Article Title: Endothelin-1 stimulates human colonic myofibroblast contraction and migration
Figure Lengend Snippet: Effect of endothelin-1 (ET-1) on migration by colonic myofibroblasts. (A) ET-1 stimulated migration in a dose dependent manner. Migration is presented as the reduction in wound area at 24 hours as a percentage of the maximal wound area (that is, initial quantitation). Each data point represents the mean (SEM) (n=5 for each point). (B) Migration in response to 10 nM sarafotoxin (SFTX) or 10 nM ET-1 was measured at 24 hours in the presence or absence of 10 μM BQ-123 (ETA-R inhib) or 10 μM BQ-788 (ETB-R inhib), as indicated. Each bar represents the mean (SEM) (n=5). ET-1 stimulated migration was significantly (p
Article Snippet: Interleukin 1α (IL-1α), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), tumour necrosis factor α (TNF-α), platelet derived growth factor-BB (PDGF-BB), endothelin-1, sarafotoxin, BQ-123, and BQ-788 were obtained from Calbiochem (LaJolla, California, USA) and salts, buffers, and other chemicals from Sigma (St Louis, Missouri, USA).
Techniques: Migration, Quantitation Assay, Inhibition