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Thermo Fisher broth
Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/broth/product/Thermo Fisher
Average 93 stars, based on 21 article reviews
Price from $9.99 to $1999.99
broth - by Bioz Stars, 2020-04
93/100 stars

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Acetylene Reduction Assay:

Article Title: Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity
Article Snippet: .. Escherichia coli XL1-Blue MRF′ [Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac ( F′ proAB lacI q ZΔM15 Tn 10 [Tetr ])], E. coli B strain BLR [F− ompT hsdSB (rB − mB − ) gal dcm Δ( srl-recA ) 306 ::Tn 10 (Tetr )], and E. coli AD494(DE3) [ ara leu-7697 lacX74 phoA PvuII phoR malF3 F′ ( lac + [ lacI q ) pro ) trxB :: kan (DE3)] (Novagen, Madison, Wis.) were used as recipients for cloning or production of recombinant proteins and were grown on Lennox Luria-Bertani (LB) agar or broth (Gibco-BRL, Gaithersburg, Md.) containing 40 μg of kanamycin (Sigma) per ml at 37°C for 18 h. .. To prepare the GBS whole-cell (WC) preparation used for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblots, approximately 15 mg (wet weight) of an overnight culture was added to 500 μl of sample buffer (0.2 M Tris-HCl [pH 6.8], 1% SDS, 2% mercaptoethanol, 10% glycerol, 0.001% bromophenol blue) and then boiled for 5 min, and after centrifugation, 15 μl of supernatant was applied to an SDS–10% PAGE gel.

Amplification:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin. .. To generate heterologous L. lactis expressing other PspC subtypes, the desired pspC DNA fragments were amplified by PCR using the chromosomal DNA of strain S. pneumoniae ATCC33400 (PspC2) or D39 (PspC3).

Clone Assay:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: Paragraph title: Culture Conditions, Cloning, and Recombinant Procedures in Lactococcus lactis ... L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin.

Article Title: Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity
Article Snippet: .. Escherichia coli XL1-Blue MRF′ [Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac ( F′ proAB lacI q ZΔM15 Tn 10 [Tetr ])], E. coli B strain BLR [F− ompT hsdSB (rB − mB − ) gal dcm Δ( srl-recA ) 306 ::Tn 10 (Tetr )], and E. coli AD494(DE3) [ ara leu-7697 lacX74 phoA PvuII phoR malF3 F′ ( lac + [ lacI q ) pro ) trxB :: kan (DE3)] (Novagen, Madison, Wis.) were used as recipients for cloning or production of recombinant proteins and were grown on Lennox Luria-Bertani (LB) agar or broth (Gibco-BRL, Gaithersburg, Md.) containing 40 μg of kanamycin (Sigma) per ml at 37°C for 18 h. .. To prepare the GBS whole-cell (WC) preparation used for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblots, approximately 15 mg (wet weight) of an overnight culture was added to 500 μl of sample buffer (0.2 M Tris-HCl [pH 6.8], 1% SDS, 2% mercaptoethanol, 10% glycerol, 0.001% bromophenol blue) and then boiled for 5 min, and after centrifugation, 15 μl of supernatant was applied to an SDS–10% PAGE gel.

Incubation:

Article Title: Structure-Activity Relationship (SAR) and Preliminary Mode of Action Studies of 3-Substituted Benzylthioquinolinium Iodide as Anti-opportunistic Infection Agents
Article Snippet: Microbial inocula were prepared after comparison of the absorbance (at 630 nm) of cell suspensions to the 0.5 McFarland standard and dilution of the suspensions in broth (Sabouraud dextrose and cation-adjusted MuellereHinton broth [Difco] for the fungi and bacteria, respectively, and 5% Alamar blue [BioSource International] in Middlebrook 7H9 broth with oleic acid albumin dextrose catalase enrichment for M. intracellulare ) to afford recommended inoculum sizes. .. The plates were read at either 530 nm or excitation and emission wavelengths of 544 and 590 nm (Alamar Blue method) prior to and after incubation.

Article Title: Occurrence of Plasmid- and Chromosome-Carried mcr-1 in Waterborne Enterobacteriaceae in China
Article Snippet: .. Mueller-Hinton agar and broth (Oxoid, Hampshire, UK) were used for all assays, and MIC results were determined following incubation at 35°C for 18 to 20 h. .. Primers specific for the β-lactamase genes, including bla TEM , bla SHV , group 1 bla CTX-M , group 2 bla CTX-M , group 8 bla CTX-M , and group 9 bla CTX-M , were used in this study ( ) ( ).

Article Title: Human norovirus binding to select bacteria representative of the human gut microbiota
Article Snippet: For the lactobacillus species, de Man, Rogosa and Sharpe agar or broth (MRS; Thermo Fisher Scientific) was used for cultivation. .. These plates were incubated anaerobically (5% carbon dioxide, 10% hydrogen, 85% nitrogen) in an anaerobic glove box with CAM-12 oxygen monitoring system and heating unit (Coy Laboratories, Grass Lake, MI) for 24 h at 37°C.

Expressing:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin. .. The recombinant pGKK1 plasmid was used for the inducible extracellular expression of PspC in L. lactis ( ).

Modification:

Article Title: Structure-Activity Relationship (SAR) and Preliminary Mode of Action Studies of 3-Substituted Benzylthioquinolinium Iodide as Anti-opportunistic Infection Agents
Article Snippet: Susceptibility testing was performed using a modified version of the NCCLS methods [ – ] for all organisms except for M. intracellulare , for which the modified Alamar blue procedure [ ] was followed. .. Microbial inocula were prepared after comparison of the absorbance (at 630 nm) of cell suspensions to the 0.5 McFarland standard and dilution of the suspensions in broth (Sabouraud dextrose and cation-adjusted MuellereHinton broth [Difco] for the fungi and bacteria, respectively, and 5% Alamar blue [BioSource International] in Middlebrook 7H9 broth with oleic acid albumin dextrose catalase enrichment for M. intracellulare ) to afford recommended inoculum sizes.

Article Title: Light-stimulated growth of proteorhodopsin-bearing sea-ice psychrophile Psychroflexus torquis is salinity dependent
Article Snippet: .. It was routinely grown in modified marine broth (5 g of proteose peptone (Oxoid, Adelaide, SA, Australia), 2 g of yeast extract (Oxoid) 35 g of sea salt (Red Sea) in 1 l of distilled water) aerobically at 5 °C. ..

Article Title: Isolation of Novel Afipia septicemium and Identification of Previously Unknown Bacteria Bradyrhizobium sp. OHSU_III from Blood of Patients with Poorly Defined Illnesses
Article Snippet: The cultures showing evidence of microbial growth were diluted 1∶2 or 1∶3 with fresh modified SP4 medium and expanded into new culture flasks. .. Aliquots of broth (∼ 100 µL) from cultures with possible microbial growth were also streaked out on 1% agar plates using SP4, BHI or YM broth as well as TSA- 5% sheep blood agar plates (Remel Inc., Lenexa, KS).

Transformation Assay:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin. .. Transformation with plasmid DNA was performed by electroporation as described recently ( ).

Sterility:

Article Title: Transforming Microbial Genotyping: A Robotic Pipeline for Genotyping Bacterial Strains
Article Snippet: After one day (S. enterica ) or one to seven days (Listeria ), single colonies were manually inoculated into 2-D bar-coded 1.4 ml screw-capped tubes (2D tubes) filled with 750 µl of broth (S. enterica : Oxoid tryptone soya broth [TSB], CM0129; Listeria : Oxoid brain heart infusion broth [BHI], CM225B). (Some experiments were performed with other volumes ranging from 500–1000 µl). .. Prior to manual inoculation, the 1.4 ml 2D tubes were filled with liquid media using LHS1 ( ) and incubated overnight to ensure sterility.

Derivative Assay:

Article Title: Human norovirus binding to select bacteria representative of the human gut microbiota
Article Snippet: For the lactobacillus species, de Man, Rogosa and Sharpe agar or broth (MRS; Thermo Fisher Scientific) was used for cultivation. .. Each of the isolated fecally derived cultures underwent further characterization using Gram stain, motility tests and the Oxoid Microbact GNB 24E reagent system (Thermo Fisher Scientific).

Electroporation:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin. .. Transformation with plasmid DNA was performed by electroporation as described recently ( ).

Northern Blot:

Article Title: Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity
Article Snippet: These strains were obtained from the American Type Culture Collection (Rockville, Md.), centre de recherche en infectiologie of the Centre Hospitalier de l'Université Laval (Ste-Foy, Canada), Children's Hospital and Medical Center (Seattle, Wash.), Laboratoire de Santé Publique du Québec (Montreal, Canada), and National Centre for Streptococcus, Provincial Laboratory of Public Health for Northern Alberta (Edmonton, Canada). .. Escherichia coli XL1-Blue MRF′ [Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac ( F′ proAB lacI q ZΔM15 Tn 10 [Tetr ])], E. coli B strain BLR [F− ompT hsdSB (rB − mB − ) gal dcm Δ( srl-recA ) 306 ::Tn 10 (Tetr )], and E. coli AD494(DE3) [ ara leu-7697 lacX74 phoA PvuII phoR malF3 F′ ( lac + [ lacI q ) pro ) trxB :: kan (DE3)] (Novagen, Madison, Wis.) were used as recipients for cloning or production of recombinant proteins and were grown on Lennox Luria-Bertani (LB) agar or broth (Gibco-BRL, Gaithersburg, Md.) containing 40 μg of kanamycin (Sigma) per ml at 37°C for 18 h.

Cell Culture:

Article Title: Deciphering Adaptation Strategies of the Epidemic Clostridium difficile 027 Strain during Infection through In Vivo Transcriptional Analysis
Article Snippet: .. Bacterial strains and growth conditions Strain R20291 (Wild-type BI/NAP1/027 Stoke Mandeville isolate, gift from N. Minton, University of Notthingham, UK) was cultured in peptone yeast (PY) agar or broth (Oxoid) in an anaerobic atmosphere (10% CO2 , 10% H2 , 80% N2 ) at 37°C. .. Sporulation assays were performed from cell cultures grown in BHIS (brain heart infusion containing 5 mg ml-1 yeast extract [AES] and 0.1% [w/v] L-cysteine [Merck]).

Article Title: Studies on the effect of Lactococcus garvieae of dairy origin on both cheese and Nile tilapia (O. niloticus)
Article Snippet: The culture was grown in broth (brain heart infusion; Oxoid) at 37 °C for 18–24 h. L. garvieae strain was obtained from our previous work (we chose the strain that carry only Fbp gene) in which it was isolated, identified, and stored frozen at −80 °C in broth culture contained 15% (w/v) glycerol. .. Then it was cultured on M17 medium anaerobically at 37 °C for 48 h.

other:

Article Title: Inclusion of prebiotics on the viability of a commercial Lactobacillus casei subsp. rhamnosus culture in a tropical fruit beverage
Article Snippet: Culture medium and broth (de Man–Rogosa–Sharpe, MRS; Oxoid® ) were prepared according to the manufacturer’s instructions.

Article Title: Attenuation of Listeria monocytogenes Virulence by Cannabis sativa L. Essential Oil
Article Snippet: Blood agar base (BAB) supplemented with 5% sheep blood, Müller-Hinton agar (MHA) supplemented with 5% sheep blood, Müller-Hinton cation-adjusted broth (CAMHB) supplemented with 3% laked sheep blood, brain heart infusion (BHI) agar and broth, Tryptone Soya Broth (TSB), and Luria Bertani (LB) agar and broth (all from Oxoid, Basingstoke, UK) were used in the study.

Article Title: Paeniclostridium sordellii and Clostridioides difficile encode similar and clinically relevant tetracycline resistance loci in diverse genomic locations
Article Snippet: Unless stated otherwise, bacterial cultures were grown in HIS broth (37 g/L Heart Infusion (Oxoid), 5 g/L yeast extract, 1 g/L L-Cysteine, 0.375% (w /v ) glucose) or on HIS agar (HIS broth with 15 g/L agar) at 37 °C in an anaerobic chamber (Coy Laboratory Products, Inc.) in an atmosphere of 10% H2 , 10% CO2 and 80% N2 .

Polymerase Chain Reaction:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin. .. To generate heterologous L. lactis expressing other PspC subtypes, the desired pspC DNA fragments were amplified by PCR using the chromosomal DNA of strain S. pneumoniae ATCC33400 (PspC2) or D39 (PspC3).

Recombinant:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: Paragraph title: Culture Conditions, Cloning, and Recombinant Procedures in Lactococcus lactis ... L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin.

Article Title: Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity
Article Snippet: .. Escherichia coli XL1-Blue MRF′ [Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac ( F′ proAB lacI q ZΔM15 Tn 10 [Tetr ])], E. coli B strain BLR [F− ompT hsdSB (rB − mB − ) gal dcm Δ( srl-recA ) 306 ::Tn 10 (Tetr )], and E. coli AD494(DE3) [ ara leu-7697 lacX74 phoA PvuII phoR malF3 F′ ( lac + [ lacI q ) pro ) trxB :: kan (DE3)] (Novagen, Madison, Wis.) were used as recipients for cloning or production of recombinant proteins and were grown on Lennox Luria-Bertani (LB) agar or broth (Gibco-BRL, Gaithersburg, Md.) containing 40 μg of kanamycin (Sigma) per ml at 37°C for 18 h. .. To prepare the GBS whole-cell (WC) preparation used for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblots, approximately 15 mg (wet weight) of an overnight culture was added to 500 μl of sample buffer (0.2 M Tris-HCl [pH 6.8], 1% SDS, 2% mercaptoethanol, 10% glycerol, 0.001% bromophenol blue) and then boiled for 5 min, and after centrifugation, 15 μl of supernatant was applied to an SDS–10% PAGE gel.

Isolation:

Article Title: Light-stimulated growth of proteorhodopsin-bearing sea-ice psychrophile Psychroflexus torquis is salinity dependent
Article Snippet: Bacterial strains and media P. torquis ATCC 700755T was originally isolated from multi-year sea ice collected from Prydz Bay, Vestfold Hills, Antarctica (S 68°36′30′′, E 78°04′19′′) ( ). .. It was routinely grown in modified marine broth (5 g of proteose peptone (Oxoid, Adelaide, SA, Australia), 2 g of yeast extract (Oxoid) 35 g of sea salt (Red Sea) in 1 l of distilled water) aerobically at 5 °C.

Article Title: Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity
Article Snippet: The sip gene was initially identified from GBS strain C388/90 (Ia/c), which had been isolated from the cerebrospinal fluid of a child with meningitis and was obtained from the Children's Hospital of Eastern Ontario (Ottawa, Canada). .. Escherichia coli XL1-Blue MRF′ [Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac ( F′ proAB lacI q ZΔM15 Tn 10 [Tetr ])], E. coli B strain BLR [F− ompT hsdSB (rB − mB − ) gal dcm Δ( srl-recA ) 306 ::Tn 10 (Tetr )], and E. coli AD494(DE3) [ ara leu-7697 lacX74 phoA PvuII phoR malF3 F′ ( lac + [ lacI q ) pro ) trxB :: kan (DE3)] (Novagen, Madison, Wis.) were used as recipients for cloning or production of recombinant proteins and were grown on Lennox Luria-Bertani (LB) agar or broth (Gibco-BRL, Gaithersburg, Md.) containing 40 μg of kanamycin (Sigma) per ml at 37°C for 18 h.

Article Title: Human norovirus binding to select bacteria representative of the human gut microbiota
Article Snippet: For the lactobacillus species, de Man, Rogosa and Sharpe agar or broth (MRS; Thermo Fisher Scientific) was used for cultivation. .. Unique colonies from each agar plate (i.e. size, color, shape, hemolysis) were isolated, purified, and grown anaerobically at 37°C overnight on both the agar and in the broth formulations from which they were initially isolated.

Article Title: Determination of the Duration of Antibacterial Efficacy following Administration of Gamithromycin Using a Bovine Mannheimia haemolytica Challenge Model ▿
Article Snippet: The 37 animals remaining on day 0 were each challenged with approximately 300 ml of an M. haemolytica A1 phosphate-buffered saline diluted broth (nutrient broth 2; Oxoid) culture containing 2.33 × 105 CFU of M. haemolytica A1/ml by endobronchial deposition at the bifurcation of the main bronchus through a fiber-optic bronchoscope. .. The challenge strain was isolated from a pneumonic calf in 1993, and its MIC of gamithromycin was confirmed to be 1 μg/ml prior to the start of this study.

Article Title: Studies on the effect of Lactococcus garvieae of dairy origin on both cheese and Nile tilapia (O. niloticus)
Article Snippet: .. The culture was grown in broth (brain heart infusion; Oxoid) at 37 °C for 18–24 h. L. garvieae strain was obtained from our previous work (we chose the strain that carry only Fbp gene) in which it was isolated, identified, and stored frozen at −80 °C in broth culture contained 15% (w/v) glycerol. .. Then it was cultured on M17 medium anaerobically at 37 °C for 48 h.

Microscopy:

Article Title: Isolation of Novel Afipia septicemium and Identification of Previously Unknown Bacteria Bradyrhizobium sp. OHSU_III from Blood of Patients with Poorly Defined Illnesses
Article Snippet: The broths in culture flasks were checked for color changes and examined for evidence of microbial growth by inverted phase microscopy 2 to 3 times a week. .. Aliquots of broth (∼ 100 µL) from cultures with possible microbial growth were also streaked out on 1% agar plates using SP4, BHI or YM broth as well as TSA- 5% sheep blood agar plates (Remel Inc., Lenexa, KS).

Purification:

Article Title: Human norovirus binding to select bacteria representative of the human gut microbiota
Article Snippet: For the lactobacillus species, de Man, Rogosa and Sharpe agar or broth (MRS; Thermo Fisher Scientific) was used for cultivation. .. Unique colonies from each agar plate (i.e. size, color, shape, hemolysis) were isolated, purified, and grown anaerobically at 37°C overnight on both the agar and in the broth formulations from which they were initially isolated.

IA:

Article Title: Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity
Article Snippet: The sip gene was initially identified from GBS strain C388/90 (Ia/c), which had been isolated from the cerebrospinal fluid of a child with meningitis and was obtained from the Children's Hospital of Eastern Ontario (Ottawa, Canada). .. Escherichia coli XL1-Blue MRF′ [Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac ( F′ proAB lacI q ZΔM15 Tn 10 [Tetr ])], E. coli B strain BLR [F− ompT hsdSB (rB − mB − ) gal dcm Δ( srl-recA ) 306 ::Tn 10 (Tetr )], and E. coli AD494(DE3) [ ara leu-7697 lacX74 phoA PvuII phoR malF3 F′ ( lac + [ lacI q ) pro ) trxB :: kan (DE3)] (Novagen, Madison, Wis.) were used as recipients for cloning or production of recombinant proteins and were grown on Lennox Luria-Bertani (LB) agar or broth (Gibco-BRL, Gaithersburg, Md.) containing 40 μg of kanamycin (Sigma) per ml at 37°C for 18 h.

Plasmid Preparation:

Article Title: The Choline-binding Protein PspC of Streptococcus pneumoniae Interacts with the C-terminal Heparin-binding Domain of Vitronectin *
Article Snippet: L. lactis MG1363 was grown statically at 30 °C in M17 agar or broth (Oxoid) supplemented with 0.5% glucose and 5 μg/ml erythromycin. .. Transformation with plasmid DNA was performed by electroporation as described recently ( ).

Irradiation:

Article Title: Isolation of Novel Afipia septicemium and Identification of Previously Unknown Bacteria Bradyrhizobium sp. OHSU_III from Blood of Patients with Poorly Defined Illnesses
Article Snippet: Microbial cultures Cryopreserved SP4 broth (100 to 200 µL) containing cultures established from blood samples were inoculated into 25 cm2 tissue culture flasks containing ∼ 7 mL of modified SP4 medium supplemented with 10% irradiated fetal bovine serum (FBS). .. Aliquots of broth (∼ 100 µL) from cultures with possible microbial growth were also streaked out on 1% agar plates using SP4, BHI or YM broth as well as TSA- 5% sheep blood agar plates (Remel Inc., Lenexa, KS).

Selection:

Article Title: Defined Deletion Mutants Demonstrate that the Major Secreted Toxins Are Not Essential for the Virulence of Aeromonas salmonicida
Article Snippet: A. salmonicida strains were grown at 15 to 18°C in tryptic soy agar (TSA) or broth (tryptic soy broth [TSB; Oxoid]) supplemented with Davis minimal medium ( ). .. Media used for screening or selection included egg yolk emulsion (EYE) (20% [vol/vol] egg yolk in 10% [wt/vol] NaCl), egg yolk agar (EYA) (10% [vol/vol] EYE in salt-free nutrient agar), skim milk (50% [vol/vol]) in salt-free nutrient agar (SMA), sucrose (15% [wt/vol]) in TSA (TSAS), and Congo Red (50 μg/ml) in TSA (CRA).

In Vitro:

Article Title: Structure-Activity Relationship (SAR) and Preliminary Mode of Action Studies of 3-Substituted Benzylthioquinolinium Iodide as Anti-opportunistic Infection Agents
Article Snippet: Compounds were evaluated in vitro against a panel of microorganisms including C. albicans ATCC 90028 ( Ca ), Candida krusei ATCC 6258 ( Ck ), C. neoformans ATCC 90113 ( Cn ), S. aureus ATCC 29213 ( Sa ), Methicillin-resistant S. aureus ATCC 33591( MRSA ), A. fumigatus ATCC 204305 ( Af ), and M. intracellulare ATCC 23068 ( Mi ) as previously reported [ ] All organisms were obtained from the American Type Culture Collection (Manassas, Va.). .. Microbial inocula were prepared after comparison of the absorbance (at 630 nm) of cell suspensions to the 0.5 McFarland standard and dilution of the suspensions in broth (Sabouraud dextrose and cation-adjusted MuellereHinton broth [Difco] for the fungi and bacteria, respectively, and 5% Alamar blue [BioSource International] in Middlebrook 7H9 broth with oleic acid albumin dextrose catalase enrichment for M. intracellulare ) to afford recommended inoculum sizes.

Article Title: Occurrence of Plasmid- and Chromosome-Carried mcr-1 in Waterborne Enterobacteriaceae in China
Article Snippet: In vitro antimicrobial susceptibility testing was performed to examine the susceptibility of the original isolates and the transconjugants to imipenem (IPM), gentamicin (GEN), cefotaxime (CTX), piperacillin-tazobactam (TZP), cefoperazone/sulbactam (SCF), amikacin (AMK), ticarcillin-clavulanic acid (TIM), cefepime (FEP), ceftazidime (CAZ), ceftriaxone (CRO), and ciprofloxacin (CIP). .. Mueller-Hinton agar and broth (Oxoid, Hampshire, UK) were used for all assays, and MIC results were determined following incubation at 35°C for 18 to 20 h.

Staining:

Article Title: Human norovirus binding to select bacteria representative of the human gut microbiota
Article Snippet: For the lactobacillus species, de Man, Rogosa and Sharpe agar or broth (MRS; Thermo Fisher Scientific) was used for cultivation. .. Each of the isolated fecally derived cultures underwent further characterization using Gram stain, motility tests and the Oxoid Microbact GNB 24E reagent system (Thermo Fisher Scientific).

Chick Chorioallantoic Membrane Assay:

Article Title: Human norovirus binding to select bacteria representative of the human gut microbiota
Article Snippet: For the lactobacillus species, de Man, Rogosa and Sharpe agar or broth (MRS; Thermo Fisher Scientific) was used for cultivation. .. These plates were incubated anaerobically (5% carbon dioxide, 10% hydrogen, 85% nitrogen) in an anaerobic glove box with CAM-12 oxygen monitoring system and heating unit (Coy Laboratories, Grass Lake, MI) for 24 h at 37°C.

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  • 99
    Thermo Fisher tryptic soy broth tsb glu
    Desiccation survival of L. monocytogenes 568 and 08-5578 on a food grade stainless steel surface. Cells were pre-cultured at 15°C in <t>TSB-glu</t> and re-suspended in TSB-glu before being desiccated (43% RH, 15°C) for 48 h with a starting concentration of 8 × 10 7 CFU/cm 2 . Cells were enumerated by plate counts. Symbols are averages of three biological independent experiments with triplicates ( n = 9). Error bars indicate standard deviation.
    Tryptic Soy Broth Tsb Glu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of pH on growth and AL biofilm formation in <t>SOBG</t> broth at <t>27°C.</t> (A) Growth of wild type (Pcc PC1) at indicated pHs. (B) AL biofilm formation by the wild type (Pcc PC1) and the Δ cytR mutant. The values are mean and error bars indicate standard deviations ( ± ) of three independent experiments.
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    Thermo Fisher tryptone yeast extract glucose broth
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    Thermo Fisher camh broth
    Real-time data showing the effect of ( a ) Tobramycin alone (1.0–32.0 µg/mL) and ( b ) Tobramycin (1.0–32.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa <t>CCIN34519</t> biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth <t>(CAMH)</t> while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.
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    Desiccation survival of L. monocytogenes 568 and 08-5578 on a food grade stainless steel surface. Cells were pre-cultured at 15°C in TSB-glu and re-suspended in TSB-glu before being desiccated (43% RH, 15°C) for 48 h with a starting concentration of 8 × 10 7 CFU/cm 2 . Cells were enumerated by plate counts. Symbols are averages of three biological independent experiments with triplicates ( n = 9). Error bars indicate standard deviation.

    Journal: Frontiers in Microbiology

    Article Title: Initial Transcriptomic Response and Adaption of Listeria monocytogenes to Desiccation on Food Grade Stainless Steel

    doi: 10.3389/fmicb.2019.03132

    Figure Lengend Snippet: Desiccation survival of L. monocytogenes 568 and 08-5578 on a food grade stainless steel surface. Cells were pre-cultured at 15°C in TSB-glu and re-suspended in TSB-glu before being desiccated (43% RH, 15°C) for 48 h with a starting concentration of 8 × 10 7 CFU/cm 2 . Cells were enumerated by plate counts. Symbols are averages of three biological independent experiments with triplicates ( n = 9). Error bars indicate standard deviation.

    Article Snippet: Desiccation of L. monocytogenes on Stainless Steel Prior to desiccation survival experiments, both strains were pre-cultured in 50 ml Tryptic Soy Broth (TSB-glu) [TSB supplemented with 1% (w/v) glucose (Thermo Fisher Scientific, Loughborough, United Kingdom)] for 48 h at 15°C.

    Techniques: Cell Culture, Concentration Assay, Standard Deviation

    Effect of pH on growth and AL biofilm formation in SOBG broth at 27°C. (A) Growth of wild type (Pcc PC1) at indicated pHs. (B) AL biofilm formation by the wild type (Pcc PC1) and the Δ cytR mutant. The values are mean and error bars indicate standard deviations ( ± ) of three independent experiments.

    Journal: Frontiers in Microbiology

    Article Title: CytR Homolog of Pectobacterium carotovorum subsp. carotovorum Controls Air-Liquid Biofilm Formation by Regulating Multiple Genes Involved in Cellulose Production, c-di-GMP Signaling, Motility, and Type III Secretion System in Response to Nutritional and Environmental Signals

    doi: 10.3389/fmicb.2017.00972

    Figure Lengend Snippet: Effect of pH on growth and AL biofilm formation in SOBG broth at 27°C. (A) Growth of wild type (Pcc PC1) at indicated pHs. (B) AL biofilm formation by the wild type (Pcc PC1) and the Δ cytR mutant. The values are mean and error bars indicate standard deviations ( ± ) of three independent experiments.

    Article Snippet: 107 CFU/mL) was suspended in glass test tubes containing 5 mL SOBG broth and incubated at 27°C in anaerobic chamber (Thermo, Inc., Portsmouth, NH, United States) for 7 days ( Figure ).

    Techniques: Periodic Counter-current Chromatography, Mutagenesis

    Impacts of osmotic stress on growth and AL biofilm formation in SOBG broth at 27°C. (A) Growth of wild type in SOBG containing indicated concentrations of NaCl. (B) AL biofilm formation by the wild type (Pcc PC1) and the Δ cytR mutant in different concentrations of NaCl. (C) No biofilm formed by the wild type and the Δ cytR mutant in anaerobic condition. These experiments were repeated at least three times. The values are mean and error bars indicate standard deviations ( ± ) of three independent experiments.

    Journal: Frontiers in Microbiology

    Article Title: CytR Homolog of Pectobacterium carotovorum subsp. carotovorum Controls Air-Liquid Biofilm Formation by Regulating Multiple Genes Involved in Cellulose Production, c-di-GMP Signaling, Motility, and Type III Secretion System in Response to Nutritional and Environmental Signals

    doi: 10.3389/fmicb.2017.00972

    Figure Lengend Snippet: Impacts of osmotic stress on growth and AL biofilm formation in SOBG broth at 27°C. (A) Growth of wild type in SOBG containing indicated concentrations of NaCl. (B) AL biofilm formation by the wild type (Pcc PC1) and the Δ cytR mutant in different concentrations of NaCl. (C) No biofilm formed by the wild type and the Δ cytR mutant in anaerobic condition. These experiments were repeated at least three times. The values are mean and error bars indicate standard deviations ( ± ) of three independent experiments.

    Article Snippet: 107 CFU/mL) was suspended in glass test tubes containing 5 mL SOBG broth and incubated at 27°C in anaerobic chamber (Thermo, Inc., Portsmouth, NH, United States) for 7 days ( Figure ).

    Techniques: Periodic Counter-current Chromatography, Mutagenesis

    In vitro growth of C. difficile ATTC BAA-1870 in varying Na + and pH conditions. [Na + ] ranges for healthy and CDI stool are displayed as bars along the x -axis. A : growth (CFU) of C. difficile in tryptone-yeast extract-glucose (TYG) broth at varying [Na

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Human Clostridium difficile infection: inhibition of NHE3 and microbiota profile

    doi: 10.1152/ajpgi.00090.2014

    Figure Lengend Snippet: In vitro growth of C. difficile ATTC BAA-1870 in varying Na + and pH conditions. [Na + ] ranges for healthy and CDI stool are displayed as bars along the x -axis. A : growth (CFU) of C. difficile in tryptone-yeast extract-glucose (TYG) broth at varying [Na

    Article Snippet: B. thetaiotaomicron was grown in TSB (Fisher Scientific), and C. difficile was grown in tryptone-yeast extract-glucose broth (TYG; Thermo Fisher Scientific) at 37°C in a Coy Systems, dual-port anaerobic chamber (Coy Laboratory Products, Grass Lake, MI).

    Techniques: In Vitro

    Real-time data showing the effect of ( a ) Tobramycin alone (1.0–32.0 µg/mL) and ( b ) Tobramycin (1.0–32.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Tobramycin alone (1.0–32.0 µg/mL) and ( b ) Tobramycin (1.0–32.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Ertapenem alone (5.0–40.0 µg/mL) and ( b ) Ertapenem (5.0–40.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Ertapenem alone (5.0–40.0 µg/mL) and ( b ) Ertapenem (5.0–40.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Ceftriaxone alone (40–640 µg/mL) and ( b ) Ceftriaxone (40–640 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Ceftriaxone alone (40–640 µg/mL) and ( b ) Ceftriaxone (40–640 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Moxifloxacin alone (1.0–32.0 µg/mL) and ( b ) Moxifloxacin (1.0–32.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Moxifloxacin alone (1.0–32.0 µg/mL) and ( b ) Moxifloxacin (1.0–32.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Norfloxacin alone (0.63–10.0 µg/mL) and ( b ) Norfloxacin (0.63–10.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Norfloxacin alone (0.63–10.0 µg/mL) and ( b ) Norfloxacin (0.63–10.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Gentamicin alone (1.56–50.0 µg/mL) and ( b ) Gentamicin (1.56–50.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Gentamicin alone (1.56–50.0 µg/mL) and ( b ) Gentamicin (1.56–50.0 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Levofloxacin alone (0.13–8.00 µg/mL) and ( b ) Levofloxacin (0.13–8.00 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Levofloxacin alone (0.13–8.00 µg/mL) and ( b ) Levofloxacin (0.13–8.00 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques:

    Real-time data showing the effect of ( a ) Ciprofloxacin alone (0.1–1.6 µg/mL) and ( b ) Ciprofloxacin (0.1–1.6 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Journal: Antibiotics

    Article Title: In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

    doi: 10.3390/antibiotics3010064

    Figure Lengend Snippet: Real-time data showing the effect of ( a ) Ciprofloxacin alone (0.1–1.6 µg/mL) and ( b ) Ciprofloxacin (0.1–1.6 µg/mL) in combination with zinc sulfate (2.5 mM) on P . aeruginosa CCIN34519 biofilm as monitored by the Bioscreen C over 96 h. Control represents cation adjusted Mueller Hinton II broth (CAMH) while, ZnSO 4 indicates CAMH broth supplemented with 2.5 mM zinc sulfate.

    Article Snippet: Scanning Electron Microscopy (SEM) For visualization of P. a eruginosa CCIN34519 biofilm, overnight broth culture of P. aeruginosa CCIN34519 in CAMH broth was diluted in PBS to 1.5 × 108 CFU/mL using 0.5 McFarland equivalence turbidity standards (Thermo Scientific Remel™, Lenexa, KS, USA).

    Techniques: