Bovine Serum Albumin Gene Bsa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine"
Article Title: Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine
Journal: PLoS ONE
Figure Legend Snippet: Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), BSA (65 kDa), BGN (40 kDa), DCN (38 kDa), COL1 ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).
Techniques Used: Chick Chorioallantoic Membrane Assay, Purification, Western Blot, Labeling, Staining