bse ri  (New England Biolabs)


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    New England Biolabs bse ri
    Electrophoretic pattern of PCR-RFLP of genomic DNA. The lanes are AA: <t>CD4.AA;</t> AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder. The 366 bp-fragment was amplified from genomic DNA using primer pair for exon 3 (See Table 1 ). The PCR product was digested with <t>Bse</t> RI. PCR fragments with genotype of CD4.AA , CD4.AB , and CD4.BB showed single fragment (366 bp), three fragments (366, 260, and 106 bp), and two fragments (260 and 106 bp), respectively
    Bse Ri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bse ri/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bse ri - by Bioz Stars, 2021-04
    86/100 stars

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    1) Product Images from "Identification and characterization of two CD4 alleles in Microminipigs"

    Article Title: Identification and characterization of two CD4 alleles in Microminipigs

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-016-0856-8

    Electrophoretic pattern of PCR-RFLP of genomic DNA. The lanes are AA: CD4.AA; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder. The 366 bp-fragment was amplified from genomic DNA using primer pair for exon 3 (See Table 1 ). The PCR product was digested with Bse RI. PCR fragments with genotype of CD4.AA , CD4.AB , and CD4.BB showed single fragment (366 bp), three fragments (366, 260, and 106 bp), and two fragments (260 and 106 bp), respectively
    Figure Legend Snippet: Electrophoretic pattern of PCR-RFLP of genomic DNA. The lanes are AA: CD4.AA; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder. The 366 bp-fragment was amplified from genomic DNA using primer pair for exon 3 (See Table 1 ). The PCR product was digested with Bse RI. PCR fragments with genotype of CD4.AA , CD4.AB , and CD4.BB showed single fragment (366 bp), three fragments (366, 260, and 106 bp), and two fragments (260 and 106 bp), respectively

    Techniques Used: Polymerase Chain Reaction, Amplification

    Electrophoretic pattern of RT-PCR products after enzyme digestion with Bse RI. The lanes are AA: CD4.AA ; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder . The 400 bp ( a ) and 595 bp ( b ) of the CD4 sequence were amplified from cDNA using primer sets shown in Table 2 and the amplified products were digested with Bse RI. a After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 400 bp-fragment (400 bp), three fragments of 400, 303 and 97 bp, and two fragments of 303 and 97 bp, respectively. b After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 595 bp-fragment, three fragments of 595, 300 and 295 bp, and two fragments of 300 and 295 bp, respectively
    Figure Legend Snippet: Electrophoretic pattern of RT-PCR products after enzyme digestion with Bse RI. The lanes are AA: CD4.AA ; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder . The 400 bp ( a ) and 595 bp ( b ) of the CD4 sequence were amplified from cDNA using primer sets shown in Table 2 and the amplified products were digested with Bse RI. a After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 400 bp-fragment (400 bp), three fragments of 400, 303 and 97 bp, and two fragments of 303 and 97 bp, respectively. b After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 595 bp-fragment, three fragments of 595, 300 and 295 bp, and two fragments of 300 and 295 bp, respectively

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

    2) Product Images from "DNA marker development by the allele-specific detection of powdery mildew resistance loci derived from Japanese domestic tobacco cultivar ‘Kokubu’"

    Article Title: DNA marker development by the allele-specific detection of powdery mildew resistance loci derived from Japanese domestic tobacco cultivar ‘Kokubu’

    Journal: Breeding Science

    doi: 10.1270/jsbbs.20011

    Agarose gel electrophoresis profiles of NtMLO alleles detected using the cleaved amplified polymorphic sequence (CAPS) method. Amplified DNA fragments were digested by Bci T130I or Bfa I for NtMLO1 , and Bse RI for NtMLO2 . Bci T130I and Bfa I digests the wild-type (susceptible) and mutant (resistant) NtMLO1 fragments, respectively, and Bse RI digests mutant (resistant) NtMLO2 fragments. In the heterozygotes, three (digested and undigested) DNA fragments are generated by the restriction enzyme treatment. 1: ‘Tsukuba 1’ (resistant); 2: ‘K326’ (susceptible); 3: F 1 hybrid of ‘Tsukuba 1’ × ‘K326’ (susceptible); 4: ‘Kokubu’ (resistant); 5: ‘Ibusuki’ (susceptible); 6: F 1 hybrid of ‘Kokubu’ × ‘Ibusuki’ (susceptible).
    Figure Legend Snippet: Agarose gel electrophoresis profiles of NtMLO alleles detected using the cleaved amplified polymorphic sequence (CAPS) method. Amplified DNA fragments were digested by Bci T130I or Bfa I for NtMLO1 , and Bse RI for NtMLO2 . Bci T130I and Bfa I digests the wild-type (susceptible) and mutant (resistant) NtMLO1 fragments, respectively, and Bse RI digests mutant (resistant) NtMLO2 fragments. In the heterozygotes, three (digested and undigested) DNA fragments are generated by the restriction enzyme treatment. 1: ‘Tsukuba 1’ (resistant); 2: ‘K326’ (susceptible); 3: F 1 hybrid of ‘Tsukuba 1’ × ‘K326’ (susceptible); 4: ‘Kokubu’ (resistant); 5: ‘Ibusuki’ (susceptible); 6: F 1 hybrid of ‘Kokubu’ × ‘Ibusuki’ (susceptible).

    Techniques Used: Agarose Gel Electrophoresis, Amplification, Sequencing, Mutagenesis, Generated

    Alignment of NtMLO genomic DNA sequences and restriction enzyme recognition sites used in the cleaved amplified polymorphic sequence (CAPS) method. The NtMLO1 gene of powdery mildew-resistant cultivars harbors two-base transitions (AG to TA) at the 3ʹ end of intron 7. In the NtMLO2 gene, the fourth and fifth nucleotide (AG) of intron 6 are deleted in powdery mildew-resistant cultivars. The CAPS method detects these polymorphisms by restriction enzyme digestion patterns. Bci T130I and Bfa I recognize and digest the wild-type (powdery mildew-susceptible) and mutant (powdery mildew-resistant) NtMLO1 genomic DNA sequence, respectively. Bse RI recognizes and digests the mutant (powdery mildew-resistant) NtMLO2 genomic DNA sequence. Intron sequences are shown in bold letters. Arrow heads indicate the digestion site of each enzyme.
    Figure Legend Snippet: Alignment of NtMLO genomic DNA sequences and restriction enzyme recognition sites used in the cleaved amplified polymorphic sequence (CAPS) method. The NtMLO1 gene of powdery mildew-resistant cultivars harbors two-base transitions (AG to TA) at the 3ʹ end of intron 7. In the NtMLO2 gene, the fourth and fifth nucleotide (AG) of intron 6 are deleted in powdery mildew-resistant cultivars. The CAPS method detects these polymorphisms by restriction enzyme digestion patterns. Bci T130I and Bfa I recognize and digest the wild-type (powdery mildew-susceptible) and mutant (powdery mildew-resistant) NtMLO1 genomic DNA sequence, respectively. Bse RI recognizes and digests the mutant (powdery mildew-resistant) NtMLO2 genomic DNA sequence. Intron sequences are shown in bold letters. Arrow heads indicate the digestion site of each enzyme.

    Techniques Used: Amplification, Sequencing, Mutagenesis

    Related Articles

    Plasmid Preparation:

    Article Title: YC-1 BINDING TO THE BETA SUBUNIT OF SOLUBLE GUANYLYL CYCLASE OVERCOMES ALLOSTERIC INHIBITION BY THE ALPHA SUBUNIT
    Article Snippet: A ligation independent cloning (LIC) approach was undertaken as described, using forward primer 5′- agattggtggc atcggcgtggctagcttctgc-3′, and reverse primer 5′- gaggagagtttagac ttaaccatcctgagccctagcc-3′ (LIC overhang residues are underlined). .. The vector was made ready for ligation using the direct digestion method with Bse RI (New England Biolabs). .. A stop codon was introduced at position 405 to yield wild-type construct Ms sGC-P25α, spanning residues 279–404.

    Ligation:

    Article Title: YC-1 BINDING TO THE BETA SUBUNIT OF SOLUBLE GUANYLYL CYCLASE OVERCOMES ALLOSTERIC INHIBITION BY THE ALPHA SUBUNIT
    Article Snippet: A ligation independent cloning (LIC) approach was undertaken as described, using forward primer 5′- agattggtggc atcggcgtggctagcttctgc-3′, and reverse primer 5′- gaggagagtttagac ttaaccatcctgagccctagcc-3′ (LIC overhang residues are underlined). .. The vector was made ready for ligation using the direct digestion method with Bse RI (New England Biolabs). .. A stop codon was introduced at position 405 to yield wild-type construct Ms sGC-P25α, spanning residues 279–404.

    Mutagenesis:

    Article Title: Detection of a Novel Missense Mutations in Atrichia with Papular Lesions
    Article Snippet: The mutation was identified by comparisons of the HR sequences among the AU patients and control subjects, using the ClustalX multiple sequence alignment program (Strasburg, France). .. The mutation was verified in the patient by comparing the Bse RI (New England Biolabs, Beverly, MA, USA) restriction pattern of his HR gene with those of several family members (his parents, maternal and paternal grandparents, an aunt, two uncles, and a brother), the AU patients, and the control individuals. ..

    Polymerase Chain Reaction:

    Article Title: Identification and characterization of two CD4 alleles in Microminipigs
    Article Snippet: CD4 genotyping by PCR-restriction fragment length polymorphism (PCR-RFLP) method The PCR-RFLP technique in association with the restriction enzyme Bse RI was used to identify and differentiate between the two CD4 alleles. .. PCR amplification was performed on genomic DNA to amplify CD4 exon 3, and the PCR products were digested with an enzyme, Bse RI (New England Biolabs Inc., Ipswich, MA). .. The allele-specific bands were analyzed by 2 % agarose gel electrophoresis.

    Article Title: A single-base substitution within an intronic repetitive element causes dominant retinitis pigmentosa with reduced penetrance
    Article Snippet: PCR products were first assessed to be unique products on a 2% agarose gel, and then digested using two restriction enzymes. .. Bss SI (New England Biolabs, Ipswich, MA), which cuts all PCR products regardless of their allelic origin, was used to reduce the size in order to increase resolution for quantification; Bse RI (New England Biolabs), which specifically recognizes the G allele of SNP rs1058572:G > A and cuts 14 bp away from it (towards the internal part of the PCR product), was used to distinguish PCR products derived from each allele. .. Digested products were run again on a 2% agarose gel and quantified by the ImageJ software.

    Article Title: PCR and Restriction Endonuclease Analysis for Rapid Identification of Human Adenovirus Subgenera
    Article Snippet: The PCR products were analyzed with 8% polyacrylamide gels. .. The 140-bp PCR products generated from clinical isolates were digested with the REs Taq I, Avi II, and Aat II (all from Roche Diagnostics Ltd., Lewes, United Kingdom) and Bse RI and Mnl I (both from New England BioLabs Incorporated, Hitchin, United Kingdom). .. In a total volume of 20 μl, all reaction mixtures were prepared as recommended by the manufacturers, and those with REs Taq I, Avi II, and Aat II were incubated for 3 h at the appropriate temperature and those with REs Mnl I and Bse RI were incubated overnight at the appropriate temperature.

    Amplification:

    Article Title: Identification and characterization of two CD4 alleles in Microminipigs
    Article Snippet: CD4 genotyping by PCR-restriction fragment length polymorphism (PCR-RFLP) method The PCR-RFLP technique in association with the restriction enzyme Bse RI was used to identify and differentiate between the two CD4 alleles. .. PCR amplification was performed on genomic DNA to amplify CD4 exon 3, and the PCR products were digested with an enzyme, Bse RI (New England Biolabs Inc., Ipswich, MA). .. The allele-specific bands were analyzed by 2 % agarose gel electrophoresis.

    Derivative Assay:

    Article Title: A single-base substitution within an intronic repetitive element causes dominant retinitis pigmentosa with reduced penetrance
    Article Snippet: PCR products were first assessed to be unique products on a 2% agarose gel, and then digested using two restriction enzymes. .. Bss SI (New England Biolabs, Ipswich, MA), which cuts all PCR products regardless of their allelic origin, was used to reduce the size in order to increase resolution for quantification; Bse RI (New England Biolabs), which specifically recognizes the G allele of SNP rs1058572:G > A and cuts 14 bp away from it (towards the internal part of the PCR product), was used to distinguish PCR products derived from each allele. .. Digested products were run again on a 2% agarose gel and quantified by the ImageJ software.

    Generated:

    Article Title: PCR and Restriction Endonuclease Analysis for Rapid Identification of Human Adenovirus Subgenera
    Article Snippet: The PCR products were analyzed with 8% polyacrylamide gels. .. The 140-bp PCR products generated from clinical isolates were digested with the REs Taq I, Avi II, and Aat II (all from Roche Diagnostics Ltd., Lewes, United Kingdom) and Bse RI and Mnl I (both from New England BioLabs Incorporated, Hitchin, United Kingdom). .. In a total volume of 20 μl, all reaction mixtures were prepared as recommended by the manufacturers, and those with REs Taq I, Avi II, and Aat II were incubated for 3 h at the appropriate temperature and those with REs Mnl I and Bse RI were incubated overnight at the appropriate temperature.

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    New England Biolabs bse ri
    Agarose gel electrophoresis profiles of NtMLO alleles detected using the cleaved amplified polymorphic sequence (CAPS) method. Amplified DNA fragments were digested by <t>Bci</t> T130I or Bfa I for NtMLO1 , and <t>Bse</t> RI for NtMLO2 . Bci T130I and Bfa I digests the wild-type (susceptible) and mutant (resistant) NtMLO1 fragments, respectively, and Bse RI digests mutant (resistant) NtMLO2 fragments. In the heterozygotes, three (digested and undigested) DNA fragments are generated by the restriction enzyme treatment. 1: ‘Tsukuba 1’ (resistant); 2: ‘K326’ (susceptible); 3: F 1 hybrid of ‘Tsukuba 1’ × ‘K326’ (susceptible); 4: ‘Kokubu’ (resistant); 5: ‘Ibusuki’ (susceptible); 6: F 1 hybrid of ‘Kokubu’ × ‘Ibusuki’ (susceptible).
    Bse Ri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bse ri/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bse ri - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Agarose gel electrophoresis profiles of NtMLO alleles detected using the cleaved amplified polymorphic sequence (CAPS) method. Amplified DNA fragments were digested by Bci T130I or Bfa I for NtMLO1 , and Bse RI for NtMLO2 . Bci T130I and Bfa I digests the wild-type (susceptible) and mutant (resistant) NtMLO1 fragments, respectively, and Bse RI digests mutant (resistant) NtMLO2 fragments. In the heterozygotes, three (digested and undigested) DNA fragments are generated by the restriction enzyme treatment. 1: ‘Tsukuba 1’ (resistant); 2: ‘K326’ (susceptible); 3: F 1 hybrid of ‘Tsukuba 1’ × ‘K326’ (susceptible); 4: ‘Kokubu’ (resistant); 5: ‘Ibusuki’ (susceptible); 6: F 1 hybrid of ‘Kokubu’ × ‘Ibusuki’ (susceptible).

    Journal: Breeding Science

    Article Title: DNA marker development by the allele-specific detection of powdery mildew resistance loci derived from Japanese domestic tobacco cultivar ‘Kokubu’

    doi: 10.1270/jsbbs.20011

    Figure Lengend Snippet: Agarose gel electrophoresis profiles of NtMLO alleles detected using the cleaved amplified polymorphic sequence (CAPS) method. Amplified DNA fragments were digested by Bci T130I or Bfa I for NtMLO1 , and Bse RI for NtMLO2 . Bci T130I and Bfa I digests the wild-type (susceptible) and mutant (resistant) NtMLO1 fragments, respectively, and Bse RI digests mutant (resistant) NtMLO2 fragments. In the heterozygotes, three (digested and undigested) DNA fragments are generated by the restriction enzyme treatment. 1: ‘Tsukuba 1’ (resistant); 2: ‘K326’ (susceptible); 3: F 1 hybrid of ‘Tsukuba 1’ × ‘K326’ (susceptible); 4: ‘Kokubu’ (resistant); 5: ‘Ibusuki’ (susceptible); 6: F 1 hybrid of ‘Kokubu’ × ‘Ibusuki’ (susceptible).

    Article Snippet: The following enzymes were selected for the digestion of the DNA: Bfa I (New England Biolabs) or Bci T130I (Takara) for NtMLO1 fragment digestion; Bse RI (New England Biolabs) for NtMLO2 fragment digestion.

    Techniques: Agarose Gel Electrophoresis, Amplification, Sequencing, Mutagenesis, Generated

    Alignment of NtMLO genomic DNA sequences and restriction enzyme recognition sites used in the cleaved amplified polymorphic sequence (CAPS) method. The NtMLO1 gene of powdery mildew-resistant cultivars harbors two-base transitions (AG to TA) at the 3ʹ end of intron 7. In the NtMLO2 gene, the fourth and fifth nucleotide (AG) of intron 6 are deleted in powdery mildew-resistant cultivars. The CAPS method detects these polymorphisms by restriction enzyme digestion patterns. Bci T130I and Bfa I recognize and digest the wild-type (powdery mildew-susceptible) and mutant (powdery mildew-resistant) NtMLO1 genomic DNA sequence, respectively. Bse RI recognizes and digests the mutant (powdery mildew-resistant) NtMLO2 genomic DNA sequence. Intron sequences are shown in bold letters. Arrow heads indicate the digestion site of each enzyme.

    Journal: Breeding Science

    Article Title: DNA marker development by the allele-specific detection of powdery mildew resistance loci derived from Japanese domestic tobacco cultivar ‘Kokubu’

    doi: 10.1270/jsbbs.20011

    Figure Lengend Snippet: Alignment of NtMLO genomic DNA sequences and restriction enzyme recognition sites used in the cleaved amplified polymorphic sequence (CAPS) method. The NtMLO1 gene of powdery mildew-resistant cultivars harbors two-base transitions (AG to TA) at the 3ʹ end of intron 7. In the NtMLO2 gene, the fourth and fifth nucleotide (AG) of intron 6 are deleted in powdery mildew-resistant cultivars. The CAPS method detects these polymorphisms by restriction enzyme digestion patterns. Bci T130I and Bfa I recognize and digest the wild-type (powdery mildew-susceptible) and mutant (powdery mildew-resistant) NtMLO1 genomic DNA sequence, respectively. Bse RI recognizes and digests the mutant (powdery mildew-resistant) NtMLO2 genomic DNA sequence. Intron sequences are shown in bold letters. Arrow heads indicate the digestion site of each enzyme.

    Article Snippet: The following enzymes were selected for the digestion of the DNA: Bfa I (New England Biolabs) or Bci T130I (Takara) for NtMLO1 fragment digestion; Bse RI (New England Biolabs) for NtMLO2 fragment digestion.

    Techniques: Amplification, Sequencing, Mutagenesis

    Electrophoretic pattern of PCR-RFLP of genomic DNA. The lanes are AA: CD4.AA; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder. The 366 bp-fragment was amplified from genomic DNA using primer pair for exon 3 (See Table 1 ). The PCR product was digested with Bse RI. PCR fragments with genotype of CD4.AA , CD4.AB , and CD4.BB showed single fragment (366 bp), three fragments (366, 260, and 106 bp), and two fragments (260 and 106 bp), respectively

    Journal: BMC Veterinary Research

    Article Title: Identification and characterization of two CD4 alleles in Microminipigs

    doi: 10.1186/s12917-016-0856-8

    Figure Lengend Snippet: Electrophoretic pattern of PCR-RFLP of genomic DNA. The lanes are AA: CD4.AA; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder. The 366 bp-fragment was amplified from genomic DNA using primer pair for exon 3 (See Table 1 ). The PCR product was digested with Bse RI. PCR fragments with genotype of CD4.AA , CD4.AB , and CD4.BB showed single fragment (366 bp), three fragments (366, 260, and 106 bp), and two fragments (260 and 106 bp), respectively

    Article Snippet: PCR amplification was performed on genomic DNA to amplify CD4 exon 3, and the PCR products were digested with an enzyme, Bse RI (New England Biolabs Inc., Ipswich, MA).

    Techniques: Polymerase Chain Reaction, Amplification

    Electrophoretic pattern of RT-PCR products after enzyme digestion with Bse RI. The lanes are AA: CD4.AA ; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder . The 400 bp ( a ) and 595 bp ( b ) of the CD4 sequence were amplified from cDNA using primer sets shown in Table 2 and the amplified products were digested with Bse RI. a After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 400 bp-fragment (400 bp), three fragments of 400, 303 and 97 bp, and two fragments of 303 and 97 bp, respectively. b After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 595 bp-fragment, three fragments of 595, 300 and 295 bp, and two fragments of 300 and 295 bp, respectively

    Journal: BMC Veterinary Research

    Article Title: Identification and characterization of two CD4 alleles in Microminipigs

    doi: 10.1186/s12917-016-0856-8

    Figure Lengend Snippet: Electrophoretic pattern of RT-PCR products after enzyme digestion with Bse RI. The lanes are AA: CD4.AA ; AB: CD4.AB ; BB: CD4.BB; and the 100 bp ladder . The 400 bp ( a ) and 595 bp ( b ) of the CD4 sequence were amplified from cDNA using primer sets shown in Table 2 and the amplified products were digested with Bse RI. a After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 400 bp-fragment (400 bp), three fragments of 400, 303 and 97 bp, and two fragments of 303 and 97 bp, respectively. b After digestion with Bse RI, CD4.AA , CD4.AB , and CD4.BB showed a 595 bp-fragment, three fragments of 595, 300 and 295 bp, and two fragments of 300 and 295 bp, respectively

    Article Snippet: PCR amplification was performed on genomic DNA to amplify CD4 exon 3, and the PCR products were digested with an enzyme, Bse RI (New England Biolabs Inc., Ipswich, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification