Bsmbi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 99 stars, based on 33 article reviews
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1) Product Images from "Highly Efficient One-Step Scarless Protein Tagging by Type IIS Restriction Endonuclease-Mediated Precision Cloning"
Article Title: Highly Efficient One-Step Scarless Protein Tagging by Type IIS Restriction Endonuclease-Mediated Precision Cloning
Journal: Biochemical and biophysical research communications
Figure Legend Snippet: Procedure to make a template clone ( a ) In the original clone, two unique sites recognized by two different type II restriction enzymes (labeled as TII-e1 and TII-e2) are selected in the gene of interest. The number x amino acid encoded by N 2 N 3 N 4 (in red) is indicated by aa x , or next amino acid by aa x+1 (in green). A template fragment contains a TIIS DNA cassette (highlighted in blue box) in between aa x and aa x+1 . All the cutting positions are indicated by arrows and complementary bases by apostrophe (’). ( b ) pBS-KSII contains a multiple cloning site (MCS) and a BsaI in the ampicillin (Amp) coding gene, which was destroyed by site-directed mutagenesis to create pBS-KSII-4B. Wild-type pBS-KSII was linearized by BsaI and two overlapping fragments were released by BsaI and NaeI double digestion. pBS-KSII-4B does not have BsaI, BbsI, BsmBI, and BfuAI/BspMI and can be linearized by NaeI. Three brighter bands from GeneRuler 1kb plus DNA marker are labeled.
Techniques Used: Labeling, Clone Assay, Mutagenesis, Marker