bp clonase ii enzyme mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bp clonase ii enzyme mix
    Bp Clonase Ii Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp clonase ii enzyme mix/product/Thermo Fisher
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    bp clonase ii enzyme mix - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: Clones were inoculated overnight at 37°C in Difco Luria-Bertani Broth, Miller (LB; BD Biosciences) in the presence of the appropriate antibiotics, and plasmid DNA was isolated with a Nucleospin plasmid quickpure kit (Macherey-Nagel). .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C.

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: Paragraph title: Cloning and In Vitro Analysis of Candidate Glucosyltransferases. ... The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions.

    Amplification:

    Article Title: A load driver device for engineering modularity in biological networks
    Article Snippet: Nucleic acid manipulation AccuPrime Pfx SuperMix from Life Technologies and oligonucleotides manufactured by Integrated DNA Technologies were used for all PCR amplification. .. BP Clonase II Enzyme Mix and LR Clonase II Plus Enzyme Mix from Life Technologies were used for all BP and LR reactions respectively ( ).

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: Amplified fragments were purified with the Qiagen QIAquick PCR Purification Kit. .. The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: After agarose gel electrophoresis, the appropriate bands were isolated and purified with a Nucleospin Extract II kit (Macherey-Nagel). .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C.

    In Vitro:

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: Paragraph title: Cloning and In Vitro Analysis of Candidate Glucosyltransferases. ... The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions.

    Synthesized:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: RNA was isolated from either cultured OECs or rat cerebellum, and cDNA was synthesized with a QuantiTect Reverse Transcription Kit (QIAGEN). cDNA was amplified by PCR (Phusion DNA polymerase; Finnzymes) using cDNA primers. .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C.

    Isolation:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C. .. The recombination product was transformed in One Shot TOP10 Chemically Competent Escherichia coli (Invitrogen).

    Cell Culture:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: RNA was isolated from either cultured OECs or rat cerebellum, and cDNA was synthesized with a QuantiTect Reverse Transcription Kit (QIAGEN). cDNA was amplified by PCR (Phusion DNA polymerase; Finnzymes) using cDNA primers. .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C.

    Purification:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: After agarose gel electrophoresis, the appropriate bands were isolated and purified with a Nucleospin Extract II kit (Macherey-Nagel). .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C.

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: .. The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions. .. To generate a plasmid with AsTG1 missing the predicted N-terminal signal sequence (AsTG1-NOSIG), 60 ng of the pDONR207-AsTG1 plasmid was used as a template with Fgw-nosigAsTG1 and Rgw-AsTG1 primers (SI Appendix , Table S16 ) by 2-step Gateway cloning.

    Electrophoresis:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C. .. The recombination product was transformed in One Shot TOP10 Chemically Competent Escherichia coli (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: A load driver device for engineering modularity in biological networks
    Article Snippet: Oligonucleotides used in PCR are listed in , used for sequencing are listed in , and used to generate yeast knock-out are listed in . .. BP Clonase II Enzyme Mix and LR Clonase II Plus Enzyme Mix from Life Technologies were used for all BP and LR reactions respectively ( ).

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: Amplified fragments were purified with the Qiagen QIAquick PCR Purification Kit. .. The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions.

    Incubation:

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions. .. Before the PCR purification step, 1 μL of DpnI (New England BioLabs) was added to the amplified PCR product, and the mixture was then incubated at 37 °C for 1 h to digest the pDONR207-AsTG1 DNA template.

    Positron Emission Tomography:

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions. .. Sequence-verified coding sequences were then transferred by LR clonase II into pH9GW (Invitrogen), a Gateway-compatible version of pET-28 encoding 9 N-terminal histidines ( ).

    Construct:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C. .. The LV-destination plasmids containing the candidate genes were constructed by replacing the GFP sequence from the transfer vector pRRLsin-PPthCMV-GFP-wpre with Reading Frame (Rf) Cassette B (RfB) of the Gateway Vector Conversion System (Invitrogen) followed by an IRES-GFP sequence. pDonor plasmids containing validated candidate gene sequences were recombined overnight at 25°C with the LV-destination vector using LR Clonase II (Invitrogen) following gateway manual instructions.

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: Expression constructs were created using Gateway technology (Invitrogen). .. The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions.

    Expressing:

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: Expression constructs were created using Gateway technology (Invitrogen). .. The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions.

    Sequencing:

    Article Title: A load driver device for engineering modularity in biological networks
    Article Snippet: Oligonucleotides used in PCR are listed in , used for sequencing are listed in , and used to generate yeast knock-out are listed in . .. BP Clonase II Enzyme Mix and LR Clonase II Plus Enzyme Mix from Life Technologies were used for all BP and LR reactions respectively ( ).

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions. .. To generate a plasmid with AsTG1 missing the predicted N-terminal signal sequence (AsTG1-NOSIG), 60 ng of the pDONR207-AsTG1 plasmid was used as a template with Fgw-nosigAsTG1 and Rgw-AsTG1 primers (SI Appendix , Table S16 ) by 2-step Gateway cloning.

    Transformation Assay:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C. .. The recombination product was transformed in One Shot TOP10 Chemically Competent Escherichia coli (Invitrogen).

    Knock-Out:

    Article Title: A load driver device for engineering modularity in biological networks
    Article Snippet: Oligonucleotides used in PCR are listed in , used for sequencing are listed in , and used to generate yeast knock-out are listed in . .. BP Clonase II Enzyme Mix and LR Clonase II Plus Enzyme Mix from Life Technologies were used for all BP and LR reactions respectively ( ).

    Plasmid Preparation:

    Article Title: A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons
    Article Snippet: .. After electrophoresis, the appropriate bands were isolated and recombined with the pDONR221 plasmid from the Gateway Vector Conversion System (Invitrogen) using the BP Clonase II enzyme mix overnight at 25°C. .. The recombination product was transformed in One Shot TOP10 Chemically Competent Escherichia coli (Invitrogen).

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat
    Article Snippet: .. The purified CDS fragments were transferred into the pDONR207 vector using BP Clonase II Enzyme Mix (Invitrogen) according to the manufacturer’s instructions. .. To generate a plasmid with AsTG1 missing the predicted N-terminal signal sequence (AsTG1-NOSIG), 60 ng of the pDONR207-AsTG1 plasmid was used as a template with Fgw-nosigAsTG1 and Rgw-AsTG1 primers (SI Appendix , Table S16 ) by 2-step Gateway cloning.

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  • 90
    Thermo Fisher gateway bp clonase ii enzyme mix
    Gateway Bp Clonase Ii Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gateway bp clonase ii enzyme mix/product/Thermo Fisher
    Average 90 stars, based on 155 article reviews
    Price from $9.99 to $1999.99
    gateway bp clonase ii enzyme mix - by Bioz Stars, 2020-02
    90/100 stars
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