Structured Review

Merck KGaA broth
Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/broth/product/Merck KGaA
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
broth - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Clone Assay:

Article Title: An Asymmetric Model for Na+-translocating Glutaconyl-CoA Decarboxylases *
Article Snippet: .. For cloning, sequencing, and gene expression Escherichia coli strains DH5α (Invitrogen, Karlsruhe, Germany) ( ) and RosettaTM (DE3)pLysS (Novagen, Darmstadt, Germany) ( ) were grown in Luria-Bertani (LB) medium or standard I nutrient broth (Merck, Darmstadt, Germany) supplemented with the required antibiotics at 37 °C. .. The purification of the glutaconyl-CoA decarboxylase complex was carried out as previously described ( ).

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: The PCR product was cloned into the p- ENTRY vector and introduced into Escherichia coli DH5α by chemical transformation. .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml).

Centrifugation:

Article Title: Effect of High-Pressure-Induced Ice I-to-Ice III Phase Transitions on Inactivation of Listeria innocua in Frozen Suspension
Article Snippet: One bead from a deep-frozen culture was transferred into standard I nutrient broth (Merck, Darmstadt, Germany), which was incubated at 30°C for 24 h. This broth was used to inoculate the final broth with a cell count of about 103 CFU/ml, which was incubated again at 30°C for 24 h to obtain bacteria in the stationary growth phase with a cell count of about 109 CFU/ml. .. This culture was harvested by centrifugation at 5,200 × g for 15 min at room temperature and resuspended in phosphate-buffered saline (PBS) to the same volume.

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml). .. Cell debris was removed by centrifugation at 30,000 × g for 1 h and 4 °C to obtain 34 ml of cell extract containing 34 mg of protein/ml.

Sequencing:

Article Title: An Asymmetric Model for Na+-translocating Glutaconyl-CoA Decarboxylases *
Article Snippet: .. For cloning, sequencing, and gene expression Escherichia coli strains DH5α (Invitrogen, Karlsruhe, Germany) ( ) and RosettaTM (DE3)pLysS (Novagen, Darmstadt, Germany) ( ) were grown in Luria-Bertani (LB) medium or standard I nutrient broth (Merck, Darmstadt, Germany) supplemented with the required antibiotics at 37 °C. .. The purification of the glutaconyl-CoA decarboxylase complex was carried out as previously described ( ).

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: After confirmation of the desired sequence, the genes were transferred from the donor vector to expression vector pASG IBA33 and further into E. coli BL21(GroEL). .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml).

Article Title: Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
Article Snippet: Despite sharing over 99 % sequence similarity with each other in 16S rRNA genes (Ibrahim et al. ), these isolates showed some differences in aggregates formation, colony morphology, color, and size. .. These isolates show fast growth and shorter cell length without aggregation when grown in rich media; nutrient broth and standard I nutrient broth (Merck KGaA) (Ibrahim et al. ).

Sample Prep:

Article Title: Effect of High-Pressure-Induced Ice I-to-Ice III Phase Transitions on Inactivation of Listeria innocua in Frozen Suspension
Article Snippet: Paragraph title: Sample preparation. ... One bead from a deep-frozen culture was transferred into standard I nutrient broth (Merck, Darmstadt, Germany), which was incubated at 30°C for 24 h. This broth was used to inoculate the final broth with a cell count of about 103 CFU/ml, which was incubated again at 30°C for 24 h to obtain bacteria in the stationary growth phase with a cell count of about 109 CFU/ml.

Positive Control:

Article Title: Predicting the influence of multiple components on microbial inhibition using a logistic response model - a novel approach
Article Snippet: The stock solutions were serially diluted in enriched CASO broth (Merck Chemicals (Pty) Ltd) for the bacteria and Sabouraud Dextrose 4% broth (Merck Chemicals (Pty) Ltd) for Candida ; in the 96-well microtiter-plate adding 100 μl of a McFarland Standard 1 inoculum of 48 hour old microorganisms grown at 37˚C. .. The final concentration of the extract and TEAVIGO™ ranged from 0.10–12.5 mg/ml and the positive control, 1.25% v/v chlorhexidine gluconate (CHX) (Dental Warehouse, Sandton, South Africa), ranged from 4.77 × 10-6 –0.31% v/v.

Polymerase Chain Reaction:

Article Title: Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate: a potential host for the expression of heterologous proteins
Article Snippet: S. enterica , P. fluorescens and A. hydrophila were cultured in tryptic soy agar or broth (pH: 7.3 ± 0.2) (Merck, Germany) and B. cereus in nutrient agar or broth (pH: 7.0) (Merck, Germany). .. PCR reactions were performed in a PCR Master Cycler (Eppendorf, Germany) with Pfu DNA polymerase (Promega Corp., Madison, WI, USA), as recommended by the polymerase supplier.

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: The PCR product was cloned into the p- ENTRY vector and introduced into Escherichia coli DH5α by chemical transformation. .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml).

Isolation:

Article Title: Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide
Article Snippet: Paragraph title: 5.1. Isolation and Identification of Leuconostoc mesenteroides N3 ... The media including the de Man–Rogosa–Sharpe (MRS) agar or broth (Merck, Darmstadt, Germany) were used.

Incubation:

Article Title: Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs
Article Snippet: .. The yeast species were routinely cultivated in worth broth (Merck KGaA, Darmstadt, Germany) and incubated for 48 h at 25 °C. .. For starvation of the cells, the biomass was transferred to tryptone (6 g/L; Oxoid Ltd., Hampshire, UK) at a dilution ratio of 1:100.

Article Title: Quantification of Endospore-Forming Firmicutes by Quantitative PCR with the Functional Gene spo0A
Article Snippet: The media used were standard nutrient broth (NB); DSM medium ( ); standard I nutrient broth (ST1; Merck Millipore, Darmstadt, Germany); PTYG medium composed of 0.25 g/liter peptone, 0.25 g/liter tryptone, 0.5 g/liter yeast extract, 0.5 g/liter glucose, 0.6 g/liter MgSO4 ·7H2 O, 0.07 g/liter CaCl2 ·2 H2 O (pH was adjusted with NaOH and H2 SO4 ); FeSO medium (described elsewhere [ ]); Difco2216*, corresponding to a modified version of marine broth (also described elsewhere [ ]) supplemented with 20 mM sodium pyruvate and 1 mg/liter of resazurin to verify anoxia. .. The incubation was at 37°C under anaerobic conditions attained by placing the plates in sealed plastic bags in an Anaerocult A mini apparatus and one strip of Anaerotest (Merck Millipore).

Article Title: Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide
Article Snippet: The media including the de Man–Rogosa–Sharpe (MRS) agar or broth (Merck, Darmstadt, Germany) were used. .. A volume of 1 mL was plated on MRS agar and incubated in CO2 (5%) conditions at 45 °C for 48 h. About ten colonies were grown at the incubated condition.

Article Title: Effect of High-Pressure-Induced Ice I-to-Ice III Phase Transitions on Inactivation of Listeria innocua in Frozen Suspension
Article Snippet: .. One bead from a deep-frozen culture was transferred into standard I nutrient broth (Merck, Darmstadt, Germany), which was incubated at 30°C for 24 h. This broth was used to inoculate the final broth with a cell count of about 103 CFU/ml, which was incubated again at 30°C for 24 h to obtain bacteria in the stationary growth phase with a cell count of about 109 CFU/ml. .. This culture was harvested by centrifugation at 5,200 × g for 15 min at room temperature and resuspended in phosphate-buffered saline (PBS) to the same volume.

Article Title: Comparative Genome Sequencing Reveals Within-Host Genetic Changes in Neisseria meningitidis during Invasive Disease
Article Snippet: Bacterial strains Upon receipt at the NRZMHi, all samples are routinely streaked out on Martin-Lewis agar plates (Becton Dickinson, Heidelberg, Germany) and incubated overnight at 35°C in 5% CO2 . .. The next day, a sweep collecting all colonies grown on the agar plate was taken and frozen at -70°C in standard I nutrient broth (25 g/l) (Merck Millipore, Darmstadt, Germany) supplemented with 20% glycerol without further processing.

Cell Culture:

Article Title: Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate: a potential host for the expression of heterologous proteins
Article Snippet: .. S. enterica , P. fluorescens and A. hydrophila were cultured in tryptic soy agar or broth (pH: 7.3 ± 0.2) (Merck, Germany) and B. cereus in nutrient agar or broth (pH: 7.0) (Merck, Germany). .. All strains were maintained as stock cultures at −80°C in the respective cultivation broth containing 20% (v/v) glycerol (Merck, Germany).

Purification:

Article Title: Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
Article Snippet: From different samples collected at various positions inside the compost heap, six thermophilic PHB-accumulating isolates, MW9T , MW10, MW11, MW12, MW13 and MW14 were purified and characterized (Ibrahim et al. ). .. These isolates show fast growth and shorter cell length without aggregation when grown in rich media; nutrient broth and standard I nutrient broth (Merck KGaA) (Ibrahim et al. ).

Produced:

Article Title: Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs
Article Snippet: To obtain a more realistic profile, sucrose and maltose were added, which are usually included intentionally or are produced by amylases in wheat dough. .. The yeast species were routinely cultivated in worth broth (Merck KGaA, Darmstadt, Germany) and incubated for 48 h at 25 °C.

Concentration Assay:

Article Title: Predicting the influence of multiple components on microbial inhibition using a logistic response model - a novel approach
Article Snippet: Antimicrobial susceptibility testing To determine the effects of combinations of H. natalensis , M. alternifolia essential oil, M. piperita essential oil and TEAVIGO™, the minimum inhibitory concentration (MIC) of each component was determined first using the anti-microbial microtiter-plate method [ ]. .. The stock solutions were serially diluted in enriched CASO broth (Merck Chemicals (Pty) Ltd) for the bacteria and Sabouraud Dextrose 4% broth (Merck Chemicals (Pty) Ltd) for Candida ; in the 96-well microtiter-plate adding 100 μl of a McFarland Standard 1 inoculum of 48 hour old microorganisms grown at 37˚C.

Cell Counting:

Article Title: Effect of High-Pressure-Induced Ice I-to-Ice III Phase Transitions on Inactivation of Listeria innocua in Frozen Suspension
Article Snippet: .. One bead from a deep-frozen culture was transferred into standard I nutrient broth (Merck, Darmstadt, Germany), which was incubated at 30°C for 24 h. This broth was used to inoculate the final broth with a cell count of about 103 CFU/ml, which was incubated again at 30°C for 24 h to obtain bacteria in the stationary growth phase with a cell count of about 109 CFU/ml. .. This culture was harvested by centrifugation at 5,200 × g for 15 min at room temperature and resuspended in phosphate-buffered saline (PBS) to the same volume.

Stripping Membranes:

Article Title: Quantification of Endospore-Forming Firmicutes by Quantitative PCR with the Functional Gene spo0A
Article Snippet: The media used were standard nutrient broth (NB); DSM medium ( ); standard I nutrient broth (ST1; Merck Millipore, Darmstadt, Germany); PTYG medium composed of 0.25 g/liter peptone, 0.25 g/liter tryptone, 0.5 g/liter yeast extract, 0.5 g/liter glucose, 0.6 g/liter MgSO4 ·7H2 O, 0.07 g/liter CaCl2 ·2 H2 O (pH was adjusted with NaOH and H2 SO4 ); FeSO medium (described elsewhere [ ]); Difco2216*, corresponding to a modified version of marine broth (also described elsewhere [ ]) supplemented with 20 mM sodium pyruvate and 1 mg/liter of resazurin to verify anoxia. .. The incubation was at 37°C under anaerobic conditions attained by placing the plates in sealed plastic bags in an Anaerocult A mini apparatus and one strip of Anaerotest (Merck Millipore).

Expressing:

Article Title: An Asymmetric Model for Na+-translocating Glutaconyl-CoA Decarboxylases *
Article Snippet: .. For cloning, sequencing, and gene expression Escherichia coli strains DH5α (Invitrogen, Karlsruhe, Germany) ( ) and RosettaTM (DE3)pLysS (Novagen, Darmstadt, Germany) ( ) were grown in Luria-Bertani (LB) medium or standard I nutrient broth (Merck, Darmstadt, Germany) supplemented with the required antibiotics at 37 °C. .. The purification of the glutaconyl-CoA decarboxylase complex was carried out as previously described ( ).

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: After confirmation of the desired sequence, the genes were transferred from the donor vector to expression vector pASG IBA33 and further into E. coli BL21(GroEL). .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml).

Modification:

Article Title: Quantification of Endospore-Forming Firmicutes by Quantitative PCR with the Functional Gene spo0A
Article Snippet: .. The media used were standard nutrient broth (NB); DSM medium ( ); standard I nutrient broth (ST1; Merck Millipore, Darmstadt, Germany); PTYG medium composed of 0.25 g/liter peptone, 0.25 g/liter tryptone, 0.5 g/liter yeast extract, 0.5 g/liter glucose, 0.6 g/liter MgSO4 ·7H2 O, 0.07 g/liter CaCl2 ·2 H2 O (pH was adjusted with NaOH and H2 SO4 ); FeSO medium (described elsewhere [ ]); Difco2216*, corresponding to a modified version of marine broth (also described elsewhere [ ]) supplemented with 20 mM sodium pyruvate and 1 mg/liter of resazurin to verify anoxia. ..

Staining:

Article Title: Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide
Article Snippet: The media including the de Man–Rogosa–Sharpe (MRS) agar or broth (Merck, Darmstadt, Germany) were used. .. The isolated colonies were tested by microscopic examination with gram stain and catalase production.

Transformation Assay:

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: The PCR product was cloned into the p- ENTRY vector and introduced into Escherichia coli DH5α by chemical transformation. .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml).

Recombinant:

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml). ..

other:

Article Title: Biological Activities of Heteropyxis natalensis Against Micro-Organisms Involved in Oral Infections
Article Snippet: Inoculum was prepared by suspending microbial test organisms in enriched CASO broth [Merck Chemicals (Pty) Ltd.] for the bacteria and Sabouraud Dextrose 4% broth [Merck Chemicals (Pty) Ltd.] for Candida until turbidity was found to be compatible with McFarland Standard 1 [Merck Chemicals (Pty) Ltd., Wadeville, South Africa] ( ).

Article Title: Talin-Null Cells of Dictyostelium Are Strongly Defective in Adhesion to Particle and Substrate Surfaces and Slightly Impaired in Cytokinesis
Article Snippet: To achieve aggregation competence, AX2 cells were starved for 6 h, and talin-null cells were starved for 8 h. For growth in bacterial suspensions, Escherichia coli B /r or Salmonella minnesota R595 were cultivated overnight in standard I nutrient broth (Merck, Darmstadt, Germany), washed, and adjusted to a density corresponding to 1010 E. coli B /r cells per ml.

Plasmid Preparation:

Article Title: Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans *
Article Snippet: After confirmation of the desired sequence, the genes were transferred from the donor vector to expression vector pASG IBA33 and further into E. coli BL21(GroEL). .. The recombinant cells were cultivated in 4 liters of Standard I nutrient broth (Merck, Darmstadt, Germany) containing ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml).

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  • 93
    Merck KGaA m17 broth
    The profiles of BLIS activity (◆) and cell concentration (●) of P. acidilactici kp10 in <t>M17</t> media. The error bars represent the standard deviations about the mean (n=3).
    M17 Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m17 broth/product/Merck KGaA
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m17 broth - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Merck KGaA brain heart infusion broth bhi
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> <t>BHI,</t> brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Brain Heart Infusion Broth Bhi, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brain heart infusion broth bhi/product/Merck KGaA
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    brain heart infusion broth bhi - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Merck KGaA todd hewitt broth cultures
    Killing of Bacillus spores by 100 µg/ml of glycerol monolaurate (GML) in <t>Todd-Hewitt</t> broth at 37°C through the 24-h test period.
    Todd Hewitt Broth Cultures, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/todd hewitt broth cultures/product/Merck KGaA
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    todd hewitt broth cultures - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    97
    Merck KGaA tryptic soy broth tsb
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Tryptic Soy Broth Tsb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tryptic soy broth tsb/product/Merck KGaA
    Average 97 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    tryptic soy broth tsb - by Bioz Stars, 2020-04
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    Image Search Results


    The profiles of BLIS activity (◆) and cell concentration (●) of P. acidilactici kp10 in M17 media. The error bars represent the standard deviations about the mean (n=3).

    Journal: BioMed Research International

    Article Title: Stability of Bacteriocin-Like Inhibitory Substance (BLIS) Produced by Pediococcus acidilactici kp10 at Different Extreme Conditions

    doi: 10.1155/2018/5973484

    Figure Lengend Snippet: The profiles of BLIS activity (◆) and cell concentration (●) of P. acidilactici kp10 in M17 media. The error bars represent the standard deviations about the mean (n=3).

    Article Snippet: Effects of Commercial Media on Growth of P. acidilactici kp10 and BLIS Production Four types of commercial basal medium, (i) tryptic soy broth (TSB), (ii) nutrient broth (NB), (iii) MRS broth, and (iv) M17 broth (MERCK, Germany), were preliminary tested for the growth of P. acidilactici kp10 and BLIS production as fermentation conditions described in .

    Techniques: Activity Assay, Concentration Assay

    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, Luria-Bertani; BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.

    Journal: PLoS ONE

    Article Title: A novel bacteriocin from Enterococcus faecalis 478 exhibits a potent activity against vancomycin-resistant enterococci

    doi: 10.1371/journal.pone.0186415

    Figure Lengend Snippet: Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, Luria-Bertani; BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.

    Article Snippet: Three growth media namely Luria-Bertani medium (LB), De Man Rogosa Sharpe medium (MRS) and brain heart infusion broth (BHI) (all were purchased from Merck, Darmstadt, Germany) were used for comparison.

    Techniques: Incubation

    Killing of Bacillus spores by 100 µg/ml of glycerol monolaurate (GML) in Todd-Hewitt broth at 37°C through the 24-h test period.

    Journal: mSphere

    Article Title: Glycerol Monolaurate (GML) and a Nonaqueous Five-Percent GML Gel Kill Bacillus and Clostridium Spores

    doi: 10.1128/mSphereDirect.00597-18

    Figure Lengend Snippet: Killing of Bacillus spores by 100 µg/ml of glycerol monolaurate (GML) in Todd-Hewitt broth at 37°C through the 24-h test period.

    Article Snippet: Samples of Todd-Hewitt broth cultures were also centrifuged (1,000 × g , 15 min) and sterilized by filtration (Millex-GS; Merck Millipore Ltd., Tullagreen, Carrigtwohill, County Cork, Ireland) (0.22 μm pore size).

    Techniques:

    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas brassicacearum MA250 pure culture in TSB, 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each

    Journal: BMC Microbiology

    Article Title: Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations

    doi: 10.1186/s12866-019-1432-8

    Figure Lengend Snippet: Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas brassicacearum MA250 pure culture in TSB, 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each

    Article Snippet: P. brassicacearum MA250 cells were cultured in tryptic soy broth (TSB) (105,459, Merck, Germany) with shaking at 210 rpm or on TSA (TSB + agar) (105,458, Merck, Germany) at 25 °C.

    Techniques:

    Isothermal microcalorimetry thermal power (μW) curves of microbes in different culturing volumes (3, 6, 12 and 18 ml). a Pseudomonas brassicacearum MA250 in TSB, 25 °C. b Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. c Clonostachys rosea IK726 in PDB, 25 °C. The figures for Pseudomonas and Bacillus represent the averages and standard deviations of three independent experiments with 3–6 replicates per treatment. The figure for C. rosea IK726 represents the averages and standard deviations of six replicates per treatment

    Journal: BMC Microbiology

    Article Title: Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations

    doi: 10.1186/s12866-019-1432-8

    Figure Lengend Snippet: Isothermal microcalorimetry thermal power (μW) curves of microbes in different culturing volumes (3, 6, 12 and 18 ml). a Pseudomonas brassicacearum MA250 in TSB, 25 °C. b Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. c Clonostachys rosea IK726 in PDB, 25 °C. The figures for Pseudomonas and Bacillus represent the averages and standard deviations of three independent experiments with 3–6 replicates per treatment. The figure for C. rosea IK726 represents the averages and standard deviations of six replicates per treatment

    Article Snippet: P. brassicacearum MA250 cells were cultured in tryptic soy broth (TSB) (105,459, Merck, Germany) with shaking at 210 rpm or on TSA (TSB + agar) (105,458, Merck, Germany) at 25 °C.

    Techniques: