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Becton Dickinson broth
Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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broth - by Bioz Stars, 2020-04
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Diagnostic Assay:

Article Title: Influence of oxyR on Growth, Biofilm Formation, and Mobility of Vibrio parahaemolyticus
Article Snippet: .. It was cultured at 37°C on tryptic soy agar or broth (Becton-Dickinson Diagnostic Systems, Sparks, MD, USA) without glucose and phosphate (agar and broth herein abbreviated TSANG and TSBNG, respectively) and was supplemented with 3% sodium chloride, and the pH was adjusted to 7.3. .. The broth cultures were incubated at 37°C statically or shaken at 160 rpm.

Clone Assay:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: E. coli strains Top10 was used as a recipient for cloning, mutagenesis, and plasmid propagation. .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Amplification:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: PCR amplification using Expand High Fidelity PCR system (Roche Applied Science) was performed with Gene Amp PCR system 9700 (AB Applied Biosystems, Carlsbad, CA). .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Synthesized:

Article Title: Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain
Article Snippet: Paragraph title: Minimum inhibitory concentrations (MICs) and minimum biocidal concentrations (MBCs) of AgNPs synthesized from S. xinghaiensis OF1 strain against pathogenic bacteria and yeasts ... Briefly, the microbial inoculum (5 × 105 c.f.u. ml−1 ) was diluted with appropriate broth (1:1000) and 100 µl was then spread on the surface of Tripticase Soy Agar (TSA, Becton Dickinson) or Sabouraud Dextrose Agar (SDA, Becton Dickinson).

Quantitative RT-PCR:

Article Title: Analysis of Drosophila STING Reveals an Evolutionarily Conserved Antimicrobial Function
Article Snippet: S2 cells are negative for the presence of Flock House virus by qRT-PCR using primers for the gene coding for B2 ( ). .. Listeria monocytogenes serotype 1/2a (strain 10403S, ATCC) was grown in Difco Brain-heart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30°C.

Incubation:

Article Title: Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain
Article Snippet: Briefly, the microbial inoculum (5 × 105 c.f.u. ml−1 ) was diluted with appropriate broth (1:1000) and 100 µl was then spread on the surface of Tripticase Soy Agar (TSA, Becton Dickinson) or Sabouraud Dextrose Agar (SDA, Becton Dickinson). .. After incubation, the presence of approximately 50 colonies indicate an inoculum density of 5 × 105 c.f.u. ml−1 .

Article Title: Fenretinide inhibited de novo ceramide synthesis and proinflammatory cytokines induced by Aggregatibacter actinomycetemcomitans
Article Snippet: .. A.a frozen stock solution was streaked on anaerobe 5% sheep blood agar plates (BD Biosciences, Sparks, MD) and incubated in a 37°C incubator with 10% CO2 for 24 h. A . a colonies were cultured in NIH thioglycollate broth (BD Biosciences) overnight at 37°C with 10% CO2 for 24 h. Bacteria were centrifuged and fixed with 4% paraformaldehyde for 1 h. Finally, the bacteria were washed with PBS five times, centrifuged, and resuspended in PBS. ..

Article Title: Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli
Article Snippet: .. For general growth, bacterial strains were grown in either Miller Difco Luria–Bertani (LB) broth (Becton, Dickinson and Co., Sparks, MD, USA) or on LB agar; LB broth with select agar (Sigma-Aldrich) at 15 g L−1 and incubated at 37 °C. ..

Article Title: Influence of oxyR on Growth, Biofilm Formation, and Mobility of Vibrio parahaemolyticus
Article Snippet: It was cultured at 37°C on tryptic soy agar or broth (Becton-Dickinson Diagnostic Systems, Sparks, MD, USA) without glucose and phosphate (agar and broth herein abbreviated TSANG and TSBNG, respectively) and was supplemented with 3% sodium chloride, and the pH was adjusted to 7.3. .. The broth cultures were incubated at 37°C statically or shaken at 160 rpm.

Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
Article Snippet: Analysis of biofilm formation Overnight cultures in Difco Luria-Bertani (LB) broth (Lennox, Becton Dickinson, Franklin Lakes, USA) were used to inoculate (at 0.1 %) fresh minimal salt (MS) medium [ ] on agitation to an optical density (OD620 ) of approximately 0.05. .. After an additional 18 h of incubation under static conditions at 37 °C, biofilm formation was quantified by the crystal violet method as previously described [ ].

Cell Culture:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions. ..

Article Title: Fenretinide inhibited de novo ceramide synthesis and proinflammatory cytokines induced by Aggregatibacter actinomycetemcomitans
Article Snippet: .. A.a frozen stock solution was streaked on anaerobe 5% sheep blood agar plates (BD Biosciences, Sparks, MD) and incubated in a 37°C incubator with 10% CO2 for 24 h. A . a colonies were cultured in NIH thioglycollate broth (BD Biosciences) overnight at 37°C with 10% CO2 for 24 h. Bacteria were centrifuged and fixed with 4% paraformaldehyde for 1 h. Finally, the bacteria were washed with PBS five times, centrifuged, and resuspended in PBS. ..

Article Title: Capsular Polysaccharide Surrounds Smooth and Rugose Types of Salmonella enterica serovar Typhimurium DT104 † serovar Typhimurium DT104 † ‡
Article Snippet: .. Serovar Typhimurium DT104 and E. coli were cultured on Trypticase soy agar and Luria-Bertani (LB) agar and broth (Becton Dickinson, Franklin Lakes, NJ) at 25°C and 37°C for the formation of rugose (Rv/25)- and smooth (Rv/37)-colony variant phenotypes, respectively ( ). .. A stable, smooth, spontaneous mutant of serovar Typhimurium DT104 (Stv) , which forms smooth colonies at both temperatures, was used as a negative control in all experiments.

Article Title: Inactivation of Cyclic Di-GMP Binding Protein TDE0214 Affects the Motility, Biofilm Formation, and Virulence of Treponema denticola
Article Snippet: .. E. coli strains were cultured in Difco Luria-Bertani (LB) broth (BD Biosciences, Sparks, MD) supplemented with 100 μg/ml of ampicillin. .. The entire open reading frame (ORF) of TDE0214 was PCR amplified with the primers P12 /P13 using Platinum pfx DNA polymerase (Invitrogen).

Article Title: Influence of oxyR on Growth, Biofilm Formation, and Mobility of Vibrio parahaemolyticus
Article Snippet: .. It was cultured at 37°C on tryptic soy agar or broth (Becton-Dickinson Diagnostic Systems, Sparks, MD, USA) without glucose and phosphate (agar and broth herein abbreviated TSANG and TSBNG, respectively) and was supplemented with 3% sodium chloride, and the pH was adjusted to 7.3. .. The broth cultures were incubated at 37°C statically or shaken at 160 rpm.

Article Title: A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa
Article Snippet: All marine strains used were routinely grown at 28 °C in marine broth (MB, BD Difco® , Franklin Lakes, NJ, USA). .. The human-related pathogen P. aeruginosa PAO1 was cultured in LB medium at 37 °C.

Article Title: Analysis of Drosophila STING Reveals an Evolutionarily Conserved Antimicrobial Function
Article Snippet: Paragraph title: Cell culture and pathogens ... Listeria monocytogenes serotype 1/2a (strain 10403S, ATCC) was grown in Difco Brain-heart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30°C.

Mass Spectrometry:

Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
Article Snippet: .. Analysis of biofilm formation Overnight cultures in Difco Luria-Bertani (LB) broth (Lennox, Becton Dickinson, Franklin Lakes, USA) were used to inoculate (at 0.1 %) fresh minimal salt (MS) medium [ ] on agitation to an optical density (OD620 ) of approximately 0.05. .. The bacteria were seeded into 96-well plastic plates (Nunc C96 Microwell plate, Nunc A/S, Roskilde, Denmark) and after 6 h at 37 °C exposed to sub-inhibitory concentrations (100 or 250 μM) of CORM-2 ([Ru(CO)3 Cl2 ]2 (Sigma-Aldrich, St. Louis, MO, USA) or left untreated.

Modification:

Article Title: Analysis of Drosophila STING Reveals an Evolutionarily Conserved Antimicrobial Function
Article Snippet: 293T cells and STING−/− MEFs ( ) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 4.5 g/L glucose, 110 mg/L sodium pyruvate, 4 mM L-glutamine, 10% FBS and 1 × antibiotic-antimycotic. .. Listeria monocytogenes serotype 1/2a (strain 10403S, ATCC) was grown in Difco Brain-heart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30°C.

Conjugation Assay:

Article Title: Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII
Article Snippet: Strains and plasmids Clostridium difficile 630 (Sebaihia et al ., ) and its erythromycin‐sensitive derivative 630Δerm (Hussain et al ., ) were grown under anaerobic conditions (80%N2 , 10% CO2 , 10% H2 ) in an anaerobic incubator (Don Whitley Scientific) on Brain Heart Infusion Supplemented (BHIS) plates or in broth [BHI supplemented with 5 g l−1 yeast extract (BD Bacto), and 1 g l−1 L‐cysteine]. .. E. coli strain NovaBlue (Merck) was used as a recipient for all recombinant plasmids, and strain CA434 (HB101 carrying R702) was used for conjugation of plasmids from E. coli into C. difficile .

Infection:

Article Title: Leishmanicidal Activity of Isoselenocyanate Derivatives
Article Snippet: To maintain their infectivity, Leishmania cells were isolated from infected BALB/c mouse spleen and parasites were maintained in culture for not more than five passages. .. Animals were inoculated with 2 ml sterile thioglycolate (3%) broth (BD Difco) prior to peritoneal cavity lavage with 5 ml of cold RPMI medium, and macrophages were removed by a syringe as previously described ( ).

Article Title: Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli
Article Snippet: We used 10 clinical, urinary-tract infection isolates of E. coli from the ECO-SENS collections , originating from countries across Europe between 2000 and 2008 (Table ). .. For general growth, bacterial strains were grown in either Miller Difco Luria–Bertani (LB) broth (Becton, Dickinson and Co., Sparks, MD, USA) or on LB agar; LB broth with select agar (Sigma-Aldrich) at 15 g L−1 and incubated at 37 °C.

Purification:

Article Title: Analysis of Drosophila STING Reveals an Evolutionarily Conserved Antimicrobial Function
Article Snippet: Invertebrate iridescent virus 6 (IIV6) was kindly provided by Luís Teixeira and purified as previously described ( ). .. Listeria monocytogenes serotype 1/2a (strain 10403S, ATCC) was grown in Difco Brain-heart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30°C.

DNA Sequencing:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: DNA sequencing was accomplished using the ABI prism Genetic Analyzer 3500 (Invitrogen, Carlsbad, CA). .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Polymerase Chain Reaction:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: PCR amplification using Expand High Fidelity PCR system (Roche Applied Science) was performed with Gene Amp PCR system 9700 (AB Applied Biosystems, Carlsbad, CA). .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Recombinant:

Article Title: Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII
Article Snippet: Strains and plasmids Clostridium difficile 630 (Sebaihia et al ., ) and its erythromycin‐sensitive derivative 630Δerm (Hussain et al ., ) were grown under anaerobic conditions (80%N2 , 10% CO2 , 10% H2 ) in an anaerobic incubator (Don Whitley Scientific) on Brain Heart Infusion Supplemented (BHIS) plates or in broth [BHI supplemented with 5 g l−1 yeast extract (BD Bacto), and 1 g l−1 L‐cysteine]. .. E. coli strain NovaBlue (Merck) was used as a recipient for all recombinant plasmids, and strain CA434 (HB101 carrying R702) was used for conjugation of plasmids from E. coli into C. difficile .

Article Title: Inactivation of Cyclic Di-GMP Binding Protein TDE0214 Affects the Motility, Biofilm Formation, and Virulence of Treponema denticola
Article Snippet: Escherichia coli TOP10 strain (Invitrogen, Carlsbad, CA) was used for routine plasmid constructions and preparations, an E. coli dam − / dcm − strain (New England BioLabs, Ipswich, MA) was used to prepare unmethylated plasmids, and the E. coli BL21 Star (DE3) strain (Invitrogen) was used for preparing recombinant proteins. .. E. coli strains were cultured in Difco Luria-Bertani (LB) broth (BD Biosciences, Sparks, MD) supplemented with 100 μg/ml of ampicillin.

MTT Assay:

Article Title: Leishmanicidal Activity of Isoselenocyanate Derivatives
Article Snippet: Animals were inoculated with 2 ml sterile thioglycolate (3%) broth (BD Difco) prior to peritoneal cavity lavage with 5 ml of cold RPMI medium, and macrophages were removed by a syringe as previously described ( ). .. An MTT test (Sigma, St. Louis, MO) was performed to determine the cytotoxicity of selected compounds in murine macrophages.

Mutagenesis:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: E. coli strains Top10 was used as a recipient for cloning, mutagenesis, and plasmid propagation. .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Article Title: Capsular Polysaccharide Surrounds Smooth and Rugose Types of Salmonella enterica serovar Typhimurium DT104 † serovar Typhimurium DT104 † ‡
Article Snippet: Serovar Typhimurium DT104 and E. coli were cultured on Trypticase soy agar and Luria-Bertani (LB) agar and broth (Becton Dickinson, Franklin Lakes, NJ) at 25°C and 37°C for the formation of rugose (Rv/25)- and smooth (Rv/37)-colony variant phenotypes, respectively ( ). .. A stable, smooth, spontaneous mutant of serovar Typhimurium DT104 (Stv) , which forms smooth colonies at both temperatures, was used as a negative control in all experiments.

Article Title: Inactivation of Cyclic Di-GMP Binding Protein TDE0214 Affects the Motility, Biofilm Formation, and Virulence of Treponema denticola
Article Snippet: T. denticola ATCC 35405 (wild type) and its derivative mutant strains were grown in oral bacterial growth medium (OBGM) containing 10% heat-inactivated rabbit serum at 37°C in an AS-580 anaerobic chamber (Anaerobe Systems, Morgan Hill, CA) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen, as previously described ( , ). .. E. coli strains were cultured in Difco Luria-Bertani (LB) broth (BD Biosciences, Sparks, MD) supplemented with 100 μg/ml of ampicillin.

Isolation:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: Molecular techniques Bacterial genomic and plasmid DNA were isolated using a Roche High Pure PCR Template Preparation kit (Roche Applied Science, Indianapolis, IN) and QIAprep Spin Miniprep Kit (Qiagen, Valencia, CA), respectively, according to the manufacturer's recommendations. .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Article Title: Leishmanicidal Activity of Isoselenocyanate Derivatives
Article Snippet: To maintain their infectivity, Leishmania cells were isolated from infected BALB/c mouse spleen and parasites were maintained in culture for not more than five passages. .. Animals were inoculated with 2 ml sterile thioglycolate (3%) broth (BD Difco) prior to peritoneal cavity lavage with 5 ml of cold RPMI medium, and macrophages were removed by a syringe as previously described ( ).

Article Title: Influence of oxyR on Growth, Biofilm Formation, and Mobility of Vibrio parahaemolyticus
Article Snippet: V. parahaemolyticus strain KX-V231 (Kanagawa phenomenon positive, serotype O3:K6), which was isolated in Thailand from a clinical specimen, was used in this work ( ). .. It was cultured at 37°C on tryptic soy agar or broth (Becton-Dickinson Diagnostic Systems, Sparks, MD, USA) without glucose and phosphate (agar and broth herein abbreviated TSANG and TSBNG, respectively) and was supplemented with 3% sodium chloride, and the pH was adjusted to 7.3.

Article Title: Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater
Article Snippet: Strain G3 was isolated from seawater from the Gulf Coast of Alabama, and the others were from seawater from the Delaware Bay. .. All vibrios were enriched in Difco Luria-Bertani (LB) broth (Becton, Dickinson and Company, Sparks, MD) supplemented with 2% NaCl (3% NaCl total), whereas non- Vibrio bacteria were grown in LB broth which contained 1% NaCl.

Titration:

Article Title: Analysis of Drosophila STING Reveals an Evolutionarily Conserved Antimicrobial Function
Article Snippet: Titration was performed on S2 cells as previously described ( ). .. Listeria monocytogenes serotype 1/2a (strain 10403S, ATCC) was grown in Difco Brain-heart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30°C.

Sequencing:

Article Title: Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli
Article Snippet: Subsequent analysis based on whole-genome sequencing discovered two changes to previously reported sequence types and the presence of plasmid replicons in three strains (Supplementary Table ). .. For general growth, bacterial strains were grown in either Miller Difco Luria–Bertani (LB) broth (Becton, Dickinson and Co., Sparks, MD, USA) or on LB agar; LB broth with select agar (Sigma-Aldrich) at 15 g L−1 and incubated at 37 °C.

Mouse Assay:

Article Title: Leishmanicidal Activity of Isoselenocyanate Derivatives
Article Snippet: Murine peritoneal macrophages from 4- to 6-week-old BALB/c mice were used for the study. .. Animals were inoculated with 2 ml sterile thioglycolate (3%) broth (BD Difco) prior to peritoneal cavity lavage with 5 ml of cold RPMI medium, and macrophages were removed by a syringe as previously described ( ).

Capillary Electrochromatography:

Article Title: Association of Amino Acid Substitutions in Penicillin-Binding Protein 3 with ?-Lactam Resistance in ?-Lactamase-Negative Ampicillin-Resistant Haemophilus influenzae
Article Snippet: The medium used for growth and determination of the MIC was Mueller-Hinton agar or broth (Becton Dickinson and Company, Sparks, Md.) supplemented with 2% defibrinated and heat-treated horse blood, 0.5% yeast extract, and 15 μg of β-NAD+ per ml. .. The β-lactams used in the study were AMP and cefditoren (CDN; Meiji Seika Kaisha, Ltd., Tokyo, Japan), cefotaxime (CTX; Nippon Hoechst Marion Roussel Ltd., Tokyo, Japan), ceftriaxone (CRO; Nippon Roch Ltd., Tokyo, Japan), cefaclor (CEC) and cefcapene (CPN) (Shionogi & Co., Ltd., Osaka, Japan), cefdinir (CDR; Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan), cefpodoxime (CPD; Sankyo Co., Ltd., Tokyo, Japan), faropenem (FRM; Suntory Ltd., Osaka, Japan), and meropenem (MEM; Sumitomo Pharmaceuticals Co., Ltd., Tokyo, Japan).

Plasmid Preparation:

Article Title: Campylobacter jejuni Type VI Secretion System: Roles in Adaptation to Deoxycholic Acid, Host Cell Adherence, Invasion, and In Vivo Colonization
Article Snippet: E. coli strains Top10 was used as a recipient for cloning, mutagenesis, and plasmid propagation. .. E. coli was cultured in Luria-Bertani (LB) agar or broth (BD) with continuous shaking (250 rpm) at 37°C under aerobic conditions.

Article Title: Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli
Article Snippet: Subsequent analysis based on whole-genome sequencing discovered two changes to previously reported sequence types and the presence of plasmid replicons in three strains (Supplementary Table ). .. For general growth, bacterial strains were grown in either Miller Difco Luria–Bertani (LB) broth (Becton, Dickinson and Co., Sparks, MD, USA) or on LB agar; LB broth with select agar (Sigma-Aldrich) at 15 g L−1 and incubated at 37 °C.

Article Title: Inactivation of Cyclic Di-GMP Binding Protein TDE0214 Affects the Motility, Biofilm Formation, and Virulence of Treponema denticola
Article Snippet: Escherichia coli TOP10 strain (Invitrogen, Carlsbad, CA) was used for routine plasmid constructions and preparations, an E. coli dam − / dcm − strain (New England BioLabs, Ipswich, MA) was used to prepare unmethylated plasmids, and the E. coli BL21 Star (DE3) strain (Invitrogen) was used for preparing recombinant proteins. .. E. coli strains were cultured in Difco Luria-Bertani (LB) broth (BD Biosciences, Sparks, MD) supplemented with 100 μg/ml of ampicillin.

Negative Control:

Article Title: Capsular Polysaccharide Surrounds Smooth and Rugose Types of Salmonella enterica serovar Typhimurium DT104 † serovar Typhimurium DT104 † ‡
Article Snippet: Serovar Typhimurium DT104 and E. coli were cultured on Trypticase soy agar and Luria-Bertani (LB) agar and broth (Becton Dickinson, Franklin Lakes, NJ) at 25°C and 37°C for the formation of rugose (Rv/25)- and smooth (Rv/37)-colony variant phenotypes, respectively ( ). .. A stable, smooth, spontaneous mutant of serovar Typhimurium DT104 (Stv) , which forms smooth colonies at both temperatures, was used as a negative control in all experiments.

Selection:

Article Title: Characteristics of Clinical Shiga Toxin-Producing Escherichia coli Isolated from British Columbia
Article Snippet: Paragraph title: 2.1. Strain Selection and Serotyping ... All strains were propagated on Luria Bertani (LB) agar or broth (Becton Dickinson [BD], Mississauga, ON) and archived at −80°C in LB broth with 20% glycerol (Sigma Aldrich, Oakville, ON).

Concentration Assay:

Article Title: Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain
Article Snippet: The final concentration 5 × 105 c.f.u. ml−1 of bacteria or fungi was maintained in each well of microtitre plate. .. Briefly, the microbial inoculum (5 × 105 c.f.u. ml−1 ) was diluted with appropriate broth (1:1000) and 100 µl was then spread on the surface of Tripticase Soy Agar (TSA, Becton Dickinson) or Sabouraud Dextrose Agar (SDA, Becton Dickinson).

Staining:

Article Title: Capsular Polysaccharide Surrounds Smooth and Rugose Types of Salmonella enterica serovar Typhimurium DT104 † serovar Typhimurium DT104 † ‡
Article Snippet: Serovar Typhimurium DT104 and E. coli were cultured on Trypticase soy agar and Luria-Bertani (LB) agar and broth (Becton Dickinson, Franklin Lakes, NJ) at 25°C and 37°C for the formation of rugose (Rv/25)- and smooth (Rv/37)-colony variant phenotypes, respectively ( ). .. Capsules were observed in negatively stained India ink preparations following the standard Duguid protocol ( ).

Variant Assay:

Article Title: Capsular Polysaccharide Surrounds Smooth and Rugose Types of Salmonella enterica serovar Typhimurium DT104 † serovar Typhimurium DT104 † ‡
Article Snippet: .. Serovar Typhimurium DT104 and E. coli were cultured on Trypticase soy agar and Luria-Bertani (LB) agar and broth (Becton Dickinson, Franklin Lakes, NJ) at 25°C and 37°C for the formation of rugose (Rv/25)- and smooth (Rv/37)-colony variant phenotypes, respectively ( ). .. A stable, smooth, spontaneous mutant of serovar Typhimurium DT104 (Stv) , which forms smooth colonies at both temperatures, was used as a negative control in all experiments.

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    Becton Dickinson tryptic soy broth dextrose free tsb medium
    Immunoblots of GFP in R. <t>eutropha</t> Δ phaR phaP-gfp , Δ phaR Δ phaC phaP-gfp , and control strains. Proteins were separated by SDS-PAGE and then subjected to immunoblot analysis for detection of GFP. Molecular mass standards are indicated in kilodaltons. Cells from R. eutropha cultures were harvested after cultivation for 48 h in <t>TSB</t> or PHA(high) and resuspended in 10 μl at an OD 600 of 0.4 for analysis. The E. coli strains DH5α/pGY1a+, which carries the phaP-gfp fusion on a plasmid, and DH5α/pSW213, which carries the corresponding vector lacking the phaP-gfp fusion, were also included as positive and negative controls, respectively (24-h cultivation in LB plus tetracycline, resuspended in 10 μl at an OD 600 of 1.0 for analysis). Culture OD 600 measurements were as follows: lane 1, 9.0; lane 2, 2.7; lane 3, 9.3; lane 4, 1.5; lane 5, 9.2; lane 6, 4.8; lane 7, 9.7; lane 8, 1.5; lane 9, 8.8; lane 10, 14; lane 11, 9.2; lane 12, 5.0; lane 13, 4.2; lane 14, 4.0.
    Tryptic Soy Broth Dextrose Free Tsb Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tryptic soy broth dextrose free tsb medium/product/Becton Dickinson
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tryptic soy broth dextrose free tsb medium - by Bioz Stars, 2020-04
    85/100 stars
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    95
    Becton Dickinson mueller hinton broth
    Effect of anthocyanins on sec A transcription. H. pylori was cultured with 100 μM of anthocyanin in <t>Mueller-Hinton</t> broth/10% FBS for 3 days. After extensive washing, total RNA was extracted and cDNA synthesized. RT-PCR analysis was performed to assess expression of sec A, cag A, and vac A. The galE (UDP-galactose 4-epimerase gene) was used as an internal control. No RT, no reverse transcriptase. A representative image from five independent experiments.
    Mueller Hinton Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mueller hinton broth/product/Becton Dickinson
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mueller hinton broth - by Bioz Stars, 2020-04
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    95
    Becton Dickinson 7h9 broth
    Mtb MazG is required for resistance to RNS shown in vitro and ex vivo . ( A ) mazG -null Mtb is susceptible to killing by acid nitrite. Exponential phase cultures (OD 600 ∼0.5) were suspended in <t>7H9-OADC</t> pH4.5 with or without 2.5 mM NaNO 2 and treated for 20 hours. wt, wild-type Mtb ; Δ mazG , mazG -null Mtb ; compl, the complemented mutant. Data shown are mean ± S.E. of triplicates. * p
    7h9 Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 broth/product/Becton Dickinson
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    7h9 broth - by Bioz Stars, 2020-04
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    85
    Becton Dickinson ypd broth previous
    Effects of SNF7 disruption on fungal growth under different conditions. (A). Wild type (WT), mutant ( snf7Δ ) and complemented ( snf7Δ::SNF7 ) strains of C. <t>neoformans</t> and C. gattii were grown in <t>YPD</t> broth with shaking at 30°C or 37°C. Growth measurements (OD 600 ) were performed at 4 h, 6 h, 8 h, 10 h, 12 h and 24 h. (B). Ten-fold serial dilutions of all strains were plated on LCM + Cu and LCM agar plates. The plates were incubated for 2 days at 30°C or 37°C. Fungal growth in LCM + Cu represented the control condition. (C). Growth rates of WT, mutant and complemented strains at pHs 7, 7.5, 8, 8.5 and 9. (D). Growth of WT, snf7Δ and snf7Δ::SNF7 strains on YPD agar plates supplemented with 150 mM LiCl 2 . Experiments were performed in triplicate and representative examples are shown.
    Ypd Broth Previous, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ypd broth previous/product/Becton Dickinson
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    Immunoblots of GFP in R. eutropha Δ phaR phaP-gfp , Δ phaR Δ phaC phaP-gfp , and control strains. Proteins were separated by SDS-PAGE and then subjected to immunoblot analysis for detection of GFP. Molecular mass standards are indicated in kilodaltons. Cells from R. eutropha cultures were harvested after cultivation for 48 h in TSB or PHA(high) and resuspended in 10 μl at an OD 600 of 0.4 for analysis. The E. coli strains DH5α/pGY1a+, which carries the phaP-gfp fusion on a plasmid, and DH5α/pSW213, which carries the corresponding vector lacking the phaP-gfp fusion, were also included as positive and negative controls, respectively (24-h cultivation in LB plus tetracycline, resuspended in 10 μl at an OD 600 of 1.0 for analysis). Culture OD 600 measurements were as follows: lane 1, 9.0; lane 2, 2.7; lane 3, 9.3; lane 4, 1.5; lane 5, 9.2; lane 6, 4.8; lane 7, 9.7; lane 8, 1.5; lane 9, 8.8; lane 10, 14; lane 11, 9.2; lane 12, 5.0; lane 13, 4.2; lane 14, 4.0.

    Journal: Journal of Bacteriology

    Article Title: The Ralstonia eutropha PhaR Protein Couples Synthesis of the PhaP Phasin to the Presence of Polyhydroxybutyrate in Cells and Promotes Polyhydroxybutyrate Production

    doi: 10.1128/JB.184.1.59-66.2002

    Figure Lengend Snippet: Immunoblots of GFP in R. eutropha Δ phaR phaP-gfp , Δ phaR Δ phaC phaP-gfp , and control strains. Proteins were separated by SDS-PAGE and then subjected to immunoblot analysis for detection of GFP. Molecular mass standards are indicated in kilodaltons. Cells from R. eutropha cultures were harvested after cultivation for 48 h in TSB or PHA(high) and resuspended in 10 μl at an OD 600 of 0.4 for analysis. The E. coli strains DH5α/pGY1a+, which carries the phaP-gfp fusion on a plasmid, and DH5α/pSW213, which carries the corresponding vector lacking the phaP-gfp fusion, were also included as positive and negative controls, respectively (24-h cultivation in LB plus tetracycline, resuspended in 10 μl at an OD 600 of 1.0 for analysis). Culture OD 600 measurements were as follows: lane 1, 9.0; lane 2, 2.7; lane 3, 9.3; lane 4, 1.5; lane 5, 9.2; lane 6, 4.8; lane 7, 9.7; lane 8, 1.5; lane 9, 8.8; lane 10, 14; lane 11, 9.2; lane 12, 5.0; lane 13, 4.2; lane 14, 4.0.

    Article Snippet: R. eutropha strains were cultivated in Luria-Bertani (LB) medium , Tryptic Soy Broth-Dextrose Free (TSB) medium (Becton Dickinson Microbiology Systems, Cockeysville, Md.), PHA(med), and PHA(high).

    Techniques: Western Blot, SDS Page, Plasmid Preparation

    Immunoblots of PhaP in R. eutropha strains. Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were subjected to immunoblot analysis for detection of PhaP. Cells from R. eutropha cultures were harvested after cultivation for 48 h in TSB, PHA(med), or PHA(high). Bacterial samples correspond to cells from 10 μl of a culture diluted to an OD 600 of 0.2. Purified PhaP was included as a control. Molecular mass standards are indicated in kilodaltons. Culture OD 600 measurements were as follows: lane 1, 8.9; lane 2, 8.3; lane 3, 9.6; lane 4, 8.6; lane 5, 7.2; lane 6, 5.7; lane 7, 9.1; lane 8, 4.3; lane 9, 1.3; lane 10, 8.9; lane11, 3.8; lane 12, 1.8.

    Journal: Journal of Bacteriology

    Article Title: The Ralstonia eutropha PhaR Protein Couples Synthesis of the PhaP Phasin to the Presence of Polyhydroxybutyrate in Cells and Promotes Polyhydroxybutyrate Production

    doi: 10.1128/JB.184.1.59-66.2002

    Figure Lengend Snippet: Immunoblots of PhaP in R. eutropha strains. Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were subjected to immunoblot analysis for detection of PhaP. Cells from R. eutropha cultures were harvested after cultivation for 48 h in TSB, PHA(med), or PHA(high). Bacterial samples correspond to cells from 10 μl of a culture diluted to an OD 600 of 0.2. Purified PhaP was included as a control. Molecular mass standards are indicated in kilodaltons. Culture OD 600 measurements were as follows: lane 1, 8.9; lane 2, 8.3; lane 3, 9.6; lane 4, 8.6; lane 5, 7.2; lane 6, 5.7; lane 7, 9.1; lane 8, 4.3; lane 9, 1.3; lane 10, 8.9; lane11, 3.8; lane 12, 1.8.

    Article Snippet: R. eutropha strains were cultivated in Luria-Bertani (LB) medium , Tryptic Soy Broth-Dextrose Free (TSB) medium (Becton Dickinson Microbiology Systems, Cockeysville, Md.), PHA(med), and PHA(high).

    Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, SDS Page, Purification

    Effect of anthocyanins on sec A transcription. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days. After extensive washing, total RNA was extracted and cDNA synthesized. RT-PCR analysis was performed to assess expression of sec A, cag A, and vac A. The galE (UDP-galactose 4-epimerase gene) was used as an internal control. No RT, no reverse transcriptase. A representative image from five independent experiments.

    Journal: International Journal of Medical Sciences

    Article Title: Inhibitory Effects of Anthocyanins on Secretion of Helicobacter pylori CagA and VacA Toxins

    doi: 10.7150/ijms.5094

    Figure Lengend Snippet: Effect of anthocyanins on sec A transcription. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days. After extensive washing, total RNA was extracted and cDNA synthesized. RT-PCR analysis was performed to assess expression of sec A, cag A, and vac A. The galE (UDP-galactose 4-epimerase gene) was used as an internal control. No RT, no reverse transcriptase. A representative image from five independent experiments.

    Article Snippet: The various anthocyanins (C3G, Peo3G, Pel3G, and M3G) were added to the Mueller-Hinton broth at a final concentration of 100 μM.

    Techniques: Size-exclusion Chromatography, Cell Culture, Synthesized, Reverse Transcription Polymerase Chain Reaction, Expressing

    Effect of anthocyanins on secretion of H. pylori CagA and VacA. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days and proteins assessed by Western blot. (A), secreted CagA and VacA in culture media. (B), secreted H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. (C) , intracellular CagA and VacA. (D) , intracellular H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. Representative image from five independent experiments.

    Journal: International Journal of Medical Sciences

    Article Title: Inhibitory Effects of Anthocyanins on Secretion of Helicobacter pylori CagA and VacA Toxins

    doi: 10.7150/ijms.5094

    Figure Lengend Snippet: Effect of anthocyanins on secretion of H. pylori CagA and VacA. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days and proteins assessed by Western blot. (A), secreted CagA and VacA in culture media. (B), secreted H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. (C) , intracellular CagA and VacA. (D) , intracellular H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. Representative image from five independent experiments.

    Article Snippet: The various anthocyanins (C3G, Peo3G, Pel3G, and M3G) were added to the Mueller-Hinton broth at a final concentration of 100 μM.

    Techniques: Cell Culture, Western Blot

    Mtb MazG is required for resistance to RNS shown in vitro and ex vivo . ( A ) mazG -null Mtb is susceptible to killing by acid nitrite. Exponential phase cultures (OD 600 ∼0.5) were suspended in 7H9-OADC pH4.5 with or without 2.5 mM NaNO 2 and treated for 20 hours. wt, wild-type Mtb ; Δ mazG , mazG -null Mtb ; compl, the complemented mutant. Data shown are mean ± S.E. of triplicates. * p

    Journal: PLoS Pathogens

    Article Title: Mycobacterial MazG Safeguards Genetic Stability via Housecleaning of 5-OH-dCTP

    doi: 10.1371/journal.ppat.1003814

    Figure Lengend Snippet: Mtb MazG is required for resistance to RNS shown in vitro and ex vivo . ( A ) mazG -null Mtb is susceptible to killing by acid nitrite. Exponential phase cultures (OD 600 ∼0.5) were suspended in 7H9-OADC pH4.5 with or without 2.5 mM NaNO 2 and treated for 20 hours. wt, wild-type Mtb ; Δ mazG , mazG -null Mtb ; compl, the complemented mutant. Data shown are mean ± S.E. of triplicates. * p

    Article Snippet: Msm strains were grown at 37°C in 7H9 broth (BD Difco), or on Luria-Bertani agar supplemented with 0.5% glycerol (LBG agar).

    Techniques: In Vitro, Ex Vivo, Mutagenesis

    Effects of SNF7 disruption on fungal growth under different conditions. (A). Wild type (WT), mutant ( snf7Δ ) and complemented ( snf7Δ::SNF7 ) strains of C. neoformans and C. gattii were grown in YPD broth with shaking at 30°C or 37°C. Growth measurements (OD 600 ) were performed at 4 h, 6 h, 8 h, 10 h, 12 h and 24 h. (B). Ten-fold serial dilutions of all strains were plated on LCM + Cu and LCM agar plates. The plates were incubated for 2 days at 30°C or 37°C. Fungal growth in LCM + Cu represented the control condition. (C). Growth rates of WT, mutant and complemented strains at pHs 7, 7.5, 8, 8.5 and 9. (D). Growth of WT, snf7Δ and snf7Δ::SNF7 strains on YPD agar plates supplemented with 150 mM LiCl 2 . Experiments were performed in triplicate and representative examples are shown.

    Journal: Scientific Reports

    Article Title: The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex.

    doi: 10.1038/srep06198

    Figure Lengend Snippet: Effects of SNF7 disruption on fungal growth under different conditions. (A). Wild type (WT), mutant ( snf7Δ ) and complemented ( snf7Δ::SNF7 ) strains of C. neoformans and C. gattii were grown in YPD broth with shaking at 30°C or 37°C. Growth measurements (OD 600 ) were performed at 4 h, 6 h, 8 h, 10 h, 12 h and 24 h. (B). Ten-fold serial dilutions of all strains were plated on LCM + Cu and LCM agar plates. The plates were incubated for 2 days at 30°C or 37°C. Fungal growth in LCM + Cu represented the control condition. (C). Growth rates of WT, mutant and complemented strains at pHs 7, 7.5, 8, 8.5 and 9. (D). Growth of WT, snf7Δ and snf7Δ::SNF7 strains on YPD agar plates supplemented with 150 mM LiCl 2 . Experiments were performed in triplicate and representative examples are shown.

    Article Snippet: To evaluate the effects of Cu2+ deprivation on C. neoformans and C. gattii , cells were grown in 10 mL of YPD broth previous to a further cultivation in 100 mL of Yeast Nitrogen Base broth (YNB) (Becton, Dickinson and Company, Sparks, USA) for 24 hours at 37°C.

    Techniques: Mutagenesis, Laser Capture Microdissection, Incubation