brg1 antibody  (Abcam)

 
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    Name:
    Anti BRG1 antibody
    Description:

    Catalog Number:
    ab4081
    Price:
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    Structured Review

    Abcam brg1 antibody
    <t>BRG1</t> plays a tumor suppressor role. (A) Colony formation of 5637-NC and 5637-iBRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (B) Colony formation of 5637-pcDNA3.1 and 5637-BRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (C) Growth curves of 5637 cells after transfection with BRG1 RNAi. Cellular proliferation was measured using MTT assay at 24, 48, 72, 96 and 120 h. The differences in data of 72, 96 and 120 h were significant, * P

    https://www.bioz.com/result/brg1 antibody/product/Abcam
    Average 90 stars, based on 3 article reviews
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    brg1 antibody - by Bioz Stars, 2020-01
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    Images

    1) Product Images from "Long non-coding RNA urothelial carcinoma associated 1 induces cell replication by inhibiting BRG1 in 5637 cells"

    Article Title: Long non-coding RNA urothelial carcinoma associated 1 induces cell replication by inhibiting BRG1 in 5637 cells

    Journal: Oncology Reports

    doi: 10.3892/or.2014.3309

    BRG1 plays a tumor suppressor role. (A) Colony formation of 5637-NC and 5637-iBRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (B) Colony formation of 5637-pcDNA3.1 and 5637-BRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (C) Growth curves of 5637 cells after transfection with BRG1 RNAi. Cellular proliferation was measured using MTT assay at 24, 48, 72, 96 and 120 h. The differences in data of 72, 96 and 120 h were significant, * P
    Figure Legend Snippet: BRG1 plays a tumor suppressor role. (A) Colony formation of 5637-NC and 5637-iBRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (B) Colony formation of 5637-pcDNA3.1 and 5637-BRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (C) Growth curves of 5637 cells after transfection with BRG1 RNAi. Cellular proliferation was measured using MTT assay at 24, 48, 72, 96 and 120 h. The differences in data of 72, 96 and 120 h were significant, * P

    Techniques Used: Cell Culture, Staining, Transfection, MTT Assay

    UCA1 blocks recruitment of BRG1 to chromatin. (A) UCA1 does not affect the ATPase activity of BRG1. The kinetics of BRG1-induced ATP hydrolysis were analyzed in the presence or absence of UCA1. (B) ChIP analysis of BRG1 binding to the p21 promoter in 5637-iUCA1. 5637-NC cells were used as the control. Genomic DNA was fixed and immunoprecipitated using anti-BRG1 antibody, with IgG as a negative control. Real-time PCR was performed using a primer set specific to the BRG1-binding site of p21 promoter. Data were normalized to input and are expressed as the means ± SD of three independent experiments. * P
    Figure Legend Snippet: UCA1 blocks recruitment of BRG1 to chromatin. (A) UCA1 does not affect the ATPase activity of BRG1. The kinetics of BRG1-induced ATP hydrolysis were analyzed in the presence or absence of UCA1. (B) ChIP analysis of BRG1 binding to the p21 promoter in 5637-iUCA1. 5637-NC cells were used as the control. Genomic DNA was fixed and immunoprecipitated using anti-BRG1 antibody, with IgG as a negative control. Real-time PCR was performed using a primer set specific to the BRG1-binding site of p21 promoter. Data were normalized to input and are expressed as the means ± SD of three independent experiments. * P

    Techniques Used: Activity Assay, Chromatin Immunoprecipitation, Binding Assay, Immunoprecipitation, Negative Control, Real-time Polymerase Chain Reaction

    UCA1 binds to BRG1 in vitro and in vivo . (A) Electrophoresis of proteins bound to biotin-labeled sense and antisense UCA1 in NuPAGE 4–12% Bis-Tris gel. The biotin-labeled sense and antisense UCA1 were transcribed in vitro and incubated with HeLa cell lysate. The arrow shows the protein band for BRG1. (B) Western blot analysis of biotin-labeled sense and antisense UCA1-bound proteins using antibody against BRG1. (C) UCA1 binding BRG1 in 5637 cells was detected by RNA-binding protein immunoprecipitation assay. Antibody against BRG1 and mouse IgG (as a negative control) were used to pull-down RNAs in 5637 cells. The level of UCA1 was determined using real-time PCR. Data were normalized to input and are expressed as the means ± SD of three independent experiments * P
    Figure Legend Snippet: UCA1 binds to BRG1 in vitro and in vivo . (A) Electrophoresis of proteins bound to biotin-labeled sense and antisense UCA1 in NuPAGE 4–12% Bis-Tris gel. The biotin-labeled sense and antisense UCA1 were transcribed in vitro and incubated with HeLa cell lysate. The arrow shows the protein band for BRG1. (B) Western blot analysis of biotin-labeled sense and antisense UCA1-bound proteins using antibody against BRG1. (C) UCA1 binding BRG1 in 5637 cells was detected by RNA-binding protein immunoprecipitation assay. Antibody against BRG1 and mouse IgG (as a negative control) were used to pull-down RNAs in 5637 cells. The level of UCA1 was determined using real-time PCR. Data were normalized to input and are expressed as the means ± SD of three independent experiments * P

    Techniques Used: In Vitro, In Vivo, Electrophoresis, Labeling, Incubation, Western Blot, Binding Assay, RNA Binding Assay, Immunoprecipitation, Negative Control, Real-time Polymerase Chain Reaction

    UCA1 antagonizes the tumor suppressing function of BRG1. (A) (Left) Colony formation of EJ control, EJ-BRG1 (overexpressed BRG1) and EJ-BRG1-UCA1 (overexpressed BRG1 and UCA1) cells. The cells were cultured with G418 for 10 days. Colonies were stained using crystal violet. (Right) Colony formation of 5637 control, 5637-iUCA1 (UCA1 knocked down) and 5637-iUCA1-iBRG1 (UCA1 and BRG1 both knocked down) cells. The cells were cultured with G418 and puromycin for 10 days. Colonies were stained using crystal violet. (B) (Left) Growth curves of EJ control, EJ-BRG1 and EJ-BRG1-UCA1 cells. Cellular proliferation was measured using MTT assays at 24, 48, 72, 96 and 120 h. The differences between EJ-BRG1 and EJ-BRG1-UCA1 were significant. * P
    Figure Legend Snippet: UCA1 antagonizes the tumor suppressing function of BRG1. (A) (Left) Colony formation of EJ control, EJ-BRG1 (overexpressed BRG1) and EJ-BRG1-UCA1 (overexpressed BRG1 and UCA1) cells. The cells were cultured with G418 for 10 days. Colonies were stained using crystal violet. (Right) Colony formation of 5637 control, 5637-iUCA1 (UCA1 knocked down) and 5637-iUCA1-iBRG1 (UCA1 and BRG1 both knocked down) cells. The cells were cultured with G418 and puromycin for 10 days. Colonies were stained using crystal violet. (B) (Left) Growth curves of EJ control, EJ-BRG1 and EJ-BRG1-UCA1 cells. Cellular proliferation was measured using MTT assays at 24, 48, 72, 96 and 120 h. The differences between EJ-BRG1 and EJ-BRG1-UCA1 were significant. * P

    Techniques Used: Cell Culture, Staining, MTT Assay

    UCA1 expression correlates positively with BRG1 expression in bladder cancer tissue samples. (A) Real-time PCR was used to analyze UCA1 and BRG1 mRNA levels in the same samples. S, sample. (B) The status of UCA1 and BRG1 expression in bladder cancer specimens, > 2-fold was identified as high expression. The 2×2 correlation table and Fisher’s exact test were used. The two-sided value P=0.009 was considered significant. UCA1, urothelial carcinoma associated 1.
    Figure Legend Snippet: UCA1 expression correlates positively with BRG1 expression in bladder cancer tissue samples. (A) Real-time PCR was used to analyze UCA1 and BRG1 mRNA levels in the same samples. S, sample. (B) The status of UCA1 and BRG1 expression in bladder cancer specimens, > 2-fold was identified as high expression. The 2×2 correlation table and Fisher’s exact test were used. The two-sided value P=0.009 was considered significant. UCA1, urothelial carcinoma associated 1.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    2) Product Images from "The dilemma of early preventive oophorectomy in familial small cell carcinoma of the ovary of hypercalcemic type"

    Article Title: The dilemma of early preventive oophorectomy in familial small cell carcinoma of the ovary of hypercalcemic type

    Journal: Gynecologic Oncology Reports

    doi: 10.1016/j.gore.2019.02.002

    Retained SMARCA4 (BRG1) protein expression in one of the ovaries. There is positive nuclear staining of the granulosa cells of a developing follicle (right of photomicrograph) and the ovarian stroma (left of photomicrograph).
    Figure Legend Snippet: Retained SMARCA4 (BRG1) protein expression in one of the ovaries. There is positive nuclear staining of the granulosa cells of a developing follicle (right of photomicrograph) and the ovarian stroma (left of photomicrograph).

    Techniques Used: Expressing, Staining

    3) Product Images from "The spring-loaded genome: Nucleosome redistributions are widespread, transient, and DNA-directed"

    Article Title: The spring-loaded genome: Nucleosome redistributions are widespread, transient, and DNA-directed

    Journal: Genome Research

    doi: 10.1101/gr.160150.113

    Nucleosome redistributions are determined by the underlying DNA sequence. ( A ) Western blots with the specified antibodies, at various times (in hours) after KSHV reactivation (hpr), of iSLK.219 cells treated with 0.2 µg/mL doxycycline. BRG1 protein
    Figure Legend Snippet: Nucleosome redistributions are determined by the underlying DNA sequence. ( A ) Western blots with the specified antibodies, at various times (in hours) after KSHV reactivation (hpr), of iSLK.219 cells treated with 0.2 µg/mL doxycycline. BRG1 protein

    Techniques Used: Sequencing, Western Blot

    4) Product Images from "DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection"

    Article Title: DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection

    Journal: Oncogene

    doi: 10.1038/onc.2016.399

    BRG1/BAF complex is involved in SALL4 re-expression in HBV replicating hepatocytes
    Figure Legend Snippet: BRG1/BAF complex is involved in SALL4 re-expression in HBV replicating hepatocytes

    Techniques Used: Expressing

    HBV replication enables binding and interaction of STAT3, OCT4 and BRG1 in SALL4 regulatory region
    Figure Legend Snippet: HBV replication enables binding and interaction of STAT3, OCT4 and BRG1 in SALL4 regulatory region

    Techniques Used: Binding Assay

    5) Product Images from "DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection"

    Article Title: DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection

    Journal: Oncogene

    doi: 10.1038/onc.2016.399

    BRG1/BAF complex is involved in SALL4 re-expression in HBV replicating hepatocytes ( a, b ) PCR quantification of SALL4 mRNA (Left panel), and immunoblots of SALL4 (Right panel), following transfection of BRG1 siRNA (siBRG1) ( a ), and BRG1 plasmid ( b ), in HepAD38 cells grown with HBV replication by tetracycline removal for 10 days. Results are from three independent RNA isolations performed in identical triplicates. Error bars represent S.D. * P
    Figure Legend Snippet: BRG1/BAF complex is involved in SALL4 re-expression in HBV replicating hepatocytes ( a, b ) PCR quantification of SALL4 mRNA (Left panel), and immunoblots of SALL4 (Right panel), following transfection of BRG1 siRNA (siBRG1) ( a ), and BRG1 plasmid ( b ), in HepAD38 cells grown with HBV replication by tetracycline removal for 10 days. Results are from three independent RNA isolations performed in identical triplicates. Error bars represent S.D. * P

    Techniques Used: Expressing, Polymerase Chain Reaction, Western Blot, Transfection, Plasmid Preparation

    HBV replication enables binding and interaction of STAT3, OCT4 and BRG1 in SALL4 regulatory region ( a ) ChIP assays with STAT3, OCT4, and BRG1 antibodies, as indicated, employing HepAD38 cells grown −/+ HBV replication by tetracycline removal for D0 and D10, and SALL4 primers spanning CpG sites shown in Fig.1b . Data were quantified as % of input. ( b ) Co-immunoprecipitations (co-IPs) of STAT3, OCT4 and BRG1 using indicated antibodies, employing extracts from HepAD38 cells grown −/+ HBV replication by tetracycline removal for D0 and D10. A representative assay is shown from three independent experiments. ( c ) Sequential ChIP assays of HepAD38 cells −/+ HBV replication by tetracycline removal for D0 and D10, using BRG1 antibody followed by tandem IP with STAT3 or OCT4 antibody. Results are from three independent experiments. Data were quantified as % of input.
    Figure Legend Snippet: HBV replication enables binding and interaction of STAT3, OCT4 and BRG1 in SALL4 regulatory region ( a ) ChIP assays with STAT3, OCT4, and BRG1 antibodies, as indicated, employing HepAD38 cells grown −/+ HBV replication by tetracycline removal for D0 and D10, and SALL4 primers spanning CpG sites shown in Fig.1b . Data were quantified as % of input. ( b ) Co-immunoprecipitations (co-IPs) of STAT3, OCT4 and BRG1 using indicated antibodies, employing extracts from HepAD38 cells grown −/+ HBV replication by tetracycline removal for D0 and D10. A representative assay is shown from three independent experiments. ( c ) Sequential ChIP assays of HepAD38 cells −/+ HBV replication by tetracycline removal for D0 and D10, using BRG1 antibody followed by tandem IP with STAT3 or OCT4 antibody. Results are from three independent experiments. Data were quantified as % of input.

    Techniques Used: Binding Assay, Chromatin Immunoprecipitation

    6) Product Images from "Long non-coding RNA urothelial carcinoma associated 1 induces cell replication by inhibiting BRG1 in 5637 cells"

    Article Title: Long non-coding RNA urothelial carcinoma associated 1 induces cell replication by inhibiting BRG1 in 5637 cells

    Journal: Oncology Reports

    doi: 10.3892/or.2014.3309

    BRG1 plays a tumor suppressor role. (A) Colony formation of 5637-NC and 5637-iBRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (B) Colony formation of 5637-pcDNA3.1 and 5637-BRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (C) Growth curves of 5637 cells after transfection with BRG1 RNAi. Cellular proliferation was measured using MTT assay at 24, 48, 72, 96 and 120 h. The differences in data of 72, 96 and 120 h were significant, * P
    Figure Legend Snippet: BRG1 plays a tumor suppressor role. (A) Colony formation of 5637-NC and 5637-iBRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (B) Colony formation of 5637-pcDNA3.1 and 5637-BRG1 cells. The cells were cultured for 14 days. Colonies were stained using crystal violet. (C) Growth curves of 5637 cells after transfection with BRG1 RNAi. Cellular proliferation was measured using MTT assay at 24, 48, 72, 96 and 120 h. The differences in data of 72, 96 and 120 h were significant, * P

    Techniques Used: Cell Culture, Staining, Transfection, MTT Assay

    UCA1 blocks recruitment of BRG1 to chromatin. (A) UCA1 does not affect the ATPase activity of BRG1. The kinetics of BRG1-induced ATP hydrolysis were analyzed in the presence or absence of UCA1. (B) ChIP analysis of BRG1 binding to the p21 promoter in 5637-iUCA1. 5637-NC cells were used as the control. Genomic DNA was fixed and immunoprecipitated using anti-BRG1 antibody, with IgG as a negative control. Real-time PCR was performed using a primer set specific to the BRG1-binding site of p21 promoter. Data were normalized to input and are expressed as the means ± SD of three independent experiments. * P
    Figure Legend Snippet: UCA1 blocks recruitment of BRG1 to chromatin. (A) UCA1 does not affect the ATPase activity of BRG1. The kinetics of BRG1-induced ATP hydrolysis were analyzed in the presence or absence of UCA1. (B) ChIP analysis of BRG1 binding to the p21 promoter in 5637-iUCA1. 5637-NC cells were used as the control. Genomic DNA was fixed and immunoprecipitated using anti-BRG1 antibody, with IgG as a negative control. Real-time PCR was performed using a primer set specific to the BRG1-binding site of p21 promoter. Data were normalized to input and are expressed as the means ± SD of three independent experiments. * P

    Techniques Used: Activity Assay, Chromatin Immunoprecipitation, Binding Assay, Immunoprecipitation, Negative Control, Real-time Polymerase Chain Reaction

    UCA1 binds to BRG1 in vitro and in vivo . (A) Electrophoresis of proteins bound to biotin-labeled sense and antisense UCA1 in NuPAGE 4–12% Bis-Tris gel. The biotin-labeled sense and antisense UCA1 were transcribed in vitro and incubated with HeLa cell lysate. The arrow shows the protein band for BRG1. (B) Western blot analysis of biotin-labeled sense and antisense UCA1-bound proteins using antibody against BRG1. (C) UCA1 binding BRG1 in 5637 cells was detected by RNA-binding protein immunoprecipitation assay. Antibody against BRG1 and mouse IgG (as a negative control) were used to pull-down RNAs in 5637 cells. The level of UCA1 was determined using real-time PCR. Data were normalized to input and are expressed as the means ± SD of three independent experiments * P
    Figure Legend Snippet: UCA1 binds to BRG1 in vitro and in vivo . (A) Electrophoresis of proteins bound to biotin-labeled sense and antisense UCA1 in NuPAGE 4–12% Bis-Tris gel. The biotin-labeled sense and antisense UCA1 were transcribed in vitro and incubated with HeLa cell lysate. The arrow shows the protein band for BRG1. (B) Western blot analysis of biotin-labeled sense and antisense UCA1-bound proteins using antibody against BRG1. (C) UCA1 binding BRG1 in 5637 cells was detected by RNA-binding protein immunoprecipitation assay. Antibody against BRG1 and mouse IgG (as a negative control) were used to pull-down RNAs in 5637 cells. The level of UCA1 was determined using real-time PCR. Data were normalized to input and are expressed as the means ± SD of three independent experiments * P

    Techniques Used: In Vitro, In Vivo, Electrophoresis, Labeling, Incubation, Western Blot, Binding Assay, RNA Binding Assay, Immunoprecipitation, Negative Control, Real-time Polymerase Chain Reaction

    UCA1 antagonizes the tumor suppressing function of BRG1. (A) (Left) Colony formation of EJ control, EJ-BRG1 (overexpressed BRG1) and EJ-BRG1-UCA1 (overexpressed BRG1 and UCA1) cells. The cells were cultured with G418 for 10 days. Colonies were stained using crystal violet. (Right) Colony formation of 5637 control, 5637-iUCA1 (UCA1 knocked down) and 5637-iUCA1-iBRG1 (UCA1 and BRG1 both knocked down) cells. The cells were cultured with G418 and puromycin for 10 days. Colonies were stained using crystal violet. (B) (Left) Growth curves of EJ control, EJ-BRG1 and EJ-BRG1-UCA1 cells. Cellular proliferation was measured using MTT assays at 24, 48, 72, 96 and 120 h. The differences between EJ-BRG1 and EJ-BRG1-UCA1 were significant. * P
    Figure Legend Snippet: UCA1 antagonizes the tumor suppressing function of BRG1. (A) (Left) Colony formation of EJ control, EJ-BRG1 (overexpressed BRG1) and EJ-BRG1-UCA1 (overexpressed BRG1 and UCA1) cells. The cells were cultured with G418 for 10 days. Colonies were stained using crystal violet. (Right) Colony formation of 5637 control, 5637-iUCA1 (UCA1 knocked down) and 5637-iUCA1-iBRG1 (UCA1 and BRG1 both knocked down) cells. The cells were cultured with G418 and puromycin for 10 days. Colonies were stained using crystal violet. (B) (Left) Growth curves of EJ control, EJ-BRG1 and EJ-BRG1-UCA1 cells. Cellular proliferation was measured using MTT assays at 24, 48, 72, 96 and 120 h. The differences between EJ-BRG1 and EJ-BRG1-UCA1 were significant. * P

    Techniques Used: Cell Culture, Staining, MTT Assay

    UCA1 expression correlates positively with BRG1 expression in bladder cancer tissue samples. (A) Real-time PCR was used to analyze UCA1 and BRG1 mRNA levels in the same samples. S, sample. (B) The status of UCA1 and BRG1 expression in bladder cancer specimens, > 2-fold was identified as high expression. The 2×2 correlation table and Fisher’s exact test were used. The two-sided value P=0.009 was considered significant. UCA1, urothelial carcinoma associated 1.
    Figure Legend Snippet: UCA1 expression correlates positively with BRG1 expression in bladder cancer tissue samples. (A) Real-time PCR was used to analyze UCA1 and BRG1 mRNA levels in the same samples. S, sample. (B) The status of UCA1 and BRG1 expression in bladder cancer specimens, > 2-fold was identified as high expression. The 2×2 correlation table and Fisher’s exact test were used. The two-sided value P=0.009 was considered significant. UCA1, urothelial carcinoma associated 1.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    Related Articles

    Transduction:

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: Cross-linked chromatin was prepared from ∼2 × 107 cells 48 h after transduction with lentiviruses expressing either hSNF5 or GFP as a control. .. The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]).

    Clone Assay:

    Article Title: BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens
    Article Snippet: Immunoprecipitation and western blotting WT Baf53b and Baf53bΔHD were cloned into pLVX-EF1a-IRES-mCherry (Clontech, Mountain View, CA) creating plasmids MW95 and MW97, respectively. .. Immunoprecipitations against Brg1 were performed using 1 μg of antibody ab70558 (Abcam,, Cambridge, MA).

    Centrifugation:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C. .. Beads were pelleted by centrifugation and washed three times with 1 mL of wash buffer.

    Amplification:

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA. .. To prepare libraries for sequencing, DNA was end repaired, A-tailed, adapter ligated (Illumina TrueSeq) and PCR amplified.

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]). .. The abundance of specific DNA sequences in the immunoprecipitates was determined by qPCR and corrected for the independently determined amplification curves of each primer set.

    Construct:

    Article Title: Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2–β-Catenin–BRG1 Transcriptional Network-Driven CD47 Expression
    Article Snippet: .. Western blot analysis was performed with protein from whole-cell extracts, cytosolic extracts, and nuclear extracts of cells transfected with different constructs or siRNA and treated with different inhibitors and activators as described previously , using mouse monoclonal anti-CD47 (1:1,000) (catalog no. ab9089; Abcam, Cambridge, UK), rabbit monoclonal anti-PKM2 (1:1,000) (catalog no. 4053; Cell Signaling, Boston, MA), rabbit monoclonal anti-GSK-3β (1:1,000) (catalog no. 9315; Cell Signaling), rabbit polyclonal anti-non-phospho-β-catenin (1:1,000) (catalog no. 4270; Cell Signaling), rabbit monoclonal anti-β-catenin (1:1,000) (catalog no. 8480; Cell Signaling), mouse monoclonal anti-β-catenin (1:1,000) (catalog no. ab19450; Abcam), goat polyclonal anti-TCF4 (1:1,000) (catalog no. sc-8631; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-BRG1 (1:1,000) (catalog no. ab4081; Abcam), mouse monoclonal anti-IDH1-R132H (clone HMab-1; Sigma-Aldrich), and rabbit polyclonal anti-IDH1 (1:1,000) (catalog no. ab135659; Abcam). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Vector Laboratories Inc. (Burlingame, CA).

    Article Title: BRG1 promotes the repair of DNA double-strand breaks by facilitating the replacement of RPA with RAD51
    Article Snippet: The following antibodies were used in this study: mouse monoclonal anti-SMARCA4 (clone 20C3.2), anti-γH2AX (05-636) and rabbit polyclonal anti-H2A (07-146) (Millipore Corporation, CA); mouse monoclonal anti-HA (H9658), anti-GFP (G1546), anti-actin (A5441) and anti-BrdU (B2531) (Sigma-Aldrich, St Louis, MO); rabbit polyclonal anti-γH2AX (#9718S) (Cell Signaling Technology, Boston, MA); rabbit polyclonal anti-BRG1 (ab70558), anti-NBS1 (ab32074), anti-RAD51 (ab63801) and mouse monoclonal anti-RPA (ab2175) (Abcam, San Francisco, CA); rabbit polyclonal anti-H2AX (10856-1-AP) (Proteintech, Chicago, IL); rabbit polyclonal anti-RAD51 (sc-8349), anti-RAD52 (sc-365341), anti-BRCA2 (sc-28235) and mouse monoclonal anti-p53 (sc-126) (Santa Cruz Biotechnology, Dallas, TX). .. The pBJ5-vector, pBJ5-HA-wtBRG1 and pBJ5-HA-ATPase-mutant BRG1 (K798R) were constructed by Dr Weidong Wang (National Institute on Aging, Baltimore, MD) and Dr Gerald Crabtree (Stanford University, CA) and provided generously by Dr Anthony N. Imbalzano (University of Massachusetts Medical School, MA).

    Real-time Polymerase Chain Reaction:

    Article Title: BRG1 Chromatin Remodeling Activity is Required for Efficient Chromatin Binding by the Transcriptional Repressor Rest and Facilitates Rest-Mediated Repression
    Article Snippet: .. Chromatin from HEK293 cells was precipitated with the anti-BRG1 antibody or IgG and the presence of RE1 sites from five well characterized REST regulated genes ( , , , , ) was quantified by real time PCR. .. BRG1 was enriched at each of the five RE1s under study but not at a control sequence in HEK293 cells ( ).

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA). .. Real-time PCR analysis was performed for BRG1 binding sites in miR-148b promoter by using the following primers: 5′-AGC GCC AGT GTT AAA GGC TA-3′ (forward) and 5′-TCC ATG GGG AAC AGA AGA AG-3′ (reverse).

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]). .. The abundance of specific DNA sequences in the immunoprecipitates was determined by qPCR and corrected for the independently determined amplification curves of each primer set.

    Incubation:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: Membranes were incubated with IRDye secondary antibodies (1:5,000; LI-COR, Inc., Lincoln, NE) for 1 h at room temperature. .. Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Article Title: BRG1 Chromatin Remodeling Activity is Required for Efficient Chromatin Binding by the Transcriptional Repressor Rest and Facilitates Rest-Mediated Repression
    Article Snippet: Extracts were centrifuged for 5 minutes at 13,000 rpm at 4°C and equal aliquots of the supernatant were incubated with a sham antibody (pre-immune serum) or an anti-REST antibody ( ) overnight at 4°C. .. Proteins were eluted in SDS loading buffer along with 10% input and subjected to Western blotting with an anti-BRG1 antibody (Abcam).

    Expressing:

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: Cross-linked chromatin was prepared from ∼2 × 107 cells 48 h after transduction with lentiviruses expressing either hSNF5 or GFP as a control. .. The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]).

    Modification:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: A total of 600 μg of protein was used for immunoprecipitation with protein G beads (GE Healthcare, Little Chalfont, UK) as previously described with some modification ( ). .. Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Western Blot:

    Article Title: Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells
    Article Snippet: .. A300-911A for immunostaining, Abcam ab71153 for IP and western blot), MTA2 (Santa Cruz sc-28731), RBBP4 (Epitomics no. 2599-1), RBBP7 (Epitomics no. 3503-1), LSD1 (Abcam ab17721), BRG1 (Abcam ab4081 for immunostaining, a gift from Dr Wang for IP and western blot), KAP1 (Abcam ab22553), CREST (Antibodies Inc. no. 15-235), DIG (Roche no. 11093274910), and Flag M2 (Sigma F1804). .. Immunofluorescence staining Cells were fixed in 4% paraformaldehyde for 10 min at room temperature and permeabilized in 0.25% NP-40 in phosphate-buffered saline (PBS) for 10 min.

    Article Title: myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis
    Article Snippet: Supernatants were precleared with pre-blocked protein A-agarose (Sigma, P1406) on ice, with agitation for 1 h. Binding reactions were performed with 10 μl anti-Brg1 antibody (see above) or rabbit IgG (Abcam, ab27478) on ice with agitation for 2 h, and for an additional 2 h with 15 μl pre-blocked protein A-agarose. .. Bound immune complexes were washed three times in 20 mM HEPES (pH 7.4), 150 mM NaCl, 10% glycerol, 0.1% Triton X-100 and resuspended in 10 μl 1× Laemmli buffer, boiled and run on 8-12% polyacrylamide gels (Bio-Rad), followed by western blot.

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: Paragraph title: Western blotting and immunoprecipitation ... Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Article Title: Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2–β-Catenin–BRG1 Transcriptional Network-Driven CD47 Expression
    Article Snippet: .. Western blot analysis was performed with protein from whole-cell extracts, cytosolic extracts, and nuclear extracts of cells transfected with different constructs or siRNA and treated with different inhibitors and activators as described previously , using mouse monoclonal anti-CD47 (1:1,000) (catalog no. ab9089; Abcam, Cambridge, UK), rabbit monoclonal anti-PKM2 (1:1,000) (catalog no. 4053; Cell Signaling, Boston, MA), rabbit monoclonal anti-GSK-3β (1:1,000) (catalog no. 9315; Cell Signaling), rabbit polyclonal anti-non-phospho-β-catenin (1:1,000) (catalog no. 4270; Cell Signaling), rabbit monoclonal anti-β-catenin (1:1,000) (catalog no. 8480; Cell Signaling), mouse monoclonal anti-β-catenin (1:1,000) (catalog no. ab19450; Abcam), goat polyclonal anti-TCF4 (1:1,000) (catalog no. sc-8631; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-BRG1 (1:1,000) (catalog no. ab4081; Abcam), mouse monoclonal anti-IDH1-R132H (clone HMab-1; Sigma-Aldrich), and rabbit polyclonal anti-IDH1 (1:1,000) (catalog no. ab135659; Abcam). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Vector Laboratories Inc. (Burlingame, CA).

    Article Title: BRG1 Chromatin Remodeling Activity is Required for Efficient Chromatin Binding by the Transcriptional Repressor Rest and Facilitates Rest-Mediated Repression
    Article Snippet: .. Proteins were eluted in SDS loading buffer along with 10% input and subjected to Western blotting with an anti-BRG1 antibody (Abcam). .. the FLAG epitope-tagged BRG1 DN construct was excised from pBS.CehBRG1K798R (a kind gift from Dr. Tony Imbalzano, University of Massachusetts) using Cla I and Spe I and ligated into the pCS2+ vector, digested with Cla I and Xba I.

    Article Title: SNF5 Is an Essential Executor of Epigenetic Regulation during Differentiation
    Article Snippet: Paragraph title: Western blot analysis ... Antibodies against SNF5 (ab12167), BRG1 (ab4081), BRM (ab15597), and BAF53b (ab103771) were purchased from Abcam, Inc (Cambridge, MA).

    Article Title: BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens
    Article Snippet: Paragraph title: Immunoprecipitation and western blotting ... Immunoprecipitations against Brg1 were performed using 1 μg of antibody ab70558 (Abcam,, Cambridge, MA).

    Transfection:

    Article Title: Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2–β-Catenin–BRG1 Transcriptional Network-Driven CD47 Expression
    Article Snippet: .. Western blot analysis was performed with protein from whole-cell extracts, cytosolic extracts, and nuclear extracts of cells transfected with different constructs or siRNA and treated with different inhibitors and activators as described previously , using mouse monoclonal anti-CD47 (1:1,000) (catalog no. ab9089; Abcam, Cambridge, UK), rabbit monoclonal anti-PKM2 (1:1,000) (catalog no. 4053; Cell Signaling, Boston, MA), rabbit monoclonal anti-GSK-3β (1:1,000) (catalog no. 9315; Cell Signaling), rabbit polyclonal anti-non-phospho-β-catenin (1:1,000) (catalog no. 4270; Cell Signaling), rabbit monoclonal anti-β-catenin (1:1,000) (catalog no. 8480; Cell Signaling), mouse monoclonal anti-β-catenin (1:1,000) (catalog no. ab19450; Abcam), goat polyclonal anti-TCF4 (1:1,000) (catalog no. sc-8631; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-BRG1 (1:1,000) (catalog no. ab4081; Abcam), mouse monoclonal anti-IDH1-R132H (clone HMab-1; Sigma-Aldrich), and rabbit polyclonal anti-IDH1 (1:1,000) (catalog no. ab135659; Abcam). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Vector Laboratories Inc. (Burlingame, CA).

    Article Title: BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens
    Article Snippet: Cells were harvested 48 h after transfection and lysed in TPER (ThermoFisher). .. Immunoprecipitations against Brg1 were performed using 1 μg of antibody ab70558 (Abcam,, Cambridge, MA).

    Immunostaining:

    Article Title: Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells
    Article Snippet: .. A300-911A for immunostaining, Abcam ab71153 for IP and western blot), MTA2 (Santa Cruz sc-28731), RBBP4 (Epitomics no. 2599-1), RBBP7 (Epitomics no. 3503-1), LSD1 (Abcam ab17721), BRG1 (Abcam ab4081 for immunostaining, a gift from Dr Wang for IP and western blot), KAP1 (Abcam ab22553), CREST (Antibodies Inc. no. 15-235), DIG (Roche no. 11093274910), and Flag M2 (Sigma F1804). .. Immunofluorescence staining Cells were fixed in 4% paraformaldehyde for 10 min at room temperature and permeabilized in 0.25% NP-40 in phosphate-buffered saline (PBS) for 10 min.

    Magnetic Beads:

    Article Title: Brg1 modulates enhancer activation in mesoderm lineage commitment
    Article Snippet: Brg1 ChIP-exo was performed as previously described ( ) using anti-Brg1 antibody (Abcam, 110641; 3 µg). .. Briefly, Brg1 ChIP was performed, and, while still on magnetic beads, the immunoprecipitated DNA was polished, ligated with P7 adapter and nicks were repaired.

    other:

    Article Title: Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block
    Article Snippet: Antibodies Following primary antibodies were used in this study: lamin B (Santa Cruz cat. no. sc-6217); filamin (Santa Cruz cat. no. sc-28284); alpha-actinin-4 (Abcam cat. no. ab96866); spectrin (Sigma Aldrich cat. no. S1390); paxillin (Millipore cat. no. 05-471); vinculin (Sigma Aldrich cat. no. V4505); mDia1 (BD Biosciences cat. no. P66520-050); SUN2 (Abcam cat. no. ab124916); emerin (Abcam cat. no. ab40688); Arp3 (Welch et al. ); cofilin (Abcam cat. no. ab11062); P-cofilin (Cell Signaling cat. no. 3313); Arp6 (Sigma Aldrich cat. no. R35554); Arp5 (Kitayama et al. ); Arp8 (Aoyama et al. ); Brg1 (Abcam cat. no. ab70558); hnRNP U (Santa Cruz, clone 3G6); H3K9Me2 (Millipore cat. no. 17-648); H3K4Me2 (Millipore cat. no. 07-030); CTD-phosphoS2 (Abcam cat. no. ab24758); RPA194 (Santa Cruz, cat. no. sc-28714); and BrdU (Sigma Aldrich, clone BU-33).

    Imaging:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: The blots were imaged using the Odyssey Infrared Imaging system (LI-COR, Inc.) and quantified by densitometry using Image J. β-actin was used as a loading control. .. Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Sequencing:

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA. .. To prepare libraries for sequencing, DNA was end repaired, A-tailed, adapter ligated (Illumina TrueSeq) and PCR amplified.

    Sonication:

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: Chromatin was sonicated at output 4 for 30 s twice with a 1 min pause between cycles then centrifuged at 10,000 g for 10 min at 4°C and stored at −80°C. .. Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA.

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: Chromatin was prepared according to standard protocols and sheared to fragments in a Bioruptor (Diagenode, Philadelphia, PA, USA) by three sonication cycles of 5 minutes. .. The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA).

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: Chromatin isolation, sonication yielding fragments of 300 to 600 bp, and immunoprecipitations were performed according to previously established protocols. .. The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]).

    Injection:

    Article Title: myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis
    Article Snippet: Somites from embryos injected at HH14-15 with antagomirs were dissected after 24 h, pooled and lysed in 20 mM HEPES (pH 7.4), 150 mM NaCl, 10% glycerol, 1% Triton X-100 on ice for 15 min. We obtained 20 μg protein from untreated somites and 10 μg from antagomir-injected somites. .. Supernatants were precleared with pre-blocked protein A-agarose (Sigma, P1406) on ice, with agitation for 1 h. Binding reactions were performed with 10 μl anti-Brg1 antibody (see above) or rabbit IgG (Abcam, ab27478) on ice with agitation for 2 h, and for an additional 2 h with 15 μl pre-blocked protein A-agarose.

    Binding Assay:

    Article Title: myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis
    Article Snippet: .. Supernatants were precleared with pre-blocked protein A-agarose (Sigma, P1406) on ice, with agitation for 1 h. Binding reactions were performed with 10 μl anti-Brg1 antibody (see above) or rabbit IgG (Abcam, ab27478) on ice with agitation for 2 h, and for an additional 2 h with 15 μl pre-blocked protein A-agarose. .. Bound immune complexes were washed three times in 20 mM HEPES (pH 7.4), 150 mM NaCl, 10% glycerol, 0.1% Triton X-100 and resuspended in 10 μl 1× Laemmli buffer, boiled and run on 8-12% polyacrylamide gels (Bio-Rad), followed by western blot.

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA). .. Real-time PCR analysis was performed for BRG1 binding sites in miR-148b promoter by using the following primers: 5′-AGC GCC AGT GTT AAA GGC TA-3′ (forward) and 5′-TCC ATG GGG AAC AGA AGA AG-3′ (reverse).

    ChIP-sequencing:

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: Paragraph title: ChIP-Seq ... Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA.

    Article Title: Brg1 modulates enhancer activation in mesoderm lineage commitment
    Article Snippet: ChIP-seq analysis pipeline and statistical methods are provided in the supplementary material Methods . .. Brg1 ChIP-exo was performed as previously described ( ) using anti-Brg1 antibody (Abcam, 110641; 3 µg).

    Methylation:

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]). .. For methylated DNA immunoprecipitate (MeDIP) assays, genomic DNA was prepared by overnight proteinase K treatment, phenol-chloroform extraction, and ethanol precipitation.

    Isolation:

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: Chromatin isolation, sonication yielding fragments of 300 to 600 bp, and immunoprecipitations were performed according to previously established protocols. .. The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]).

    Purification:

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA. .. Samples were reverse crosslinked, digested with proteinase K and RNAse A, and purified using AMPure XP beads (Beckman Coulter).

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA). .. Precipitated DNA fragments were purified by using PCR purification kit (Qiagen, Valencia, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA. .. To prepare libraries for sequencing, DNA was end repaired, A-tailed, adapter ligated (Illumina TrueSeq) and PCR amplified.

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA). .. Precipitated DNA fragments were purified by using PCR purification kit (Qiagen, Valencia, CA, USA).

    Blocking Assay:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: Membranes were incubated overnight at 4°C with primary antibodies diluted in blocking buffer (Rockland Immunochemicals, Inc., Limerick, PA), including: rabbit anti-BRG1 (1:2,000; Abcam, Cambridge, MA), rabbit anti-phospho(p)-SMAD3 (1:500; Calbiochem of Millipore Corp., Billerica, MA), and mouse anti-β-actin (1:10,000; Cell Signaling Technologies, Inc., Danvers, MA). .. Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Chromatin Immunoprecipitation:

    Article Title: Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells
    Article Snippet: Antibodies The following antibodies were used: mouse polyclonal anti-Zscan4 (Abnova H00201516- B01P), rabbit polyclonal anti-Zscan4, HP1α (Millipore MAB3584 for immunostaining, Millipore no. 05-689 for immunoblotting), H3K9me3 (Upstate no. 07-442), H4K20me3 (Millipore no. 07-463), H3K4me3 (Millipore no. 07-473), H3K9ac (Millipore no. 07-352), H3K14ac (Millipore no. 17-10051), H3K18ac (Epitomics no. 1766-1), H3K27ac (Active Motif no. 39-133 for immunostaining and ChIP, Abcam ab4729 for immunostaining and immunoblotting), H3K9me2 (Abcam ab1220), pan-H3 (Abcam ab1791), p300 (Millipore no. 05-257), CBP (Santa Cruz sc-583), HDAC1 (Abcam ab7028 for immunostaining, ab31263 for western blot), HDAC2 (ab7029), MTA1 (Bethyl lab. .. A300-911A for immunostaining, Abcam ab71153 for IP and western blot), MTA2 (Santa Cruz sc-28731), RBBP4 (Epitomics no. 2599-1), RBBP7 (Epitomics no. 3503-1), LSD1 (Abcam ab17721), BRG1 (Abcam ab4081 for immunostaining, a gift from Dr Wang for IP and western blot), KAP1 (Abcam ab22553), CREST (Antibodies Inc. no. 15-235), DIG (Roche no. 11093274910), and Flag M2 (Sigma F1804).

    Article Title: Brg1 modulates enhancer activation in mesoderm lineage commitment
    Article Snippet: .. Brg1 ChIP-exo was performed as previously described ( ) using anti-Brg1 antibody (Abcam, 110641; 3 µg). .. Briefly, Brg1 ChIP was performed, and, while still on magnetic beads, the immunoprecipitated DNA was polished, ligated with P7 adapter and nicks were repaired.

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: .. To determine whether BRG1 binds to miR-148b promoter, ChIP assay was conducted using anti-BRG1 antibody in A549 cells. .. As shown in , BRG1 showed a high capacity to bind to the promoter region of miR-148b compared with control IgG.

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: Paragraph title: Chromatin immunoprecipitation assay ... The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA).

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: Paragraph title: ChIP and MeDIP assays. ... The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]).

    SDS Page:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C. .. After the final wash, reducing sample buffer containing SDS and DTT was added to the pelleted beads and samples were heated to 95°C for 5 min and subjected to SDS-PAGE.

    Article Title: SNF5 Is an Essential Executor of Epigenetic Regulation during Differentiation
    Article Snippet: Western blot analysis Cell lysates were boiled in Laemmli sample buffer for 3 min, and 30 µg of each protein were subjected to SDS-PAGE. .. Antibodies against SNF5 (ab12167), BRG1 (ab4081), BRM (ab15597), and BAF53b (ab103771) were purchased from Abcam, Inc (Cambridge, MA).

    Plasmid Preparation:

    Article Title: Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2–β-Catenin–BRG1 Transcriptional Network-Driven CD47 Expression
    Article Snippet: Western blot analysis was performed with protein from whole-cell extracts, cytosolic extracts, and nuclear extracts of cells transfected with different constructs or siRNA and treated with different inhibitors and activators as described previously , using mouse monoclonal anti-CD47 (1:1,000) (catalog no. ab9089; Abcam, Cambridge, UK), rabbit monoclonal anti-PKM2 (1:1,000) (catalog no. 4053; Cell Signaling, Boston, MA), rabbit monoclonal anti-GSK-3β (1:1,000) (catalog no. 9315; Cell Signaling), rabbit polyclonal anti-non-phospho-β-catenin (1:1,000) (catalog no. 4270; Cell Signaling), rabbit monoclonal anti-β-catenin (1:1,000) (catalog no. 8480; Cell Signaling), mouse monoclonal anti-β-catenin (1:1,000) (catalog no. ab19450; Abcam), goat polyclonal anti-TCF4 (1:1,000) (catalog no. sc-8631; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-BRG1 (1:1,000) (catalog no. ab4081; Abcam), mouse monoclonal anti-IDH1-R132H (clone HMab-1; Sigma-Aldrich), and rabbit polyclonal anti-IDH1 (1:1,000) (catalog no. ab135659; Abcam). .. Western blot analysis was performed with protein from whole-cell extracts, cytosolic extracts, and nuclear extracts of cells transfected with different constructs or siRNA and treated with different inhibitors and activators as described previously , using mouse monoclonal anti-CD47 (1:1,000) (catalog no. ab9089; Abcam, Cambridge, UK), rabbit monoclonal anti-PKM2 (1:1,000) (catalog no. 4053; Cell Signaling, Boston, MA), rabbit monoclonal anti-GSK-3β (1:1,000) (catalog no. 9315; Cell Signaling), rabbit polyclonal anti-non-phospho-β-catenin (1:1,000) (catalog no. 4270; Cell Signaling), rabbit monoclonal anti-β-catenin (1:1,000) (catalog no. 8480; Cell Signaling), mouse monoclonal anti-β-catenin (1:1,000) (catalog no. ab19450; Abcam), goat polyclonal anti-TCF4 (1:1,000) (catalog no. sc-8631; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-BRG1 (1:1,000) (catalog no. ab4081; Abcam), mouse monoclonal anti-IDH1-R132H (clone HMab-1; Sigma-Aldrich), and rabbit polyclonal anti-IDH1 (1:1,000) (catalog no. ab135659; Abcam).

    Article Title: BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens
    Article Snippet: HT22 cells (Salk Institute, La Jolla, CA) were transfected with either the empty vector, pLVX-EF1a-IRES-mCherry, MW95 or MW97 using Lipofectamine LTX with Plus Reagent (ThermoFisher, Waltham, MA). .. Immunoprecipitations against Brg1 were performed using 1 μg of antibody ab70558 (Abcam,, Cambridge, MA).

    Article Title: BRG1 promotes the repair of DNA double-strand breaks by facilitating the replacement of RPA with RAD51
    Article Snippet: The following antibodies were used in this study: mouse monoclonal anti-SMARCA4 (clone 20C3.2), anti-γH2AX (05-636) and rabbit polyclonal anti-H2A (07-146) (Millipore Corporation, CA); mouse monoclonal anti-HA (H9658), anti-GFP (G1546), anti-actin (A5441) and anti-BrdU (B2531) (Sigma-Aldrich, St Louis, MO); rabbit polyclonal anti-γH2AX (#9718S) (Cell Signaling Technology, Boston, MA); rabbit polyclonal anti-BRG1 (ab70558), anti-NBS1 (ab32074), anti-RAD51 (ab63801) and mouse monoclonal anti-RPA (ab2175) (Abcam, San Francisco, CA); rabbit polyclonal anti-H2AX (10856-1-AP) (Proteintech, Chicago, IL); rabbit polyclonal anti-RAD51 (sc-8349), anti-RAD52 (sc-365341), anti-BRCA2 (sc-28235) and mouse monoclonal anti-p53 (sc-126) (Santa Cruz Biotechnology, Dallas, TX). .. The pBABE HA- Asi SI-ER plasmid was provided generously by Dr Gaelle Legube (Laboratoire de Biologie Cellulaire et Moleculaire du Controle de la Proliferation, France).

    Software:

    Article Title: Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2–β-Catenin–BRG1 Transcriptional Network-Driven CD47 Expression
    Article Snippet: Western blot analysis was performed with protein from whole-cell extracts, cytosolic extracts, and nuclear extracts of cells transfected with different constructs or siRNA and treated with different inhibitors and activators as described previously , using mouse monoclonal anti-CD47 (1:1,000) (catalog no. ab9089; Abcam, Cambridge, UK), rabbit monoclonal anti-PKM2 (1:1,000) (catalog no. 4053; Cell Signaling, Boston, MA), rabbit monoclonal anti-GSK-3β (1:1,000) (catalog no. 9315; Cell Signaling), rabbit polyclonal anti-non-phospho-β-catenin (1:1,000) (catalog no. 4270; Cell Signaling), rabbit monoclonal anti-β-catenin (1:1,000) (catalog no. 8480; Cell Signaling), mouse monoclonal anti-β-catenin (1:1,000) (catalog no. ab19450; Abcam), goat polyclonal anti-TCF4 (1:1,000) (catalog no. sc-8631; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-BRG1 (1:1,000) (catalog no. ab4081; Abcam), mouse monoclonal anti-IDH1-R132H (clone HMab-1; Sigma-Aldrich), and rabbit polyclonal anti-IDH1 (1:1,000) (catalog no. ab135659; Abcam). .. After the addition of a chemiluminescent reagent (Millipore, Billerica, MA), blots were exposed to the Chemigenius Bioimaging system (Syngene, Cambridge, UK), and images were captured with Gene Snap software (Syngene).

    Homogenization:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: For immunoprecipitation, NAc or CPU tissue punches from rats ( n = 8 for the saline group, n = 7 for the cocaine group) were homogenized in 500 μL of homogenization buffer containing 50 mM beta-glycerol phosphate, 1 mM DTT, 2 mM EGTA, 10 mM NaF, 1 mM sodium orthovanadate, 20 mM HEPES (pH 7.4), 1% Triton X-100, 10% glycerol, 0.5% SDS, phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO), and protease inhibitors (Roche, Basel, Switzerland). .. Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Ethanol Precipitation:

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]). .. For methylated DNA immunoprecipitate (MeDIP) assays, genomic DNA was prepared by overnight proteinase K treatment, phenol-chloroform extraction, and ethanol precipitation.

    Immunoprecipitation:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: Paragraph title: Western blotting and immunoprecipitation ... Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C.

    Article Title: Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis
    Article Snippet: .. Chromatin (40 μg) was diluted to fivefold, pre-cleared for 2 h followed by immunoprecipitation with anti-Brg1 antibody (Abcam, 110641) for 12-16 h. 5% of samples were used as input DNA. .. Antibody-bound BRG1-DNA complexes were immunoprecipitated using 25 μl of M-280 goat anti-rabbit IgG dyna beads for 2 h, washed a total of ten times with buffers [twice with IP wash buffer 1 containing 50 mM Tris.Cl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate and 1 mM EDTA), five times with IP wash buffer 2 containing 100 mM Tris.Cl (pH 9.0), 500 mM LiCl, 1% NP-40 and 1% sodium deoxcholate, and then twice with IP wash buffer 2 along with 150 mM NaCl] of increasing stringency and eluted with 200 μl of elution buffer [10 mM TrisCl (pH 7.5), 1 mM EDTA and 1%SDS).

    Article Title: Brg1 modulates enhancer activation in mesoderm lineage commitment
    Article Snippet: Brg1 ChIP-exo was performed as previously described ( ) using anti-Brg1 antibody (Abcam, 110641; 3 µg). .. Briefly, Brg1 ChIP was performed, and, while still on magnetic beads, the immunoprecipitated DNA was polished, ligated with P7 adapter and nicks were repaired.

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: .. The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA). .. After extensive washing and elution, crosslinks were reverted by adjusting NaCl concentrations and heat treatment.

    Article Title: BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens
    Article Snippet: Paragraph title: Immunoprecipitation and western blotting ... Immunoprecipitations against Brg1 were performed using 1 μg of antibody ab70558 (Abcam,, Cambridge, MA).

    Methylated DNA Immunoprecipitation:

    Article Title: SWI/SNF Mediates Polycomb Eviction and Epigenetic Reprogramming of the INK4b-ARF-INK4a Locus
    Article Snippet: Paragraph title: ChIP and MeDIP assays. ... The following antibodies were used: SNF5 (ab12167 [Abcam]), BRG1 (ab4081 [Abcam]), SUZ12 (ab12073 [Abcam]), BMI1 (ab14389 [Abcam]), EZH2 (Sc-25383 [Santa Cruz]), DNMT3B (IMG-184A [IMGENEX]), POL II (Sc-899 [Santa Cruz]), mixed lineage leukemia 1 (MLL1; A300-086A [Bethyl Laboratories]), Histone H3 (ab1791 [Abcam]), H3-K4me3 (ab12209 [Abcam]), and H3-K27me3 (catalog no. 07-449 [Upstate]).

    Lysis:

    Article Title: BRG1 in the nucleus accumbens regulates cocaine-seeking behavior
    Article Snippet: .. Briefly, samples were brought to a final volume of 500 μL with lysis buffer, and 5 μL of anti-BRG1 antibody (Abcam) was added followed by gentle tumbling overnight at 4°C. ..

    Article Title: Restoration of BRG1 inhibits proliferation and metastasis of lung cancer by regulating tumor suppressor miR-148b
    Article Snippet: A549 cells were treated with 10% formaldehyde for 10 minutes at room temperature, washed with phosphate buffered saline (PBS), and resuspended in lysis buffer (50 mM Tris-HCl pH 8.0, 40 mM ethylenediaminetetraacetic acid (EDTA), 0.5% SDS). .. The sheared chromatin was then immunoprecipitated by using anti-BRG1 antibody (Abcam, Cambridge, UK) or control IgG (Sigma, St. Louis, MO, USA).

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    Abcam smarca4
    SIRT6 mediates HO-1 transcriptional enhancement through mono-ADP ribosylation of BAF170. ( A ) SIRT6 is required for recruitment of BAF complex to E1 region. ChIP analysis of the HO-1 locus in WT and SIRT6 KO cells untreated and post paraquat treatment using antibody against BAF complex ATPase subunit <t>BRG1.</t> Error bars denote s.d., n = 3; * P
    Smarca4, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smarca4/product/Abcam
    Average 90 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    smarca4 - by Bioz Stars, 2020-01
    90/100 stars
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    90
    Abcam antibody ab70558
    SIRT6 mediates HO-1 transcriptional enhancement through mono-ADP ribosylation of BAF170. ( A ) SIRT6 is required for recruitment of BAF complex to E1 region. ChIP analysis of the HO-1 locus in WT and SIRT6 KO cells untreated and post paraquat treatment using antibody against BAF complex ATPase subunit <t>BRG1.</t> Error bars denote s.d., n = 3; * P
    Antibody Ab70558, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody ab70558/product/Abcam
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody ab70558 - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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    SIRT6 mediates HO-1 transcriptional enhancement through mono-ADP ribosylation of BAF170. ( A ) SIRT6 is required for recruitment of BAF complex to E1 region. ChIP analysis of the HO-1 locus in WT and SIRT6 KO cells untreated and post paraquat treatment using antibody against BAF complex ATPase subunit BRG1. Error bars denote s.d., n = 3; * P

    Journal: Nucleic Acids Research

    Article Title: SIRT6 promotes transcription of a subset of NRF2 targets by mono-ADP-ribosylating BAF170

    doi: 10.1093/nar/gkz528

    Figure Lengend Snippet: SIRT6 mediates HO-1 transcriptional enhancement through mono-ADP ribosylation of BAF170. ( A ) SIRT6 is required for recruitment of BAF complex to E1 region. ChIP analysis of the HO-1 locus in WT and SIRT6 KO cells untreated and post paraquat treatment using antibody against BAF complex ATPase subunit BRG1. Error bars denote s.d., n = 3; * P

    Article Snippet: The following antibodies were used in this study: anti-RNA polymerase II CTD (ab5131), anti-Nrf2 (ab62352), anti BRG1 (ab110641), anti-H3 tri methyl (ab8580), anti-SIRT6 (ab62739 and ab191385) from Abcam.

    Techniques: Chromatin Immunoprecipitation