bsa pbs  (Thermo Fisher)


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    Name:
    Bovine Serum Albumin
    Description:
    Bovine Serum Albumin is suitable for use as an enzyme stabilizer during purification or for dilution of restriction endonucleases and nucleic acid modifying enzymes BSA is also commonly used in DNA and protein labeling experiments as a blocking agent to minimize background This BSA is acetylated to inactivate contaminating nucleases and proteases Performance and quality testingPurity is determined by SDS PAGE No detectable contaminating activity is observed in 3 and 5 exonuclease DNA nicking and restriction endonuclease inhibition assays
    Catalog Number:
    15561020
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    ChIP-on-Chip|Cloning|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology|Restriction Enzyme Cloning
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    Structured Review

    Thermo Fisher bsa pbs
    Bovine Serum Albumin is suitable for use as an enzyme stabilizer during purification or for dilution of restriction endonucleases and nucleic acid modifying enzymes BSA is also commonly used in DNA and protein labeling experiments as a blocking agent to minimize background This BSA is acetylated to inactivate contaminating nucleases and proteases Performance and quality testingPurity is determined by SDS PAGE No detectable contaminating activity is observed in 3 and 5 exonuclease DNA nicking and restriction endonuclease inhibition assays
    https://www.bioz.com/result/bsa pbs/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa pbs - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Permeability:

    Article Title: Disruption of stromal hedgehog signaling initiates RNF5-mediated proteasomal degradation of PTEN and accelerates pancreatic tumor growth
    Article Snippet: All devices were cultured for 48 h before measurements by placing approximately 400 μl of medium at the ports and device surface, with the medium being renewed every 24 h. .. Microfluidic hydraulic permeability measurements To measure hydraulic permeability, a rhodamine-bovine serum albumin (rhodamine-BSA) (Molecular Probes) was flowed through the microfluidic device. .. Flow was established by applying a fluidic height difference (2–2.4 cm) between the ports using cell culture medium, measured for each sample.

    Activity Assay:

    Article Title: Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells
    Article Snippet: Quantitative data of migration was analyzed using ImageJ software (NIH, Bethesda, MD, USA). .. Measurement of Proteolytic Enzyme Activity Proteolytic enzyme activity of hASCs during differentiation was measured using the DQ-BSA assay (dye quenched-bovine serum albumin, Molecular Probes, Eugene, OR, USA). .. Fluorescence was appeared by proteolytic cleavage of DQ-BSA.

    other:

    Article Title: Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy
    Article Snippet: Rhodamine-labeled dextran (Rh-dextran; 70kD), succinimidyl esters of Alexa488, Alexa546 and Alexa647 as well as C6-nitrobenzoxadiazole (NBD)-Ceramide (C6-NBD-Cer) and Alexa488-tagged bovine serum albumin (Alexa488-BSA) were purchased from Invitrogen/Molecular Probes (Inc. USA).

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  • 99
    Thermo Fisher imject bsa in pbs
    Immunofluorescence staining of A. fumigatus . Germlings (A) and hyphae (B) were blocked in <t>PBS–1%</t> (wt/vol) <t>BSA</t> and then incubated with the primary anti-Myc antibody. The surface Myc-tagged Hsp70s were indirectly detected with an AF568-conjugated secondary antibody. Scale bar = 10 μm. WT, wild type.
    Imject Bsa In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imject bsa in pbs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    imject bsa in pbs - by Bioz Stars, 2021-03
    99/100 stars
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    99
    Thermo Fisher bsa pbs
    Immunolabeling of TRPV1 in the rat cochlea and dorsal root ganglion (DRG). A : TRPV1 immunolabeling was present in the soma of outer hair cells (OHCs), and inner pillar cells (IPC). B : TRPV1 expression was also identified in the soma of outer pillar cells (OPC), and in C , in Hensen’s cells (HC) and IHCs. D : spiral ganglion neurons (SGN) also expressed TRPV1 labeling. E : immunocytochemical control: negligible labeling was observed in the rat organ of Corti when the TRPV1 primary antibody was replaced by <t>BSA-PBS.</t> F : phalloidin labeling (red) of the organ of Corti shown in E , showing the actiniferous cuticular plate of OHCs (CP, arrow). G : Immunocytochemical control: negligible labeling in the rat organ of Corti was observed when blocking peptide was added to TRPV1 primary antibody incubation media. H : phalloidin labeling of the organ of Corti shown in G . The bar in H represents 50 µm and applies to A–H. I : specific TRPV1 immunolabeling occurs in the cell body of small-diameter DRG neurons. J : immunocytochemical control: negligible labeling in the DRG was observed when the TRPV1 primary antibody was replaced by BSA-PBS. K : immunocytochemical control: negligible labeling in the DRG was observed when blocking peptide was added to TRPV1 primary antibody incubation media. The bar in K represents 100 µm and applies to I–K .
    Bsa Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa pbs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa pbs - by Bioz Stars, 2021-03
    99/100 stars
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    Image Search Results


    Immunofluorescence staining of A. fumigatus . Germlings (A) and hyphae (B) were blocked in PBS–1% (wt/vol) BSA and then incubated with the primary anti-Myc antibody. The surface Myc-tagged Hsp70s were indirectly detected with an AF568-conjugated secondary antibody. Scale bar = 10 μm. WT, wild type.

    Journal: mSphere

    Article Title: Biotinylated Surfome Profiling Identifies Potential Biomarkers for Diagnosis and Therapy of Aspergillus fumigatus Infection

    doi: 10.1128/mSphere.00535-20

    Figure Lengend Snippet: Immunofluorescence staining of A. fumigatus . Germlings (A) and hyphae (B) were blocked in PBS–1% (wt/vol) BSA and then incubated with the primary anti-Myc antibody. The surface Myc-tagged Hsp70s were indirectly detected with an AF568-conjugated secondary antibody. Scale bar = 10 μm. WT, wild type.

    Article Snippet: The fungal materials were washed three times with PBS (pH 7.4) and then blocked with 1% (wt/vol) bovine serum albumin (BSA)–PBS for 1 h at room temperature.

    Techniques: Immunofluorescence, Staining, Incubation

    Confocal images of TRPV1 (A–D) and SP immunolabeling (E–H) expression in (A, E) BA, (B, F) AICA, (C, G) SMA, and (D, H) RA. Arrows indicate labeled: longitudinal (a), circumferential (b) fibers. Insets in A–D, show negligible labeling in BA, AICA, SMA, and RA incubated with antigen-adsorbed TRPV1 antibodies and secondary antibodies. Insets in E–H show negligible labeling in the BA, AICA, SMA, and radiating arterioles incubated with 1% BSA-PBS and secondary antibodies. Scale bars in microns.

    Journal: Neuroscience

    Article Title: CO-LOCALIZATION OF THE VANILLOID CAPSAICIN RECEPTOR AND SUBSTANCE P IN SENSORY NERVE FIBERS INNERVATING COCHLEAR AND VERTEBRO-BASILAR ARTERIES

    doi: 10.1016/j.neuroscience.2003.12.030

    Figure Lengend Snippet: Confocal images of TRPV1 (A–D) and SP immunolabeling (E–H) expression in (A, E) BA, (B, F) AICA, (C, G) SMA, and (D, H) RA. Arrows indicate labeled: longitudinal (a), circumferential (b) fibers. Insets in A–D, show negligible labeling in BA, AICA, SMA, and RA incubated with antigen-adsorbed TRPV1 antibodies and secondary antibodies. Insets in E–H show negligible labeling in the BA, AICA, SMA, and radiating arterioles incubated with 1% BSA-PBS and secondary antibodies. Scale bars in microns.

    Article Snippet: The tissues were washed in fresh 0.02 M PBS (pH 7.4), permeabilized in 0.5% Triton-X 100 (Sigma, St. Louis, MO, USA) in 0.02 M PBS (pH 7.3) for 30 min, and immunoblocked in 10% goat serum in 1% bovine serum albumin (BSA) in 0.02 M PBS (1% BSA-PBS) for 60 min. For immunocytochemistry, tissues were incubated with rabbit anti-TRPV1 antiserum (diluted 1:1000 in 1% BSA-PBS; gift from Dr. Michael J Caterina, John Hopkins University) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-488-conjugated goat anti-rabbit IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS).

    Techniques: Immunolabeling, Expressing, Labeling, Incubation

    Confocal images of the DRG double-immunolabeled for TRPV1 (A), and for SP (B). (C) A subset of neuronal cell bodies co-express TRPV1 and SP (white, arrows) in the merged image. Inset in (A) shows negligible labeling in the DRG when incubated with antigen-adsorbed TRPV1 antibodies and secondary antibodies. Inset in B shows negligible labeling in the DRG when incubated with 1% BSA-PBS and secondary antibodies. Scale bar in microns.

    Journal: Neuroscience

    Article Title: CO-LOCALIZATION OF THE VANILLOID CAPSAICIN RECEPTOR AND SUBSTANCE P IN SENSORY NERVE FIBERS INNERVATING COCHLEAR AND VERTEBRO-BASILAR ARTERIES

    doi: 10.1016/j.neuroscience.2003.12.030

    Figure Lengend Snippet: Confocal images of the DRG double-immunolabeled for TRPV1 (A), and for SP (B). (C) A subset of neuronal cell bodies co-express TRPV1 and SP (white, arrows) in the merged image. Inset in (A) shows negligible labeling in the DRG when incubated with antigen-adsorbed TRPV1 antibodies and secondary antibodies. Inset in B shows negligible labeling in the DRG when incubated with 1% BSA-PBS and secondary antibodies. Scale bar in microns.

    Article Snippet: The tissues were washed in fresh 0.02 M PBS (pH 7.4), permeabilized in 0.5% Triton-X 100 (Sigma, St. Louis, MO, USA) in 0.02 M PBS (pH 7.3) for 30 min, and immunoblocked in 10% goat serum in 1% bovine serum albumin (BSA) in 0.02 M PBS (1% BSA-PBS) for 60 min. For immunocytochemistry, tissues were incubated with rabbit anti-TRPV1 antiserum (diluted 1:1000 in 1% BSA-PBS; gift from Dr. Michael J Caterina, John Hopkins University) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-488-conjugated goat anti-rabbit IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS).

    Techniques: Immunolabeling, Labeling, Incubation

    Immunolabeling of TRPV1 in the rat cochlea and dorsal root ganglion (DRG). A : TRPV1 immunolabeling was present in the soma of outer hair cells (OHCs), and inner pillar cells (IPC). B : TRPV1 expression was also identified in the soma of outer pillar cells (OPC), and in C , in Hensen’s cells (HC) and IHCs. D : spiral ganglion neurons (SGN) also expressed TRPV1 labeling. E : immunocytochemical control: negligible labeling was observed in the rat organ of Corti when the TRPV1 primary antibody was replaced by BSA-PBS. F : phalloidin labeling (red) of the organ of Corti shown in E , showing the actiniferous cuticular plate of OHCs (CP, arrow). G : Immunocytochemical control: negligible labeling in the rat organ of Corti was observed when blocking peptide was added to TRPV1 primary antibody incubation media. H : phalloidin labeling of the organ of Corti shown in G . The bar in H represents 50 µm and applies to A–H. I : specific TRPV1 immunolabeling occurs in the cell body of small-diameter DRG neurons. J : immunocytochemical control: negligible labeling in the DRG was observed when the TRPV1 primary antibody was replaced by BSA-PBS. K : immunocytochemical control: negligible labeling in the DRG was observed when blocking peptide was added to TRPV1 primary antibody incubation media. The bar in K represents 100 µm and applies to I–K .

    Journal: Journal of neurophysiology

    Article Title: Vanilloid Receptors in Hearing: Altered Cochlear Sensitivity by Vanilloids and Expression of TRPV1 in the Organ of Corti

    doi: 10.1152/jn.00919.2002

    Figure Lengend Snippet: Immunolabeling of TRPV1 in the rat cochlea and dorsal root ganglion (DRG). A : TRPV1 immunolabeling was present in the soma of outer hair cells (OHCs), and inner pillar cells (IPC). B : TRPV1 expression was also identified in the soma of outer pillar cells (OPC), and in C , in Hensen’s cells (HC) and IHCs. D : spiral ganglion neurons (SGN) also expressed TRPV1 labeling. E : immunocytochemical control: negligible labeling was observed in the rat organ of Corti when the TRPV1 primary antibody was replaced by BSA-PBS. F : phalloidin labeling (red) of the organ of Corti shown in E , showing the actiniferous cuticular plate of OHCs (CP, arrow). G : Immunocytochemical control: negligible labeling in the rat organ of Corti was observed when blocking peptide was added to TRPV1 primary antibody incubation media. H : phalloidin labeling of the organ of Corti shown in G . The bar in H represents 50 µm and applies to A–H. I : specific TRPV1 immunolabeling occurs in the cell body of small-diameter DRG neurons. J : immunocytochemical control: negligible labeling in the DRG was observed when the TRPV1 primary antibody was replaced by BSA-PBS. K : immunocytochemical control: negligible labeling in the DRG was observed when blocking peptide was added to TRPV1 primary antibody incubation media. The bar in K represents 100 µm and applies to I–K .

    Article Snippet: The organ of Corti and DRG (positive control) of either the guinea pig or rats were dissected and fixed in the solution of 4% paraformaldehyde in 0.02 M PBS for 3 h. The fixed tissues were washed in 0.02 M PBS (pH 7.4), permeabilized with 0.5% Triton X-100 (Sigma) for 1 h and immunoblocked in 10% goat serum in 1% bovine albumin in 0.02 M PBS for 1 h. They were then incubated with rabbit anti-TRPV1 polyclonal antibody (gift from Dr. Caterina) diluted 1:1,000 in 1% BSA-PBS for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated with Alexa-488-conjugated goat anti-rabbit IgG for 3 h (Molecular Probes; 1:100 in 0.02 M PBS).

    Techniques: Immunolabeling, Expressing, Labeling, Blocking Assay, Incubation