breast epithelial cells  (ATCC)


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    Structured Review

    ATCC breast epithelial cells
    Breast Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 11 article reviews
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    breast epithelial cells - by Bioz Stars, 2022-11
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    ATCC human breast normal epithelial cell mcf 10 a
    TS inhibited the proliferation and invasion of MDA-MB-231 and BT-549 <t>cells</t> and the suppression by CAXII knockout. The relative CAXII expression in MCF-10 A was significantly lower than that of MDA-MB-231 and BT-549, respectively (P
    Human Breast Normal Epithelial Cell Mcf 10 A, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC mammary epithelial cell line mcf10a
    Aerotaxis of breast cancer cells is dependent on EGFR activation. (A) Immunofluorescence imaging of EGFR in <t>MCF10A</t> cells in the absence of EGF (-EGF), upon activation by EGF (+ EGF), or EGF + cetuximab, a monoclonal EGFR neutralising antibody (+ EGF + Cetux), (B) Immunofluorescence imaging of EGFR in the indicated tumour cells in their culture medium supplemented with EGF. Scale bars, 25 μm and 10 μm for high magnification views inserted in the upper-right corner of each image (x2.5). Steady-state EGFR (-EGF and + EGF/+Cetux conditions) is located at the plasma membrane, whereas it appears as intracellular dots upon activation (+ EGF condition). (C) Aerotactic migration at 48 h of the indicated tumour cells either untreated (Mock), treated with 25 µg/mL or with 12.5 µg/mL cetuximab (Cetux). Scale bars 0.5 mm. (D) <t>Cell</t> distribution from the centre of the cell cluster (in mm) at 48 h in the Mock condition (carmine, mean of three independent experiments), in cells treated with 25 µg/mL (blue) or with 12.5 µg/mL (yellow). Y-axis scale is expressed in arbitrary unit. See Figure S 5 for the other tumour cells
    Mammary Epithelial Cell Line Mcf10a, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    ATCC t47d cells
    Aerotaxis of breast cancer cells is dependent on EGFR activation. (A) Immunofluorescence imaging of EGFR in <t>MCF10A</t> cells in the absence of EGF (-EGF), upon activation by EGF (+ EGF), or EGF + cetuximab, a monoclonal EGFR neutralising antibody (+ EGF + Cetux), (B) Immunofluorescence imaging of EGFR in the indicated tumour cells in their culture medium supplemented with EGF. Scale bars, 25 μm and 10 μm for high magnification views inserted in the upper-right corner of each image (x2.5). Steady-state EGFR (-EGF and + EGF/+Cetux conditions) is located at the plasma membrane, whereas it appears as intracellular dots upon activation (+ EGF condition). (C) Aerotactic migration at 48 h of the indicated tumour cells either untreated (Mock), treated with 25 µg/mL or with 12.5 µg/mL cetuximab (Cetux). Scale bars 0.5 mm. (D) <t>Cell</t> distribution from the centre of the cell cluster (in mm) at 48 h in the Mock condition (carmine, mean of three independent experiments), in cells treated with 25 µg/mL (blue) or with 12.5 µg/mL (yellow). Y-axis scale is expressed in arbitrary unit. See Figure S 5 for the other tumour cells
    T47d Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC hc11 mammary epithelium
    Interferon γ-mediated autophagy contributes to the production of ADMA. 4T1 breast cancer cells were treated with low dose (0.2 ng/mL) or high dose (200 ng/mL) IFN-γ for 3 days. Induction of autophagy was measured using Premo LC3B-RFP viral transduction. The high dose of IFN-γ induced autophagy of 4T1 cells ( A ). The same dose of IFN-γ also led to increased secretion of ADMA into the conditioned medium in mouse breast cancer 4T1 cells ( B ) and IFN-γ induced ADMA secretion was blocked in 4T1 cells when 250 nM autophagy inhibitor was added to the culture ( B ). The effect of IFN-γ on the EMT 6 ( C ) or mouse normal <t>mammary</t> epithelial cell line, <t>HC11,</t> was not significant and unaffected by the autophagy inhibitor ( D ). ADMA concentration was expressed as ng/mL (left Y axis) or µM (right Y axis). Data are presented as mean ± SEM. (*p
    Hc11 Mammary Epithelium, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TS inhibited the proliferation and invasion of MDA-MB-231 and BT-549 cells and the suppression by CAXII knockout. The relative CAXII expression in MCF-10 A was significantly lower than that of MDA-MB-231 and BT-549, respectively (P

    Journal: Cancer Cell International

    Article Title: Tiliroside suppresses triple-negative breast cancer as a multifunctional CAXII inhibitor

    doi: 10.1186/s12935-022-02786-6

    Figure Lengend Snippet: TS inhibited the proliferation and invasion of MDA-MB-231 and BT-549 cells and the suppression by CAXII knockout. The relative CAXII expression in MCF-10 A was significantly lower than that of MDA-MB-231 and BT-549, respectively (P

    Article Snippet: Human triple-negative breast cancer cell MDA-MB-231, BT-549, and human breast normal epithelial cell MCF-10 A were purchased from American Type Culture Collection (ATCC, USA) and were characterized using STR (Short Tandem Repeat) analysis for identity verification of human cell lines in January, 2022.

    Techniques: Multiple Displacement Amplification, Knock-Out, Expressing

    Aerotaxis of breast cancer cells is dependent on EGFR activation. (A) Immunofluorescence imaging of EGFR in MCF10A cells in the absence of EGF (-EGF), upon activation by EGF (+ EGF), or EGF + cetuximab, a monoclonal EGFR neutralising antibody (+ EGF + Cetux), (B) Immunofluorescence imaging of EGFR in the indicated tumour cells in their culture medium supplemented with EGF. Scale bars, 25 μm and 10 μm for high magnification views inserted in the upper-right corner of each image (x2.5). Steady-state EGFR (-EGF and + EGF/+Cetux conditions) is located at the plasma membrane, whereas it appears as intracellular dots upon activation (+ EGF condition). (C) Aerotactic migration at 48 h of the indicated tumour cells either untreated (Mock), treated with 25 µg/mL or with 12.5 µg/mL cetuximab (Cetux). Scale bars 0.5 mm. (D) Cell distribution from the centre of the cell cluster (in mm) at 48 h in the Mock condition (carmine, mean of three independent experiments), in cells treated with 25 µg/mL (blue) or with 12.5 µg/mL (yellow). Y-axis scale is expressed in arbitrary unit. See Figure S 5 for the other tumour cells

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: EGFR-dependent aerotaxis is a common trait of breast tumour cells

    doi: 10.1186/s13046-022-02514-y

    Figure Lengend Snippet: Aerotaxis of breast cancer cells is dependent on EGFR activation. (A) Immunofluorescence imaging of EGFR in MCF10A cells in the absence of EGF (-EGF), upon activation by EGF (+ EGF), or EGF + cetuximab, a monoclonal EGFR neutralising antibody (+ EGF + Cetux), (B) Immunofluorescence imaging of EGFR in the indicated tumour cells in their culture medium supplemented with EGF. Scale bars, 25 μm and 10 μm for high magnification views inserted in the upper-right corner of each image (x2.5). Steady-state EGFR (-EGF and + EGF/+Cetux conditions) is located at the plasma membrane, whereas it appears as intracellular dots upon activation (+ EGF condition). (C) Aerotactic migration at 48 h of the indicated tumour cells either untreated (Mock), treated with 25 µg/mL or with 12.5 µg/mL cetuximab (Cetux). Scale bars 0.5 mm. (D) Cell distribution from the centre of the cell cluster (in mm) at 48 h in the Mock condition (carmine, mean of three independent experiments), in cells treated with 25 µg/mL (blue) or with 12.5 µg/mL (yellow). Y-axis scale is expressed in arbitrary unit. See Figure S 5 for the other tumour cells

    Article Snippet: The mammary epithelial cell line MCF10A, a spontaneous immortalised but non-transformed cell line, was obtained from the American Type Culture Collection.

    Techniques: Activation Assay, Immunofluorescence, Imaging, Migration

    Aerotactic migration of Hs578T and MDA-MB-231 is independent of EGFR activation. (A) Representative images taken at 48 h of MCF10A, Hs578T and MDA-MB-231 cell lines subjected to the aerotactic assay incubated in MCF10A or BC medium supplemented or not with cetuximab as indicated. Contrary to MCF10A, aerotactic migration of both HS578T and MDA-MB-231 is not affected by cetuximab. Scale bars, 0.5 mm. (B) Cell distribution from the centre of the cell cluster (in mm) at 0 h (green) and 48 h (carmine) in the -cetuximab condition and at 0 h (yellow) and 48 h (blue) in the + cetuximab condition is indicated as graphs (mean of three experiments). Y-axis scale is expressed in arbitrary unit. To assess the significant difference between the plus and minus cetuximab conditions at 48 h, a Student t-test was performed on D5% values. The p-values are indicated within graphs. NS non-significant

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: EGFR-dependent aerotaxis is a common trait of breast tumour cells

    doi: 10.1186/s13046-022-02514-y

    Figure Lengend Snippet: Aerotactic migration of Hs578T and MDA-MB-231 is independent of EGFR activation. (A) Representative images taken at 48 h of MCF10A, Hs578T and MDA-MB-231 cell lines subjected to the aerotactic assay incubated in MCF10A or BC medium supplemented or not with cetuximab as indicated. Contrary to MCF10A, aerotactic migration of both HS578T and MDA-MB-231 is not affected by cetuximab. Scale bars, 0.5 mm. (B) Cell distribution from the centre of the cell cluster (in mm) at 0 h (green) and 48 h (carmine) in the -cetuximab condition and at 0 h (yellow) and 48 h (blue) in the + cetuximab condition is indicated as graphs (mean of three experiments). Y-axis scale is expressed in arbitrary unit. To assess the significant difference between the plus and minus cetuximab conditions at 48 h, a Student t-test was performed on D5% values. The p-values are indicated within graphs. NS non-significant

    Article Snippet: The mammary epithelial cell line MCF10A, a spontaneous immortalised but non-transformed cell line, was obtained from the American Type Culture Collection.

    Techniques: Migration, Multiple Displacement Amplification, Activation Assay, Incubation

    Aerotaxis triggers invasion of tumour cells through extracellular matrix (Cultrex BME). (A) Aerotactic assay of untransformed MCF10A cells within the ECM (+ ECM) compared to the standard condition (-ECM). (B) Cell distribution from the centre of the cell cluster (in mm) of MCF10A at 0 h (carmine), 30 h (blue) or 60 h (yellow) in ECM and standard conditions. Y-axis scale is expressed in arbitrary unit. (C) Aerotactic assay of T6 tumour cells within the ECM compared to the standard condition (-ECM). Scale bars in A and C : 0.5 mm. (D) Cell distribution from the centre of the cell cluster (in mm) of T6 tumour cells at 0 h (carmine), 30 h (blue) or 60 h (yellow) in the ECM and standard conditions (mean of three independent experiments). Y-axis scale is expressed in arbitrary unit. (E) Magnified image of the aerotactic invasion of T6 tumour cells within the ECM at 18, 36 and 62 h after confinement. Scale bars, 250 μm

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: EGFR-dependent aerotaxis is a common trait of breast tumour cells

    doi: 10.1186/s13046-022-02514-y

    Figure Lengend Snippet: Aerotaxis triggers invasion of tumour cells through extracellular matrix (Cultrex BME). (A) Aerotactic assay of untransformed MCF10A cells within the ECM (+ ECM) compared to the standard condition (-ECM). (B) Cell distribution from the centre of the cell cluster (in mm) of MCF10A at 0 h (carmine), 30 h (blue) or 60 h (yellow) in ECM and standard conditions. Y-axis scale is expressed in arbitrary unit. (C) Aerotactic assay of T6 tumour cells within the ECM compared to the standard condition (-ECM). Scale bars in A and C : 0.5 mm. (D) Cell distribution from the centre of the cell cluster (in mm) of T6 tumour cells at 0 h (carmine), 30 h (blue) or 60 h (yellow) in the ECM and standard conditions (mean of three independent experiments). Y-axis scale is expressed in arbitrary unit. (E) Magnified image of the aerotactic invasion of T6 tumour cells within the ECM at 18, 36 and 62 h after confinement. Scale bars, 250 μm

    Article Snippet: The mammary epithelial cell line MCF10A, a spontaneous immortalised but non-transformed cell line, was obtained from the American Type Culture Collection.

    Techniques:

    Migration of breast tumour cells in the aerotactic assay. (A) Schematic representation of the aerotactic migration assay. (B) Radial aerotactic migration from the central cluster of untransformed MCF10A cells in a 96-well plate over 24 and 48 h. The graph on the right corresponds to the distribution of MCF10A cells from the centre of the well (in mm) at 0 h (yellow), 24 h (blue) and 48 h (carmine) after confinement (mean of three experiments). The Y-axis represents cell-density in arbitrary unit as described in the Methods section. (C) Classification of tumour cells relative to MCF10A according to their speed of migration in the aerotactic test. D5% is the minimum distance travelled by the fastest 5% cells (see Methods). (D) Representative images taken at 48 h corresponding to the aerotactic migration of T4, T5, T6, T7, T8, T11, T12, T13, T17, T18, T19 and T20 tumour cells subjected to the aerotactic assay. Cell distribution from the centre of the well at 0 h (yellow) and 48 h (carmine) is indicated as graphs (mean of three experiments). (See also Figure S 1 for T9, T10, T15 and T16 cells). Y-axis scale is expressed in arbitrary unit. Scale bars, 0.5 mm

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: EGFR-dependent aerotaxis is a common trait of breast tumour cells

    doi: 10.1186/s13046-022-02514-y

    Figure Lengend Snippet: Migration of breast tumour cells in the aerotactic assay. (A) Schematic representation of the aerotactic migration assay. (B) Radial aerotactic migration from the central cluster of untransformed MCF10A cells in a 96-well plate over 24 and 48 h. The graph on the right corresponds to the distribution of MCF10A cells from the centre of the well (in mm) at 0 h (yellow), 24 h (blue) and 48 h (carmine) after confinement (mean of three experiments). The Y-axis represents cell-density in arbitrary unit as described in the Methods section. (C) Classification of tumour cells relative to MCF10A according to their speed of migration in the aerotactic test. D5% is the minimum distance travelled by the fastest 5% cells (see Methods). (D) Representative images taken at 48 h corresponding to the aerotactic migration of T4, T5, T6, T7, T8, T11, T12, T13, T17, T18, T19 and T20 tumour cells subjected to the aerotactic assay. Cell distribution from the centre of the well at 0 h (yellow) and 48 h (carmine) is indicated as graphs (mean of three experiments). (See also Figure S 1 for T9, T10, T15 and T16 cells). Y-axis scale is expressed in arbitrary unit. Scale bars, 0.5 mm

    Article Snippet: The mammary epithelial cell line MCF10A, a spontaneous immortalised but non-transformed cell line, was obtained from the American Type Culture Collection.

    Techniques: Migration

    Comparison of aerotaxis versus intrinsic migration of breast cancer cells. 24 h trajectories of 50 representative cells per condition followed as XY plots (scales in µm) of the indicated cells either (A) plated at 5% confluency (intrinsic migration) or (B) subjected to the aerotactic assay (see also Figure S 2 for T5, T6, T8 T10 and T16 cells). (C) Mean values of speed ± SD (in µm/min) of the indicated cells (10 A stands for MCF10A). (D) Mean relative distance of migration ± SD (in µm) of the indicated cells at 24 h. (E) Mean directionality ± SD (no unit) is given for aerotaxis (blue) and intrinsic (carmine) cell migration. (F) Graphical representation of the intrinsic speed of tumour cells as a function of the relative distance travelled in the aerotactic test. Pearson’s correlation coefficient is given. (All of the data displayed are representative of at least 3 independent experiments)

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: EGFR-dependent aerotaxis is a common trait of breast tumour cells

    doi: 10.1186/s13046-022-02514-y

    Figure Lengend Snippet: Comparison of aerotaxis versus intrinsic migration of breast cancer cells. 24 h trajectories of 50 representative cells per condition followed as XY plots (scales in µm) of the indicated cells either (A) plated at 5% confluency (intrinsic migration) or (B) subjected to the aerotactic assay (see also Figure S 2 for T5, T6, T8 T10 and T16 cells). (C) Mean values of speed ± SD (in µm/min) of the indicated cells (10 A stands for MCF10A). (D) Mean relative distance of migration ± SD (in µm) of the indicated cells at 24 h. (E) Mean directionality ± SD (no unit) is given for aerotaxis (blue) and intrinsic (carmine) cell migration. (F) Graphical representation of the intrinsic speed of tumour cells as a function of the relative distance travelled in the aerotactic test. Pearson’s correlation coefficient is given. (All of the data displayed are representative of at least 3 independent experiments)

    Article Snippet: The mammary epithelial cell line MCF10A, a spontaneous immortalised but non-transformed cell line, was obtained from the American Type Culture Collection.

    Techniques: Migration

    Interferon γ-mediated autophagy contributes to the production of ADMA. 4T1 breast cancer cells were treated with low dose (0.2 ng/mL) or high dose (200 ng/mL) IFN-γ for 3 days. Induction of autophagy was measured using Premo LC3B-RFP viral transduction. The high dose of IFN-γ induced autophagy of 4T1 cells ( A ). The same dose of IFN-γ also led to increased secretion of ADMA into the conditioned medium in mouse breast cancer 4T1 cells ( B ) and IFN-γ induced ADMA secretion was blocked in 4T1 cells when 250 nM autophagy inhibitor was added to the culture ( B ). The effect of IFN-γ on the EMT 6 ( C ) or mouse normal mammary epithelial cell line, HC11, was not significant and unaffected by the autophagy inhibitor ( D ). ADMA concentration was expressed as ng/mL (left Y axis) or µM (right Y axis). Data are presented as mean ± SEM. (*p

    Journal: Cancer Cell International

    Article Title: Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization

    doi: 10.1186/s12935-022-02769-7

    Figure Lengend Snippet: Interferon γ-mediated autophagy contributes to the production of ADMA. 4T1 breast cancer cells were treated with low dose (0.2 ng/mL) or high dose (200 ng/mL) IFN-γ for 3 days. Induction of autophagy was measured using Premo LC3B-RFP viral transduction. The high dose of IFN-γ induced autophagy of 4T1 cells ( A ). The same dose of IFN-γ also led to increased secretion of ADMA into the conditioned medium in mouse breast cancer 4T1 cells ( B ) and IFN-γ induced ADMA secretion was blocked in 4T1 cells when 250 nM autophagy inhibitor was added to the culture ( B ). The effect of IFN-γ on the EMT 6 ( C ) or mouse normal mammary epithelial cell line, HC11, was not significant and unaffected by the autophagy inhibitor ( D ). ADMA concentration was expressed as ng/mL (left Y axis) or µM (right Y axis). Data are presented as mean ± SEM. (*p

    Article Snippet: Mouse breast cancer 4T1 cells (CRL-2539™, ATCC), EMT6 (CRL-2755™, ATCC), and normal mouse mammary HC11 cells (CRL-3062™, ATCC) were routinely cultured in RPMI 1640 (10-040-CV, Corning) with 10% FBS (10082-147, Gibco) and 1% penicillin plus streptomycin supplement (SV30010, Citiva) at 37 °C in a high humidity, 5% CO2 and 95% air incubator.

    Techniques: Transduction, Concentration Assay

    All Normal, Bulk Tumor Cells and CSCs Made ADMA but the Expression of PRMT1 and Jmjd6 was Different. All cell lines including mouse normal-like epithelial HC11, mouse breast cancer EMT6 and 4T1 cells were capable of generating and secreting ADMA into conditioned medium ( A ). The same was also observed using the stem cell cultures ( B ). RNA expression analyses showed that normal- like HC11 cells had high expression of the arginine methylating enzyme, PRMT1 ( C ) but also very high expression of the arginine demethylating enzyme, jmjd6 ( D ). The tumor cells, EMT6 and 4T1, by contrast, expressed lower levels of PRMT1 (C) and even lower relative levels of jmjd6 ( D ). ADMA concentration was expressed as ng/mL (left Y axis) or µM (right Y axis). Data are presented as individual replicates or mean ± SD. (*p

    Journal: Cancer Cell International

    Article Title: Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization

    doi: 10.1186/s12935-022-02769-7

    Figure Lengend Snippet: All Normal, Bulk Tumor Cells and CSCs Made ADMA but the Expression of PRMT1 and Jmjd6 was Different. All cell lines including mouse normal-like epithelial HC11, mouse breast cancer EMT6 and 4T1 cells were capable of generating and secreting ADMA into conditioned medium ( A ). The same was also observed using the stem cell cultures ( B ). RNA expression analyses showed that normal- like HC11 cells had high expression of the arginine methylating enzyme, PRMT1 ( C ) but also very high expression of the arginine demethylating enzyme, jmjd6 ( D ). The tumor cells, EMT6 and 4T1, by contrast, expressed lower levels of PRMT1 (C) and even lower relative levels of jmjd6 ( D ). ADMA concentration was expressed as ng/mL (left Y axis) or µM (right Y axis). Data are presented as individual replicates or mean ± SD. (*p

    Article Snippet: Mouse breast cancer 4T1 cells (CRL-2539™, ATCC), EMT6 (CRL-2755™, ATCC), and normal mouse mammary HC11 cells (CRL-3062™, ATCC) were routinely cultured in RPMI 1640 (10-040-CV, Corning) with 10% FBS (10082-147, Gibco) and 1% penicillin plus streptomycin supplement (SV30010, Citiva) at 37 °C in a high humidity, 5% CO2 and 95% air incubator.

    Techniques: Expressing, RNA Expression, Concentration Assay

    ADMA effect on Tumor Cell Number and Mesenchymal Markers. HC11, EMT6 or 4T1 cells were treated with DPBS (control) or ADMA (5 mM) for 3 days. Cell number was determined by MTS assay. There was a small effect of ADMA on cell growth no matter whether the tumor cells were cultured in regular RPMI 1640 ( A ) or the arginine- and lysine-free RPMI 1640 medium ( B ). Similarly, the same small impact of ADMA on stem cell proliferation was observed ( C ). EMT6 breast cancer cells were used to examine the impact of ADMA on the epithelial to mesenchymal transition (EMT). The mesenchymal related markers, vimentin and snail2, were increased and fibronectin was not affected by ADMA treatment ( D ). Epithelial mucin-1 was decreased by ADMA treatment ( E ). Data are presented as individual replicates with the mean ± SD. (*p

    Journal: Cancer Cell International

    Article Title: Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization

    doi: 10.1186/s12935-022-02769-7

    Figure Lengend Snippet: ADMA effect on Tumor Cell Number and Mesenchymal Markers. HC11, EMT6 or 4T1 cells were treated with DPBS (control) or ADMA (5 mM) for 3 days. Cell number was determined by MTS assay. There was a small effect of ADMA on cell growth no matter whether the tumor cells were cultured in regular RPMI 1640 ( A ) or the arginine- and lysine-free RPMI 1640 medium ( B ). Similarly, the same small impact of ADMA on stem cell proliferation was observed ( C ). EMT6 breast cancer cells were used to examine the impact of ADMA on the epithelial to mesenchymal transition (EMT). The mesenchymal related markers, vimentin and snail2, were increased and fibronectin was not affected by ADMA treatment ( D ). Epithelial mucin-1 was decreased by ADMA treatment ( E ). Data are presented as individual replicates with the mean ± SD. (*p

    Article Snippet: Mouse breast cancer 4T1 cells (CRL-2539™, ATCC), EMT6 (CRL-2755™, ATCC), and normal mouse mammary HC11 cells (CRL-3062™, ATCC) were routinely cultured in RPMI 1640 (10-040-CV, Corning) with 10% FBS (10082-147, Gibco) and 1% penicillin plus streptomycin supplement (SV30010, Citiva) at 37 °C in a high humidity, 5% CO2 and 95% air incubator.

    Techniques: MTS Assay, Cell Culture