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Becton Dickinson brdu
The genome‐wide association of sororin with cohesin occurs exclusively on replicated DNA Examples of sororin (dotted arrows) and <t>SMC3</t> ChIP‐seq and <t>BrdU</t> DIP‐seq data from early S‐phase as compared to G2‐phase using the Integrated Genome Browser (IGB; Nicol et al , 2009 ). Alignment of sororin, BrdU, and BrdU‐negative control sequencing bins in early S‐phase to human chromosome 18 (GRCh37/hg19). Bars correspond to called peaks, curves to read enrichments after smoothing ( k = 25 kbp). Quantification of the co‐occupancy of BrdU incorporation and sororin localization in early S‐phase and sororin in G2‐phase depicted as P ‐value distributions calculated with IntervalStats (Chikina Troyanskaya, 2012 ) and compared to randomized controls. Sororin peaks were identified as overlapping peaks of 4 samples in early S‐phase and 2 samples in G2‐phase, using only the common peaks. Quantification of the co‐occupancy of SMC3 in early S‐phase and in G2‐phase with BrdU incorporation in early S‐phase.
Brdu, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Sororin actively maintains sister chromatid cohesion"

Article Title: Sororin actively maintains sister chromatid cohesion

Journal: The EMBO Journal

doi: 10.15252/embj.201592532

The genome‐wide association of sororin with cohesin occurs exclusively on replicated DNA Examples of sororin (dotted arrows) and SMC3 ChIP‐seq and BrdU DIP‐seq data from early S‐phase as compared to G2‐phase using the Integrated Genome Browser (IGB; Nicol et al , 2009 ). Alignment of sororin, BrdU, and BrdU‐negative control sequencing bins in early S‐phase to human chromosome 18 (GRCh37/hg19). Bars correspond to called peaks, curves to read enrichments after smoothing ( k = 25 kbp). Quantification of the co‐occupancy of BrdU incorporation and sororin localization in early S‐phase and sororin in G2‐phase depicted as P ‐value distributions calculated with IntervalStats (Chikina Troyanskaya, 2012 ) and compared to randomized controls. Sororin peaks were identified as overlapping peaks of 4 samples in early S‐phase and 2 samples in G2‐phase, using only the common peaks. Quantification of the co‐occupancy of SMC3 in early S‐phase and in G2‐phase with BrdU incorporation in early S‐phase.
Figure Legend Snippet: The genome‐wide association of sororin with cohesin occurs exclusively on replicated DNA Examples of sororin (dotted arrows) and SMC3 ChIP‐seq and BrdU DIP‐seq data from early S‐phase as compared to G2‐phase using the Integrated Genome Browser (IGB; Nicol et al , 2009 ). Alignment of sororin, BrdU, and BrdU‐negative control sequencing bins in early S‐phase to human chromosome 18 (GRCh37/hg19). Bars correspond to called peaks, curves to read enrichments after smoothing ( k = 25 kbp). Quantification of the co‐occupancy of BrdU incorporation and sororin localization in early S‐phase and sororin in G2‐phase depicted as P ‐value distributions calculated with IntervalStats (Chikina Troyanskaya, 2012 ) and compared to randomized controls. Sororin peaks were identified as overlapping peaks of 4 samples in early S‐phase and 2 samples in G2‐phase, using only the common peaks. Quantification of the co‐occupancy of SMC3 in early S‐phase and in G2‐phase with BrdU incorporation in early S‐phase.

Techniques Used: GWAS, Chromatin Immunoprecipitation, DNA Immunoprecipitation Sequencing, Negative Control, Sequencing, BrdU Incorporation Assay

The genome‐wide association of sororin with cohesin occurs exclusively on replicated DNA (related to Fig 4 ) Experimental setup used to synchronize HeLa cells in different states of DNA replication after double thymidine arrest–release (DTAR). Examples of BrdU sequencing enrichment in early, mid‐, and late S‐phase. Please note the differences in y ‐axis scale between different time points. The varying sequencing enrichment possibly represents differences in population‐wide replication dynamics. Quantification of the co‐occupancy of BrdU incorporation and sororin localization in early, mid‐, and late S‐phase depicted as P ‐value distributions and compared to randomized controls. Please note that in late S‐phase, the co‐occupancy between sororin and BrdU is low, presumably because a large part of the genome was replicated and bound by sororin before the BrdU pulse. Experimental setup to synchronize HeLa cells at two time points corresponding to early S‐phase. Graph depicting the co‐occupancy of BrdU and sororin at S‐phase time points t1 and t2. Examples of sororin and SMC3 ChIP‐seq and BrdU DIP‐seq data from early S‐phase as compared to G2‐phase.
Figure Legend Snippet: The genome‐wide association of sororin with cohesin occurs exclusively on replicated DNA (related to Fig 4 ) Experimental setup used to synchronize HeLa cells in different states of DNA replication after double thymidine arrest–release (DTAR). Examples of BrdU sequencing enrichment in early, mid‐, and late S‐phase. Please note the differences in y ‐axis scale between different time points. The varying sequencing enrichment possibly represents differences in population‐wide replication dynamics. Quantification of the co‐occupancy of BrdU incorporation and sororin localization in early, mid‐, and late S‐phase depicted as P ‐value distributions and compared to randomized controls. Please note that in late S‐phase, the co‐occupancy between sororin and BrdU is low, presumably because a large part of the genome was replicated and bound by sororin before the BrdU pulse. Experimental setup to synchronize HeLa cells at two time points corresponding to early S‐phase. Graph depicting the co‐occupancy of BrdU and sororin at S‐phase time points t1 and t2. Examples of sororin and SMC3 ChIP‐seq and BrdU DIP‐seq data from early S‐phase as compared to G2‐phase.

Techniques Used: GWAS, Sequencing, BrdU Incorporation Assay, Chromatin Immunoprecipitation, DNA Immunoprecipitation Sequencing

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Incubation:

Article Title: Human NK cell deficiency as a result of biallelic mutations in MCM10
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Staining:

Article Title: Human NK cell deficiency as a result of biallelic mutations in MCM10
Article Snippet: These conditions were established to not allow cells to become greater than 90% confluent during this assay to prevent contact inhibition from affecting doubling time analysis. .. Cell cycle analysesFor analysis of cell cycle, NK92 cells or primary patient fibroblasts were incubated with BrdU for 12 hours prior to fixation and staining with 7-AAD (BD Biosciences). .. Cells were analyzed via FACS on a BD Fortessa with 18-color configuration and resulting data were evaluated using Kaluza (Beckman-Coulter) or FlowJo (TreeStar) software.

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Article Title: A UVB-responsive common variant at chr7p21.1 confers tanning response and melanoma risk via regulation of the aryl hydrocarbon receptor gene (AHR)
Article Snippet: Briefly, human melanocytes were labeled with 10 μM BrdU for 3 hours before they were fixed, permeabilized, and subjected to DNase I treatment. .. Cells were then stained with FITC-conjugated antibody to BrdU and 7-AAD, followed by flow cytometry analysis using a BD Accuri instrument (BD Pharmingen). .. For crystal violet staining, cells were seeded at equal numbers and subjected to UVB exposure before being fixed and stained with crystal violet.

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    Becton Dickinson brdu flow kit
    Increased T cell activation in TMEM16F-KO mice during early phase of chronic infection. (A–D) WT or TMEM16F-KO mice were i.v. infected with 4 × 10 6 pfu LCMV clone 13. T cell responses were analyzed on day 6 after infection (A, B, and D) and day 21 after infection (C). (A and B) Frequencies of GP33-tetramer–positive cells among total CD8 T cells in A, and GP66-tetramer–positive cells among total CD4 T cells in B were determined by flow <t>cytometry.</t> n = 4 for WT and n = 5 for KO. (C) Proliferation of GP33-tetramer–positive T cells from spleen was measured by <t>BrdU</t> incorporation at indicated time points after infection. n = 4, 4, 5, and 4, from left to right. (D) Flow cytometry analysis of intracellular IFN-γ production in CD8 T cells in response to GP33, or PMA and Ionomycin (P+I). n = 4 for WT; n = 5 for KO. Results are displayed as mean ± SEM of two independent experiments. *, P
    Brdu Flow Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson brdu
    Intrinsic and extrinsic drivers of NPC proliferation cannot rescue proliferation defects resulting from Baf53a deletion. (a) Photomicrographs of whole brains from BAF53a mutant or control embryos with and without the β-catenin transgene at E15.5. (b) Immunofluorescence staining for the proliferation marker KI67 on E15.5 sagittal sections through the cortex of BAF53a mutant or control embryos with and without the β-catenin transgene, respectively. Scale bar, 100μm . (c) Cell cycle <t>FACS</t> analysis of Actin-CreER ; Baf53a fl/– neurospheres upon <t>BrdU</t> incorporation with and without tamoxifen (Tam) treatment and following application of increasing concentrations of the growth factors bFGF and EGF. (d) Quantification of the mitogens’ effects on cell cycle in the context of Baf53a deletion. n=3, error bars represent SEM, *p
    Brdu, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brdu/product/Becton Dickinson
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    brdu - by Bioz Stars, 2021-05
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    94
    Becton Dickinson fluorescein isothiocyanate conjugated anti brdu antibody
    APA-1 overexpression does not induce senescence. (A) Western blots of transduced HFFs. Mid-life HFFs were infected with concentrated retroviruses expressing empty vector (LXSN), p16, APA-1, or p14 ARF . After selection, lysates were collected and protein concentrations were measured. Then 40 μg of total protein was analyzed by Western blot for expression of p16, APA-1, p14 ARF , p53, p21, and actin. Overexpression of p16, p14 ARF , and APA-1 was confirmed in the corresponding infections. (B) After selection, the cells from A were labeled with 10 μM <t>BrdU</t> for 4 h and fixed in 70% ethanol. Nuclei were labeled with anti-BrdU-fluorescein <t>isothiocyanate</t> and propidium iodide and analyzed by flow cytometry. Percent BrdU-positive cells is presented. (C) The cells from A along with early-passage (HFF PDL 35) and senescent (HFF PDL 75) controls were stained for senescence-associated (SA) β-galactosidase. Percent positive cells is presented.
    Fluorescein Isothiocyanate Conjugated Anti Brdu Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Increased T cell activation in TMEM16F-KO mice during early phase of chronic infection. (A–D) WT or TMEM16F-KO mice were i.v. infected with 4 × 10 6 pfu LCMV clone 13. T cell responses were analyzed on day 6 after infection (A, B, and D) and day 21 after infection (C). (A and B) Frequencies of GP33-tetramer–positive cells among total CD8 T cells in A, and GP66-tetramer–positive cells among total CD4 T cells in B were determined by flow cytometry. n = 4 for WT and n = 5 for KO. (C) Proliferation of GP33-tetramer–positive T cells from spleen was measured by BrdU incorporation at indicated time points after infection. n = 4, 4, 5, and 4, from left to right. (D) Flow cytometry analysis of intracellular IFN-γ production in CD8 T cells in response to GP33, or PMA and Ionomycin (P+I). n = 4 for WT; n = 5 for KO. Results are displayed as mean ± SEM of two independent experiments. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion

    doi: 10.1084/jem.20160612

    Figure Lengend Snippet: Increased T cell activation in TMEM16F-KO mice during early phase of chronic infection. (A–D) WT or TMEM16F-KO mice were i.v. infected with 4 × 10 6 pfu LCMV clone 13. T cell responses were analyzed on day 6 after infection (A, B, and D) and day 21 after infection (C). (A and B) Frequencies of GP33-tetramer–positive cells among total CD8 T cells in A, and GP66-tetramer–positive cells among total CD4 T cells in B were determined by flow cytometry. n = 4 for WT and n = 5 for KO. (C) Proliferation of GP33-tetramer–positive T cells from spleen was measured by BrdU incorporation at indicated time points after infection. n = 4, 4, 5, and 4, from left to right. (D) Flow cytometry analysis of intracellular IFN-γ production in CD8 T cells in response to GP33, or PMA and Ionomycin (P+I). n = 4 for WT; n = 5 for KO. Results are displayed as mean ± SEM of two independent experiments. *, P

    Article Snippet: BrdU incorporation was evaluated by flow cytometry using the BrdU Flow kit (BD) following the manufacturer’s instructions.

    Techniques: Activation Assay, Mouse Assay, Infection, Flow Cytometry, Cytometry, BrdU Incorporation Assay

    Intrinsic and extrinsic drivers of NPC proliferation cannot rescue proliferation defects resulting from Baf53a deletion. (a) Photomicrographs of whole brains from BAF53a mutant or control embryos with and without the β-catenin transgene at E15.5. (b) Immunofluorescence staining for the proliferation marker KI67 on E15.5 sagittal sections through the cortex of BAF53a mutant or control embryos with and without the β-catenin transgene, respectively. Scale bar, 100μm . (c) Cell cycle FACS analysis of Actin-CreER ; Baf53a fl/– neurospheres upon BrdU incorporation with and without tamoxifen (Tam) treatment and following application of increasing concentrations of the growth factors bFGF and EGF. (d) Quantification of the mitogens’ effects on cell cycle in the context of Baf53a deletion. n=3, error bars represent SEM, *p

    Journal: bioRxiv

    Article Title: The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex

    doi: 10.1101/2020.01.17.910794

    Figure Lengend Snippet: Intrinsic and extrinsic drivers of NPC proliferation cannot rescue proliferation defects resulting from Baf53a deletion. (a) Photomicrographs of whole brains from BAF53a mutant or control embryos with and without the β-catenin transgene at E15.5. (b) Immunofluorescence staining for the proliferation marker KI67 on E15.5 sagittal sections through the cortex of BAF53a mutant or control embryos with and without the β-catenin transgene, respectively. Scale bar, 100μm . (c) Cell cycle FACS analysis of Actin-CreER ; Baf53a fl/– neurospheres upon BrdU incorporation with and without tamoxifen (Tam) treatment and following application of increasing concentrations of the growth factors bFGF and EGF. (d) Quantification of the mitogens’ effects on cell cycle in the context of Baf53a deletion. n=3, error bars represent SEM, *p

    Article Snippet: FACS analysisNPC cultures were labeled with BrdU (BD Pharmingen, 559616) for 1 hour or 2 hours, respectively.

    Techniques: Mutagenesis, Immunofluorescence, Staining, Marker, FACS, BrdU Incorporation Assay

    BAF53a regulates cell cycle progression in the developing cortex. (a) Representative coronal sections through the developing mouse brain at E14.5 depicting expression of BAF53a ( top left ) and BAF53b ( bottom left ). Dashed line delineates the wall of the lateral ventricle (LV). Immunofluorescence staining shows BAF53A expression in SOX2+ ( blue ) and TBR2+ ( green ) neural stem and intermediate progenitor cells, respectively ( top right ). Immunolabeling for BAF53b and MAP2 (green, bottom right ) reveals strong BAF53b expression in post-mitotic neurons in the cortical plate at E14.5. Scale bars, 50μm . (b) BAF53a mutant brains at P0. Photomicrographs of whole control, heterozygous and BAF53a mutant brains at P0 ( left ) and Nissl stains of sagittal sections ( right ). Closed arrow points to cerebellum and open arrow indicates the neocortex. (c) Immunoprecipitation and western blot analysis of BAF proteins in control and Baf53a mutant nuclear extracts using an anti-BRG1 antibody ( top panel ). γ-32P labeled-ATP was used as substrate to assess BAF ATPase activity, separated by chromatography and visualized by autoradiography ( bottom panel ). (d) Western blot on BAF53a-deficient and control nuclear glycerol gradients (0-30 %) fractions analyzed for BAF, PRC2 and TIP60 complex components. (e) Growth curve of Actin-CreER ; Baf53a fl/– neurospheres for 6 days after Tamoxifen (Tam) or Ethanol (EtOH) control treatment ( top panel ). Quantification of cell cycle FACS analysis of Actin-CreER ; Baf53a fl/– neurospheres. Cells were treated with BrdU 2 hours before collection and harvested 72 hours after tamoxifen administration for FACS ( bottom panel ). n=3, error bars represent SEM, *p

    Journal: bioRxiv

    Article Title: The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex

    doi: 10.1101/2020.01.17.910794

    Figure Lengend Snippet: BAF53a regulates cell cycle progression in the developing cortex. (a) Representative coronal sections through the developing mouse brain at E14.5 depicting expression of BAF53a ( top left ) and BAF53b ( bottom left ). Dashed line delineates the wall of the lateral ventricle (LV). Immunofluorescence staining shows BAF53A expression in SOX2+ ( blue ) and TBR2+ ( green ) neural stem and intermediate progenitor cells, respectively ( top right ). Immunolabeling for BAF53b and MAP2 (green, bottom right ) reveals strong BAF53b expression in post-mitotic neurons in the cortical plate at E14.5. Scale bars, 50μm . (b) BAF53a mutant brains at P0. Photomicrographs of whole control, heterozygous and BAF53a mutant brains at P0 ( left ) and Nissl stains of sagittal sections ( right ). Closed arrow points to cerebellum and open arrow indicates the neocortex. (c) Immunoprecipitation and western blot analysis of BAF proteins in control and Baf53a mutant nuclear extracts using an anti-BRG1 antibody ( top panel ). γ-32P labeled-ATP was used as substrate to assess BAF ATPase activity, separated by chromatography and visualized by autoradiography ( bottom panel ). (d) Western blot on BAF53a-deficient and control nuclear glycerol gradients (0-30 %) fractions analyzed for BAF, PRC2 and TIP60 complex components. (e) Growth curve of Actin-CreER ; Baf53a fl/– neurospheres for 6 days after Tamoxifen (Tam) or Ethanol (EtOH) control treatment ( top panel ). Quantification of cell cycle FACS analysis of Actin-CreER ; Baf53a fl/– neurospheres. Cells were treated with BrdU 2 hours before collection and harvested 72 hours after tamoxifen administration for FACS ( bottom panel ). n=3, error bars represent SEM, *p

    Article Snippet: FACS analysisNPC cultures were labeled with BrdU (BD Pharmingen, 559616) for 1 hour or 2 hours, respectively.

    Techniques: Expressing, Immunofluorescence, Staining, Immunolabeling, Mutagenesis, Immunoprecipitation, Western Blot, Labeling, Activity Assay, Chromatography, Autoradiography, FACS

    PAF influences DNA replication and pyrimidine metabolism in GSCs. ( A ) Silver-stained gel showing protein interactors of FLAG-PAF in GSC TS543. ( B ) Frequency of GSCs expressing PAF + /PCNA + or PAF + /PCNA − ( n = 5). The gating strategy is shown below. ( C ) Frequency of BrdU + GSCs after PAF depletion ( n = 4) (mean ± SD). 7-AAD, 7-aminoactinomycin D. ( D ) Significantly down-regulated (blue bars) and up-regulated (red bars) pathways identified by GSEA in multiple GSC lines after PAF knockdown (FDR

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: PAF promotes stemness and radioresistance of glioma stem cells

    doi: 10.1073/pnas.1708122114

    Figure Lengend Snippet: PAF influences DNA replication and pyrimidine metabolism in GSCs. ( A ) Silver-stained gel showing protein interactors of FLAG-PAF in GSC TS543. ( B ) Frequency of GSCs expressing PAF + /PCNA + or PAF + /PCNA − ( n = 5). The gating strategy is shown below. ( C ) Frequency of BrdU + GSCs after PAF depletion ( n = 4) (mean ± SD). 7-AAD, 7-aminoactinomycin D. ( D ) Significantly down-regulated (blue bars) and up-regulated (red bars) pathways identified by GSEA in multiple GSC lines after PAF knockdown (FDR

    Article Snippet: To study the cell cycle of GSCs, BrdU was added to cells, and cells were collected after 2 h for staining using a BrdU Flow Kit (BD Pharmingen).

    Techniques: Staining, Expressing

    APA-1 overexpression does not induce senescence. (A) Western blots of transduced HFFs. Mid-life HFFs were infected with concentrated retroviruses expressing empty vector (LXSN), p16, APA-1, or p14 ARF . After selection, lysates were collected and protein concentrations were measured. Then 40 μg of total protein was analyzed by Western blot for expression of p16, APA-1, p14 ARF , p53, p21, and actin. Overexpression of p16, p14 ARF , and APA-1 was confirmed in the corresponding infections. (B) After selection, the cells from A were labeled with 10 μM BrdU for 4 h and fixed in 70% ethanol. Nuclei were labeled with anti-BrdU-fluorescein isothiocyanate and propidium iodide and analyzed by flow cytometry. Percent BrdU-positive cells is presented. (C) The cells from A along with early-passage (HFF PDL 35) and senescent (HFF PDL 75) controls were stained for senescence-associated (SA) β-galactosidase. Percent positive cells is presented.

    Journal: Molecular and Cellular Biology

    Article Title: Induction of Extracellular Matrix-Remodeling Genes by the Senescence-Associated Protein APA-1

    doi: 10.1128/MCB.22.21.7385-7397.2002

    Figure Lengend Snippet: APA-1 overexpression does not induce senescence. (A) Western blots of transduced HFFs. Mid-life HFFs were infected with concentrated retroviruses expressing empty vector (LXSN), p16, APA-1, or p14 ARF . After selection, lysates were collected and protein concentrations were measured. Then 40 μg of total protein was analyzed by Western blot for expression of p16, APA-1, p14 ARF , p53, p21, and actin. Overexpression of p16, p14 ARF , and APA-1 was confirmed in the corresponding infections. (B) After selection, the cells from A were labeled with 10 μM BrdU for 4 h and fixed in 70% ethanol. Nuclei were labeled with anti-BrdU-fluorescein isothiocyanate and propidium iodide and analyzed by flow cytometry. Percent BrdU-positive cells is presented. (C) The cells from A along with early-passage (HFF PDL 35) and senescent (HFF PDL 75) controls were stained for senescence-associated (SA) β-galactosidase. Percent positive cells is presented.

    Article Snippet: Nuclei were isolated from fixed cells, stained with fluorescein isothiocyanate-conjugated anti-BrdU antibody (Becton Dickinson), and resuspended in 50 μg of propidium iodide per ml.

    Techniques: Over Expression, Western Blot, Infection, Expressing, Plasmid Preparation, Selection, Labeling, Flow Cytometry, Cytometry, Staining