Structured Review

PeproTech brain derived neurotrophic factor
Culture of iPSC <t>derived</t> human <t>brain</t> organoids. (A) iPSCs were cultured in essential E8 media (E8) and after a single cell passage differentiated to neuronal progenitor cells (NPCs) in neural induction media (NIM), containing SB431542 and Dorsomorphin. Successful differentiation was assessed, and cells were frozen. Thawed NPCs were cultured and allowed to reach near confluency in neural expansion media (NEM) before being seeded in 96-well ultra-low attachment (ULA) plates. The cells were grown in neural medium (NM), containing hEGF and FGF basic from d10-d15, followed by neural differentiation medium (NDM), containing <t>BDNF</t> and NT3 until the end of culture. On day 25, immature organoids were transferred to 10 cm 2 Petri dishes and kept on an orbital shaker at 65 rpm (B–I) Undifferentiated iPSC colonies (B) were single seeded (C) and differentiated to NPCs (D) . The NPCs were seeded in ULA plates as single cells (E) and after they formed a dense 3D aggregate with a smooth surface and grew in diameter (F) they were transferred to a dynamic culture system and allowed to mature (G–I) . Scale bars represent 500 µm.
Brain Derived Neurotrophic Factor, supplied by PeproTech, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brain derived neurotrophic factor/product/PeproTech
Average 93 stars, based on 40 article reviews
Price from $9.99 to $1999.99
brain derived neurotrophic factor - by Bioz Stars, 2022-12
93/100 stars

Images

1) Product Images from "Induced pluripotent stem cell-derived brain organoids as potential human model system for chemotherapy induced CNS toxicity"

Article Title: Induced pluripotent stem cell-derived brain organoids as potential human model system for chemotherapy induced CNS toxicity

Journal: Frontiers in Molecular Biosciences

doi: 10.3389/fmolb.2022.1006497

Culture of iPSC derived human brain organoids. (A) iPSCs were cultured in essential E8 media (E8) and after a single cell passage differentiated to neuronal progenitor cells (NPCs) in neural induction media (NIM), containing SB431542 and Dorsomorphin. Successful differentiation was assessed, and cells were frozen. Thawed NPCs were cultured and allowed to reach near confluency in neural expansion media (NEM) before being seeded in 96-well ultra-low attachment (ULA) plates. The cells were grown in neural medium (NM), containing hEGF and FGF basic from d10-d15, followed by neural differentiation medium (NDM), containing BDNF and NT3 until the end of culture. On day 25, immature organoids were transferred to 10 cm 2 Petri dishes and kept on an orbital shaker at 65 rpm (B–I) Undifferentiated iPSC colonies (B) were single seeded (C) and differentiated to NPCs (D) . The NPCs were seeded in ULA plates as single cells (E) and after they formed a dense 3D aggregate with a smooth surface and grew in diameter (F) they were transferred to a dynamic culture system and allowed to mature (G–I) . Scale bars represent 500 µm.
Figure Legend Snippet: Culture of iPSC derived human brain organoids. (A) iPSCs were cultured in essential E8 media (E8) and after a single cell passage differentiated to neuronal progenitor cells (NPCs) in neural induction media (NIM), containing SB431542 and Dorsomorphin. Successful differentiation was assessed, and cells were frozen. Thawed NPCs were cultured and allowed to reach near confluency in neural expansion media (NEM) before being seeded in 96-well ultra-low attachment (ULA) plates. The cells were grown in neural medium (NM), containing hEGF and FGF basic from d10-d15, followed by neural differentiation medium (NDM), containing BDNF and NT3 until the end of culture. On day 25, immature organoids were transferred to 10 cm 2 Petri dishes and kept on an orbital shaker at 65 rpm (B–I) Undifferentiated iPSC colonies (B) were single seeded (C) and differentiated to NPCs (D) . The NPCs were seeded in ULA plates as single cells (E) and after they formed a dense 3D aggregate with a smooth surface and grew in diameter (F) they were transferred to a dynamic culture system and allowed to mature (G–I) . Scale bars represent 500 µm.

Techniques Used: Derivative Assay, Cell Culture

2) Product Images from "Anatomical differences in nociceptor neurons sensitivity"

Article Title: Anatomical differences in nociceptor neurons sensitivity

Journal: Bioelectronic Medicine

doi: 10.1186/s42234-022-00088-w

Lumbar and vagal neurons are phenotypically different and respond differently to NGF and BDNF. Na V 1.8 cre ::Td-tomato fl/wt (red) mice JNC ( A, C, E ) and DRG ( B, D, F ) nociceptor neurons were harvested, and cultured (1,500 neurons/plate) for 24h in the presence of vehicle ( A - B ), Nerve Growth Factor (NGF, 50 ng/mL; C - D ), or Brain-Derived Neurotrophic Factor (BDNF, 50 ng/mL; E - F ). In comparison to vehicle, NGF increased neurite outgrowth in cultured DRG neurons, and had limited impact in JNC neurons ( G ). BDNF increased neurite outgrowth in cultured JNC neurons but had no impact in DRG neurons ( G ). In vehicle-exposed culture, JNC neurons have a larger diameter than DRG neurons ( H - I ; n > 1000 neurons). Data are shown as mean ± S.E.M. N=7 dishes/group from two independent experiments ( A - I ). P-values are shown in the figure and determined by one-way ANOVA post hoc Dunnett ( G ) or Mann-Whitney Test ( I ). Scale = 250 μm ( A - F ). NaV1.8 + nociceptors are labeled in red ( A - F )
Figure Legend Snippet: Lumbar and vagal neurons are phenotypically different and respond differently to NGF and BDNF. Na V 1.8 cre ::Td-tomato fl/wt (red) mice JNC ( A, C, E ) and DRG ( B, D, F ) nociceptor neurons were harvested, and cultured (1,500 neurons/plate) for 24h in the presence of vehicle ( A - B ), Nerve Growth Factor (NGF, 50 ng/mL; C - D ), or Brain-Derived Neurotrophic Factor (BDNF, 50 ng/mL; E - F ). In comparison to vehicle, NGF increased neurite outgrowth in cultured DRG neurons, and had limited impact in JNC neurons ( G ). BDNF increased neurite outgrowth in cultured JNC neurons but had no impact in DRG neurons ( G ). In vehicle-exposed culture, JNC neurons have a larger diameter than DRG neurons ( H - I ; n > 1000 neurons). Data are shown as mean ± S.E.M. N=7 dishes/group from two independent experiments ( A - I ). P-values are shown in the figure and determined by one-way ANOVA post hoc Dunnett ( G ) or Mann-Whitney Test ( I ). Scale = 250 μm ( A - F ). NaV1.8 + nociceptors are labeled in red ( A - F )

Techniques Used: Mouse Assay, Cell Culture, Derivative Assay, MANN-WHITNEY, Labeling

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