bovine serum albumin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bovine serum albumin
    Bovine Serum Albumin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin/product/Thermo Fisher
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin - by Bioz Stars, 2022-10
    99/100 stars

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    Thermo Fisher bsa pbs
    Immunolabeling of TRPV1 in the rat cochlea and dorsal root ganglion (DRG). A : TRPV1 immunolabeling was present in the soma of outer hair cells (OHCs), and inner pillar cells (IPC). B : TRPV1 expression was also identified in the soma of outer pillar cells (OPC), and in C , in Hensen’s cells (HC) and IHCs. D : spiral ganglion neurons (SGN) also expressed TRPV1 labeling. E : immunocytochemical control: negligible labeling was observed in the rat organ of Corti when the TRPV1 primary antibody was replaced by <t>BSA-PBS.</t> F : phalloidin labeling (red) of the organ of Corti shown in E , showing the actiniferous cuticular plate of OHCs (CP, arrow). G : Immunocytochemical control: negligible labeling in the rat organ of Corti was observed when blocking peptide was added to TRPV1 primary antibody incubation media. H : phalloidin labeling of the organ of Corti shown in G . The bar in H represents 50 µm and applies to A–H. I : specific TRPV1 immunolabeling occurs in the cell body of small-diameter DRG neurons. J : immunocytochemical control: negligible labeling in the DRG was observed when the TRPV1 primary antibody was replaced by BSA-PBS. K : immunocytochemical control: negligible labeling in the DRG was observed when blocking peptide was added to TRPV1 primary antibody incubation media. The bar in K represents 100 µm and applies to I–K .
    Bsa Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher dq bsa
    Increased lysosomal proteolytic capacity induced by suppression of AEP activity. a <t>FiTC-BSA</t> uptake and DQ-BSA degradation rates in WT 3T3s treated (red circles) or untreated (blue squares) with 50 μM MVO26630 for 16 h. Data are means ± SEM of n = 8 microscopic fields for untreated and n = 15 microscopic fields for MVO-treated. (Scale bar = 20 μm). b Time course of degradation of oncostatin M receptor beta (Osmrβ) in WT MEFs treated (dashed grey line) or untreated (black solid line) with 50 μM MVO26630 for 16 h and AEP −/− MEFs (red solid line) following receptor downregulation by oncostatin M. Relative intensity of each Osmrβ band was calculated for three independent experiments. c Time course of degradation of epithelial growth factor receptor (EGFR) in WT MEFs treated (dashed grey line) or untreated (black solid line) with 50 μM MVO26630 for 16 h and AEP −/− MEFs (red solid line) following receptor downregulation by epithelial growth factor. Relative intensity of each EGFR band was calculated for three independent experiments. Statistical significance was calculated using a two-sided unpaired t- test. ns = not significant
    Dq Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher rhodamine phalloidin
    DACT1α suppressed cell spreading and F-actin formation ( A ) DACT1α expression was silenced in all gastric cancer cell lines, but expressed in normal human epithelial cell line GES1 and in normal gastric tissues. ( B1 ) Ectopic expression of DACT1α in BGC803, MGC823 and AGS cell lines was confirmed by Western blot (cropped gel). ( B2 ) Knockdown effect of DACT1α by shDACT1α in normal gastric epithelial cell line GES1 was confirmed by Western blot (cropped gel). ( C ) pcDNA3.1-DACT1α vector stably transfected BGC823 and MGC803 cells were significantly slowed the spreading rate on cover slips for 6 hours compared to control vector pcDNA3.1 transfected and BGC823 and MGC803 cells. ( D ) BGC823 and MGC803 cells stained with <t>rhodamine-phalloidin</t> for detecting F-actin formation.
    Rhodamine Phalloidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Immunolabeling of TRPV1 in the rat cochlea and dorsal root ganglion (DRG). A : TRPV1 immunolabeling was present in the soma of outer hair cells (OHCs), and inner pillar cells (IPC). B : TRPV1 expression was also identified in the soma of outer pillar cells (OPC), and in C , in Hensen’s cells (HC) and IHCs. D : spiral ganglion neurons (SGN) also expressed TRPV1 labeling. E : immunocytochemical control: negligible labeling was observed in the rat organ of Corti when the TRPV1 primary antibody was replaced by BSA-PBS. F : phalloidin labeling (red) of the organ of Corti shown in E , showing the actiniferous cuticular plate of OHCs (CP, arrow). G : Immunocytochemical control: negligible labeling in the rat organ of Corti was observed when blocking peptide was added to TRPV1 primary antibody incubation media. H : phalloidin labeling of the organ of Corti shown in G . The bar in H represents 50 µm and applies to A–H. I : specific TRPV1 immunolabeling occurs in the cell body of small-diameter DRG neurons. J : immunocytochemical control: negligible labeling in the DRG was observed when the TRPV1 primary antibody was replaced by BSA-PBS. K : immunocytochemical control: negligible labeling in the DRG was observed when blocking peptide was added to TRPV1 primary antibody incubation media. The bar in K represents 100 µm and applies to I–K .

    Journal: Journal of neurophysiology

    Article Title: Vanilloid Receptors in Hearing: Altered Cochlear Sensitivity by Vanilloids and Expression of TRPV1 in the Organ of Corti

    doi: 10.1152/jn.00919.2002

    Figure Lengend Snippet: Immunolabeling of TRPV1 in the rat cochlea and dorsal root ganglion (DRG). A : TRPV1 immunolabeling was present in the soma of outer hair cells (OHCs), and inner pillar cells (IPC). B : TRPV1 expression was also identified in the soma of outer pillar cells (OPC), and in C , in Hensen’s cells (HC) and IHCs. D : spiral ganglion neurons (SGN) also expressed TRPV1 labeling. E : immunocytochemical control: negligible labeling was observed in the rat organ of Corti when the TRPV1 primary antibody was replaced by BSA-PBS. F : phalloidin labeling (red) of the organ of Corti shown in E , showing the actiniferous cuticular plate of OHCs (CP, arrow). G : Immunocytochemical control: negligible labeling in the rat organ of Corti was observed when blocking peptide was added to TRPV1 primary antibody incubation media. H : phalloidin labeling of the organ of Corti shown in G . The bar in H represents 50 µm and applies to A–H. I : specific TRPV1 immunolabeling occurs in the cell body of small-diameter DRG neurons. J : immunocytochemical control: negligible labeling in the DRG was observed when the TRPV1 primary antibody was replaced by BSA-PBS. K : immunocytochemical control: negligible labeling in the DRG was observed when blocking peptide was added to TRPV1 primary antibody incubation media. The bar in K represents 100 µm and applies to I–K .

    Article Snippet: The organ of Corti and DRG (positive control) of either the guinea pig or rats were dissected and fixed in the solution of 4% paraformaldehyde in 0.02 M PBS for 3 h. The fixed tissues were washed in 0.02 M PBS (pH 7.4), permeabilized with 0.5% Triton X-100 (Sigma) for 1 h and immunoblocked in 10% goat serum in 1% bovine albumin in 0.02 M PBS for 1 h. They were then incubated with rabbit anti-TRPV1 polyclonal antibody (gift from Dr. Caterina) diluted 1:1,000 in 1% BSA-PBS for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated with Alexa-488-conjugated goat anti-rabbit IgG for 3 h (Molecular Probes; 1:100 in 0.02 M PBS).

    Techniques: Immunolabeling, Expressing, Labeling, Blocking Assay, Incubation

    Increased lysosomal proteolytic capacity induced by suppression of AEP activity. a FiTC-BSA uptake and DQ-BSA degradation rates in WT 3T3s treated (red circles) or untreated (blue squares) with 50 μM MVO26630 for 16 h. Data are means ± SEM of n = 8 microscopic fields for untreated and n = 15 microscopic fields for MVO-treated. (Scale bar = 20 μm). b Time course of degradation of oncostatin M receptor beta (Osmrβ) in WT MEFs treated (dashed grey line) or untreated (black solid line) with 50 μM MVO26630 for 16 h and AEP −/− MEFs (red solid line) following receptor downregulation by oncostatin M. Relative intensity of each Osmrβ band was calculated for three independent experiments. c Time course of degradation of epithelial growth factor receptor (EGFR) in WT MEFs treated (dashed grey line) or untreated (black solid line) with 50 μM MVO26630 for 16 h and AEP −/− MEFs (red solid line) following receptor downregulation by epithelial growth factor. Relative intensity of each EGFR band was calculated for three independent experiments. Statistical significance was calculated using a two-sided unpaired t- test. ns = not significant

    Journal: Nature Communications

    Article Title: Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis

    doi: 10.1038/s41467-018-07741-6

    Figure Lengend Snippet: Increased lysosomal proteolytic capacity induced by suppression of AEP activity. a FiTC-BSA uptake and DQ-BSA degradation rates in WT 3T3s treated (red circles) or untreated (blue squares) with 50 μM MVO26630 for 16 h. Data are means ± SEM of n = 8 microscopic fields for untreated and n = 15 microscopic fields for MVO-treated. (Scale bar = 20 μm). b Time course of degradation of oncostatin M receptor beta (Osmrβ) in WT MEFs treated (dashed grey line) or untreated (black solid line) with 50 μM MVO26630 for 16 h and AEP −/− MEFs (red solid line) following receptor downregulation by oncostatin M. Relative intensity of each Osmrβ band was calculated for three independent experiments. c Time course of degradation of epithelial growth factor receptor (EGFR) in WT MEFs treated (dashed grey line) or untreated (black solid line) with 50 μM MVO26630 for 16 h and AEP −/− MEFs (red solid line) following receptor downregulation by epithelial growth factor. Relative intensity of each EGFR band was calculated for three independent experiments. Statistical significance was calculated using a two-sided unpaired t- test. ns = not significant

    Article Snippet: Lysosomal degradation of DQ-BSA WT 3T3 cells (5 × 104 cells) were grown on coverslips and treated with MVO 50 μM for 16 h. Then, cells were incubated with 10 μg/ml of DQ-BSA (Invitrogen) or 50 μg/ml of FiTC-BSA for 2 h at 37 °C, washed twice with PBS, then fixed with 4% paraformaldehyde in PBS for 20 min at room temperature, mounted using Vectashield mounting medium with DAPI (Vector Laboratories) and images were obtained using an LSM 700 confocal microscope (Carl Zeiss).

    Techniques: Activity Assay

    DACT1α suppressed cell spreading and F-actin formation ( A ) DACT1α expression was silenced in all gastric cancer cell lines, but expressed in normal human epithelial cell line GES1 and in normal gastric tissues. ( B1 ) Ectopic expression of DACT1α in BGC803, MGC823 and AGS cell lines was confirmed by Western blot (cropped gel). ( B2 ) Knockdown effect of DACT1α by shDACT1α in normal gastric epithelial cell line GES1 was confirmed by Western blot (cropped gel). ( C ) pcDNA3.1-DACT1α vector stably transfected BGC823 and MGC803 cells were significantly slowed the spreading rate on cover slips for 6 hours compared to control vector pcDNA3.1 transfected and BGC823 and MGC803 cells. ( D ) BGC823 and MGC803 cells stained with rhodamine-phalloidin for detecting F-actin formation.

    Journal: Oncotarget

    Article Title: Dapper homolog 1 alpha suppresses metastasis ability of gastric cancer through inhibiting planar cell polarity pathway

    doi: 10.18632/oncotarget.13234

    Figure Lengend Snippet: DACT1α suppressed cell spreading and F-actin formation ( A ) DACT1α expression was silenced in all gastric cancer cell lines, but expressed in normal human epithelial cell line GES1 and in normal gastric tissues. ( B1 ) Ectopic expression of DACT1α in BGC803, MGC823 and AGS cell lines was confirmed by Western blot (cropped gel). ( B2 ) Knockdown effect of DACT1α by shDACT1α in normal gastric epithelial cell line GES1 was confirmed by Western blot (cropped gel). ( C ) pcDNA3.1-DACT1α vector stably transfected BGC823 and MGC803 cells were significantly slowed the spreading rate on cover slips for 6 hours compared to control vector pcDNA3.1 transfected and BGC823 and MGC803 cells. ( D ) BGC823 and MGC803 cells stained with rhodamine-phalloidin for detecting F-actin formation.

    Article Snippet: The cover slips with spreading cells were fixed with 3% paraformaldehyde (in PBS) at room temperature for 15 min. Then the cells were washed three times with 1 × TBS and permeabilized with 0.1% Triton X-100 at room temperature for 5 min. Then cells were washed three times with 1 × TBS and blocked with 1% BSA at room temperature for 30 min. For F-actin staining, cells were stained with Rhodamine phalloidin (1:40 dilution in 0.2% BSA) (Invitrogen) at 37°C for 20 min in dark.

    Techniques: Expressing, Western Blot, Plasmid Preparation, Stable Transfection, Transfection, Staining