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    Name:
    BspEI
    Description:
    BspEI 5 000 units
    Catalog Number:
    r0540l
    Price:
    282
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bspei
    BspEI
    BspEI 5 000 units
    https://www.bioz.com/result/bspei/product/New England Biolabs
    Average 95 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    bspei - by Bioz Stars, 2020-08
    95/100 stars

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    1) Product Images from "Induction of proto-oncogene BRF2 in breast cancer cells by the dietary soybean isoflavone daidzein"

    Article Title: Induction of proto-oncogene BRF2 in breast cancer cells by the dietary soybean isoflavone daidzein

    Journal: BMC Cancer

    doi: 10.1186/s12885-015-1914-5

    Daidzein changes DNA methylation at the BRF2 promoter specifically. CpG methylation- sensitive restriction enzymes cut sites are shown. Transcription start site (TSS) is indicated by +1. MethPrimer program was used to predict the location of CpG islands within the BRF2 (402–556 bp) and BRF1 (50–910 bp) promoters, as indicated by black arrow. Black bars denote the binding sites for primers used in the methylation profile analysis. a-d MCF-7 and ( e ) MDA-MB-231 cells were treated with 0, 3, 10 μM daidzein for 48 h. The genomic DNA was harvested and digested with methylation-sensitive restriction enzymes with cut sites within the promoter, noted on BRF2 and BRF1 promoter schematic, and as a negative control one methylation sensitive enzyme with no recognition sites in the promoter was used. BspEI and BsRFI do not have recognition sites in BRF2 and BRF1 promoters, respectively. The digestion profile was then analyzed by qPCR using primers spanning the BRF2 ( a , e, f ) and BRF1 ( b ) promoter regions, U6 ( c ) and tRNA i Met ( d ) genes. DNA methylation levels were calculated using ΔΔCt method with RPS13 expression levels used as a reference for normalization. Data presented are average of three independent experiments. Statistical analysis was performed using one-way ANOVA a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p
    Figure Legend Snippet: Daidzein changes DNA methylation at the BRF2 promoter specifically. CpG methylation- sensitive restriction enzymes cut sites are shown. Transcription start site (TSS) is indicated by +1. MethPrimer program was used to predict the location of CpG islands within the BRF2 (402–556 bp) and BRF1 (50–910 bp) promoters, as indicated by black arrow. Black bars denote the binding sites for primers used in the methylation profile analysis. a-d MCF-7 and ( e ) MDA-MB-231 cells were treated with 0, 3, 10 μM daidzein for 48 h. The genomic DNA was harvested and digested with methylation-sensitive restriction enzymes with cut sites within the promoter, noted on BRF2 and BRF1 promoter schematic, and as a negative control one methylation sensitive enzyme with no recognition sites in the promoter was used. BspEI and BsRFI do not have recognition sites in BRF2 and BRF1 promoters, respectively. The digestion profile was then analyzed by qPCR using primers spanning the BRF2 ( a , e, f ) and BRF1 ( b ) promoter regions, U6 ( c ) and tRNA i Met ( d ) genes. DNA methylation levels were calculated using ΔΔCt method with RPS13 expression levels used as a reference for normalization. Data presented are average of three independent experiments. Statistical analysis was performed using one-way ANOVA a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p

    Techniques Used: DNA Methylation Assay, CpG Methylation Assay, Binding Assay, Methylation, Multiple Displacement Amplification, Negative Control, Real-time Polymerase Chain Reaction, Expressing

    Related Articles

    Mutagenesis:

    Article Title: Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells
    Article Snippet: .. To generate pENTR-CIBN-MP, we removed mCerulean from pENTR-CIBN-mCerulean-MP through restriction with AgeI (NEB) and BspEI (NEB). pENTR-CRY2olig(E490G)-VH H was generated through site-directed mutagenesis as in FastCloning [ ] ( ). .. Then, we recombined aPKC and aPKCΔN into pHGW, Par6 into pHWR, CIBN constructs into pHW, and CRY2 constructs into pHRW through LR Clonase II (Thermo Fisher Scientific, Waltham, MA, USA)-mediated recombination.

    Clone Assay:

    Article Title: Basal Core Promoter and Precore Mutations in the Hepatitis B Virus Genome Enhance Replication Efficacy of Lamivudine-Resistant Mutants
    Article Snippet: .. HBV 1.0 wt was digested with XhoI and BspEI (796 bp) and cloned into the pACYC177 vector (New England Biolabs, Beverly, Mass.). .. The AflIII-BspEI fragment was then exchanged between the wt and the mutated TOPO-TA vector.

    Generated:

    Article Title: Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells
    Article Snippet: .. To generate pENTR-CIBN-MP, we removed mCerulean from pENTR-CIBN-mCerulean-MP through restriction with AgeI (NEB) and BspEI (NEB). pENTR-CRY2olig(E490G)-VH H was generated through site-directed mutagenesis as in FastCloning [ ] ( ). .. Then, we recombined aPKC and aPKCΔN into pHGW, Par6 into pHWR, CIBN constructs into pHW, and CRY2 constructs into pHRW through LR Clonase II (Thermo Fisher Scientific, Waltham, MA, USA)-mediated recombination.

    other:

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
    Article Snippet: Restriction endonucleases: Bam HI, Bsp EI, Nde I, Sca I, Xma I (New England Biolabs Inc., cat. no. R0136S, R0540S, R0111S, R0122S, R0180S, respectively) T4 DNA ligase (Promega, cat. no. M1801) Lysozyme (Aldrich-Sigma Chemical Co. Ltd, cat. no. 62970) DNAse I (Aldrich-Sigma Chemical Co. Ltd, cat. no. D7291-5MG) RNAse A (Aldrich-Sigma Chemical Co. Ltd, cat. no. R6513-10MG) 6× His mAb–HRP conjugate (Clontech, cat. no. 631210)

    Plasmid Preparation:

    Article Title: Basal Core Promoter and Precore Mutations in the Hepatitis B Virus Genome Enhance Replication Efficacy of Lamivudine-Resistant Mutants
    Article Snippet: .. HBV 1.0 wt was digested with XhoI and BspEI (796 bp) and cloned into the pACYC177 vector (New England Biolabs, Beverly, Mass.). .. The AflIII-BspEI fragment was then exchanged between the wt and the mutated TOPO-TA vector.

    Article Title: An unexpected inhibition of antiviral signaling by virus-encoded tumor suppressor p53 in pancreatic cancer cells
    Article Snippet: .. To create the pUC57-eqFP650-TP53-CC/fs, the pUC57-eqFP650-TP53-CC plasmid was digested with BspEI, filled in with T4 DNA polymerase (NEB, MO203S), and re-ligated. .. All pUC57 plasmids containing the eqFP650-fusion genes were double digested with XhoI and NheI restriction enzymes and the inserts were ligated into the XhoI-NheI digested VSV-XN2-ΔM51 plasmid.

    Article Title: Contribution of DNMT1 to Neuropathic Pain Genesis Partially through Epigenetically Repressing Kcna2 in Primary Afferent Neurons
    Article Snippet: .. Fragments bearing full-length CREB were ligated into proviral plasmids (University of North Carolina Vector Core) using the BspEI and NotI restriction sites (New England Biolabs) driven by the cytomegalovirus promoter. .. Packaging of the rAAV5 viral particles encoding the respective plasmids was performed by the University of North Carolina Vector Core.

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    New England Biolabs bspei
    Daidzein changes DNA methylation at the BRF2 promoter specifically. CpG methylation- sensitive restriction enzymes cut sites are shown. Transcription start site (TSS) is indicated by +1. MethPrimer program was used to predict the location of CpG islands within the BRF2 (402–556 bp) and BRF1 (50–910 bp) promoters, as indicated by black arrow. Black bars denote the binding sites for primers used in the methylation profile analysis. a-d MCF-7 and ( e ) MDA-MB-231 cells were treated with 0, 3, 10 μM daidzein for 48 h. The genomic DNA was harvested and digested with methylation-sensitive restriction enzymes with cut sites within the promoter, noted on BRF2 and BRF1 promoter schematic, and as a negative control one methylation sensitive enzyme with no recognition sites in the promoter was used. <t>BspEI</t> and <t>BsRFI</t> do not have recognition sites in BRF2 and BRF1 promoters, respectively. The digestion profile was then analyzed by qPCR using primers spanning the BRF2 ( a , e, f ) and BRF1 ( b ) promoter regions, U6 ( c ) and tRNA i Met ( d ) genes. DNA methylation levels were calculated using ΔΔCt method with RPS13 expression levels used as a reference for normalization. Data presented are average of three independent experiments. Statistical analysis was performed using one-way ANOVA a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p
    Bspei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspei/product/New England Biolabs
    Average 95 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    bspei - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

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    Daidzein changes DNA methylation at the BRF2 promoter specifically. CpG methylation- sensitive restriction enzymes cut sites are shown. Transcription start site (TSS) is indicated by +1. MethPrimer program was used to predict the location of CpG islands within the BRF2 (402–556 bp) and BRF1 (50–910 bp) promoters, as indicated by black arrow. Black bars denote the binding sites for primers used in the methylation profile analysis. a-d MCF-7 and ( e ) MDA-MB-231 cells were treated with 0, 3, 10 μM daidzein for 48 h. The genomic DNA was harvested and digested with methylation-sensitive restriction enzymes with cut sites within the promoter, noted on BRF2 and BRF1 promoter schematic, and as a negative control one methylation sensitive enzyme with no recognition sites in the promoter was used. BspEI and BsRFI do not have recognition sites in BRF2 and BRF1 promoters, respectively. The digestion profile was then analyzed by qPCR using primers spanning the BRF2 ( a , e, f ) and BRF1 ( b ) promoter regions, U6 ( c ) and tRNA i Met ( d ) genes. DNA methylation levels were calculated using ΔΔCt method with RPS13 expression levels used as a reference for normalization. Data presented are average of three independent experiments. Statistical analysis was performed using one-way ANOVA a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p

    Journal: BMC Cancer

    Article Title: Induction of proto-oncogene BRF2 in breast cancer cells by the dietary soybean isoflavone daidzein

    doi: 10.1186/s12885-015-1914-5

    Figure Lengend Snippet: Daidzein changes DNA methylation at the BRF2 promoter specifically. CpG methylation- sensitive restriction enzymes cut sites are shown. Transcription start site (TSS) is indicated by +1. MethPrimer program was used to predict the location of CpG islands within the BRF2 (402–556 bp) and BRF1 (50–910 bp) promoters, as indicated by black arrow. Black bars denote the binding sites for primers used in the methylation profile analysis. a-d MCF-7 and ( e ) MDA-MB-231 cells were treated with 0, 3, 10 μM daidzein for 48 h. The genomic DNA was harvested and digested with methylation-sensitive restriction enzymes with cut sites within the promoter, noted on BRF2 and BRF1 promoter schematic, and as a negative control one methylation sensitive enzyme with no recognition sites in the promoter was used. BspEI and BsRFI do not have recognition sites in BRF2 and BRF1 promoters, respectively. The digestion profile was then analyzed by qPCR using primers spanning the BRF2 ( a , e, f ) and BRF1 ( b ) promoter regions, U6 ( c ) and tRNA i Met ( d ) genes. DNA methylation levels were calculated using ΔΔCt method with RPS13 expression levels used as a reference for normalization. Data presented are average of three independent experiments. Statistical analysis was performed using one-way ANOVA a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p

    Article Snippet: Restriction enzymes AciI, AscI, BanI, BfuAI, BsrFI, BsrBI, BseYI, BfuAI, BspEI, Cac81, FspI, NciI, NruI (New England Biolabs) and HpyCH4III/Taal (Fermentas) were used.

    Techniques: DNA Methylation Assay, CpG Methylation Assay, Binding Assay, Methylation, Multiple Displacement Amplification, Negative Control, Real-time Polymerase Chain Reaction, Expressing

    were subjected to restriction digestion with Xma I and Bsp

    Journal: Nature protocols

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

    doi: 10.1038/nprot.2008.250

    Figure Lengend Snippet: were subjected to restriction digestion with Xma I and Bsp

    Article Snippet: Restriction endonucleases: Bam HI, Bsp EI, Nde I, Sca I, Xma I (New England Biolabs Inc., cat. no. R0136S, R0540S, R0111S, R0122S, R0180S, respectively) T4 DNA ligase (Promega, cat. no. M1801) Lysozyme (Aldrich-Sigma Chemical Co. Ltd, cat. no. 62970) DNAse I (Aldrich-Sigma Chemical Co. Ltd, cat. no. D7291-5MG) RNAse A (Aldrich-Sigma Chemical Co. Ltd, cat. no. R6513-10MG) 6× His mAb–HRP conjugate (Clontech, cat. no. 631210)

    Techniques: