bovine serum albumin gene bsa  (New England Biolabs)


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    Structured Review

    New England Biolabs bovine serum albumin gene bsa
    Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), <t>BSA</t> (65 kDa), BGN (40 kDa), DCN (38 kDa), <t>COL1</t> ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).
    Bovine Serum Albumin Gene Bsa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin gene bsa/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin gene bsa - by Bioz Stars, 2022-08
    86/100 stars

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    1) Product Images from "Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine"

    Article Title: Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0242376

    Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), BSA (65 kDa), BGN (40 kDa), DCN (38 kDa), COL1 ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).
    Figure Legend Snippet: Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), BSA (65 kDa), BGN (40 kDa), DCN (38 kDa), COL1 ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).

    Techniques Used: Chick Chorioallantoic Membrane Assay, Purification, Western Blot, Labeling, Staining

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    New England Biolabs bovine serum albumin gene bsa
    Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), <t>BSA</t> (65 kDa), BGN (40 kDa), DCN (38 kDa), <t>COL1</t> ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).
    Bovine Serum Albumin Gene Bsa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin gene bsa/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin gene bsa - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 dna ligase buffer
    In vitro assay of DNA flexibility enhancement by HMGB proteins and chimeras. A. Example data from <t>T4</t> DNA ligase cyclization assay for 200-bp DNA probe in the absence (—) and presence of 40 nM HMGB constructs 16 and 5 (see ). B. Graphical
    T4 Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2022-08
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    New England Biolabs dna ligase buffer
    In vitro assay of DNA flexibility enhancement by HMGB proteins and chimeras. A. Example data from <t>T4</t> DNA ligase cyclization assay for 200-bp DNA probe in the absence (—) and presence of 40 nM HMGB constructs 16 and 5 (see ). B. Graphical
    Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ligase buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna ligase buffer - by Bioz Stars, 2022-08
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    Image Search Results


    Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), BSA (65 kDa), BGN (40 kDa), DCN (38 kDa), COL1 ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).

    Journal: PLoS ONE

    Article Title: Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine

    doi: 10.1371/journal.pone.0242376

    Figure Lengend Snippet: Antibodies to CAM-cys selectively target purified proteins. A series of purified proteins were treated in the presence or absence of the non-thiol reductant TCEP (2.5mM) with IAM or NEM (5mM). Proteins were then analyzed by Western blotting with 4E7, 52H11, or OX133. Selected CAM-cys labeled proteins reacted with 4E7 and 52H11. Equal loading of proteins was demonstrated by staining membranes with a non-selective protein stain (Revert, LI-COR). Proteins migrated at the expected molecular masses as follows (approximations): beta casein (27 kDa), BSA (65 kDa), BGN (40 kDa), DCN (38 kDa), COL1 ( > 250 kDa), COL4 ( > 250 kDa), JAG1 (170 kDa), IL17RC (75 kDa), LAMA2 ( > 180 kDa), vWF (260 kDa), TSP1 (150 kDa), TSP2 (155 kDa).

    Article Snippet: Purified proteins were purchased from the following sources (all are human except where noted): Beta casein (bovine), Collagen I (gene:COL1), Collagen IV (Gene:COL4) (Sigma-Aldrich); Bovine serum albumin (gene:BSA) (bovine; New England BioLabs); Biglycan (gene:BGN), Decorin (gene:DCN), Jagged 1 (gene:JAG1), Interleukin 17 receptor C (gene:IL17RC), TSP1 and Thrombospondin-2 ((gene:TSP2) (R & D Systems)), Laminin subunit alpha 2 ((gene:LAMA2) (merosin; Millipore), vWF (Haematologic Technologies, Inc).

    Techniques: Chick Chorioallantoic Membrane Assay, Purification, Western Blot, Labeling, Staining

    In vitro assay of DNA flexibility enhancement by HMGB proteins and chimeras. A. Example data from T4 DNA ligase cyclization assay for 200-bp DNA probe in the absence (—) and presence of 40 nM HMGB constructs 16 and 5 (see ). B. Graphical

    Journal: Biochemistry

    Article Title: Enhancement of DNA flexibility in vitro and in vivo by HMGB box A proteins carrying box B residues

    doi: 10.1021/bi802269f

    Figure Lengend Snippet: In vitro assay of DNA flexibility enhancement by HMGB proteins and chimeras. A. Example data from T4 DNA ligase cyclization assay for 200-bp DNA probe in the absence (—) and presence of 40 nM HMGB constructs 16 and 5 (see ). B. Graphical

    Article Snippet: DNA cyclization reactions (10 μL) were performed with 10 nM DNA probe and the indicated concentration of HMGB protein in T4 DNA ligase buffer (20 mM Tris-HCl pH 8.0, 30 mM KCl, 100 μg/mL BSA, 1.8 mM ATP, 10 mM MgCl2 ), and T4 DNA ligase (20-50 U/μL; New England Biolabs) at 22°C.

    Techniques: In Vitro, Construct