Structured Review

Becton Dickinson brefeldin a bfa
Brefeldin A Bfa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brefeldin a bfa/product/Becton Dickinson
Average 92 stars, based on 25 article reviews
Price from $9.99 to $1999.99
brefeldin a bfa - by Bioz Stars, 2020-11
92/100 stars

Images

Related Articles

Staining:

Article Title: Inferior immunogenicity and efficacy of respiratory syncytial virus fusion protein-based subunit vaccine candidates in aged versus young mice
Article Snippet: .. For intracellular cytokine staining (ICS) cells were incubated with 1 μg/mL of RSV F overlapping peptide pool (JPT, Berlin, Germany) for 12 hours in the presence of 10 μg/mL of brefeldin A (BFA) (BD Biosciences) at 37°C. .. Following stimulation, cells were blocked with CD16/32 (BD Biosciences) and stained with Fixable Live/Dead dye (ThemoFisher) for 20 min.

Article Title: A Novel IL-17 Dependent Mechanism of Cross Protection: Respiratory Infection with Mycoplasma Protects Against a Secondary Listeria Infection
Article Snippet: .. To accomplish intracellular cytokine staining, GolgiPlug containing Brefeldin A (BFA) (BD PharMingen), was added 4 hrs prior to the harvest of the cell cultures. .. Staining of the cells involved incubation at 4°C for 15 min in staining buffer (PBS + 2% FCS + 0.1% sodium azide) with saturating amounts of the cell-surface antibodies and anti-CD16/CD32 to block Fc receptors.

Concentration Assay:

Article Title: DOPS Adjuvant Confers Enhanced Protection against Malaria for VLP-TRAP Based Vaccines
Article Snippet: .. The PBMC cells were stimulated with immunodominant Pb9 peptide for 12 h with a concentration of 1 µg/ml [ ], as well as brefeldin A (BFA) (BD GolgiPlug™). .. To check the cell viability, aqua live/dead cell staining (Thermo Fisher Scientific) was added.

Incubation:

Article Title: Inferior immunogenicity and efficacy of respiratory syncytial virus fusion protein-based subunit vaccine candidates in aged versus young mice
Article Snippet: .. For intracellular cytokine staining (ICS) cells were incubated with 1 μg/mL of RSV F overlapping peptide pool (JPT, Berlin, Germany) for 12 hours in the presence of 10 μg/mL of brefeldin A (BFA) (BD Biosciences) at 37°C. .. Following stimulation, cells were blocked with CD16/32 (BD Biosciences) and stained with Fixable Live/Dead dye (ThemoFisher) for 20 min.

Article Title: Cell Surface Delivery of the Measles Virus Nucleoprotein: a Viral Strategy To Induce Immunosuppression †
Article Snippet: .. For transwell experiments, 2 × 105 P815-M and P815-N cells separated by a 0.4-μm-pore-size membrane (Costar, Corning, N.Y.) were incubated for 48 h. Treatment with brefeldin A (BFA) and monensin (BD Bioscience, San Diego, Calif.) was performed according to the manufacturer's instructions. .. Wild-type MV strains G954, G945, and G943 ( ) were propagated on PBLs.

Article Title: Peripheral lymph nodes contain migratory and resident innate lymphoid cell populations
Article Snippet: .. In AS01 experiments, assessing the production of IFN-γ, cell suspensions were incubated for 3h in culture media in the presence of brefeldin A (BFA) (10 μg/ml, BD Biosciences) and monensin (10 μg/ml, BD Biosciences) at 37°C 5% C02 . ..

Negative Control:

Article Title: Identification of Three Novel Superantigen-Encoding Genes in Streptococcus equi subsp. zooepidemicus, szeF, szeN, and szeP ▿ ▿ †
Article Snippet: .. For measurement of IFN-γ synthesis, 1 × 106 fresh PBMCs were stimulated in vitro with sAgs at the indicated concentrations, or medium alone as a negative control, in the presence of brefeldin A (BFA) (BD GolgiPlug; BD Biosciences). .. After overnight incubation, the cells were fixed in 3% (vol/vol) formaldehyde in PBS prior to detection of equine IFN-γ by intracellular-cytokine staining (ICC) using an antibody specific for equine IFN-γ (clone CC302; Serotec) and fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG antibody (Jackson ImmunoResearch).

In Vitro:

Article Title: Identification of Three Novel Superantigen-Encoding Genes in Streptococcus equi subsp. zooepidemicus, szeF, szeN, and szeP ▿ ▿ †
Article Snippet: .. For measurement of IFN-γ synthesis, 1 × 106 fresh PBMCs were stimulated in vitro with sAgs at the indicated concentrations, or medium alone as a negative control, in the presence of brefeldin A (BFA) (BD GolgiPlug; BD Biosciences). .. After overnight incubation, the cells were fixed in 3% (vol/vol) formaldehyde in PBS prior to detection of equine IFN-γ by intracellular-cytokine staining (ICC) using an antibody specific for equine IFN-γ (clone CC302; Serotec) and fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG antibody (Jackson ImmunoResearch).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Becton Dickinson brefeldin a
    TLR agonists induce IL-12 and COX-2 in mDC in the absence of costimulatory cytokines PBMC were incubated for 20 hours with inactivated HIV-1 or synthetic TLR agonists in the presence of 5 ug/ml the protein transport inhibitor <t>Brefeldin</t> A (BFA). Intracellular accumulation of IFN-α was measured. (a ) Representative flow cytometric analysis of the selective detection of intracellular IL-12p40/p70 or COX-2 in mDC; [logical gating was used to identify the mDC population from 1 HIV infected individual (Lin1-/HLA DR+/CD11c+)] following stimulation. ( b ) 12 HIV + and 10 uninfected individuals were evaluated for intracellular accumulation of IL-12 in mDC following stimulation with TLR4, TLR7, TLR8, TLR7/8 and TLR9 agonists in the presence BFA. TLR4 was included as a negative control and TLR9 was included as a positive control. ( c ) The same individuals were evaluated for intracellular accumulation of COX-2 in mDC. Results in b and c are expressed as the mean ± SEM percentage of mDC expressing the intracellular marker. Statistical comparisons were made between the media control and the corresponding TLR agonist using a Student’s t-test; values were considered significant if p
    Brefeldin A, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Becton Dickinson
    Average 94 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    92
    Becton Dickinson brefeldin a bfa
    TLR agonists induce IL-12 and COX-2 in mDC in the absence of costimulatory cytokines PBMC were incubated for 20 hours with inactivated HIV-1 or synthetic TLR agonists in the presence of 5 ug/ml the protein transport inhibitor <t>Brefeldin</t> A (BFA). Intracellular accumulation of IFN-α was measured. (a ) Representative flow cytometric analysis of the selective detection of intracellular IL-12p40/p70 or COX-2 in mDC; [logical gating was used to identify the mDC population from 1 HIV infected individual (Lin1-/HLA DR+/CD11c+)] following stimulation. ( b ) 12 HIV + and 10 uninfected individuals were evaluated for intracellular accumulation of IL-12 in mDC following stimulation with TLR4, TLR7, TLR8, TLR7/8 and TLR9 agonists in the presence BFA. TLR4 was included as a negative control and TLR9 was included as a positive control. ( c ) The same individuals were evaluated for intracellular accumulation of COX-2 in mDC. Results in b and c are expressed as the mean ± SEM percentage of mDC expressing the intracellular marker. Statistical comparisons were made between the media control and the corresponding TLR agonist using a Student’s t-test; values were considered significant if p
    Brefeldin A Bfa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a bfa/product/Becton Dickinson
    Average 92 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    brefeldin a bfa - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    Image Search Results


    TLR agonists induce IL-12 and COX-2 in mDC in the absence of costimulatory cytokines PBMC were incubated for 20 hours with inactivated HIV-1 or synthetic TLR agonists in the presence of 5 ug/ml the protein transport inhibitor Brefeldin A (BFA). Intracellular accumulation of IFN-α was measured. (a ) Representative flow cytometric analysis of the selective detection of intracellular IL-12p40/p70 or COX-2 in mDC; [logical gating was used to identify the mDC population from 1 HIV infected individual (Lin1-/HLA DR+/CD11c+)] following stimulation. ( b ) 12 HIV + and 10 uninfected individuals were evaluated for intracellular accumulation of IL-12 in mDC following stimulation with TLR4, TLR7, TLR8, TLR7/8 and TLR9 agonists in the presence BFA. TLR4 was included as a negative control and TLR9 was included as a positive control. ( c ) The same individuals were evaluated for intracellular accumulation of COX-2 in mDC. Results in b and c are expressed as the mean ± SEM percentage of mDC expressing the intracellular marker. Statistical comparisons were made between the media control and the corresponding TLR agonist using a Student’s t-test; values were considered significant if p

    Journal: Cellular immunology

    Article Title: Dendritic Cells from HIV-1 Infected Individuals are Less Responsive to Toll-like Receptor (TLR) Ligands

    doi: 10.1016/j.cellimm.2008.01.007

    Figure Lengend Snippet: TLR agonists induce IL-12 and COX-2 in mDC in the absence of costimulatory cytokines PBMC were incubated for 20 hours with inactivated HIV-1 or synthetic TLR agonists in the presence of 5 ug/ml the protein transport inhibitor Brefeldin A (BFA). Intracellular accumulation of IFN-α was measured. (a ) Representative flow cytometric analysis of the selective detection of intracellular IL-12p40/p70 or COX-2 in mDC; [logical gating was used to identify the mDC population from 1 HIV infected individual (Lin1-/HLA DR+/CD11c+)] following stimulation. ( b ) 12 HIV + and 10 uninfected individuals were evaluated for intracellular accumulation of IL-12 in mDC following stimulation with TLR4, TLR7, TLR8, TLR7/8 and TLR9 agonists in the presence BFA. TLR4 was included as a negative control and TLR9 was included as a positive control. ( c ) The same individuals were evaluated for intracellular accumulation of COX-2 in mDC. Results in b and c are expressed as the mean ± SEM percentage of mDC expressing the intracellular marker. Statistical comparisons were made between the media control and the corresponding TLR agonist using a Student’s t-test; values were considered significant if p

    Article Snippet: The protein transport inhibitor, Brefeldin A (BFA; 5 μg/ml; BD), was added at the beginning of the culture period (Time = 0 hours) to cultures containing Media, TLR7, TLR8, TLR7/8 and control TLR7/8 agonists or 2 hours following the addition of the TLR9 agonist (CpG ODN).

    Techniques: Incubation, Flow Cytometry, Infection, Negative Control, Positive Control, Expressing, Marker, IF-P

    HIV-1 and TLR agonists activate IFN-α production in pDC in the absence of costimulatory cytokines PBMC were incubated for 20 hours with inactivated HIV-1 or synthetic TLR agonists in the presence of 5 ug/ml the protein transport inhibitor Brefeldin A (BFA). Intracellular accumulation of IFN-α was measured. (a ) Representative flow cytometric analysis of the selective detection of intracellular IFN-α in pDC [logical gating was used to identify the pDC population from 1 HIV-1 infected individual (Lin1 − /HLA DR + /CD123 + )] following stimulation. ( b ) 12 HIV + and 10 HIV − individuals were evaluated for intracellular accumulation of IFN-α following stimulation with TLR4, TLR7, TLR8, TLR7/8 and TLR9 agonists in the presence BFA. TLR4 was included as a negative control and TLR9 was included as a positive control. ( c ) 4 HIV + and 10 HIV − individuals were evaluated for intracellular accumulation of IFN-α following stimulation with AT-2 inactivated HIV-1 Ada (R5-tropic), AT-2 inactivated HIV-1 MN (X4-tropic) and matched control microvesicles (MV-CCR5, MV-CEMX) in the presence of BFA. Results are expressed as the mean ± SEM of the percentage of pDC that expresses intracellular IFN-α. Statistical comparisons were made between the media control and the corresponding TLR agonist or AT-2 inactivated HIV-1 virus using a Student’s t-test; values were considered significant if p

    Journal: Cellular immunology

    Article Title: Dendritic Cells from HIV-1 Infected Individuals are Less Responsive to Toll-like Receptor (TLR) Ligands

    doi: 10.1016/j.cellimm.2008.01.007

    Figure Lengend Snippet: HIV-1 and TLR agonists activate IFN-α production in pDC in the absence of costimulatory cytokines PBMC were incubated for 20 hours with inactivated HIV-1 or synthetic TLR agonists in the presence of 5 ug/ml the protein transport inhibitor Brefeldin A (BFA). Intracellular accumulation of IFN-α was measured. (a ) Representative flow cytometric analysis of the selective detection of intracellular IFN-α in pDC [logical gating was used to identify the pDC population from 1 HIV-1 infected individual (Lin1 − /HLA DR + /CD123 + )] following stimulation. ( b ) 12 HIV + and 10 HIV − individuals were evaluated for intracellular accumulation of IFN-α following stimulation with TLR4, TLR7, TLR8, TLR7/8 and TLR9 agonists in the presence BFA. TLR4 was included as a negative control and TLR9 was included as a positive control. ( c ) 4 HIV + and 10 HIV − individuals were evaluated for intracellular accumulation of IFN-α following stimulation with AT-2 inactivated HIV-1 Ada (R5-tropic), AT-2 inactivated HIV-1 MN (X4-tropic) and matched control microvesicles (MV-CCR5, MV-CEMX) in the presence of BFA. Results are expressed as the mean ± SEM of the percentage of pDC that expresses intracellular IFN-α. Statistical comparisons were made between the media control and the corresponding TLR agonist or AT-2 inactivated HIV-1 virus using a Student’s t-test; values were considered significant if p

    Article Snippet: The protein transport inhibitor, Brefeldin A (BFA; 5 μg/ml; BD), was added at the beginning of the culture period (Time = 0 hours) to cultures containing Media, TLR7, TLR8, TLR7/8 and control TLR7/8 agonists or 2 hours following the addition of the TLR9 agonist (CpG ODN).

    Techniques: Incubation, Flow Cytometry, Infection, Negative Control, Positive Control, IF-P