brdu  (Millipore)

 
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    Name:
    2 Deoxyuridine
    Description:

    Catalog Number:
    d5412
    Price:
    None
    Applications:
    2'-Deoxyuridine (dU) is frequently halogenated to create thymidine analogues useful for studies of DNA synthesis and degradation mechanisms. Derivatized 2'-Deoxyuridines used as labeling substrates include chloro-2'-deoxyuridine (CldU), bromodeoxyuridine (BrdU) and/or iododeoxyuridine (IdU). Other useful analogues of 2'-deoxyuridine include 5-ethynyl-2'-deoxyuridine (DdU) and 5-hydroxymethyl-2'-deoxyuridine (HmdU).
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    Structured Review

    Millipore brdu
    2 Deoxyuridine

    https://www.bioz.com/result/brdu/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brdu - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Brain-Derived Neurotrophic Factor Participates in Determination of Neuronal Laminar Fate in the Developing Mouse Cerebral Cortex"

    Article Title: Brain-Derived Neurotrophic Factor Participates in Determination of Neuronal Laminar Fate in the Developing Mouse Cerebral Cortex

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4251-06.2006

    Progenitors expressing a lower level of Pax6 protein were increased in number after BDNF administration. BDNF (600 ng in 2 μl of PBS) was administered into the ventricular space of E13.5 mouse embryos 3 h after the injection of BrdU into pregnant
    Figure Legend Snippet: Progenitors expressing a lower level of Pax6 protein were increased in number after BDNF administration. BDNF (600 ng in 2 μl of PBS) was administered into the ventricular space of E13.5 mouse embryos 3 h after the injection of BrdU into pregnant

    Techniques Used: Expressing, Injection

    BDNF facilitates interkinetic migration of cortical progenitors. BDNF (600 ng in 2 μl of PBS) was administered into the ventricular space of E13.5 mouse embryos 3 h after the injection of BrdU into pregnant mice. A , BrdU immunostaining patterns
    Figure Legend Snippet: BDNF facilitates interkinetic migration of cortical progenitors. BDNF (600 ng in 2 μl of PBS) was administered into the ventricular space of E13.5 mouse embryos 3 h after the injection of BrdU into pregnant mice. A , BrdU immunostaining patterns

    Techniques Used: Migration, Injection, Mouse Assay, Immunostaining

    2) Product Images from "Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells"

    Article Title: Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-19216-1

    Damaged mitochondrial DNA activates ZBP1/TBK1/IRF3 signaling pathway. Interaction between BrDU-labelled mtDNA and TFAM ( A ) and BrDU-labelled mtDNA and ZBP1 ( B ) in control and GOx-treated cells at 1 h. ( C ) Interaction between ZBP1/TBK1, IRF3/TBK1, ZBP1/P-Tyr and ZBP1/P-Ser in control and GOx-treated cells at 1 h. ( D ) Interaction between IRF3/TBK1 in pre-treated with 3 μM of CsA in GOx-treated BEAS 2B cells. ( E ) Level of ZBP1 depletion, shown by Western blotting. ( F ) Expression of IL-6 and IL-8 in unstressed and GOx-treated (0.006 U/ml for 1 h) in control and ZBP1-depleted BEAS2B cells. Representative images of n = 3 independent experiments are shown. Data represent average ± SEM of n = 5 biological replicates. *p
    Figure Legend Snippet: Damaged mitochondrial DNA activates ZBP1/TBK1/IRF3 signaling pathway. Interaction between BrDU-labelled mtDNA and TFAM ( A ) and BrDU-labelled mtDNA and ZBP1 ( B ) in control and GOx-treated cells at 1 h. ( C ) Interaction between ZBP1/TBK1, IRF3/TBK1, ZBP1/P-Tyr and ZBP1/P-Ser in control and GOx-treated cells at 1 h. ( D ) Interaction between IRF3/TBK1 in pre-treated with 3 μM of CsA in GOx-treated BEAS 2B cells. ( E ) Level of ZBP1 depletion, shown by Western blotting. ( F ) Expression of IL-6 and IL-8 in unstressed and GOx-treated (0.006 U/ml for 1 h) in control and ZBP1-depleted BEAS2B cells. Representative images of n = 3 independent experiments are shown. Data represent average ± SEM of n = 5 biological replicates. *p

    Techniques Used: Western Blot, Expressing

    3) Product Images from "Voluntary running rescues the defective hippocampal neurogenesis and behaviour observed in lipocalin 2-null mice"

    Article Title: Voluntary running rescues the defective hippocampal neurogenesis and behaviour observed in lipocalin 2-null mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-38140-y

    Voluntary running promotes hippocampal cell proliferation and survival, potentiating the transition of quiescent to proliferating NSCs in LCN2-null mice. ( a ) Schematic diagram of the experimental paradigm of voluntary running and of the BrdU injection protocol used. For 28 days, Wt and LCN2-null mice were assigned as running, i.e. animals housed to have free access to a running wheel, and as sedentary, housed under standard housing conditions with no running wheel available. ( b ) Representative illustration of the hippocampal neurogenic process, including cellular types and the specific markers used. ( c ) Running increased cell proliferation (Ki67 + cells) in LCN2-null mice, and cell survival (BrdU + cells) in both Wt and LCN2-null mice (n = 4–6 per group). ( d ) Representative confocal images of Ki67 and BrdU immunostaining (indicated by white arrows) in the SGZ of the DG of Wt and LCN2-null sedentary and running mice. ( e ) Quantitative analysis of radial quiescent type-1 (GFAP + /BrdU + ) and amplifying type-2 stem cells (Sox2 + /Ki67 + ) after 28 days of running revealed a significant decrease in type-1 population in LCN2-null mice, and a consequent increase in type-2 cells (n = 5 mice per group). ( f ) Representative confocal images of GFAP + /BrdU + and Sox2 + /Ki67 + immunostaining in the SGZ of sedentary and running mice of both genotypes (indicated by white arrows). Scale bars, 50 μm. Data from the sedentary animals is the same as described in Ferreira et al , and are presented as mean ± SEM and were analyzed by two-way ANOVA with Bonferroni’s multiple comparison test. *Denotes differences between sedentary Wt and LCN2-null mice; δ between sedentary and running Wt; # between sedentary and running LCN2-null mice. # p ≤ 0.05, δδ,## p ≤ 0.01, **** ,#### p ≤ 0.0001.
    Figure Legend Snippet: Voluntary running promotes hippocampal cell proliferation and survival, potentiating the transition of quiescent to proliferating NSCs in LCN2-null mice. ( a ) Schematic diagram of the experimental paradigm of voluntary running and of the BrdU injection protocol used. For 28 days, Wt and LCN2-null mice were assigned as running, i.e. animals housed to have free access to a running wheel, and as sedentary, housed under standard housing conditions with no running wheel available. ( b ) Representative illustration of the hippocampal neurogenic process, including cellular types and the specific markers used. ( c ) Running increased cell proliferation (Ki67 + cells) in LCN2-null mice, and cell survival (BrdU + cells) in both Wt and LCN2-null mice (n = 4–6 per group). ( d ) Representative confocal images of Ki67 and BrdU immunostaining (indicated by white arrows) in the SGZ of the DG of Wt and LCN2-null sedentary and running mice. ( e ) Quantitative analysis of radial quiescent type-1 (GFAP + /BrdU + ) and amplifying type-2 stem cells (Sox2 + /Ki67 + ) after 28 days of running revealed a significant decrease in type-1 population in LCN2-null mice, and a consequent increase in type-2 cells (n = 5 mice per group). ( f ) Representative confocal images of GFAP + /BrdU + and Sox2 + /Ki67 + immunostaining in the SGZ of sedentary and running mice of both genotypes (indicated by white arrows). Scale bars, 50 μm. Data from the sedentary animals is the same as described in Ferreira et al , and are presented as mean ± SEM and were analyzed by two-way ANOVA with Bonferroni’s multiple comparison test. *Denotes differences between sedentary Wt and LCN2-null mice; δ between sedentary and running Wt; # between sedentary and running LCN2-null mice. # p ≤ 0.05, δδ,## p ≤ 0.01, **** ,#### p ≤ 0.0001.

    Techniques Used: Mouse Assay, Injection, Immunostaining

    4) Product Images from "Early Pharmacotherapy Restores Neurogenesis and Cognitive Performance in the Ts65Dn Mouse Model for Down Syndrome"

    Article Title: Early Pharmacotherapy Restores Neurogenesis and Cognitive Performance in the Ts65Dn Mouse Model for Down Syndrome

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0534-10.2010

    Effect of fluoxetine on cell proliferation in the dentate gyrus and subventricular zone of euploid and Ts65Dn mice. A , B , Examples of sections immunostained for BrdU and counterstained with hematoxylin from the DG ( A ) and SVZ ( B ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. These animals received one injection of BrdU on P15 and were killed after 2 h. Scale bars = 150 μm. C , D , Number of BrdU+ cells in the DG ( C ) and SVZ ( D ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. E , F , Number of caspase+ cells in the DG ( E ) and SVZ ( F ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. Values in C – F represent totals for one hemisphere (mean ± SD). * p
    Figure Legend Snippet: Effect of fluoxetine on cell proliferation in the dentate gyrus and subventricular zone of euploid and Ts65Dn mice. A , B , Examples of sections immunostained for BrdU and counterstained with hematoxylin from the DG ( A ) and SVZ ( B ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. These animals received one injection of BrdU on P15 and were killed after 2 h. Scale bars = 150 μm. C , D , Number of BrdU+ cells in the DG ( C ) and SVZ ( D ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. E , F , Number of caspase+ cells in the DG ( E ) and SVZ ( F ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. Values in C – F represent totals for one hemisphere (mean ± SD). * p

    Techniques Used: Mouse Assay, Injection

    Effect of fluoxetine on cell survival in the dentate gyrus of euploid and Ts65Dn mice. A , Examples of sections processed for fluorescent immunostaining for BrdU from the DG of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. These animals received one injection of BrdU on P15 and were killed after 1 month. Scale bars = 200 μm (lower magnification) and 40 μm (higher magnification). B – D , Number of BrdU+ cells in the whole DG (GR+HILUS) ( B ), in the granule cell layer ( C ), and in the hilus ( D ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. E , Number of caspase+ cells in the whole DG (GR+HILUS) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. Values in B – E represent totals for one DG (mean ± SD). * p
    Figure Legend Snippet: Effect of fluoxetine on cell survival in the dentate gyrus of euploid and Ts65Dn mice. A , Examples of sections processed for fluorescent immunostaining for BrdU from the DG of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. These animals received one injection of BrdU on P15 and were killed after 1 month. Scale bars = 200 μm (lower magnification) and 40 μm (higher magnification). B – D , Number of BrdU+ cells in the whole DG (GR+HILUS) ( B ), in the granule cell layer ( C ), and in the hilus ( D ) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. E , Number of caspase+ cells in the whole DG (GR+HILUS) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine. Values in B – E represent totals for one DG (mean ± SD). * p

    Techniques Used: Mouse Assay, Immunostaining, Injection

    Phenotype of the surviving cells in the dentate gyrus of euploid and Ts65Dn mice. A – F , Absolute number ( A – C ) and percentage ( D – F ) of surviving cells with neuronal phenotype (NeuN/BrdU), astrocytic phenotype (GFAP/BrdU), and undetermined phenotype (Neither/BrdU) in the DG (granule cell layer + hilus) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine (Fluo). These animals received one BrdU injection on P15 and were killed after 1 month (on P45). Values in ( A – C ) represent totals for one DG (mean ± SD). * p
    Figure Legend Snippet: Phenotype of the surviving cells in the dentate gyrus of euploid and Ts65Dn mice. A – F , Absolute number ( A – C ) and percentage ( D – F ) of surviving cells with neuronal phenotype (NeuN/BrdU), astrocytic phenotype (GFAP/BrdU), and undetermined phenotype (Neither/BrdU) in the DG (granule cell layer + hilus) of untreated euploid and Ts65Dn mice and euploid and Ts65Dn mice treated with fluoxetine (Fluo). These animals received one BrdU injection on P15 and were killed after 1 month (on P45). Values in ( A – C ) represent totals for one DG (mean ± SD). * p

    Techniques Used: Mouse Assay, Injection

    5) Product Images from "Neuronal Birthdate-Specific Gene Transfer with Adenoviral Vectors"

    Article Title: Neuronal Birthdate-Specific Gene Transfer with Adenoviral Vectors

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2529-03.2004

    BrdU labeling of an E11.5:E18.5 brain. A mixture of AdexCAG-NL-LacZ and BrdU was injected into the midbrain ventricle of an embryo on E11.5. At E18.5, the E11.5:E18.5 brain was sectioned transversely and double-immunostained with anti-β-gal (shown
    Figure Legend Snippet: BrdU labeling of an E11.5:E18.5 brain. A mixture of AdexCAG-NL-LacZ and BrdU was injected into the midbrain ventricle of an embryo on E11.5. At E18.5, the E11.5:E18.5 brain was sectioned transversely and double-immunostained with anti-β-gal (shown

    Techniques Used: Labeling, Injection

    Feature of adenovirus-mediated gene transfer into embryonic brain. AdexCAG-NL-LacZ and BrdU were simultaneously injected into the midbrain ventricle of E12.5 mouse embryos ( A-C, G ′). In the other cases, AdexCAG-NL-LacZ and BrdU were injected at
    Figure Legend Snippet: Feature of adenovirus-mediated gene transfer into embryonic brain. AdexCAG-NL-LacZ and BrdU were simultaneously injected into the midbrain ventricle of E12.5 mouse embryos ( A-C, G ′). In the other cases, AdexCAG-NL-LacZ and BrdU were injected at

    Techniques Used: Injection

    Related Articles

    Blocking Assay:

    Article Title: miR-125/CDK2 axis in cochlear progenitor cell proliferation
    Article Snippet: Subsequently, cells were incubated with 1% Triton X-100 on ice for 5 min, washed with PBS three times and blocked with 5% bovine serum albumin (cat. no. ST025; Beyotime Institute of Biotechnology) diluted in PBS. .. The blocking buffer was discarded and cells were incubated with primary antibodies against nestin (1:100; cat. no. MAB353; EMD Millipore), BrdU (1:500; cat. no. MAB4072; EMD Millipore) and myosin VIIA (1:100; cat. no. Ab150386; Abcam) at 4°C overnight. .. Subsequently, cells were washed with PBS three times and incubated with Cy3-labelled secondary antibody IgG (1:100; Sigma-Aldrich; Merck KGaA) at 37°C for 30 min.

    Incubation:

    Article Title: miR-125/CDK2 axis in cochlear progenitor cell proliferation
    Article Snippet: Subsequently, cells were incubated with 1% Triton X-100 on ice for 5 min, washed with PBS three times and blocked with 5% bovine serum albumin (cat. no. ST025; Beyotime Institute of Biotechnology) diluted in PBS. .. The blocking buffer was discarded and cells were incubated with primary antibodies against nestin (1:100; cat. no. MAB353; EMD Millipore), BrdU (1:500; cat. no. MAB4072; EMD Millipore) and myosin VIIA (1:100; cat. no. Ab150386; Abcam) at 4°C overnight. .. Subsequently, cells were washed with PBS three times and incubated with Cy3-labelled secondary antibody IgG (1:100; Sigma-Aldrich; Merck KGaA) at 37°C for 30 min.

    Article Title: TopBP1 functions with 53BP1 in the G1 DNA damage checkpoint
    Article Snippet: At 48 h after siRNA transfection, U2OS cells pulse-labelled for 1 h with 10 μM EdU (Invitrogen) were exposed to IR (2 Gy) or were mock-irradiated. .. Then, the cells were incubated for 7 h with 10 μM BrdU (Sigma) and 0.25 μg/ml nocodazole (Sigma). ..

    Article Title: Characterization of the p53-Dependent Postmitotic Checkpoint following Spindle Disruption
    Article Snippet: .. MEFs were allowed to adhere to coverslips for 24 h and were then changed into medium with or without nocodazole (0.125 μg/ml) and incubated for 24 h. Bromodeoxyuridine (BrdU) and fluorodeoxyuridine (FdU) (Sigma) were added to the medium from a 1,000× stock solution in H2 O, for a final concentration of 3 μg of BrdU plus 0.3 μg of FdU/ml of medium; cells were incubated for an additional 4 h. Coverslips were then fixed in 4% paraformaldehyde-PBS for 20 min at room temperature, washed in PBS, permeabilized for 15 min in 0.25% Triton X-100–PBS, and washed again with PBS. ..

    other:

    Article Title: Characterization of Nucleosides and Nucleobases in Natural Cordyceps by HILIC–ESI/TOF/MS and HILIC–ESI/MS
    Article Snippet: The standards of thymine, uracil, thymidine, 2'-deoxyuridine, cordycepin, uridine, hypoxanthine, adenine, adenosine, xanthine, inosine, cytosine, guanine, cytidine, guanosine and 2-chloroadenosine were purchased from Sigma-Aldrich (St. Louis, MO, USA). shows the chemical structures of these reference compounds.

    Cell Culture:

    Article Title: The Endogenous GRP78 Interactome in Human Head and Neck Cancers: A Deterministic Role of Cell Surface GRP78 in Cancer Stemness
    Article Snippet: The asymmetric cell division assay was modified from previously described protocols . .. Briefly, BM2 and OECM1 cells were cultured in their growth media containing 1 μM BrdU (Sigma-Aldrich) for 14 days, and FaDu cells 11 days. ..

    Labeling:

    Article Title: Brain Region-Dependent Rejection of Neural Precursor Cell Transplants
    Article Snippet: Quantities of 5 × 103 NPC spheres were injected into the naïve striatum ( A = 0.5 mm, L = 2 mm, H = 4.5 mm). .. Wild-type and CD200 KO NPC spheres were labeled with 30 μM BrdU (sigma) for 48 h prior to transplantation. .. In some experiments, mice were treated with daily injection of i.p. cyclosporine A (10 mg/kg) or saline as vehicle.

    Transplantation Assay:

    Article Title: Brain Region-Dependent Rejection of Neural Precursor Cell Transplants
    Article Snippet: Quantities of 5 × 103 NPC spheres were injected into the naïve striatum ( A = 0.5 mm, L = 2 mm, H = 4.5 mm). .. Wild-type and CD200 KO NPC spheres were labeled with 30 μM BrdU (sigma) for 48 h prior to transplantation. .. In some experiments, mice were treated with daily injection of i.p. cyclosporine A (10 mg/kg) or saline as vehicle.

    Concentration Assay:

    Article Title: Characterization of the p53-Dependent Postmitotic Checkpoint following Spindle Disruption
    Article Snippet: .. MEFs were allowed to adhere to coverslips for 24 h and were then changed into medium with or without nocodazole (0.125 μg/ml) and incubated for 24 h. Bromodeoxyuridine (BrdU) and fluorodeoxyuridine (FdU) (Sigma) were added to the medium from a 1,000× stock solution in H2 O, for a final concentration of 3 μg of BrdU plus 0.3 μg of FdU/ml of medium; cells were incubated for an additional 4 h. Coverslips were then fixed in 4% paraformaldehyde-PBS for 20 min at room temperature, washed in PBS, permeabilized for 15 min in 0.25% Triton X-100–PBS, and washed again with PBS. ..

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  • 93
    Millipore mouse anti brdu monoclonal antibody
    Reduced exdpf Causes Defects of Exocrine Cell Proliferation (A) <t>BrdU</t> labeling result in 33 hpf embryos. Green: MP760 <t>:GFP</t> , Red: BrdU staining. Blue: DAPI staining. Dorsal view, anterior to the left. Enlargement represents higher magnification of boxed area in each row. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors. (B) BrdU labeling result in 3 dpf embryos. Green: elastase A:GFP , Red: BrdU staining. Lateral view, anterior to the left. Scale bar: 50 μm. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors. (C) Quantitative graphs for BrdU incorporation rate in 33 hpf or 3 dpf embryos. The average number of GFP positive cells with BrdU incorporation was obtained by counting BrdU-labeled GFP positive cells from five embryos. Y axis: Mean ± SD. (D) Semiquantitative RT-PCR examination of expression of cyclin D1 and cell cycle inhibitors p21 , p27 and cyclin G1 in control (control), exdpf morphants (MO), and exdpf mRNA injected embryos (RNA) at 33 hpf, 2 dpf, 3 dpf, and 5 dpf. In each group, 30 embryos were used to extract total RNA for RT-PCR.
    Mouse Anti Brdu Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti brdu monoclonal antibody/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti brdu monoclonal antibody - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    93
    Millipore mouse anti brdu
    1N3R-tau inhibits cell proliferation measured by <t>BrdU</t> incorporation. Fluorescent micrographs of eGFP and BrdU expression with six tau isoforms in N2a cells after 48 h transfection. <t>GFP</t> (Green), BrdU (Red). Quantification of BrdU-positive cells in eGFP expressing N2a cells with six tau isoforms after 48 h transfection. Scale bar = 50 μm. All values are standardized with vector. * P
    Mouse Anti Brdu, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti brdu/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti brdu - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    98
    Millipore brdu
    Sertraline increases neurogenesis in subgranular zone (SGZ) of dentate gyrus in HD mice and control mice. (a) Sertraline (10 mg/kg) was administered to mice at 12 weeks of age for 4 weeks. Mice then received <t>5-bromo-2-deoxyuridine</t> <t>(BrdU)</t> and were perfused
    Brdu, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brdu/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brdu - by Bioz Stars, 2021-03
    98/100 stars
      Buy from Supplier

    Image Search Results


    Reduced exdpf Causes Defects of Exocrine Cell Proliferation (A) BrdU labeling result in 33 hpf embryos. Green: MP760 :GFP , Red: BrdU staining. Blue: DAPI staining. Dorsal view, anterior to the left. Enlargement represents higher magnification of boxed area in each row. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors. (B) BrdU labeling result in 3 dpf embryos. Green: elastase A:GFP , Red: BrdU staining. Lateral view, anterior to the left. Scale bar: 50 μm. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors. (C) Quantitative graphs for BrdU incorporation rate in 33 hpf or 3 dpf embryos. The average number of GFP positive cells with BrdU incorporation was obtained by counting BrdU-labeled GFP positive cells from five embryos. Y axis: Mean ± SD. (D) Semiquantitative RT-PCR examination of expression of cyclin D1 and cell cycle inhibitors p21 , p27 and cyclin G1 in control (control), exdpf morphants (MO), and exdpf mRNA injected embryos (RNA) at 33 hpf, 2 dpf, 3 dpf, and 5 dpf. In each group, 30 embryos were used to extract total RNA for RT-PCR.

    Journal: PLoS Biology

    Article Title: Exdpf Is a Key Regulator of Exocrine Pancreas Development Controlled by Retinoic Acid and ptf1a in Zebrafish

    doi: 10.1371/journal.pbio.0060293

    Figure Lengend Snippet: Reduced exdpf Causes Defects of Exocrine Cell Proliferation (A) BrdU labeling result in 33 hpf embryos. Green: MP760 :GFP , Red: BrdU staining. Blue: DAPI staining. Dorsal view, anterior to the left. Enlargement represents higher magnification of boxed area in each row. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors. (B) BrdU labeling result in 3 dpf embryos. Green: elastase A:GFP , Red: BrdU staining. Lateral view, anterior to the left. Scale bar: 50 μm. Arrows indicate non-proliferating cells; arrowheads indicate proliferating cells as evidenced by overlapping of red and green colors. (C) Quantitative graphs for BrdU incorporation rate in 33 hpf or 3 dpf embryos. The average number of GFP positive cells with BrdU incorporation was obtained by counting BrdU-labeled GFP positive cells from five embryos. Y axis: Mean ± SD. (D) Semiquantitative RT-PCR examination of expression of cyclin D1 and cell cycle inhibitors p21 , p27 and cyclin G1 in control (control), exdpf morphants (MO), and exdpf mRNA injected embryos (RNA) at 33 hpf, 2 dpf, 3 dpf, and 5 dpf. In each group, 30 embryos were used to extract total RNA for RT-PCR.

    Article Snippet: After washing (5 × 5 min in PBST), embryos were incubated in 1 N HCl for 1 h. Then embryos were washed again for 5 × 5 min in PBST and blocked using 5% goat serum for 1 h at room temperature and incubated over night at 4 °C in 1:100 mouse anti-BrdU monoclonal antibody (Chemicon) and 1:500 rabbit anti-GFP polyclonal antibody (Abcam).

    Techniques: Labeling, BrdU Staining, Staining, BrdU Incorporation Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Injection

    1N3R-tau inhibits cell proliferation measured by BrdU incorporation. Fluorescent micrographs of eGFP and BrdU expression with six tau isoforms in N2a cells after 48 h transfection. GFP (Green), BrdU (Red). Quantification of BrdU-positive cells in eGFP expressing N2a cells with six tau isoforms after 48 h transfection. Scale bar = 50 μm. All values are standardized with vector. * P

    Journal: PLoS ONE

    Article Title: Expression of 1N3R-Tau Isoform Inhibits Cell Proliferation by Inducing S Phase Arrest in N2a Cells

    doi: 10.1371/journal.pone.0119865

    Figure Lengend Snippet: 1N3R-tau inhibits cell proliferation measured by BrdU incorporation. Fluorescent micrographs of eGFP and BrdU expression with six tau isoforms in N2a cells after 48 h transfection. GFP (Green), BrdU (Red). Quantification of BrdU-positive cells in eGFP expressing N2a cells with six tau isoforms after 48 h transfection. Scale bar = 50 μm. All values are standardized with vector. * P

    Article Snippet: Cells were respectively incubated with mouse anti-BrdU (1:200, Millipore, MAB3222) and rabbit anti-GFP (1:1000, Abcam, ab290) overnight at 4°C one after another.

    Techniques: BrdU Incorporation Assay, Expressing, Transfection, Plasmid Preparation

    Sertraline increases neurogenesis in subgranular zone (SGZ) of dentate gyrus in HD mice and control mice. (a) Sertraline (10 mg/kg) was administered to mice at 12 weeks of age for 4 weeks. Mice then received 5-bromo-2-deoxyuridine (BrdU) and were perfused

    Journal:

    Article Title: Sertraline Slows Disease Progression and Increases Neurogenesis in N171-82Q mouse model of Huntington's Disease

    doi: 10.1016/j.nbd.2008.01.015

    Figure Lengend Snippet: Sertraline increases neurogenesis in subgranular zone (SGZ) of dentate gyrus in HD mice and control mice. (a) Sertraline (10 mg/kg) was administered to mice at 12 weeks of age for 4 weeks. Mice then received 5-bromo-2-deoxyuridine (BrdU) and were perfused

    Article Snippet: For the neurogenesis study, all mice were received twice daily injections of 50 mg/kg BrdU (5-bromo-2-deoxyuridine; Sigma) in sterile 0.9% NaCl solution at 8 h apart for 5 consecutive days.

    Techniques: Mouse Assay

    MEAF6-1 promotes prostate cancer cell growth and invasion Using LNCaP or PC-3 cell lines stably expressing control (CTL), MEAF6-1, or MEAF6-2, ( A ) 2D BrdU cell proliferation assays were performed to measure proliferation of cells. BrdU results represent colorimetric quantitative measurements (optical density, OD, at 450 nm wavelength) of cellular BrdU incorporation into DNA. ( B ) Cells were also seeded in matrigel for BrdU proliferation assays to measure the proliferative ability under 3D conditions. ( C ) Soft agar assays were performed on LNCaP cells in a 6-well plate. Colonies were stained with crystal violet and counted by varied sizes (i.e. 100–300 um or > 300 um). Images of wells were captured by Zeiss light microscope, representing one of the three independent biological replicates. ( D ) PC-3 stable cells were seeded in 6-well plates and incubated for 16 hours for wound healing assays. Area of migration measured using Zen imaging software. Cell invasion ability of PC-3 cell lines was measured using matrigel invasion chambers. All results are presented as the mean ± SEM (one-way ANOVA; n = 3; ***denotes p

    Journal: Oncotarget

    Article Title: Alternative RNA splicing of the MEAF6 gene facilitates neuroendocrine prostate cancer progression

    doi: 10.18632/oncotarget.15854

    Figure Lengend Snippet: MEAF6-1 promotes prostate cancer cell growth and invasion Using LNCaP or PC-3 cell lines stably expressing control (CTL), MEAF6-1, or MEAF6-2, ( A ) 2D BrdU cell proliferation assays were performed to measure proliferation of cells. BrdU results represent colorimetric quantitative measurements (optical density, OD, at 450 nm wavelength) of cellular BrdU incorporation into DNA. ( B ) Cells were also seeded in matrigel for BrdU proliferation assays to measure the proliferative ability under 3D conditions. ( C ) Soft agar assays were performed on LNCaP cells in a 6-well plate. Colonies were stained with crystal violet and counted by varied sizes (i.e. 100–300 um or > 300 um). Images of wells were captured by Zeiss light microscope, representing one of the three independent biological replicates. ( D ) PC-3 stable cells were seeded in 6-well plates and incubated for 16 hours for wound healing assays. Area of migration measured using Zen imaging software. Cell invasion ability of PC-3 cell lines was measured using matrigel invasion chambers. All results are presented as the mean ± SEM (one-way ANOVA; n = 3; ***denotes p

    Article Snippet: Briefly, 2D and 3D cell proliferation was measured with the bromodeoxyuridine (BrdU) proliferation assay kit (Millipore, Catalogue # 2750) according to manufacturer's protocol and as previously described [ ].

    Techniques: Stable Transfection, Expressing, CTL Assay, BrdU Incorporation Assay, Staining, Light Microscopy, Incubation, Migration, Imaging, Software