bovine recombinant il 17a  (Kingfisher Biotech)


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    Structured Review

    Kingfisher Biotech bovine recombinant il 17a
    Effects of CpG oligodinucleotide, <t>interleukin-17A</t> and interferon-α on tracheal antimicrobial peptide gene expression. Dose- and time-dependent effects were measured for CpG oligodinucleotide (A,B) , a TLR9 agonist; interleukin-17A (C,D) ; and interferon-α (E,F) . For the dose–response studies (A,C,E) , confluent bTEC were stimulated in triplicate with various concentrations of agonist for 16 h. For the time-course studies (B,D,F) , confluent bTEC were stimulated in triplicate for 4, 8, 16 or 24 h with the doses of agonist shown. Gene expression of TAP relative to that of GAPDH was measured using real-time RT-qPCR. Lipopolysaccharide (LPS, 0.1 μg/mL) was used in all assays as a positive control and standard. *, significantly different from unstimulated cells ( P
    Bovine Recombinant Il 17a, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine recombinant il 17a/product/Kingfisher Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine recombinant il 17a - by Bioz Stars, 2022-12
    86/100 stars

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    1) Product Images from "Comparison of innate immune agonists for induction of tracheal antimicrobial peptide gene expression in tracheal epithelial cells of cattle"

    Article Title: Comparison of innate immune agonists for induction of tracheal antimicrobial peptide gene expression in tracheal epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-014-0105-8

    Effects of CpG oligodinucleotide, interleukin-17A and interferon-α on tracheal antimicrobial peptide gene expression. Dose- and time-dependent effects were measured for CpG oligodinucleotide (A,B) , a TLR9 agonist; interleukin-17A (C,D) ; and interferon-α (E,F) . For the dose–response studies (A,C,E) , confluent bTEC were stimulated in triplicate with various concentrations of agonist for 16 h. For the time-course studies (B,D,F) , confluent bTEC were stimulated in triplicate for 4, 8, 16 or 24 h with the doses of agonist shown. Gene expression of TAP relative to that of GAPDH was measured using real-time RT-qPCR. Lipopolysaccharide (LPS, 0.1 μg/mL) was used in all assays as a positive control and standard. *, significantly different from unstimulated cells ( P
    Figure Legend Snippet: Effects of CpG oligodinucleotide, interleukin-17A and interferon-α on tracheal antimicrobial peptide gene expression. Dose- and time-dependent effects were measured for CpG oligodinucleotide (A,B) , a TLR9 agonist; interleukin-17A (C,D) ; and interferon-α (E,F) . For the dose–response studies (A,C,E) , confluent bTEC were stimulated in triplicate with various concentrations of agonist for 16 h. For the time-course studies (B,D,F) , confluent bTEC were stimulated in triplicate for 4, 8, 16 or 24 h with the doses of agonist shown. Gene expression of TAP relative to that of GAPDH was measured using real-time RT-qPCR. Lipopolysaccharide (LPS, 0.1 μg/mL) was used in all assays as a positive control and standard. *, significantly different from unstimulated cells ( P

    Techniques Used: Expressing, Quantitative RT-PCR, Positive Control

    Comparison of the effects of IL-17A, Pam3CSK4 and LPS on tracheal antimicrobial peptide gene expression. Confluent cultures of tracheal epithelial cells from 4 different calves were non-stimulated (NS) or stimulated with 1 μg/mL Pam3CSK4, 316 ng/mL IL-17A, or 0.1 μg/mL LPS for 8 h (A) and 16 h (B) in triplicate. Gene expression was assessed using real-time RT-qPCR. Pam3CSK4 induced significantly higher tracheal antimicrobial peptide gene expression than IL-17A and LPS at both 8 and 16 h ( P
    Figure Legend Snippet: Comparison of the effects of IL-17A, Pam3CSK4 and LPS on tracheal antimicrobial peptide gene expression. Confluent cultures of tracheal epithelial cells from 4 different calves were non-stimulated (NS) or stimulated with 1 μg/mL Pam3CSK4, 316 ng/mL IL-17A, or 0.1 μg/mL LPS for 8 h (A) and 16 h (B) in triplicate. Gene expression was assessed using real-time RT-qPCR. Pam3CSK4 induced significantly higher tracheal antimicrobial peptide gene expression than IL-17A and LPS at both 8 and 16 h ( P

    Techniques Used: Expressing, Quantitative RT-PCR

    Effect of stimulation with single agonists compared to combined agonists. Cultured bovine tracheal epithelial cells were stimulated for 16 h in triplicate with various combinations of 1 μg/mL Pam3CSK4, 316 ng/mL IL-17A, or 0.1 μg/mL LPS. Tracheal antimicrobial peptide gene expression was measured as above. The effects of combined agonists were greater than that of interleukin-17A (IL-17A) alone, but minimally or not different than that of lipopolysaccharide (LPS) or Pam3CSK4 alone. The data shown (panels A, B and C) represent 3 studies conducted on different days using cells from different calves.
    Figure Legend Snippet: Effect of stimulation with single agonists compared to combined agonists. Cultured bovine tracheal epithelial cells were stimulated for 16 h in triplicate with various combinations of 1 μg/mL Pam3CSK4, 316 ng/mL IL-17A, or 0.1 μg/mL LPS. Tracheal antimicrobial peptide gene expression was measured as above. The effects of combined agonists were greater than that of interleukin-17A (IL-17A) alone, but minimally or not different than that of lipopolysaccharide (LPS) or Pam3CSK4 alone. The data shown (panels A, B and C) represent 3 studies conducted on different days using cells from different calves.

    Techniques Used: Cell Culture, Expressing

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    Kingfisher Biotech recombinant protein
    Vaccination with rBCG-N-hRSV increases virus and BCG-specific IFN-γ and IL-17 secretion in PBMC cultures. (A, C) Study 1 PBMCs and (B, D) Study 2 Tracheobronchial lymph node cells (TBLNs) were isolated on day 7 post-infection, labeled with Cell Trace Violet, and restimulated in vitro with PPD-B, Ag85A/TB10.4, N-hRSV or bRSV strain 375. Mock stimulated cultures were used as negative controls. ConA stimulated cultures were used as positive controls (not shown). Six days later, cell culture supernatants were analyzed for bovine IFN-γ and <t>IL-17A</t> secretion using commercial ELISA kits. *p
    Recombinant Protein, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant protein/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant protein - by Bioz Stars, 2022-12
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    86
    Kingfisher Biotech polyclonal rabbit anti bovine il 17a antibodies
    Vaccination with rBCG-N-hRSV increases virus and BCG-specific IFN-γ and IL-17 secretion in PBMC cultures. (A, C) Study 1 PBMCs and (B, D) Study 2 Tracheobronchial lymph node cells (TBLNs) were isolated on day 7 post-infection, labeled with Cell Trace Violet, and restimulated in vitro with PPD-B, Ag85A/TB10.4, N-hRSV or bRSV strain 375. Mock stimulated cultures were used as negative controls. ConA stimulated cultures were used as positive controls (not shown). Six days later, cell culture supernatants were analyzed for bovine IFN-γ and <t>IL-17A</t> secretion using commercial ELISA kits. *p
    Polyclonal Rabbit Anti Bovine Il 17a Antibodies, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti bovine il 17a antibodies/product/Kingfisher Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti bovine il 17a antibodies - by Bioz Stars, 2022-12
    86/100 stars
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    Vaccination with rBCG-N-hRSV increases virus and BCG-specific IFN-γ and IL-17 secretion in PBMC cultures. (A, C) Study 1 PBMCs and (B, D) Study 2 Tracheobronchial lymph node cells (TBLNs) were isolated on day 7 post-infection, labeled with Cell Trace Violet, and restimulated in vitro with PPD-B, Ag85A/TB10.4, N-hRSV or bRSV strain 375. Mock stimulated cultures were used as negative controls. ConA stimulated cultures were used as positive controls (not shown). Six days later, cell culture supernatants were analyzed for bovine IFN-γ and IL-17A secretion using commercial ELISA kits. *p

    Journal: Frontiers in Immunology

    Article Title: A Recombinant BCG Vaccine Is Safe and Immunogenic in Neonatal Calves and Reduces the Clinical Disease Caused by the Respiratory Syncytial Virus

    doi: 10.3389/fimmu.2021.664212

    Figure Lengend Snippet: Vaccination with rBCG-N-hRSV increases virus and BCG-specific IFN-γ and IL-17 secretion in PBMC cultures. (A, C) Study 1 PBMCs and (B, D) Study 2 Tracheobronchial lymph node cells (TBLNs) were isolated on day 7 post-infection, labeled with Cell Trace Violet, and restimulated in vitro with PPD-B, Ag85A/TB10.4, N-hRSV or bRSV strain 375. Mock stimulated cultures were used as negative controls. ConA stimulated cultures were used as positive controls (not shown). Six days later, cell culture supernatants were analyzed for bovine IFN-γ and IL-17A secretion using commercial ELISA kits. *p

    Article Snippet: ELISAs Bovine IL-17A and IFNγ were quantified using commercial bovine kits according to the instructions provided by manufacturer (Kingfisher Biotech, Inc).

    Techniques: Isolation, Infection, Labeling, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay