genechip bovine genome 1 0 st array  (Thermo Fisher)


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    Structured Review

    Thermo Fisher genechip bovine genome 1 0 st array
    Composition of the 1470 Affymetrix <t>Genechip</t> Bovine Genome Arrays used in this study. Arrays were classified according to the experimental conditions ( a ) and distribution ( b )
    Genechip Bovine Genome 1 0 St Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genechip bovine genome 1 0 st array/product/Thermo Fisher
    Average 94 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    genechip bovine genome 1 0 st array - by Bioz Stars, 2022-11
    94/100 stars

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    1) Product Images from "Large-scale gene co-expression network as a source of functional annotation for cattle genes"

    Article Title: Large-scale gene co-expression network as a source of functional annotation for cattle genes

    Journal: BMC Genomics

    doi: 10.1186/s12864-016-3176-2

    Composition of the 1470 Affymetrix Genechip Bovine Genome Arrays used in this study. Arrays were classified according to the experimental conditions ( a ) and distribution ( b )
    Figure Legend Snippet: Composition of the 1470 Affymetrix Genechip Bovine Genome Arrays used in this study. Arrays were classified according to the experimental conditions ( a ) and distribution ( b )

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    Thermo Fisher genechip bovine genome 1 0 st array
    Genechip Bovine Genome 1 0 St Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genechip bovine genome 1 0 st array/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    genechip bovine genome 1 0 st array - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    90
    Thermo Fisher wilms tumor protein
    Mesothelial cells are a VAT-specific cell type. (A) UMAP plot of ME across visceral (VAT) and subcutaneous (SAT) adipose tissue of dairy cows (n = 3) obtained through snRNA-seq analysis. (B) Violin plots of ME marker genes MSLN, KRT19, <t>WT1</t> and UPK3B in ME subtypes. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (C) UMAP plots of VAT and SAT ME. (D) Violin plots of ME marker genes in SAT and VAT. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (E) Comparison of adipocyte relative to preadipocyte gene expression fold change of ME marker genes ( MSLN, UPK3B, and WT1 ) between SAT and VAT (n = 4). For gene expression fold change calculations, adipocyte MSLN, UPK3B, and WT1 expression were calibrated by preadipocyte expression in matched SAT and VAT samples from each cow. (F) Immunofluorescence imaging of (1) SAT and (2) VAT SVF stained with LUM (green), WT1 (red), and DAPI (blue) (×20 objective, 100 µm scale bars), and (3) SAT and (4) VAT whole tissue (×4 objective, 1,000 µm scale bars) stained with WT1 (red), and DAPI (blue). Red arrows highlight WT1+ cells. (G) Flow cytometry analysis of SAT and VAT SVF from an indepen dent cohort of dairy cows (n = 10) showing percentages of (from left to right): Total ME cells (MSLN+); ME cells (MSLN+) in ASPC (CD31 − CD45 − ) population; CD31 + cells that do not express MSLN; CD31 + cells that express MSLN; and ME cells (MSLN+) that do not express CD31. * p value ≤ 0.05 and; *** p value ≤ 0.0001 using paired t -test.
    Wilms Tumor Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher anti ugdh rabbit polyclonal antibody
    Mesothelial cells are a VAT-specific cell type. (A) UMAP plot of ME across visceral (VAT) and subcutaneous (SAT) adipose tissue of dairy cows (n = 3) obtained through snRNA-seq analysis. (B) Violin plots of ME marker genes MSLN, KRT19, <t>WT1</t> and UPK3B in ME subtypes. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (C) UMAP plots of VAT and SAT ME. (D) Violin plots of ME marker genes in SAT and VAT. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (E) Comparison of adipocyte relative to preadipocyte gene expression fold change of ME marker genes ( MSLN, UPK3B, and WT1 ) between SAT and VAT (n = 4). For gene expression fold change calculations, adipocyte MSLN, UPK3B, and WT1 expression were calibrated by preadipocyte expression in matched SAT and VAT samples from each cow. (F) Immunofluorescence imaging of (1) SAT and (2) VAT SVF stained with LUM (green), WT1 (red), and DAPI (blue) (×20 objective, 100 µm scale bars), and (3) SAT and (4) VAT whole tissue (×4 objective, 1,000 µm scale bars) stained with WT1 (red), and DAPI (blue). Red arrows highlight WT1+ cells. (G) Flow cytometry analysis of SAT and VAT SVF from an indepen dent cohort of dairy cows (n = 10) showing percentages of (from left to right): Total ME cells (MSLN+); ME cells (MSLN+) in ASPC (CD31 − CD45 − ) population; CD31 + cells that do not express MSLN; CD31 + cells that express MSLN; and ME cells (MSLN+) that do not express CD31. * p value ≤ 0.05 and; *** p value ≤ 0.0001 using paired t -test.
    Anti Ugdh Rabbit Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ugdh rabbit polyclonal antibody/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ugdh rabbit polyclonal antibody - by Bioz Stars, 2022-11
    94/100 stars
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    Mesothelial cells are a VAT-specific cell type. (A) UMAP plot of ME across visceral (VAT) and subcutaneous (SAT) adipose tissue of dairy cows (n = 3) obtained through snRNA-seq analysis. (B) Violin plots of ME marker genes MSLN, KRT19, WT1 and UPK3B in ME subtypes. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (C) UMAP plots of VAT and SAT ME. (D) Violin plots of ME marker genes in SAT and VAT. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (E) Comparison of adipocyte relative to preadipocyte gene expression fold change of ME marker genes ( MSLN, UPK3B, and WT1 ) between SAT and VAT (n = 4). For gene expression fold change calculations, adipocyte MSLN, UPK3B, and WT1 expression were calibrated by preadipocyte expression in matched SAT and VAT samples from each cow. (F) Immunofluorescence imaging of (1) SAT and (2) VAT SVF stained with LUM (green), WT1 (red), and DAPI (blue) (×20 objective, 100 µm scale bars), and (3) SAT and (4) VAT whole tissue (×4 objective, 1,000 µm scale bars) stained with WT1 (red), and DAPI (blue). Red arrows highlight WT1+ cells. (G) Flow cytometry analysis of SAT and VAT SVF from an indepen dent cohort of dairy cows (n = 10) showing percentages of (from left to right): Total ME cells (MSLN+); ME cells (MSLN+) in ASPC (CD31 − CD45 − ) population; CD31 + cells that do not express MSLN; CD31 + cells that express MSLN; and ME cells (MSLN+) that do not express CD31. * p value ≤ 0.05 and; *** p value ≤ 0.0001 using paired t -test.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows

    doi: 10.3389/fcell.2022.1025240

    Figure Lengend Snippet: Mesothelial cells are a VAT-specific cell type. (A) UMAP plot of ME across visceral (VAT) and subcutaneous (SAT) adipose tissue of dairy cows (n = 3) obtained through snRNA-seq analysis. (B) Violin plots of ME marker genes MSLN, KRT19, WT1 and UPK3B in ME subtypes. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (C) UMAP plots of VAT and SAT ME. (D) Violin plots of ME marker genes in SAT and VAT. The y axis indicates log-transformed expression values, and the width indicates the number of cells expressing the gene. (E) Comparison of adipocyte relative to preadipocyte gene expression fold change of ME marker genes ( MSLN, UPK3B, and WT1 ) between SAT and VAT (n = 4). For gene expression fold change calculations, adipocyte MSLN, UPK3B, and WT1 expression were calibrated by preadipocyte expression in matched SAT and VAT samples from each cow. (F) Immunofluorescence imaging of (1) SAT and (2) VAT SVF stained with LUM (green), WT1 (red), and DAPI (blue) (×20 objective, 100 µm scale bars), and (3) SAT and (4) VAT whole tissue (×4 objective, 1,000 µm scale bars) stained with WT1 (red), and DAPI (blue). Red arrows highlight WT1+ cells. (G) Flow cytometry analysis of SAT and VAT SVF from an indepen dent cohort of dairy cows (n = 10) showing percentages of (from left to right): Total ME cells (MSLN+); ME cells (MSLN+) in ASPC (CD31 − CD45 − ) population; CD31 + cells that do not express MSLN; CD31 + cells that express MSLN; and ME cells (MSLN+) that do not express CD31. * p value ≤ 0.05 and; *** p value ≤ 0.0001 using paired t -test.

    Article Snippet: For preadipocytes, cells were stained for lumican (LUM; Invitrogen, Cat. No. MA5-34828; 1:200 antibody: 0.2% BSA-PBS) and Wilms tumor protein (WT1, Invitrogen; Cat. No. MA5-38660; 1:500 antibody: 0.2% BSA-PBS).

    Techniques: Marker, Transformation Assay, Expressing, Immunofluorescence, Imaging, Staining, Flow Cytometry

    Single-nuclei RNA sequencing (snRNA-seq) analysis in subcutaneous (SAT) and visceral (VAT) adipose tissue samples from three dairy cows (n = 3). (A) UMAP plot of nuclei subpopulations in combined VAT and SAT samples from dairy cows. Cell populations were classified as mature adipocytes (AD), adipose steam and progenitor cells (ASPC), endothelial cells (EC), mesothelial cells (ME), pericytes and smooth muscle cells (PC/SMC), macrophages/monocytes (MAC), and natural killer and T-cells (NKT). (B) Heatmap of marker genes expression in cell subtypes identified by snRNA-seq. Color encodes the scaled average expression level across those cells (dark red: high expression; blue: downregulation). (C) UMAP plots of signature genes for ME ( MSLN and WT1 ), AD ( ADIPOQ and LEP ), ASPC ( PPARG and PDGFRA ), EC ( VWF ), MAC ( CD163 and MRC1 ), NKT ( CD52 and CD3E ), PC/SMC ( NOTCH3 ). Purple dots represent individual nucleus expressing the respective marker. (D) Percentages of nuclei per cell type across SAT and VAT. (E) Integrated analysis of cell types identified by our database (CSB Lab-Bovine) compared with human scRNAseq data generated by Merrick et al. (2019) (Seale Lab - Human). (F) Integrated analysis of cell types identified by our database (CSB Lab-Bovine) compared with human scRNAseq data generated by Vijay et al. (2020) (Grundberg Lab – Human). Bar graph represents the comparison between the proportions of distinct cell types found in each study.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows

    doi: 10.3389/fcell.2022.1025240

    Figure Lengend Snippet: Single-nuclei RNA sequencing (snRNA-seq) analysis in subcutaneous (SAT) and visceral (VAT) adipose tissue samples from three dairy cows (n = 3). (A) UMAP plot of nuclei subpopulations in combined VAT and SAT samples from dairy cows. Cell populations were classified as mature adipocytes (AD), adipose steam and progenitor cells (ASPC), endothelial cells (EC), mesothelial cells (ME), pericytes and smooth muscle cells (PC/SMC), macrophages/monocytes (MAC), and natural killer and T-cells (NKT). (B) Heatmap of marker genes expression in cell subtypes identified by snRNA-seq. Color encodes the scaled average expression level across those cells (dark red: high expression; blue: downregulation). (C) UMAP plots of signature genes for ME ( MSLN and WT1 ), AD ( ADIPOQ and LEP ), ASPC ( PPARG and PDGFRA ), EC ( VWF ), MAC ( CD163 and MRC1 ), NKT ( CD52 and CD3E ), PC/SMC ( NOTCH3 ). Purple dots represent individual nucleus expressing the respective marker. (D) Percentages of nuclei per cell type across SAT and VAT. (E) Integrated analysis of cell types identified by our database (CSB Lab-Bovine) compared with human scRNAseq data generated by Merrick et al. (2019) (Seale Lab - Human). (F) Integrated analysis of cell types identified by our database (CSB Lab-Bovine) compared with human scRNAseq data generated by Vijay et al. (2020) (Grundberg Lab – Human). Bar graph represents the comparison between the proportions of distinct cell types found in each study.

    Article Snippet: For preadipocytes, cells were stained for lumican (LUM; Invitrogen, Cat. No. MA5-34828; 1:200 antibody: 0.2% BSA-PBS) and Wilms tumor protein (WT1, Invitrogen; Cat. No. MA5-38660; 1:500 antibody: 0.2% BSA-PBS).

    Techniques: RNA Sequencing Assay, Serial Time-encoded Amplified Microscopy, Marker, Expressing, Generated