Structured Review

New England Biolabs bsa i
Bsa I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bsa i/product/New England Biolabs
Average 99 stars, based on 22 article reviews
Price from $9.99 to $1999.99
bsa i - by Bioz Stars, 2020-01
99/100 stars

Images

Related Articles

Clone Assay:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations. .. The binary vector constructed, named as pHSE-CmelPDS (Fig. ), containing both guides, gRNA1 and gRNA2, was used to transform NEB 5-alpha Competent E. coli (High Efficiency; NEB).

Amplification:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: For the assembly of two gRNAs into pHSE401, a four-primer mixture with DT1-F0-PDS/DT2-R0-PDS and DT1-BsF-PDS/DT2-BsR-PDS in a proportion 1:20 (Table ), were used for PCR amplification along with pCBC-DT1T2 and Phusion High-Fidelity DNA Polymerase (NEB) following the manufacturer’s recommendations. .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The trunks containing the partially matched DNA sequences were ligation products of two DNA segments that were amplified from the plasmid pEGFP-N1. .. The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B).

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The trunks containing the two DNA target sequences were amplified from the modified plasmid pHY42. .. The pUC57-sgRNA expression vectors were linearized by Bsa I (NEB) and transcribed using the T7 High Efficiency Transcription Kit (TransGen Biotech).

Agarose Gel Electrophoresis:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: PCR protocol was 94 °C for 2 min, followed by 30 cycles of 94 °C for 15 s, 60 °C for 30 s, and 68 °C for 1 min and a final extension at 68 °C for 5 min. PCR product DT1T2-PCR (626 bp) was separated on 2% agarose gel and agarose purified with PureLink Quick Gel Extraction Kit (Invitrogen). .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Ligation:

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The trunks containing the partially matched DNA sequences were ligation products of two DNA segments that were amplified from the plasmid pEGFP-N1. .. The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B).

Isolation:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations. .. Positive clones were confirmed by Sanger sequencing, plasmids were isolated using GeneJET Plasmid Miniprep Kit (Thermo Scientific) and finally transformed into Agrobacterium tumefaciens EHA105.

Polymerase Chain Reaction:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: PCR protocol was 94 °C for 2 min, followed by 30 cycles of 94 °C for 15 s, 60 °C for 30 s, and 68 °C for 1 min and a final extension at 68 °C for 5 min. PCR product DT1T2-PCR (626 bp) was separated on 2% agarose gel and agarose purified with PureLink Quick Gel Extraction Kit (Invitrogen). .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: Arm 2 was PCR amplified from plasmid pBR322 using a biotin-labeled primer. .. The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B).

Construct:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: Vector construction Construct for constitutive expression of Cas9 was done following the protocol of Xing et al . .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B). .. Hairpin-capped trunk was constructed as previously described ( , ).

Purification:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: PCR protocol was 94 °C for 2 min, followed by 30 cycles of 94 °C for 15 s, 60 °C for 30 s, and 68 °C for 1 min and a final extension at 68 °C for 5 min. PCR product DT1T2-PCR (626 bp) was separated on 2% agarose gel and agarose purified with PureLink Quick Gel Extraction Kit (Invitrogen). .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The pUC57-sgRNA expression vectors were linearized by Bsa I (NEB) and transcribed using the T7 High Efficiency Transcription Kit (TransGen Biotech). .. The sgRNAs were then purified using the EasyPure RNA Purification Kit (TransGen Biotech).

Sequencing:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations. .. Positive clones were confirmed by Sanger sequencing, plasmids were isolated using GeneJET Plasmid Miniprep Kit (Thermo Scientific) and finally transformed into Agrobacterium tumefaciens EHA105.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The 1795-bp trunk containing the target DNA sequence was amplified from the plasmid pEGFP-N1 and digested with Alw NI (NEB). .. The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B).

Expressing:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: Vector construction Construct for constitutive expression of Cas9 was done following the protocol of Xing et al . .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: .. The pUC57-sgRNA expression vectors were linearized by Bsa I (NEB) and transcribed using the T7 High Efficiency Transcription Kit (TransGen Biotech). .. The sgRNAs were then purified using the EasyPure RNA Purification Kit (TransGen Biotech).

Modification:

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B). .. The trunks containing the two DNA target sequences were amplified from the modified plasmid pHY42.

Gel Extraction:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: PCR protocol was 94 °C for 2 min, followed by 30 cycles of 94 °C for 15 s, 60 °C for 30 s, and 68 °C for 1 min and a final extension at 68 °C for 5 min. PCR product DT1T2-PCR (626 bp) was separated on 2% agarose gel and agarose purified with PureLink Quick Gel Extraction Kit (Invitrogen). .. Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Transformation Assay:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations. .. Positive clones were confirmed by Sanger sequencing, plasmids were isolated using GeneJET Plasmid Miniprep Kit (Thermo Scientific) and finally transformed into Agrobacterium tumefaciens EHA105.

Plasmid Preparation:

Article Title: Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
Article Snippet: Paragraph title: Vector construction ... Then, DT1T2-PCR product was assembled into pHSE401 by Golden Gate cloning method, using Bsa I and T4 Ligase (NEB) following the manufacturer’s recommendations.

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The trunks containing the partially matched DNA sequences were ligation products of two DNA segments that were amplified from the plasmid pEGFP-N1. .. The upstream DNA segment was digested with Alw NI (NEB) and Bsa I (NEB), while the downstream segment was digested with Bas I (NEB) (fig. S1B).

Article Title: The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Article Snippet: The trunks containing the two DNA target sequences were amplified from the modified plasmid pHY42. .. The pUC57-sgRNA expression vectors were linearized by Bsa I (NEB) and transcribed using the T7 High Efficiency Transcription Kit (TransGen Biotech).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs n3041l bsa i hf
    N3041l Bsa I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n3041l bsa i hf/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n3041l bsa i hf - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    New England Biolabs bsa i
    Bsa I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa i/product/New England Biolabs
    Average 90 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    bsa i - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results