bsa i hfv2  (New England Biolabs)


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    New England Biolabs bsa i hfv2
    Bsa I Hfv2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzyme bsa i hf  (New England Biolabs)


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    New England Biolabs restriction enzyme bsa i hf
    Restriction Enzyme Bsa I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsa i hf v2  (New England Biolabs)


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    bsa i hf v2 neb r3733s  (New England Biolabs)


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    New England Biolabs bsa i hf v2 neb r3733s
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    bsa i hf  (New England Biolabs)


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    New England Biolabs bsa i hf
    Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA <t>and</t> <t>Bsa</t> <t>I</t> restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I
    Bsa I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression"

    Article Title: Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression

    Journal: Fungal Biology and Biotechnology

    doi: 10.1186/s40694-024-00175-4

    Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA and Bsa I restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I
    Figure Legend Snippet: Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA and Bsa I restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I

    Techniques Used: CRISPR, Plasmid Preparation, Sequencing, Expressing, Virus, Ligation

    bsa i hfv2  (New England Biolabs)


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    bsa i hfv2  (New England Biolabs)


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    bsa i golden gate reactions  (New England Biolabs)


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    bsa i hf  (New England Biolabs)


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    bsa i hf  (New England Biolabs)


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    New England Biolabs bsa i hf
    Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA <t>and</t> <t>Bsa</t> <t>I</t> restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I
    Bsa I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA <t>and</t> <t>Bsa</t> <t>I</t> restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I
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    Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA and Bsa I restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I

    Journal: Fungal Biology and Biotechnology

    Article Title: Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression

    doi: 10.1186/s40694-024-00175-4

    Figure Lengend Snippet: Overview on the CRISPR/Cas9 plasmid assembly. A . Scheme of the multiple overlap PCR to generate the 248 bp fragment. This fragment was assembled by PCR using two primers containing the sequence specific to the gRNA target and four generic primers. The obtained product includes a hammerhead ribozyme, the complementary sequence to the target DNA, the sequence for expression of the gRNA, a hepatitis delta virus (HDV) ribozyme for cutting at the 5’ and 3’ ends of the gRNA and Bsa I restriction sites on the 3’ and 5’ ends. B . Overview on the two GGAs to generate the final CRISPR/Cas9 plasmid. The initial GGA using Bsa I , leads to the ligation of the 248 bp-fragment and the BB1_L_23_syn_BsaI plasmid. The product of this assembly is then assembled with the plasmid bearing the Cas9 expression cassette via Bsb I

    Article Snippet: GGA were performed in a final volume of 20 µL containing 2 mM of each construct to assemble, CutSmart Buffer, 100 U of T4 ligase, 2 mM ATP, 30 U Bsa I-HF or Bbs I (New England Biolabs).

    Techniques: CRISPR, Plasmid Preparation, Sequencing, Expressing, Virus, Ligation