Journal: International Journal of Microbiology

Article Title: Molecular Confirmation of Vancomycin-Resistant Staphylococcus aureus with vanA Gene from a Hospital in Kathmandu

doi: 10.1155/2021/3847347

Figure Lengend Snippet: Amplification of the vanA gene from S. aureus isolates by conventional PCR (L1, DNA ladder 100–1000 bp; L2-NC, negative control; L3-PC, positive control; L4 to L8, S. aureus isolates; and S22 and S61 isolates were positive for the vanA gene).

Article Snippet: The molecular weight of the amplified product was estimated using a 100 bp DNA ladder (Solis Biodyne, Estonia).

Techniques: Amplification, Negative Control, Positive Control

Journal: Scientific Reports

Article Title: Leishmania donovani persistence and circulation causing cutaneous leishmaniasis in unusual-foci of Nepal

doi: 10.1038/s41598-023-37458-6

Figure Lengend Snippet: Agarose gel electrophoresis visualization of kDNA, ITS1 and ITS1-RFLP. (A1) Minicircle kDNA PCR-1 assay of clinical samples. (A2) Minicircle kDNA PCR-2 assay of clinical samples. PCR 1 amplicons of DNA isolated from the skin biopsies run for gel electrophoresis on 1.5% agarose gel and analysis. B1) ITS1 PCR assay of clinical samples. DNA isolated from the skin biopsies from the lesion was amplified and analyzed on 1.5% agarose gel. B2) ITS1 RFLP assay of ITS1 PCR positive samples. The grouping of the gels cropped were from different gels. The full length gels are included in supplementary information file. The size of the bands are defined as indicated by the arrows. Ladders-100 bp DNA ladder (Solis Biodyne), NC-Negative Control, L.D.- Control of L. donovani (LEM 138), CL- Cutaneous leishmaniasis sample.

Article Snippet: Ladders-100 bp DNA ladder (Solis Biodyne), NC-Negative Control, L.D.- Control of L. donovani (LEM 138), CL- Cutaneous leishmaniasis sample.

Techniques: Agarose Gel Electrophoresis, Isolation, Nucleic Acid Electrophoresis, Amplification, RFLP Assay, Negative Control