agarose gel. M, size marker.
    Figure Legend Snippet: Restriction pattern of the three bacteriophages A, B, and C. Genomic DNA digested by HindIII or BssHII was run on an agarose gel. M, size marker.

    Techniques Used: Agarose Gel Electrophoresis, Marker

    Related Articles

    Clone Assay:

    Article Title: Novel Human Polyomavirus Noncoding Control Regions Differ in Bidirectional Gene Expression according to Host Cell, Large T-Antigen Expression, and Clinically Occurring Rearrangements
    Article Snippet: .. The HPyV NCCRs were chemically synthesized in pUC57 (Eurogentec S.A, Belgium) , excised using the restriction enzymes BssHII and MluI (New England BioLabs, England), and cloned into the corresponding restriction sites of pRG13D12. .. HPyV NCCR constructs were verified by Sanger sequencing for correct NCCR sequences and orientations using the 3130 genetic analyzer (Applied Biosystems, Switzerland).

    Synthesized:

    Article Title: Novel Human Polyomavirus Noncoding Control Regions Differ in Bidirectional Gene Expression according to Host Cell, Large T-Antigen Expression, and Clinically Occurring Rearrangements
    Article Snippet: .. The HPyV NCCRs were chemically synthesized in pUC57 (Eurogentec S.A, Belgium) , excised using the restriction enzymes BssHII and MluI (New England BioLabs, England), and cloned into the corresponding restriction sites of pRG13D12. .. HPyV NCCR constructs were verified by Sanger sequencing for correct NCCR sequences and orientations using the 3130 genetic analyzer (Applied Biosystems, Switzerland).

    Isolation:

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: .. Probes corresponding to five unique E.cuniculi genes isolated in our laboratory ( , ; C.Biderre, personal communication), were hybridised to Bss HII- and Mlu I-KARD-PFGE gels. ..

    Methylation:

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: .. This low frequency might also be explained by a DNA modification to which both Bss HII and Mlu I are sensitive, e.g. cytosine methylation as in higher eukaryotes. .. If DNA methylation occurs in E.cuniculi , differences in the methylation pattern of two homologous chromosomes should lead to differentially migrated fragments in KARD-PFGE gels.

    Labeling:

    Article Title: Murine Cytomegalovirus CC Chemokine Homolog MCK-2 (m131-129) Is a Determinant of Dissemination That Increases Inflammation at Initial Sites of Infection
    Article Snippet: .. Virion DNA (0.5 to 1.0 μg) was digested with Hin dIII, Bss HII, Afl II, or Spe I (New England Biolabs, Beverly, Mass.) and end labeled in the presence of 2.5 μCi of [α-32 P]dCTP (Amersham), 125 μM each dATP, dGTP, and dTTP, and 0.5 U of Klenow polymerase (Roche, Indianapolis, Ind.) for 15 min at room temperature in 20 μl of restriction enzyme buffer. .. Restriction fragments were separated on a 0.6% agarose gel, which was fixed in 95% ethanol and vacuum dried at 80°C, followed by autoradiography.

    other:

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: In order to place the Bss HII and Mlu I restriction sites on each chromosome, we developed a mapping procedure applied to individual chromosomes (DDIC-PFGE) that can be viewed as the counterpart of 2D-PFGE in bacterial genomics ( ).

    Article Title: Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients
    Article Snippet: In comparison, restriction endonuclease analysis of the genome (REAG) from the 49 isolates of 22 patients yielded 24 patterns after digestion with Sfi I ( ) and 25 patterns after digestion with Bss HII ( ).

    Modification:

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: .. This low frequency might also be explained by a DNA modification to which both Bss HII and Mlu I are sensitive, e.g. cytosine methylation as in higher eukaryotes. .. If DNA methylation occurs in E.cuniculi , differences in the methylation pattern of two homologous chromosomes should lead to differentially migrated fragments in KARD-PFGE gels.

    Plasmid Preparation:

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase
    Article Snippet: .. According to the pBluescript map, we would expect to see the DNA fragment next to the pBluescript vector at the T3 promoter site in pBluMH to be about 0.1 kb smaller when double-digested with Bss HII + Pst I than when digested with Bss HII alone. .. The Southern hybridization results show that the 1.1-kb fragment from Bss HII digestion corresponds to a 1-kb fragment in the Bss HII + Pst I double-digestion.

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  • 95
    New England Biolabs bss hii
    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), <t>Bss</t> <t>HII</t> (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Bss Hii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bss hii/product/New England Biolabs
    Average 95 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    bss hii - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    doi:

    Figure Lengend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Article Snippet: According to the pBluescript map, we would expect to see the DNA fragment next to the pBluescript vector at the T3 promoter site in pBluMH to be about 0.1 kb smaller when double-digested with Bss HII + Pst I than when digested with Bss HII alone.

    Techniques: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    doi:

    Figure Lengend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Article Snippet: According to the pBluescript map, we would expect to see the DNA fragment next to the pBluescript vector at the T3 promoter site in pBluMH to be about 0.1 kb smaller when double-digested with Bss HII + Pst I than when digested with Bss HII alone.

    Techniques: Sequencing

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Journal: Nucleic Acids Research

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    doi:

    Figure Lengend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Article Snippet: This low frequency might also be explained by a DNA modification to which both Bss HII and Mlu I are sensitive, e.g. cytosine methylation as in higher eukaryotes.

    Techniques: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Journal: Nucleic Acids Research

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    doi:

    Figure Lengend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Article Snippet: This low frequency might also be explained by a DNA modification to which both Bss HII and Mlu I are sensitive, e.g. cytosine methylation as in higher eukaryotes.

    Techniques: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Journal: Nucleic Acids Research

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    doi:

    Figure Lengend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Article Snippet: This low frequency might also be explained by a DNA modification to which both Bss HII and Mlu I are sensitive, e.g. cytosine methylation as in higher eukaryotes.

    Techniques: Hybridization, Marker

    Rearranged BKPyV Dunlop NCCR showed higher EVGR expression than did BKPyVww NCCR in HEK293 cells. (A) Schematic representation of the HPyV genome: noncoding control region (NCCR); early viral gene region (EVGR, in red) encoding large and small T antigens (Tags), alternative spliced Tags; microRNAs (blue arrow); late viral gene region (LVGR) encoding structural proteins (Vp1, Vp2, and Vp3) and the agnoprotein (agno) only in BKPyV and JCPyV. (B) Representation of the bidirectional reporter vector pRG13D12, containing the following: NCCR (in gray) in the early to late orientation cloned via restriction sites MluI and BssHII; the red fluorescence protein dsRed2, used as a marker of EVGR expression; the enhanced green fluorescence protein, EGFP, in the opposite orientation, used as a marker of LVGR expression; SV40 polyadenylation signals [SV40 poly(A)] for the dsRed2 and EGFP expression cassette; E1 ori for bacterial plasmid replication; the ampicillin-resistant gene (Amp) for selecting Escherichia coli transformants. (C) Flow cytometry of HEK293 cells 2 dpt with the pRG13D12 reporter vector alone or containing the NCCR of the archetype BKPyVww, the BKPyV(DUN), or the BKPyV(DUN-R) in the reverse orientation. x axis, EGFP fluorescence; y axis, dsRed2 fluorescence; 10,000 control transfected cells were gated for the live gate, while 5,000 transfected cells were gated for the P3 (Q1, Q2, and Q4) gate. Q1, Q4, and Q2 depict cells expressing red fluorescence, green fluorescence, and both, respectively. Ex, excitation wavelength; Em, emission wavelength. (D) Quantification of cells: red bars, sum of red cells (Q1 + Q2); green bars, sum of green cells (Q2 + Q4); yellow bars, red- and green-fluorescence double-positive cells (Q2); black bars, nonfluorescent cells (Q3, negative). Means with standard deviations (SD) from three independent replicates are shown. (E) Normalized mean fluorescence intensity (MFI). The weighted MFI was calculated for each measurement (see formulas in Materials and Methods); late expression was normalized to BKPyVww NCCR (green MFI was set as 100), while early expression was normalized to BKPyVww NCCR (red MFI was set as 1). Means with SD from three independent replicates are shown.

    Journal: Journal of Virology

    Article Title: Novel Human Polyomavirus Noncoding Control Regions Differ in Bidirectional Gene Expression according to Host Cell, Large T-Antigen Expression, and Clinically Occurring Rearrangements

    doi: 10.1128/JVI.02231-17

    Figure Lengend Snippet: Rearranged BKPyV Dunlop NCCR showed higher EVGR expression than did BKPyVww NCCR in HEK293 cells. (A) Schematic representation of the HPyV genome: noncoding control region (NCCR); early viral gene region (EVGR, in red) encoding large and small T antigens (Tags), alternative spliced Tags; microRNAs (blue arrow); late viral gene region (LVGR) encoding structural proteins (Vp1, Vp2, and Vp3) and the agnoprotein (agno) only in BKPyV and JCPyV. (B) Representation of the bidirectional reporter vector pRG13D12, containing the following: NCCR (in gray) in the early to late orientation cloned via restriction sites MluI and BssHII; the red fluorescence protein dsRed2, used as a marker of EVGR expression; the enhanced green fluorescence protein, EGFP, in the opposite orientation, used as a marker of LVGR expression; SV40 polyadenylation signals [SV40 poly(A)] for the dsRed2 and EGFP expression cassette; E1 ori for bacterial plasmid replication; the ampicillin-resistant gene (Amp) for selecting Escherichia coli transformants. (C) Flow cytometry of HEK293 cells 2 dpt with the pRG13D12 reporter vector alone or containing the NCCR of the archetype BKPyVww, the BKPyV(DUN), or the BKPyV(DUN-R) in the reverse orientation. x axis, EGFP fluorescence; y axis, dsRed2 fluorescence; 10,000 control transfected cells were gated for the live gate, while 5,000 transfected cells were gated for the P3 (Q1, Q2, and Q4) gate. Q1, Q4, and Q2 depict cells expressing red fluorescence, green fluorescence, and both, respectively. Ex, excitation wavelength; Em, emission wavelength. (D) Quantification of cells: red bars, sum of red cells (Q1 + Q2); green bars, sum of green cells (Q2 + Q4); yellow bars, red- and green-fluorescence double-positive cells (Q2); black bars, nonfluorescent cells (Q3, negative). Means with standard deviations (SD) from three independent replicates are shown. (E) Normalized mean fluorescence intensity (MFI). The weighted MFI was calculated for each measurement (see formulas in Materials and Methods); late expression was normalized to BKPyVww NCCR (green MFI was set as 100), while early expression was normalized to BKPyVww NCCR (red MFI was set as 1). Means with SD from three independent replicates are shown.

    Article Snippet: The HPyV NCCRs were chemically synthesized in pUC57 (Eurogentec S.A, Belgium) , excised using the restriction enzymes BssHII and MluI (New England BioLabs, England), and cloned into the corresponding restriction sites of pRG13D12.

    Techniques: Expressing, Plasmid Preparation, Clone Assay, Fluorescence, Marker, Flow Cytometry, Cytometry, Transfection