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FVIII -/- mice suffer from osteoporosis and show altered bone vascularization with reduced osteoblastic cells. a Bone parameters, X-ray tomodensitometry analysis. Bone density images were obtained using the DataViewer software (Bruker) and represent sagittal, coronal, and transversal views of femurs. Parameters were analyzed at the growth plate level of the distal epiphysis of the femurs (metaphyseal). The same point of reference (chrondocyte seam) at the growth plate was established for each sample, from an offset of 0.215 mm from the growth plate and a trabecular volume of 1.5 mm 3 was selected for the analysis of bone <t>morphometric</t> parameters. b Bone vascularization parameters analyzed from trabecular bone sections (Endomucin in green, NG2 in purple, α- SMA in red, Dapi in blue). Light grey: WT mice, dark grey: FVIII -/- mice, BMD bone mineral density, BV/TV bone volume/total volume, Tb.Th trabecular thickness, Tb. N trabecular number, Tb.Sp trabecular space, Cor coronal, Sag sagittal, Trans transversal, up to 11 mice per group, one to four sections per bone analyzed. Mice were 5 (juvenile) or 14 (young adult) weeks old at sacrifice. * P < 0.05, ** P < 0.01, *** P < 0.001, ns not significant. Statistical test used: non-parametric Mann-Whitney test. Mean ± SD
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Average 99 stars, based on 1 article reviews
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threedimensional bone morphometric parameters  (Skyscan Corporation)
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FVIII -/- mice suffer from osteoporosis and show altered bone vascularization with reduced osteoblastic cells. a Bone parameters, X-ray tomodensitometry analysis. Bone density images were obtained using the DataViewer software (Bruker) and represent sagittal, coronal, and transversal views of femurs. Parameters were analyzed at the growth plate level of the distal epiphysis of the femurs (metaphyseal). The same point of reference (chrondocyte seam) at the growth plate was established for each sample, from an offset of 0.215 mm from the growth plate and a trabecular volume of 1.5 mm 3 was selected for the analysis of bone <t>morphometric</t> parameters. b Bone vascularization parameters analyzed from trabecular bone sections (Endomucin in green, NG2 in purple, α- SMA in red, Dapi in blue). Light grey: WT mice, dark grey: FVIII -/- mice, BMD bone mineral density, BV/TV bone volume/total volume, Tb.Th trabecular thickness, Tb. N trabecular number, Tb.Sp trabecular space, Cor coronal, Sag sagittal, Trans transversal, up to 11 mice per group, one to four sections per bone analyzed. Mice were 5 (juvenile) or 14 (young adult) weeks old at sacrifice. * P < 0.05, ** P < 0.01, *** P < 0.001, ns not significant. Statistical test used: non-parametric Mann-Whitney test. Mean ± SD
Threedimensional Bone Morphometric Parameters, supplied by Skyscan Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FVIII -/- mice suffer from osteoporosis and show altered bone vascularization with reduced osteoblastic cells. a Bone parameters, X-ray tomodensitometry analysis. Bone density images were obtained using the DataViewer software (Bruker) and represent sagittal, coronal, and transversal views of femurs. Parameters were analyzed at the growth plate level of the distal epiphysis of the femurs (metaphyseal). The same point of reference (chrondocyte seam) at the growth plate was established for each sample, from an offset of 0.215 mm from the growth plate and a trabecular volume of 1.5 mm 3 was selected for the analysis of bone morphometric parameters. b Bone vascularization parameters analyzed from trabecular bone sections (Endomucin in green, NG2 in purple, α- SMA in red, Dapi in blue). Light grey: WT mice, dark grey: FVIII -/- mice, BMD bone mineral density, BV/TV bone volume/total volume, Tb.Th trabecular thickness, Tb. N trabecular number, Tb.Sp trabecular space, Cor coronal, Sag sagittal, Trans transversal, up to 11 mice per group, one to four sections per bone analyzed. Mice were 5 (juvenile) or 14 (young adult) weeks old at sacrifice. * P < 0.05, ** P < 0.01, *** P < 0.001, ns not significant. Statistical test used: non-parametric Mann-Whitney test. Mean ± SD

Journal: Bone Research

Article Title: Factor VIII restores bone parameters and modulates muscle proteo-metabolome in Factor VIII knockout male mice

doi: 10.1038/s41413-025-00485-2

Figure Lengend Snippet: FVIII -/- mice suffer from osteoporosis and show altered bone vascularization with reduced osteoblastic cells. a Bone parameters, X-ray tomodensitometry analysis. Bone density images were obtained using the DataViewer software (Bruker) and represent sagittal, coronal, and transversal views of femurs. Parameters were analyzed at the growth plate level of the distal epiphysis of the femurs (metaphyseal). The same point of reference (chrondocyte seam) at the growth plate was established for each sample, from an offset of 0.215 mm from the growth plate and a trabecular volume of 1.5 mm 3 was selected for the analysis of bone morphometric parameters. b Bone vascularization parameters analyzed from trabecular bone sections (Endomucin in green, NG2 in purple, α- SMA in red, Dapi in blue). Light grey: WT mice, dark grey: FVIII -/- mice, BMD bone mineral density, BV/TV bone volume/total volume, Tb.Th trabecular thickness, Tb. N trabecular number, Tb.Sp trabecular space, Cor coronal, Sag sagittal, Trans transversal, up to 11 mice per group, one to four sections per bone analyzed. Mice were 5 (juvenile) or 14 (young adult) weeks old at sacrifice. * P < 0.05, ** P < 0.01, *** P < 0.001, ns not significant. Statistical test used: non-parametric Mann-Whitney test. Mean ± SD

Article Snippet: Bone morphometric parameters were obtained using a Skyscan 1276 CMOS in vivo micro-CT scanner (Bruker, Kontich, Belgium).

Techniques: Software, MANN-WHITNEY