bsrgi  (New England Biolabs)


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  • 99
    Name:
    BsrGI
    Description:
    BsrGI 5 000 units
    Catalog Number:
    r0575l
    Price:
    277
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bsrgi
    BsrGI
    BsrGI 5 000 units
    https://www.bioz.com/result/bsrgi/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bsrgi - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The 998-bp aac(3)-IV (Aprr )- and 569-bp ble (Zeor )-containing fragments were PCR amplified from the pFAM1 and pFZE1 plasmids (primer sequences are provided in ), respectively, and purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek). .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above.

    Agarose Gel Electrophoresis:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The 998-bp aac(3)-IV (Aprr )- and 569-bp ble (Zeor )-containing fragments were PCR amplified from the pFAM1 and pFZE1 plasmids (primer sequences are provided in ), respectively, and purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek). .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above.

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. The resulting plasmid backbone (5,374-bp fragment) was purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek, GA, USA) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above. .. The ligated plasmids were transformed into E. coli DH5α, and colonies were selected on LB plus 30 μg/ml Apr (Apr30 ) for pUC18T-mini-Tn 7 T-LAC-Apr transformants or on LB plus 25 μg/ml Zeo (Zeo25 ) for pUC18T-mini-Tn 7 T-LAC-Zeo transformants.

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. The 998-bp aac(3)-IV (Aprr )- and 569-bp ble (Zeor )-containing fragments were PCR amplified from the pFAM1 and pFZE1 plasmids (primer sequences are provided in ), respectively, and purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek).

    Next-Generation Sequencing:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above. .. Further confirmation was carried out by sequencing both plasmids by Illumina Hi-Seq next-generation sequencing (MGH CCIB DNA Core Facility, Cambridge, MA, USA).

    Purification:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The 998-bp aac(3)-IV (Aprr )- and 569-bp ble (Zeor )-containing fragments were PCR amplified from the pFAM1 and pFZE1 plasmids (primer sequences are provided in ), respectively, and purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek). .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above.

    Article Title: Directed evolution of a picomolar-affinity, high-specificity antibody targeting phosphorylated tau
    Article Snippet: Paragraph title: Purification of pThr-231 scFvs ... The pThr-231 scFv gene was inserted into the vector pRS316 (a gift from Dr. Eric Shusta), which had been restriction-digested with NheI and BsrGI (New England Biolabs).

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. The resulting plasmid backbone (5,374-bp fragment) was purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek, GA, USA) according to the manufacturer’s protocol.

    Marker:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above.

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: .. The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. The resulting plasmid backbone (5,374-bp fragment) was purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek, GA, USA) according to the manufacturer’s protocol.

    Sequencing:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above. .. Further confirmation was carried out by sequencing both plasmids by Illumina Hi-Seq next-generation sequencing (MGH CCIB DNA Core Facility, Cambridge, MA, USA).

    Gel Extraction:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The 998-bp aac(3)-IV (Aprr )- and 569-bp ble (Zeor )-containing fragments were PCR amplified from the pFAM1 and pFZE1 plasmids (primer sequences are provided in ), respectively, and purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek). .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above.

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. The resulting plasmid backbone (5,374-bp fragment) was purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek, GA, USA) according to the manufacturer’s protocol.

    Transformation Assay:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above. .. The ligated plasmids were transformed into E. coli DH5α, and colonies were selected on LB plus 30 μg/ml Apr (Apr30 ) for pUC18T-mini-Tn 7 T-LAC-Apr transformants or on LB plus 25 μg/ml Zeo (Zeo25 ) for pUC18T-mini-Tn 7 T-LAC-Zeo transformants.

    Article Title: Directed evolution of a picomolar-affinity, high-specificity antibody targeting phosphorylated tau
    Article Snippet: The pThr-231 scFv gene was inserted into the vector pRS316 (a gift from Dr. Eric Shusta), which had been restriction-digested with NheI and BsrGI (New England Biolabs). .. The pRS316 plasmid was transformed into S. cerevisiae YVH10 cells ( ) (a gift from Dr. Eric Shusta) for protein secretion and purification.

    Plasmid Preparation:

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: .. PCR products were digested with BsrGI and SacII (New England Biolabs) and the fragments ligated into the mini-Tn 7 plasmid backbone digested with the same enzymes mentioned above. .. The ligated plasmids were transformed into E. coli DH5α, and colonies were selected on LB plus 30 μg/ml Apr (Apr30 ) for pUC18T-mini-Tn 7 T-LAC-Apr transformants or on LB plus 25 μg/ml Zeo (Zeo25 ) for pUC18T-mini-Tn 7 T-LAC-Zeo transformants.

    Article Title: Directed evolution of a picomolar-affinity, high-specificity antibody targeting phosphorylated tau
    Article Snippet: .. The pThr-231 scFv gene was inserted into the vector pRS316 (a gift from Dr. Eric Shusta), which had been restriction-digested with NheI and BsrGI (New England Biolabs). .. This plasmid included a C-terminal hexahistidine epitope tag.

    Article Title: Next Generation of Tn7-Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii
    Article Snippet: .. The Gmr marker from pUC18T-mini-Tn 7 T-LAC-Gm was excised using BsrGI and SacII (New England Biolabs, ON, Canada), leaving the FRT sites in the plasmid backbone intact. .. The resulting plasmid backbone (5,374-bp fragment) was purified from the agarose gel using the E.Z.N.A. gel extraction kit (Omega Bio-Tek, GA, USA) according to the manufacturer’s protocol.

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