Journal: Nucleic Acids Research

Article Title: Condensins-mediated mitotic folding persists as a structural echo in interphase chromatin

doi: 10.1093/nar/gkag1014

Figure Lengend Snippet: Orthogonal validation of compartment mixing by replicative labeling and microscopy. ( A ) Schematic of the BrdU/EdU pulse-chase labeling strategy. BrdU (1 h) labels early-replicating A-compartment DNA; after a 4 h chase, EdU (20 min) labels late-replicating B-compartment DNA. Cells are arrested in G1 with palbociclib before fixation. ( B ) Representative microscopy images showing DAPI, BrdU, EdU, and merged channels; scale bar: 5 µm. ( C ). Box plots of colocalization metrics (Pearson’s coefficient, overlap coefficient, Manders’ M1, and Manders’ M2) per cell across conditions. Exact P -values from Mann–Whitney U test are shown above brackets. Colocalization analysis: Ncaph–AID n = 546 (DMSO), 954 (Aux); Rad21–AID n = 1, 836 (DMSO), 1, 348 (Aux) cells from two independent experiments.

Article Snippet: For replicative labeling of compartments, cells were treated with 10 μM BrdU for 1 h and 10 μM EdU (both Lumiprobe, Russia) for 20 min, separated by a 4 h interval.

Techniques: Biomarker Discovery, Labeling, Microscopy, Pulse Chase, MANN-WHITNEY, Analysis

Journal: bioRxiv

Article Title: Diacylglycerol Kinases Balance DAG Signaling to Control B Cell Maturation, Germinal Center Homeostasis, and Humoral Immunity

doi: 10.64898/2026.10.05.756823

Figure Lengend Snippet: (A) Frequencies and absolute numbers of GL7 + Fas + GC B cells in the spleen and Peyer’s patches (PPs) of 8- to 10-week-old EGFP-DGKζΔNLS-AID Cre/+ and WT-AID Cre/+ mice. (B–E) Splenocytes and PP cells from EGFP-DGKζΔNLS-AID Cre/+ and EGFP-DGKζKD-AID Cre/+ mice were analyzed. (B) Representative flow cytometric plots showing GL7 and Fas expression among live B220 + IgM - IgD - B cells and GFP expression within GC B cells. (C) Frequencies and absolute numbers of GFP + cells within the GC B cell population. (D) Frequencies of BrdU + cells among GFP + GC B cells 16 h after intraperitoneal injection of BrdU. (E) Death rates of GFP + GC B cells. (F) Serum total and high-affinity NP-specific IgM and IgG titers in EGFP-DGKζΔNLS-AID Cre/+ and WT-AID Cre/+ mice at 7, 14, and 21 days after intraperitoneal immunization with NP-CGG in alum. Total and high-affinity NP-specific antibodies were measured using NP4-BSA and NP26-BSA, respectively. Each symbol represents one mouse. Data are representative of or pooled from at least three independent experiments and are presented as mean ± SEM where indicated. n.s., not significant; P < 0.05; P < 0.01; P < 0.001; P < 0.0001, as determined by unpaired two-tailed Student’s t-test.

Article Snippet: Mice were administered BrdU (1.5mg, MP Biomedicals) by intraperitoneal injection.

Techniques: Expressing, Injection, Two Tailed Test