Bsa, supplied by Nacalai, used in various techniques. Bioz Stars score: 88/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 88 stars, based on 40 article reviews
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1) Product Images from "Virucidal and Immunostimulating Activities of Monogalactosyl Diacylglyceride from Coccomyxa sp. KJ, a Green Microalga, against Murine Norovirus and Feline Calicivirus"
Article Title: Virucidal and Immunostimulating Activities of Monogalactosyl Diacylglyceride from Coccomyxa sp. KJ, a Green Microalga, against Murine Norovirus and Feline Calicivirus
Journal: Marine Drugs
Figure Legend Snippet: Virucidal activities of MGDG and sodium hypochlorite (NaClO) against FCV in the presence of bovine serum albumin (BSA). FCV (2 × 10 5 PFU/mL) was mixed with an equal volume of 0 and 100 μg/mL MGDG or NaClO in the presence of 0%, 2%, and 5% BSA and incubated for the indicated time at 37 °C. Results are expressed as the percentages of residual infectivity of MGDG-treated virus compared to the residual infectivity of the mock-treated virus control. Data are the means from independent duplicate assays.
Techniques Used: Incubation, Infection
2) Product Images from "Significant role of host sialylated glycans in the infection and spread of severe acute respiratory syndrome coronavirus 2"
Article Title: Significant role of host sialylated glycans in the infection and spread of severe acute respiratory syndrome coronavirus 2
Journal: PLoS Pathogens
Figure Legend Snippet: α2-6-linked sialosides interacted with S1 subunit of SARS-CoV-2 spike protein. (A) The attachment of compound-pretreated virus to cells. SARS-CoV-2 particles were pretreated with the compounds at 37°C for 60 min, then the free compounds were removed by ultrafiltration. The prepared viruses were treated to VeroE6/TMPRSS2 cells in the absence of compounds at 4°C for 30 min to examine virus-cell attachment. (B) The viral attachment to compound-pretreated cells. The indicated compounds were treated to VeroE6/TMPRSS2 cells at 37°C for 30 min and were then washed out extensively. The prepared cells were incubated with SARS-CoV-2 in the absence of compounds at 4°C for 30 min to examine virus-cell attachment. (A, B) Anti-ACE2 antibody, 100 μg/ml; heparin, 10 U/ml; α2-6SLN-lipo-PGA, 10 μM. (C) Schematic representation of glycan array. Glycan tip immobilized with sialylglycopeptides on a slide glass was incubated with recombinant SARS-CoV-2 S1 at room temperature for 1 h, and then the fluorescence signal was detected. Anti-SARS-CoV-2 S1 antibody was used as primary antibody, and Cy3-conjugated anti-rabbit IgG was used as secondary antibody. (D) The fluorescent response of the glycan array due to the interaction between recombinant SARS-CoV-2 S1 (aa 16–685, Sino Biological) and indicated protein or glycans. The amount of glycan on the neoglycoproteins were 10.8 mol (α2-6-SGP), 10.5 mol (α2-3-SGP) and 10.8 mol (asialo-SGP) of glycan per 1 mol of BSA. The dashed line indicates the nonspecific response level derived from negative control asialo-SGP. Asialo-SGP, 0.7 mg/ml; α2-3-SGP, 0.1 and 1 mg/ml; α2-6-SGP, 0.1 and 1 mg/ml; recombinant ACE2, 0.1 mg/ml.
Techniques Used: Cell Attachment Assay, Incubation, Recombinant, Fluorescence, Derivative Assay, Negative Control
3) Product Images from "Universal glass-forming behavior of in vitro and living cytoplasm"
Article Title: Universal glass-forming behavior of in vitro and living cytoplasm
Journal: Scientific Reports
Figure Legend Snippet: Diffusion in living cytoplasm and in vitro cytoplasm measured by FRAP. ( a ) Fluorescent images of GFP-labeled living spheroplasts of E. coli before, immediately after ( t = 0 s), and 10 s after photobleaching. The photobleached region in a metabolically inactive spheroplast remained dark even at 10 s after photobleaching (lower panels), whereas the dark region was obscured already at t = 0 in normal spheroplasts (upper panels) because of rapid transport. ( b ) Inverse of diffusion coefficients D of GFP in IVCEs/liposomes (open triangles), IVCEs/emulsions (filled triangles) and BSA/liposomes (circles) measured with FRAP. These data were normalized to the value in water as D w / D . The broken and solid curves indicate η / η w in IVCEs and BSA solutions which are measured by PMR (same lines with that in Fig. 1 ), respectively. D w / D of GFP in normal (white bar) and metabolically inactive spheroplast cells (black bar) are also shown.
Techniques Used: Diffusion-based Assay, In Vitro, Labeling, Metabolic Labelling
4) Product Images from "Reliable and sensitive detection of glycosaminoglycan chains with immunoblots"
Article Title: Reliable and sensitive detection of glycosaminoglycan chains with immunoblots
Figure Legend Snippet: Optimization of Immunoblot with CS-56 with a rapid method. ( A ) Amount of antigen required for CS-56 immunoblot. Different amounts of brain lysates obtained from 7-week-old mice prepared in SDS were separated and transferred to a PVDF membrane, followed by blocking with 10% skim milk in PBS-T for 22 h. CS-56 was diluted (1:5000) with 10% CGS-1 containing skim milk solution and incubated with PVDF membrane for 30 min with CDR method. ( B ) Incubation time required for CS-56 immunoblot. Brain lysates (10 μg/lane) prepared in SDS were separated and transferred to PVDF membrane, followed by blocking with 10% skim milk in PBS-T for 22 h at 4°C with agitation. (1) Traditional incubation (2 h) and CDR incubation for (2) 2 h, (3) 30 min and (4) 10 min were performed with CS-56 (1:5000 dilution with 10% CGS-1 containing skim milk solution). The membranes were imaged as a single image and dotted lines indicate the border of individual membranes. L, brain lysates; M, protein marker. ( C ) Effect of diluent for CS-56. Brain lysates (10 μg/lane) prepared in SDS were subjected to CS-56 immunoblot with CDR method. CS-56 was diluted (1:5000) in (1) 2.8 mL of 10% GCS-1 containing 0.2 mL of 5% skim milk in PBS-T, (2) 2.8 mL of 10% GCS-1 containing 0.2 mL of 5% gelatin from cold water fish in PBS-T, (3) 3 mL of 10% CGS-1 and (4) 3 mL of 100% CGS-1. The membranes were imaged as a single image and dotted lines indicate the border of individual membranes. L, brain lysates; M, protein marker. ( D ) Effect of blocking solution. Brain lysates (10 μg/lane) prepared in SDS were separated and transferred to a PVDF membrane. Membranes were blocked for 22 h at 4°C with agitation with (1) 10% skim milk in PBS-T, (2) 5% BSA in PBS-T, (3) PVDF Blocking Reagent for Can Get Signal, and (4) 5% gelatin from cold water fish in PBS-T. Immunoblot was performed as A. The membranes were imaged as a single image and dotted lines indicate the border of individual membranes. L, brain lysates; M, protein marker.
Techniques Used: Mouse Assay, Blocking Assay, Incubation, Marker, Fluorescence In Situ Hybridization
5) Product Images from "The Increased Expression of Integrin ?6 (ITGA6) Enhances Drug Resistance in EVI1high Leukemia"
Article Title: The Increased Expression of Integrin ?6 (ITGA6) Enhances Drug Resistance in EVI1high Leukemia
Journal: PLoS ONE
Figure Legend Snippet: Higher cell adhesion ability in AML cell lines with EVI1 high expression. A . The expression of four integrin genes (ITGB1, ITGB4, ITGA4 and ITGA6), EVI1 and b-actin as a control was determined by semiquantitative RT-PCR in three different EVI1 low and EVI1 high AML cell lines and two primary AML cells lines with high EVI1 expression (PT9 and PT11). B . Six AML cell lines with low or high EVI1expression, as indicated in the figure, were incubated in culture medium on BSA, collagen, fibronectin, laminin or matrigel-coated plates; the percentage of the total number of incubated cells that adhered to the plates was designated as the binding activity (%). Each experiment was performed in triplicate, and the experiments were independently repeated at least three times. The data are given the as the mean ± standard error (S.E). The statistical analysis was performed using the Student's t-test (* p
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation, Binding Assay, Activity Assay