Journal: PLoS ONE
Article Title: The Increased Expression of Integrin ?6 (ITGA6) Enhances Drug Resistance in EVI1high Leukemia
Figure Lengend Snippet: Higher cell adhesion ability in AML cell lines with EVI1 high expression. A . The expression of four integrin genes (ITGB1, ITGB4, ITGA4 and ITGA6), EVI1 and b-actin as a control was determined by semiquantitative RT-PCR in three different EVI1 low and EVI1 high AML cell lines and two primary AML cells lines with high EVI1 expression (PT9 and PT11). B . Six AML cell lines with low or high EVI1expression, as indicated in the figure, were incubated in culture medium on BSA, collagen, fibronectin, laminin or matrigel-coated plates; the percentage of the total number of incubated cells that adhered to the plates was designated as the binding activity (%). Each experiment was performed in triplicate, and the experiments were independently repeated at least three times. The data are given the as the mean ± standard error (S.E). The statistical analysis was performed using the Student's t-test (* p
Article Snippet: A culture plate was coated overnight with a 1/40 dilution of Growth Factor Reduced-Matrigel (BD Falcon), 5 mg/cm2 of fibronectin (BD Falcon), 5 mg/cm2 of laminin (BD Falcon), 0.3 mg/ml of collagen (Nitta Gelatin) or 5% w/v BSA (Nacalai Tesque).
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation, Binding Assay, Activity Assay