bss hii  (New England Biolabs)


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    Name:
    BssHII
    Description:

    Catalog Number:
    R0199
    Price:
    287
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    2500 units
    Buy from Supplier


    Structured Review

    New England Biolabs bss hii
    BssHII

    https://www.bioz.com/result/bss hii/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bss hii - by Bioz Stars, 2021-07
    86/100 stars

    Images

    1) Product Images from "Murine Cytomegalovirus CC Chemokine Homolog MCK-2 (m131-129) Is a Determinant of Dissemination That Increases Inflammation at Initial Sites of Infection"

    Article Title: Murine Cytomegalovirus CC Chemokine Homolog MCK-2 (m131-129) Is a Determinant of Dissemination That Increases Inflammation at Initial Sites of Infection

    Journal: Journal of Virology

    doi: 10.1128/JVI.75.20.9966-9976.2001

    Restriction digestion analysis of RM4511 DNA. (A) Detection of the RM4511 mck mutation by Bbr PI digestion. Autoradiograph of Bbr PI-digested (Roche) virion DNA from parental RM427 + , RM4503, and two isolates of RM4511 (RM4511.1 and RM4511.2) following electrophoretic separation on a 1% agarose gel and hybridization with a Hin dIII/ Afl II fragment mck probe. (B) Detection of the EGFP-puro insert in RM4503 and RM4511. Autoradiograph of electrophoretically separated, Bss HII-digested 32 P-end-labeled DNA fragments. (C) Autoradiograph of electrophoretically separated 32 P-end-labeled Hin dIII fragments of DNA from RM427 + , RM4503, and two isolates of RM4511.
    Figure Legend Snippet: Restriction digestion analysis of RM4511 DNA. (A) Detection of the RM4511 mck mutation by Bbr PI digestion. Autoradiograph of Bbr PI-digested (Roche) virion DNA from parental RM427 + , RM4503, and two isolates of RM4511 (RM4511.1 and RM4511.2) following electrophoretic separation on a 1% agarose gel and hybridization with a Hin dIII/ Afl II fragment mck probe. (B) Detection of the EGFP-puro insert in RM4503 and RM4511. Autoradiograph of electrophoretically separated, Bss HII-digested 32 P-end-labeled DNA fragments. (C) Autoradiograph of electrophoretically separated 32 P-end-labeled Hin dIII fragments of DNA from RM427 + , RM4503, and two isolates of RM4511.

    Techniques Used: Mutagenesis, Autoradiography, Agarose Gel Electrophoresis, Hybridization, Labeling

    2) Product Images from "Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase"

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Figure Legend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Techniques Used: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.
    Figure Legend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Techniques Used: Sequencing

    3) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    4) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    5) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    6) Product Images from "Nuclear Progestin Receptor (Pgr) Knockouts in Zebrafish Demonstrate Role for Pgr in Ovulation but Not in Rapid Non-Genomic Steroid Mediated Meiosis Resumption"

    Article Title: Nuclear Progestin Receptor (Pgr) Knockouts in Zebrafish Demonstrate Role for Pgr in Ovulation but Not in Rapid Non-Genomic Steroid Mediated Meiosis Resumption

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2015.00037

    Genomic DNA sequence of first exon (in upper case) and flanking intron regions (in lower case) of nuclear progestin receptor (nPR or pgr ) . First and second TALEN targeting sites are highlighted either in green or yellow, respectively. Translation start site (ATG) and restriction enzyme recognition sites ( Bss HII: GCGCGC; Sac II, CCGCGG) are indicated by box. Forward and reverse PCR primers for amplification of genomic region including TALEN targeting sites are underlined. The numbers on the far right of the figure indicate the positions of the nucleotide counting from the ATG starting site. Transcriptional and translational start sites were manually annotated based on our previous published pgr sequence [( 25 ); Genbank access number EF155644] due to annotation errors in databases.
    Figure Legend Snippet: Genomic DNA sequence of first exon (in upper case) and flanking intron regions (in lower case) of nuclear progestin receptor (nPR or pgr ) . First and second TALEN targeting sites are highlighted either in green or yellow, respectively. Translation start site (ATG) and restriction enzyme recognition sites ( Bss HII: GCGCGC; Sac II, CCGCGG) are indicated by box. Forward and reverse PCR primers for amplification of genomic region including TALEN targeting sites are underlined. The numbers on the far right of the figure indicate the positions of the nucleotide counting from the ATG starting site. Transcriptional and translational start sites were manually annotated based on our previous published pgr sequence [( 25 ); Genbank access number EF155644] due to annotation errors in databases.

    Techniques Used: Sequencing, Polymerase Chain Reaction, Amplification

    7) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    8) Product Images from "Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase"

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Figure Legend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Techniques Used: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.
    Figure Legend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Techniques Used: Sequencing

    9) Product Images from "Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase"

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Figure Legend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Techniques Used: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.
    Figure Legend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Techniques Used: Sequencing

    10) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    11) Product Images from "Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients"

    Article Title: Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients

    Journal: Journal of Oral Microbiology

    doi: 10.3402/jom.v3i0.6362

    Pulsed-field gel electrophoresis (PFGE) patterns of Bss HII restriction endonuclease analysis of genomic DNA (REAG-B) with dendrogram for Candida albicans isolates. A genetic similarity percentage is shown above the dendrogram. Patient identification (Pt ID), sample site (Source), and number of days after admission to the intensive care unit that the strain was isolated (Day) are included along each PFGE lane. Saccharomyces cerevisiae DNA concatemers and λ DNA ladder were used as size markers. Sizes are measured in kilobases (Kb). C. albicans strain SC5314 (ATCC MYA-2876) was used as the control strain. Abbreviations: SG, supragingival dental plaque; TS, tracheal secretion; BL, bronchoalveolar lavage.
    Figure Legend Snippet: Pulsed-field gel electrophoresis (PFGE) patterns of Bss HII restriction endonuclease analysis of genomic DNA (REAG-B) with dendrogram for Candida albicans isolates. A genetic similarity percentage is shown above the dendrogram. Patient identification (Pt ID), sample site (Source), and number of days after admission to the intensive care unit that the strain was isolated (Day) are included along each PFGE lane. Saccharomyces cerevisiae DNA concatemers and λ DNA ladder were used as size markers. Sizes are measured in kilobases (Kb). C. albicans strain SC5314 (ATCC MYA-2876) was used as the control strain. Abbreviations: SG, supragingival dental plaque; TS, tracheal secretion; BL, bronchoalveolar lavage.

    Techniques Used: Pulsed-Field Gel, Electrophoresis, Isolation

    12) Product Images from "Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase"

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Figure Legend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Techniques Used: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.
    Figure Legend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Techniques Used: Sequencing

    13) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    14) Product Images from "Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients"

    Article Title: Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients

    Journal: Journal of Oral Microbiology

    doi: 10.3402/jom.v3i0.6362

    Pulsed-field gel electrophoresis (PFGE) patterns of Bss HII restriction endonuclease analysis of genomic DNA (REAG-B) with dendrogram for Candida albicans isolates. A genetic similarity percentage is shown above the dendrogram. Patient identification (Pt ID), sample site (Source), and number of days after admission to the intensive care unit that the strain was isolated (Day) are included along each PFGE lane. Saccharomyces cerevisiae DNA concatemers and λ DNA ladder were used as size markers. Sizes are measured in kilobases (Kb). C. albicans strain SC5314 (ATCC MYA-2876) was used as the control strain. Abbreviations: SG, supragingival dental plaque; TS, tracheal secretion; BL, bronchoalveolar lavage.
    Figure Legend Snippet: Pulsed-field gel electrophoresis (PFGE) patterns of Bss HII restriction endonuclease analysis of genomic DNA (REAG-B) with dendrogram for Candida albicans isolates. A genetic similarity percentage is shown above the dendrogram. Patient identification (Pt ID), sample site (Source), and number of days after admission to the intensive care unit that the strain was isolated (Day) are included along each PFGE lane. Saccharomyces cerevisiae DNA concatemers and λ DNA ladder were used as size markers. Sizes are measured in kilobases (Kb). C. albicans strain SC5314 (ATCC MYA-2876) was used as the control strain. Abbreviations: SG, supragingival dental plaque; TS, tracheal secretion; BL, bronchoalveolar lavage.

    Techniques Used: Pulsed-Field Gel, Electrophoresis, Isolation

    15) Product Images from "Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes"

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    Journal: Nucleic Acids Research

    doi:

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.
    Figure Legend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Techniques Used: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.
    Figure Legend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.
    Figure Legend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Techniques Used: Hybridization, Marker

    Related Articles

    other:

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: Each of the two 6-bp cutters chosen to elaborate the map, Bss HII and Mlu I, gives an average spacing of 40 kb.

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: In order to place the Bss HII and Mlu I restriction sites on each chromosome, we developed a mapping procedure applied to individual chromosomes (DDIC-PFGE) that can be viewed as the counterpart of 2D-PFGE in bacterial genomics ( ).

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes
    Article Snippet: The latter are digested by Bss HII in 9.5- and 2.5-kb fragments (Fig. B) and the Bss HII fragments that we have placed at extreme positions (44 and 92 kb) yield a 2.5-kb fragment after Mlu I digestion (Fig. A).

    Plasmid Preparation:

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase
    Article Snippet: .. According to the pBluescript map, we would expect to see the DNA fragment next to the pBluescript vector at the T3 promoter site in pBluMH to be about 0.1 kb smaller when double-digested with Bss HII + Pst I than when digested with Bss HII alone. .. The Southern hybridization results show that the 1.1-kb fragment from Bss HII digestion corresponds to a 1-kb fragment in the Bss HII + Pst I double-digestion.

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase
    Article Snippet: The plasmid (pBluMH) from the positive colonies was purified and mapped to locate the position of the probe in the insert. .. The plasmid was digested separately with Eag I and Bss HII, restriction enzymes that each would cut the probe sequence at only one site (Fig. ). .. The plasmid also was double-digested with Eag I + Pst I, and Bss HII + Pst I.

    Sequencing:

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase
    Article Snippet: The plasmid (pBluMH) from the positive colonies was purified and mapped to locate the position of the probe in the insert. .. The plasmid was digested separately with Eag I and Bss HII, restriction enzymes that each would cut the probe sequence at only one site (Fig. ). .. The plasmid also was double-digested with Eag I + Pst I, and Bss HII + Pst I.

    Electrophoresis:

    Article Title: Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages
    Article Snippet: Phage DNA was purified from the phage suspension by phenol extraction, followed by ethanol precipitation. .. A total of 300 ng of phage DNA was digested with the endonuclease HindIII or BssHII as described by the manufacturer (New England BioLabs) and visualized by electrophoresis in a 0.7% agarose gel. ..

    Agarose Gel Electrophoresis:

    Article Title: Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages
    Article Snippet: Phage DNA was purified from the phage suspension by phenol extraction, followed by ethanol precipitation. .. A total of 300 ng of phage DNA was digested with the endonuclease HindIII or BssHII as described by the manufacturer (New England BioLabs) and visualized by electrophoresis in a 0.7% agarose gel. ..

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    New England Biolabs bss hii
    Restriction digestion analysis of RM4511 DNA. (A) Detection of the RM4511 mck mutation by Bbr PI digestion. Autoradiograph of Bbr PI-digested (Roche) virion DNA from parental RM427 + , RM4503, and two isolates of RM4511 (RM4511.1 and RM4511.2) following electrophoretic separation on a 1% agarose gel and hybridization with a Hin dIII/ Afl II fragment mck probe. (B) Detection of the EGFP-puro insert in RM4503 and RM4511. Autoradiograph of electrophoretically separated, <t>Bss</t> <t>HII-digested</t> 32 P-end-labeled DNA fragments. (C) Autoradiograph of electrophoretically separated 32 P-end-labeled Hin dIII fragments of DNA from RM427 + , RM4503, and two isolates of RM4511.
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    Restriction digestion analysis of RM4511 DNA. (A) Detection of the RM4511 mck mutation by Bbr PI digestion. Autoradiograph of Bbr PI-digested (Roche) virion DNA from parental RM427 + , RM4503, and two isolates of RM4511 (RM4511.1 and RM4511.2) following electrophoretic separation on a 1% agarose gel and hybridization with a Hin dIII/ Afl II fragment mck probe. (B) Detection of the EGFP-puro insert in RM4503 and RM4511. Autoradiograph of electrophoretically separated, Bss HII-digested 32 P-end-labeled DNA fragments. (C) Autoradiograph of electrophoretically separated 32 P-end-labeled Hin dIII fragments of DNA from RM427 + , RM4503, and two isolates of RM4511.

    Journal: Journal of Virology

    Article Title: Murine Cytomegalovirus CC Chemokine Homolog MCK-2 (m131-129) Is a Determinant of Dissemination That Increases Inflammation at Initial Sites of Infection

    doi: 10.1128/JVI.75.20.9966-9976.2001

    Figure Lengend Snippet: Restriction digestion analysis of RM4511 DNA. (A) Detection of the RM4511 mck mutation by Bbr PI digestion. Autoradiograph of Bbr PI-digested (Roche) virion DNA from parental RM427 + , RM4503, and two isolates of RM4511 (RM4511.1 and RM4511.2) following electrophoretic separation on a 1% agarose gel and hybridization with a Hin dIII/ Afl II fragment mck probe. (B) Detection of the EGFP-puro insert in RM4503 and RM4511. Autoradiograph of electrophoretically separated, Bss HII-digested 32 P-end-labeled DNA fragments. (C) Autoradiograph of electrophoretically separated 32 P-end-labeled Hin dIII fragments of DNA from RM427 + , RM4503, and two isolates of RM4511.

    Article Snippet: Virion DNA (0.5 to 1.0 μg) was digested with Hin dIII, Bss HII, Afl II, or Spe I (New England Biolabs, Beverly, Mass.) and end labeled in the presence of 2.5 μCi of [α-32 P]dCTP (Amersham), 125 μM each dATP, dGTP, and dTTP, and 0.5 U of Klenow polymerase (Roche, Indianapolis, Ind.) for 15 min at room temperature in 20 μl of restriction enzyme buffer.

    Techniques: Mutagenesis, Autoradiography, Agarose Gel Electrophoresis, Hybridization, Labeling

    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    doi:

    Figure Lengend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Article Snippet: The plasmid was digested separately with Eag I and Bss HII, restriction enzymes that each would cut the probe sequence at only one site (Fig. ).

    Techniques: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    doi:

    Figure Lengend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Article Snippet: The plasmid was digested separately with Eag I and Bss HII, restriction enzymes that each would cut the probe sequence at only one site (Fig. ).

    Techniques: Sequencing

    DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Journal: Nucleic Acids Research

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    doi:

    Figure Lengend Snippet: DDIC-PFGE of the chromosome VII of E.cuniculi . After isolation of the chromosome VII from the molecular karyotype, chromosomal DNA was digested by Bss HII ( A ) or Mlu I ( B ), and radiolabelled. A second PFGE was performed leading to the separation of fragments (sizes in kb, above the autoradiograph). A second digestion was then performed with Mlu I (A) or Bss HII (B), and the fragments separated with a third PFGE, orthogonal to the second (sizes in kb, on the right of each autoradiograph). Autoradiographs on (A) and (B) are therefore called VII B/M DDIC-PFGE and VII M/B DDIC-PFGE, respectively.

    Article Snippet: Each of the two 6-bp cutters chosen to elaborate the map, Bss HII and Mlu I, gives an average spacing of 40 kb.

    Techniques: Isolation, Autoradiography

    KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Journal: Nucleic Acids Research

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    doi:

    Figure Lengend Snippet: KARD-PFGE of the E.cuniculi genome. Autoradiographs of radiolabelled chromosomal DNA are shown after resolution of the molecular karyotype (at the top of the 2D-gel) and in-gel digestion with Bss HII (left part) or Mlu I (right part) followed by a second orthogonal PFGE. Chromosome order is indicated above, the size of standards (in kb) in the middle. The two restriction fragments derived from chromosomes IIIa and IIIb, which can be differentiated by their size, are indicated by arrows. The decreasing intensity of spots in the right part of the lower region of Bss HII KARD autoradiograph is due to a heterogeneous drying of the gel.

    Article Snippet: Each of the two 6-bp cutters chosen to elaborate the map, Bss HII and Mlu I, gives an average spacing of 40 kb.

    Techniques: Two-Dimensional Gel Electrophoresis, Derivative Assay, Autoradiography

    rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Journal: Nucleic Acids Research

    Article Title: Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes

    doi:

    Figure Lengend Snippet: rDNA mapping by hybridisation on KARD-PFGE. Bss HII- and Mlu I-KARD-PFGE dried gels were hybridised with either a 3′ rDNA probe ( A ) or a 16S rDNA probe ( B ) (see Fig. 4A for localisation of the probes). Data confirm the subtelomeric position of the rDNA unit on all the chromosomes and provide information about the Mlu I fragments adjacent to the 12-kb terminal fragments. Marker size is indicated in kb.

    Article Snippet: Each of the two 6-bp cutters chosen to elaborate the map, Bss HII and Mlu I, gives an average spacing of 40 kb.

    Techniques: Hybridization, Marker