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Difco broth
Broth, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/broth/product/Difco
Average 99 stars, based on 2 article reviews
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broth - by Bioz Stars, 2020-04
99/100 stars

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Conjugation Assay:

Article Title: Serine/Threonine Protein Phosphatase-Mediated Control of the Peptidoglycan Cross-Linking l,d-Transpeptidase Pathway in Enterococcus faecium
Article Snippet: All cultures were performed at 37°C in BHI agar or broth (Difco Laboratories). .. Derivatives of pJEH11 were introduced into strains of E. faecium by conjugation ( ) and electroporation ( ) as previously described, except that a field of 1 kV (2-mm gap) and a shunt resistance of 1,000 Ω were used for the latter technique.

Centrifugation:

Article Title: Morphology, Physiological Characteristics, and Complete Sequence of Marine Bacteriophage ?RIO-1 Infecting Pseudoalteromonas marina
Article Snippet: For thermal or pH stability measurements, phage at a starting concentration of 108 PFU/ml in marine broth (Difco) was held under the indicated conditions for 1 h before titers were determined. .. Briefly, the chemical solvent was mixed with phage at 109 PFU/ml at the indicated ratio, and the mixture was held at 25°C with shaking for 1 h. After centrifugation in the case of immiscible solvents, the organic solvent was removed by evaporation overnight at 4°C.

Selection:

Article Title: Haemophilus influenzae Forms Biofilms on Airway Epithelia
Article Snippet: All strains were reconstituted from frozen glycerol stock cultures and propagated on brain heart infusion (BHI) agar or broth (Difco, Detroit, MI) supplemented with 10 μg of hemin (Sigma Chemical Co., St. Louis, MO) per milliliter and 10 μg of nicotinamide-adenine nucleotide (Sigma) per milliliter at 37°C. .. For pRSM2211 plasmid selection, kanamycin (Sigma) 20 μg/ml was added to BHI broth or agar.

Article Title: Serine/Threonine Protein Phosphatase-Mediated Control of the Peptidoglycan Cross-Linking l,d-Transpeptidase Pathway in Enterococcus faecium
Article Snippet: Paragraph title: Bacterial strains, selection of mutants, and antibiotic susceptibility assay. ... All cultures were performed at 37°C in BHI agar or broth (Difco Laboratories).

Clone Assay:

Article Title: Identification, Characterization, and Variable Expression of a Naturally Occurring Inhibitor Protein of IS1106 Transposase in Clinical Isolates of Neisseriameningitidis
Article Snippet: All meningococcal strains were cultured on chocolate agar (Becton-Dickinson) or on GC agar or broth (Difco) supplemented with 1% (vol/vol) Polyvitox (Bio-Merieux) at 37°C in 5% CO2 . .. Escherichia coli strain DH5α [F− Φ80d lacZ ΔM15 endA1 recA1 hsdR17 supE44 thi-1 λ− gyrA96 Δ( lacZYA-argF ) U169 ] was used in cloning procedures.

Electroporation:

Article Title: Serine/Threonine Protein Phosphatase-Mediated Control of the Peptidoglycan Cross-Linking l,d-Transpeptidase Pathway in Enterococcus faecium
Article Snippet: All cultures were performed at 37°C in BHI agar or broth (Difco Laboratories). .. Derivatives of pJEH11 were introduced into strains of E. faecium by conjugation ( ) and electroporation ( ) as previously described, except that a field of 1 kV (2-mm gap) and a shunt resistance of 1,000 Ω were used for the latter technique.

Isolation:

Article Title: Antiresorptive Activity of Bacillus-Fermented Antler Extracts: Inhibition of Osteoclast Differentiation
Article Snippet: Bacteria and Culture Conditions Bacillus subtilis strain K-11 was isolated from soybeans by Professor Dong-Hyun Kim (College of Pharmacy, Kyung Hee University, Seoul, Korea). .. Bacteria were cultured in Tryptic Soy Agar or Broth (Difco, KS).

Article Title: Identification, Characterization, and Variable Expression of a Naturally Occurring Inhibitor Protein of IS1106 Transposase in Clinical Isolates of Neisseriameningitidis
Article Snippet: Two strains, BL9513 and BF9513, were isolated, respectively, from the cerebrospinal fluid and the nasopharynx of a single sick subject in France. .. All meningococcal strains were cultured on chocolate agar (Becton-Dickinson) or on GC agar or broth (Difco) supplemented with 1% (vol/vol) Polyvitox (Bio-Merieux) at 37°C in 5% CO2 .

Cell Culture:

Article Title: Development and Characterization of a Novel Live Attenuated Vaccine Against Enteric Septicemia of Catfish
Article Snippet: .. E. coli strains CC118λ pir and SM10λ pir were cultured on Luria-Bertani (LB) agar or broth (Difco, Sparks, MD, United States) and incubated at 37°C throughout the study. .. When required, media were supplemented with the following antibiotics and reagents: ampicillin (amp: 100 mg/ml), colistin sulfate (col: 12.5 mg/ml), sucrose (5%), and mannitol (0.35%) (Sigma-Aldrich, St. Louis, MO, United States).

Article Title: Antiresorptive Activity of Bacillus-Fermented Antler Extracts: Inhibition of Osteoclast Differentiation
Article Snippet: .. Bacteria were cultured in Tryptic Soy Agar or Broth (Difco, KS). ..

Article Title: Identification, Characterization, and Variable Expression of a Naturally Occurring Inhibitor Protein of IS1106 Transposase in Clinical Isolates of Neisseriameningitidis
Article Snippet: .. All meningococcal strains were cultured on chocolate agar (Becton-Dickinson) or on GC agar or broth (Difco) supplemented with 1% (vol/vol) Polyvitox (Bio-Merieux) at 37°C in 5% CO2 . .. Escherichia coli strain DH5α [F− Φ80d lacZ ΔM15 endA1 recA1 hsdR17 supE44 thi-1 λ− gyrA96 Δ( lacZYA-argF ) U169 ] was used in cloning procedures.

Evaporation:

Article Title: Bactericidal Effect of Different Anti-Microbial Agents on Fusobacterium Nucleatum Biofilm
Article Snippet: Turmeric solution was obtained from the curcuma longa rhizomes by maceration method and rota evaporation was used to concentrate the extract. .. These slides were further dipped in Petri dishes containing neutralizing broth (Difco Laboratories, Michigan) supplemented with 0.5% Tween 20 (HiMedia Laboratories, India) to stop the antibacterial reaction of the irrigants.

Article Title: Morphology, Physiological Characteristics, and Complete Sequence of Marine Bacteriophage ?RIO-1 Infecting Pseudoalteromonas marina
Article Snippet: For thermal or pH stability measurements, phage at a starting concentration of 108 PFU/ml in marine broth (Difco) was held under the indicated conditions for 1 h before titers were determined. .. Briefly, the chemical solvent was mixed with phage at 109 PFU/ml at the indicated ratio, and the mixture was held at 25°C with shaking for 1 h. After centrifugation in the case of immiscible solvents, the organic solvent was removed by evaporation overnight at 4°C.

Drug Susceptibility Assay:

Article Title: Serine/Threonine Protein Phosphatase-Mediated Control of the Peptidoglycan Cross-Linking l,d-Transpeptidase Pathway in Enterococcus faecium
Article Snippet: Paragraph title: Bacterial strains, selection of mutants, and antibiotic susceptibility assay. ... All cultures were performed at 37°C in BHI agar or broth (Difco Laboratories).

Incubation:

Article Title: Identification of Nonlipophilic Corynebacteria Isolated from Dairy Cows with Mastitis
Article Snippet: .. Nitrate broth (Difco) in Durham tubes was incubated for 2 days. .. Amylase activity was recorded after 1 or 2 days on spot-inoculated Columbia agar (Biolife, Milan, Italy), which contains 1 g of starch/liter, on the same medium with a supplement of 0.5 g of soluble starch/liter, and additionally with the rapid amylase test (Biolife).

Article Title: Development and Characterization of a Novel Live Attenuated Vaccine Against Enteric Septicemia of Catfish
Article Snippet: .. E. coli strains CC118λ pir and SM10λ pir were cultured on Luria-Bertani (LB) agar or broth (Difco, Sparks, MD, United States) and incubated at 37°C throughout the study. .. When required, media were supplemented with the following antibiotics and reagents: ampicillin (amp: 100 mg/ml), colistin sulfate (col: 12.5 mg/ml), sucrose (5%), and mannitol (0.35%) (Sigma-Aldrich, St. Louis, MO, United States).

Article Title: Isolation and Characterization of Carnocyclin A, a Novel Circular Bacteriocin Produced by Carnobacterium maltaromaticum UAL307
Article Snippet: C. maltaromaticum UAL307 was grown at 25°C on all-purpose Tween (APT) agar or broth (Difco; Becton Dickinson Microbiology Systems, Sparks, MD). .. Gram-positive strains were incubated at 25°C, except for C. maltaromaticum UAL26, which was grown at 16°C.

Article Title: Response of Listeria monocytogenes to Disinfection Stress at the Single-Cell and Population Levels as Monitored by Intracellular pH Measurements and Viable-Cell Counts ▿
Article Snippet: Also, to determine the number of viable L. monocytogenes cells during disinfection, 40 μl of cell suspension was transferred to 160 μl Dey-Engly (DE) neutralizing broth (281910; Difco, BD, Sparks, France) every 5 min for 20 min. .. Bacterial numbers were determined by surface plating on BHI agar plates that were incubated at 37°C for 2 days.

Article Title: Bactericidal Effect of Different Anti-Microbial Agents on Fusobacterium Nucleatum Biofilm
Article Snippet: These slides were further dipped in Petri dishes containing neutralizing broth (Difco Laboratories, Michigan) supplemented with 0.5% Tween 20 (HiMedia Laboratories, India) to stop the antibacterial reaction of the irrigants. .. The scraped biofilms were inoculated on brain heart infusion agar plates and incubated at 37°C for 96 hours in an anaerobic jar.

other:

Article Title: Phenotypic Switching in Cryptococcus neoformans Results in Changes in Cellular Morphology and Glucuronoxylomannan Structure
Article Snippet: The media used included Sabouraud dextrose agar (SDA) and broth (SD) (Difco Laboratories, Detroit, Mich.), chemically defined media and agar (15 mM glucose, 10 mM MgSO4 , 29.4 mM KH2 PO4 , 3 μM thiamine), and SDA plates supplemented with 1 μM l -dopa (Sigma, Cleveland, Ohio).

Article Title: Sulfites inhibit the growth of four species of beneficial gut bacteria at concentrations regarded as safe for food
Article Snippet: The Lactobacillus species were grown and maintained on MRS agar or in MRS Broth (Difco) and S . thermophilus was grown and maintained on Brain Heart Infusion (BHI) agar or broth (Difco) at 36°C and under 5% CO2 .

Activity Assay:

Article Title: Identification of Nonlipophilic Corynebacteria Isolated from Dairy Cows with Mastitis
Article Snippet: Nitrate broth (Difco) in Durham tubes was incubated for 2 days. .. Amylase activity was recorded after 1 or 2 days on spot-inoculated Columbia agar (Biolife, Milan, Italy), which contains 1 g of starch/liter, on the same medium with a supplement of 0.5 g of soluble starch/liter, and additionally with the rapid amylase test (Biolife).

Plasmid Preparation:

Article Title: Haemophilus influenzae Forms Biofilms on Airway Epithelia
Article Snippet: We also studied NTHi strains 2019, 2019siaA , 2019siaB , and 2019wecA ( ) in our NTHi–epithelial coculture model. Plasmid pRSM2211 expressing green fluorescent protein (GFP) mut3 was kindly provided by Lauren Bakaletz ( ) and was electroporated into NTHi strain 2019 and NTHi strain 2019wecA by methods previously described ( ). .. All strains were reconstituted from frozen glycerol stock cultures and propagated on brain heart infusion (BHI) agar or broth (Difco, Detroit, MI) supplemented with 10 μg of hemin (Sigma Chemical Co., St. Louis, MO) per milliliter and 10 μg of nicotinamide-adenine nucleotide (Sigma) per milliliter at 37°C.

Expressing:

Article Title: Haemophilus influenzae Forms Biofilms on Airway Epithelia
Article Snippet: We also studied NTHi strains 2019, 2019siaA , 2019siaB , and 2019wecA ( ) in our NTHi–epithelial coculture model. Plasmid pRSM2211 expressing green fluorescent protein (GFP) mut3 was kindly provided by Lauren Bakaletz ( ) and was electroporated into NTHi strain 2019 and NTHi strain 2019wecA by methods previously described ( ). .. All strains were reconstituted from frozen glycerol stock cultures and propagated on brain heart infusion (BHI) agar or broth (Difco, Detroit, MI) supplemented with 10 μg of hemin (Sigma Chemical Co., St. Louis, MO) per milliliter and 10 μg of nicotinamide-adenine nucleotide (Sigma) per milliliter at 37°C.

Low Protein Binding:

Article Title: In-Depth Analysis of Exoproteomes from Marine Bacteria by Shotgun Liquid Chromatography-Tandem Mass Spectrometry: the Ruegeria pomeroyi DSS-3 Case-Study
Article Snippet: Exoproteome samples Three flasks containing 40 mL of marine broth (Difco) were inoculated with Ruegeria pomeroyi DSS-3 cells (DSM15171T) previously grown on agar plates. .. Supernatants were carefully filtered through two low protein-binding filters of 0.45 μm (Millex-HV) and then 0.22 μm (Millex-GV) diameter pore (Millipore) to eliminate any remaining cells.

Concentration Assay:

Article Title: Morphology, Physiological Characteristics, and Complete Sequence of Marine Bacteriophage ?RIO-1 Infecting Pseudoalteromonas marina
Article Snippet: .. For thermal or pH stability measurements, phage at a starting concentration of 108 PFU/ml in marine broth (Difco) was held under the indicated conditions for 1 h before titers were determined. ..

Recombinant:

Article Title: Identification, Characterization, and Variable Expression of a Naturally Occurring Inhibitor Protein of IS1106 Transposase in Clinical Isolates of Neisseriameningitidis
Article Snippet: All meningococcal strains were cultured on chocolate agar (Becton-Dickinson) or on GC agar or broth (Difco) supplemented with 1% (vol/vol) Polyvitox (Bio-Merieux) at 37°C in 5% CO2 . .. Strain BL21 λDE3 (F− ompT rB − mB − ) was used to overexpress recombinant proteins ( ).

Adsorption:

Article Title: Morphology, Physiological Characteristics, and Complete Sequence of Marine Bacteriophage ?RIO-1 Infecting Pseudoalteromonas marina
Article Snippet: For thermal or pH stability measurements, phage at a starting concentration of 108 PFU/ml in marine broth (Difco) was held under the indicated conditions for 1 h before titers were determined. .. Burst size, latent time, and adsorption rates were determined as described previously ( ).

Derivative Assay:

Article Title: Serine/Threonine Protein Phosphatase-Mediated Control of the Peptidoglycan Cross-Linking l,d-Transpeptidase Pathway in Enterococcus faecium
Article Snippet: All cultures were performed at 37°C in BHI agar or broth (Difco Laboratories). .. Mutants M1 and M512 were derived from E. faecium D344S by serial selection on medium containing increasing concentrations of ampicillin ( , ).

Micro-arrays for Mass Spectrometry:

Article Title: Consumption of Tropospheric Levels of Methyl Bromide by C1 Compound-Utilizing Bacteria and Comparison to Saturation Kinetics
Article Snippet: .. It was grown on marine broth (Difco 2216) either with or without MeBr or on a mineral salts medium (MAMS) supplied with MeBr as the sole carbon and energy source. .. The MAMS medium was adapted from that of Thompson et al. ( ) and contained (in grams per liter): NaCl, 16; (NH4 )2 SO4, 1.0; MgSO4 · 7H2 O, 1.0; CaCl2 · 2H2 O, 0.2; FeSO4 · 7H2 O, 0.002; Na2 MoO4 · 2H2 O, 0.002; Na2 WO4, 0.003; KH2 PO4, 0.36; K2 HPO4, 2.34; and 1.0 ml of SL-10 trace metals ( ).

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  • 94
    Difco tryptone soy broth tsb
    Growth of S. aureus SH1000 and the arcR deletion mutant under anaerobic conditions. Cultures were plated onto <t>TSB</t> solid medium supplemented with 25 mM glucose or 50 mM arginine or both as the main energy source and incubated for 72 h at <t>37°C.</t> wt, wild type.
    Tryptone Soy Broth Tsb, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tryptone soy broth tsb/product/Difco
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tryptone soy broth tsb - by Bioz Stars, 2020-04
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    85
    Difco broth culture filtrate bcf
    Cell-cycle inhibition in HDF cells. BrdU incorporation in HDF cells incubated with: (A) <t>UBF</t> and <t>BCF</t> treated with the L-asparaginase inhibitor 5-diazo-4-oxo-L-norvaline (DONV, 20 mM) or the GGT inhibitor acivicin (ACI, 5 mM), (B) UBF and BCF derived from different H. pylori strains, (C) different concentrations of recombinant L-asparaginase. Results are expressed as a mean ± SD from 5 independent experiments. * P≤0.05, ** P≤0.01.
    Broth Culture Filtrate Bcf, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/broth culture filtrate bcf/product/Difco
    Average 85 stars, based on 1 article reviews
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    92
    Difco 2216e broth
    Expression of the recombinant ErmC protein in SM20429 and E. coli with the constructed vector pWD-ermC . (A) SDS-PAGE analysis of the expressed ErmC protein in the total-cell lysates of recombinant SM20429 and E. coli . Lane 1, SM20429 harboring pWD2, used as a negative control; Lane2, SM20429 harboring pWD-ermC; Lane3, E. coli DH5α harboring pWD-ermC, used as a positive control. The SDS-PAGE gel was stained with Coomassie blue. Arrow denotes the band of the expressed ErmC. (B) Analysis of the ErmC-resistance of the transformed SM20429. 1, pWD-ermC-harboring SM20429 inoculated in <t>2216E</t> medium supplemented with 30 μg/ml EM; 2, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 3, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml EM; 4, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml.
    2216e Broth, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2216e broth/product/Difco
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    95
    Difco todd hewitt broth
    Effect of Dectin-2 deficiency on IL-12p40 production by BD-DCs upon stimulation with S. pneumoniae . BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with live S. pneumoniae ( a ); lysates from S. pneumoniae or sham-operated PBS (Sham) ( b ); S. pneumoniae culture supernatant or <t>Todd-Hewitt</t> broth (THB) ( c ); or LPS (1 μg/ml), CpG (1 μg/ml) or mannan (3 mg/ml) for 24 h. IL-12p40 concentrations in the culture supernatants were measured using ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. MOI, multiplicity of infection. *, p
    Todd Hewitt Broth, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 55 article reviews
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    Growth of S. aureus SH1000 and the arcR deletion mutant under anaerobic conditions. Cultures were plated onto TSB solid medium supplemented with 25 mM glucose or 50 mM arginine or both as the main energy source and incubated for 72 h at 37°C. wt, wild type.

    Journal: Journal of Bacteriology

    Article Title: Staphylococcus aureus ArcR Controls Expression of the Arginine Deiminase Operon ▿ ArcR Controls Expression of the Arginine Deiminase Operon ▿ †

    doi: 10.1128/JB.00592-07

    Figure Lengend Snippet: Growth of S. aureus SH1000 and the arcR deletion mutant under anaerobic conditions. Cultures were plated onto TSB solid medium supplemented with 25 mM glucose or 50 mM arginine or both as the main energy source and incubated for 72 h at 37°C. wt, wild type.

    Article Snippet: S. aureus strains were grown at 37°C in tryptone soy broth (TSB) (Difco) and brain heart infusion broth (Difco) supplemented with erythromycin (12 μg ml−1 ) and kanamycin (200 μg ml−1 ) where appropriate.

    Techniques: Mutagenesis, Incubation

    Cell-cycle inhibition in HDF cells. BrdU incorporation in HDF cells incubated with: (A) UBF and BCF treated with the L-asparaginase inhibitor 5-diazo-4-oxo-L-norvaline (DONV, 20 mM) or the GGT inhibitor acivicin (ACI, 5 mM), (B) UBF and BCF derived from different H. pylori strains, (C) different concentrations of recombinant L-asparaginase. Results are expressed as a mean ± SD from 5 independent experiments. * P≤0.05, ** P≤0.01.

    Journal: PLoS ONE

    Article Title: Cell-Cycle Inhibition by Helicobacter pylori L-Asparaginase

    doi: 10.1371/journal.pone.0013892

    Figure Lengend Snippet: Cell-cycle inhibition in HDF cells. BrdU incorporation in HDF cells incubated with: (A) UBF and BCF treated with the L-asparaginase inhibitor 5-diazo-4-oxo-L-norvaline (DONV, 20 mM) or the GGT inhibitor acivicin (ACI, 5 mM), (B) UBF and BCF derived from different H. pylori strains, (C) different concentrations of recombinant L-asparaginase. Results are expressed as a mean ± SD from 5 independent experiments. * P≤0.05, ** P≤0.01.

    Article Snippet: For routine culture, the bacterial broth culture filtrate (BCF) and Uninoculated Broth culture Filtrate (UBF) were produced in brucella broth (Difco, Detroit, MI) supplemented with 5% inactivated foetal bovine serum (FBS, Invitrogen, Milan, Italy), Skirrow and Vitox (Oxoid, Basing-stoke, UK) as described .

    Techniques: Inhibition, BrdU Incorporation Assay, Incubation, Derivative Assay, Recombinant

    Expression of the recombinant ErmC protein in SM20429 and E. coli with the constructed vector pWD-ermC . (A) SDS-PAGE analysis of the expressed ErmC protein in the total-cell lysates of recombinant SM20429 and E. coli . Lane 1, SM20429 harboring pWD2, used as a negative control; Lane2, SM20429 harboring pWD-ermC; Lane3, E. coli DH5α harboring pWD-ermC, used as a positive control. The SDS-PAGE gel was stained with Coomassie blue. Arrow denotes the band of the expressed ErmC. (B) Analysis of the ErmC-resistance of the transformed SM20429. 1, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 2, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 3, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml EM; 4, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml.

    Journal: Microbial Cell Factories

    Article Title: Characterization of a cryptic plasmid pSM429 and its application for heterologous expression in psychrophilic Pseudoalteromonas

    doi: 10.1186/1475-2859-10-30

    Figure Lengend Snippet: Expression of the recombinant ErmC protein in SM20429 and E. coli with the constructed vector pWD-ermC . (A) SDS-PAGE analysis of the expressed ErmC protein in the total-cell lysates of recombinant SM20429 and E. coli . Lane 1, SM20429 harboring pWD2, used as a negative control; Lane2, SM20429 harboring pWD-ermC; Lane3, E. coli DH5α harboring pWD-ermC, used as a positive control. The SDS-PAGE gel was stained with Coomassie blue. Arrow denotes the band of the expressed ErmC. (B) Analysis of the ErmC-resistance of the transformed SM20429. 1, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 2, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 3, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml EM; 4, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml.

    Article Snippet: Curing of pSM429 from BSi20429 BSi20429 was grown for at least 8 subcultures (approximately 160 generations) in 2216E broth containing 0.07% SDS.

    Techniques: Expressing, Recombinant, Construct, Plasmid Preparation, SDS Page, Negative Control, Positive Control, Staining, Transformation Assay

    Effect of Dectin-2 deficiency on IL-12p40 production by BD-DCs upon stimulation with S. pneumoniae . BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with live S. pneumoniae ( a ); lysates from S. pneumoniae or sham-operated PBS (Sham) ( b ); S. pneumoniae culture supernatant or Todd-Hewitt broth (THB) ( c ); or LPS (1 μg/ml), CpG (1 μg/ml) or mannan (3 mg/ml) for 24 h. IL-12p40 concentrations in the culture supernatants were measured using ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. MOI, multiplicity of infection. *, p

    Journal: BMC Immunology

    Article Title: Dectin-2-dependent host defense in mice infected with serotype 3 Streptococcus pneumoniae

    doi: 10.1186/s12865-015-0139-3

    Figure Lengend Snippet: Effect of Dectin-2 deficiency on IL-12p40 production by BD-DCs upon stimulation with S. pneumoniae . BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with live S. pneumoniae ( a ); lysates from S. pneumoniae or sham-operated PBS (Sham) ( b ); S. pneumoniae culture supernatant or Todd-Hewitt broth (THB) ( c ); or LPS (1 μg/ml), CpG (1 μg/ml) or mannan (3 mg/ml) for 24 h. IL-12p40 concentrations in the culture supernatants were measured using ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. MOI, multiplicity of infection. *, p

    Article Snippet: Todd-Hewitt broth incubated without S. pneumoniae was used as a control.

    Techniques: Mouse Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Infection