bmscs  (Millipore)

 
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    Name:
    Rat Marrow Stromal Cells
    Description:
    Bone marrow is the major blood creating organ but in addition to supporting hematopoietic growth and differentiation marrow stromal cells can be induced to produce cells of other connective tissues such as bone cartilage and fat as well as cells from neuroectodermal neurons and endodermal hepatocytes lineages The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell based therapy in the restoration of damaged or diseased tissue RMSC from Cell Applications Inc have been used to demonstrate that TGF beta stimulates production of MCP 1 by vascular smooth muscle cells which attracts bone marrow stromal cells Zhang 2009 and show that their migration can be stimulated by VPA and lithium through HDAC CXCR4 and GSK 3beta MMP 9 respectively Tsai 2010 to investigate the therapeutic potential of marrow stromal stem cells depending on the extracellular matrix properties Gershlak 2013 Sullivan 2014 and demonstrate that combination of low level laser therapy and transplantation of marrow stromal stem cells results in greater functional recovery after nerve crush injury Yang 2013 Finally HMSC were used to develop methods to direct stem cell differentiation by altering physical topography of the substrate Brammer 2011 and to design surface materials to control cell adhesion properties Sommani 2010 Tsuji 2011
    Catalog Number:
    R492-05A
    Price:
    None
    Applications:
    Blood production, hematopoietic growth and differentiation, pathways of pluripotent cell differentiation into bone, cartilage and fat, connective tissue production,supportive structure, cell production, cell proliferation, cell based therapy, tissue restoration and repair, differentiation, effects of culture conditinos, HLA expression, cytokine production, immunomodulation, clinical trials.
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    Structured Review

    Millipore bmscs
    Rat Marrow Stromal Cells
    Bone marrow is the major blood creating organ but in addition to supporting hematopoietic growth and differentiation marrow stromal cells can be induced to produce cells of other connective tissues such as bone cartilage and fat as well as cells from neuroectodermal neurons and endodermal hepatocytes lineages The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell based therapy in the restoration of damaged or diseased tissue RMSC from Cell Applications Inc have been used to demonstrate that TGF beta stimulates production of MCP 1 by vascular smooth muscle cells which attracts bone marrow stromal cells Zhang 2009 and show that their migration can be stimulated by VPA and lithium through HDAC CXCR4 and GSK 3beta MMP 9 respectively Tsai 2010 to investigate the therapeutic potential of marrow stromal stem cells depending on the extracellular matrix properties Gershlak 2013 Sullivan 2014 and demonstrate that combination of low level laser therapy and transplantation of marrow stromal stem cells results in greater functional recovery after nerve crush injury Yang 2013 Finally HMSC were used to develop methods to direct stem cell differentiation by altering physical topography of the substrate Brammer 2011 and to design surface materials to control cell adhesion properties Sommani 2010 Tsuji 2011
    https://www.bioz.com/result/bmscs/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bmscs - by Bioz Stars, 2021-05
    86/100 stars

    Images

    1) Product Images from "Effect of interleukin-1? treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells"

    Article Title: Effect of interleukin-1? treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells

    Journal: BMC Musculoskeletal Disorders

    doi: 10.1186/1471-2474-14-216

    Histological characteristics of pellets formulated from mono-cultured MCs, mono-cultured BMSCs and co-cultures of MC and BMSCs after a total of 17 days culture in defined serum-free chondrogenic media. (A-B) Safranin O and collagen II immuno-histochemical staining of representative pellets from cells derived from the same donor. Magnification lens × 20; scale bar is 100 μm.
    Figure Legend Snippet: Histological characteristics of pellets formulated from mono-cultured MCs, mono-cultured BMSCs and co-cultures of MC and BMSCs after a total of 17 days culture in defined serum-free chondrogenic media. (A-B) Safranin O and collagen II immuno-histochemical staining of representative pellets from cells derived from the same donor. Magnification lens × 20; scale bar is 100 μm.

    Techniques Used: Cell Culture, Staining, Derivative Assay

    Gene expression analysis via quantitative real time RT-PCR. mRNA gene expression analysis via SYBR Green detection was used to evaluate gene expression of: (A) aggrecan, (B) sox9, (C) collagen I ( COL1A2 ), (D) collagen II ( COL2A1 ), (E) collagen X ( COL10A1 ) (F) matrix metalloproteinase-1 (MMP-1), (G) matrix metalloproteinase −3 (MMP3), (H) matrix metalloproteinase-13 (MMP13), and (I) interleukin 1 receptor antagonist (IL1Ra) in pellets from MCs, MC:BMSCs and BMSCs after 17 days of culture in serum free chondrogenic medium containing TGF-β3, dexamethasone and ascorbate. In the last 3 days of pellet culture, pellets were either cultured in the presence (signified by +) or absence of IL-1β. Each data point represents the mean ± SEM. One-way analysis of variance (ANOVA) with Tukey’s multiple comparison post-tests was performed using SPSS version 20 and statistical significance level indicators are: *p
    Figure Legend Snippet: Gene expression analysis via quantitative real time RT-PCR. mRNA gene expression analysis via SYBR Green detection was used to evaluate gene expression of: (A) aggrecan, (B) sox9, (C) collagen I ( COL1A2 ), (D) collagen II ( COL2A1 ), (E) collagen X ( COL10A1 ) (F) matrix metalloproteinase-1 (MMP-1), (G) matrix metalloproteinase −3 (MMP3), (H) matrix metalloproteinase-13 (MMP13), and (I) interleukin 1 receptor antagonist (IL1Ra) in pellets from MCs, MC:BMSCs and BMSCs after 17 days of culture in serum free chondrogenic medium containing TGF-β3, dexamethasone and ascorbate. In the last 3 days of pellet culture, pellets were either cultured in the presence (signified by +) or absence of IL-1β. Each data point represents the mean ± SEM. One-way analysis of variance (ANOVA) with Tukey’s multiple comparison post-tests was performed using SPSS version 20 and statistical significance level indicators are: *p

    Techniques Used: Expressing, Quantitative RT-PCR, SYBR Green Assay, Cell Culture

    2) Product Images from "Influences of age-related changes in mesenchymal stem cells on macrophages during in-vitro culture"

    Article Title: Influences of age-related changes in mesenchymal stem cells on macrophages during in-vitro culture

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-017-0608-0

    Cell migration of RAW264.7 cells in Transwell systems during ( a ) or following ( b ) coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. a BMSC chemotaxis migration assay: RAW264.7 cells were placed in the inserts (upper compartments), while BMSCs were placed in the plates (lower compartments); representative images ( scale bar : 100 μm) were taken 10 h after transporting RAW264.7 cells to the Transwell systems (BMSCs were preattached in the lower plate), and statistical analysis was performed by counting the number of migrated cells in different groups. b Mφ migration assay: RAW264.7 cells following coculture (without coculture as control) were placed in the inserts (upper compartments), while no cells were placed in the plates (lower compartments); representative images ( scale bar : 100 μm) were taken 10 h after transporting RAW264.7 cells to the Transwell system, and statistical analysis was performed by counting the number of migrated cells in different groups. *p
    Figure Legend Snippet: Cell migration of RAW264.7 cells in Transwell systems during ( a ) or following ( b ) coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. a BMSC chemotaxis migration assay: RAW264.7 cells were placed in the inserts (upper compartments), while BMSCs were placed in the plates (lower compartments); representative images ( scale bar : 100 μm) were taken 10 h after transporting RAW264.7 cells to the Transwell systems (BMSCs were preattached in the lower plate), and statistical analysis was performed by counting the number of migrated cells in different groups. b Mφ migration assay: RAW264.7 cells following coculture (without coculture as control) were placed in the inserts (upper compartments), while no cells were placed in the plates (lower compartments); representative images ( scale bar : 100 μm) were taken 10 h after transporting RAW264.7 cells to the Transwell system, and statistical analysis was performed by counting the number of migrated cells in different groups. *p

    Techniques Used: Migration, Chemotaxis Assay

    Age-related differences in morphology, cell proliferation and osteogenic/adipogenic differentiation of mouse BMSCs. a Images of BMSCs showing cell morphology at passage 1 ( scale bar : 100 μm). b Growth curves of YMSCs and AMSCs (determined by CCK-8 assays). c Cell viability (EdU assays) of YMSCs and AMSCs, and statistical analysis (performed by comparing the number of EdU-positive cells in five random fields). d Potential of mouse YMSCs and AMSCs toward osteogenic differentiation analyzed via Alizarin Red S staining; images of Alizarin Red S-stained mineral nodules were acquired with an inverted microscope after 14 days of osteogenic induction ( scale bar : 100 μm) and data analysis. e Potential of mouse YMSCs and AMSCs toward adipogenic differentiation analyzed via Oil Red O staining; images of Oil Red O-stained intracellular lipid droplets were acquired with an inverted microscope after 7 days of adipogenic induction ( scale bar : 100 μm) and data analysis. Data presented as mean ± standard deviation. * p
    Figure Legend Snippet: Age-related differences in morphology, cell proliferation and osteogenic/adipogenic differentiation of mouse BMSCs. a Images of BMSCs showing cell morphology at passage 1 ( scale bar : 100 μm). b Growth curves of YMSCs and AMSCs (determined by CCK-8 assays). c Cell viability (EdU assays) of YMSCs and AMSCs, and statistical analysis (performed by comparing the number of EdU-positive cells in five random fields). d Potential of mouse YMSCs and AMSCs toward osteogenic differentiation analyzed via Alizarin Red S staining; images of Alizarin Red S-stained mineral nodules were acquired with an inverted microscope after 14 days of osteogenic induction ( scale bar : 100 μm) and data analysis. e Potential of mouse YMSCs and AMSCs toward adipogenic differentiation analyzed via Oil Red O staining; images of Oil Red O-stained intracellular lipid droplets were acquired with an inverted microscope after 7 days of adipogenic induction ( scale bar : 100 μm) and data analysis. Data presented as mean ± standard deviation. * p

    Techniques Used: CCK-8 Assay, Staining, Inverted Microscopy, Standard Deviation

    Cell surface markers (analyzed via flow cytometry) in RAW264.7 cells before or following coculture with BMSCs. a Markers CD11b and F4/80 in RAW264.7 cells before coculture. b Markers for M1 Mφs (CD86) and M2 Mφs (CD206) in RAW264.7 cells following coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. c Data analysis of cell surface markers (CD86 and CD206) following coculture. Data presented as mean ± standard deviation. *p
    Figure Legend Snippet: Cell surface markers (analyzed via flow cytometry) in RAW264.7 cells before or following coculture with BMSCs. a Markers CD11b and F4/80 in RAW264.7 cells before coculture. b Markers for M1 Mφs (CD86) and M2 Mφs (CD206) in RAW264.7 cells following coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. c Data analysis of cell surface markers (CD86 and CD206) following coculture. Data presented as mean ± standard deviation. *p

    Techniques Used: Flow Cytometry, Cytometry, Standard Deviation

    3) Product Images from "Exosomal MicroRNA-9-3p Secreted from BMSCs Downregulates ESM1 to Suppress the Development of Bladder Cancer"

    Article Title: Exosomal MicroRNA-9-3p Secreted from BMSCs Downregulates ESM1 to Suppress the Development of Bladder Cancer

    Journal: Molecular Therapy. Nucleic Acids

    doi: 10.1016/j.omtn.2019.09.023

    Schematic Diagram Showing Proposed Mechanisms by which Exosomal miR-9-3p Derived from BMSCs Inhibits the Development of Bladder Cancer Decreased miR-9-3p expression in bladder cancer reduces the inhibitory effect on ESM1. Increased ESM1 expression promotes bladder cancer cell proliferation, invasion, and metastasis, and inhibits apoptosis. miR-9-3p-containing exosomes secreted by BMSCs decrease the expression of ESM1, and hence inhibit bladder cancer cell proliferation, invasion, and metastasis, and promote apoptosis.
    Figure Legend Snippet: Schematic Diagram Showing Proposed Mechanisms by which Exosomal miR-9-3p Derived from BMSCs Inhibits the Development of Bladder Cancer Decreased miR-9-3p expression in bladder cancer reduces the inhibitory effect on ESM1. Increased ESM1 expression promotes bladder cancer cell proliferation, invasion, and metastasis, and inhibits apoptosis. miR-9-3p-containing exosomes secreted by BMSCs decrease the expression of ESM1, and hence inhibit bladder cancer cell proliferation, invasion, and metastasis, and promote apoptosis.

    Techniques Used: Derivative Assay, Expressing

    Exosome Secreted from BMSCs Affects Biological Functions of Bladder Cancer Cells (A) Representative micrographs showing exosome ultrastructure (scale bar = 100 μm). (B) Exosome size and concentration determined by NanoSight particle size analysis. (C) Representative bands showing exosomal surface marker protein expression levels of CD9, CD63, CD81, Alix, TSG101, and Calnexin. (D) Uptake of exosomes by UMUC-3 cells at different time points. Exosomes labeled with PKH26 are stained red, whereas UMUC-3 cells are stained green, and the DAPI nuclei are stained blue. A specific exosome secretion inhibitor GW4869 is added into the coculture system of BMSCs and UMUC-3 cells (scale bar = 25 μm). (E) Exosomal protein expression and concentration determined by BCA assay and NTA. (F) Western blot analysis of exosomal surface marker proteins CD9, CD63, CD81, Alix, TSG101, and Calnexin. (G) UMUC-3 cell viability measured by MTT assay. (H) UMUC-3 cell migration determined by scratch test. (I) UMUC-3 cell invasion examined by Transwell assay (scale bar = 50 μm). Measurement data are presented as mean ± SD. Independent sample t test was used for statistical analysis between the two groups. Viability of cells at different time points is analyzed by repeated-measurement ANOVA. The experiment is repeated three times. *p
    Figure Legend Snippet: Exosome Secreted from BMSCs Affects Biological Functions of Bladder Cancer Cells (A) Representative micrographs showing exosome ultrastructure (scale bar = 100 μm). (B) Exosome size and concentration determined by NanoSight particle size analysis. (C) Representative bands showing exosomal surface marker protein expression levels of CD9, CD63, CD81, Alix, TSG101, and Calnexin. (D) Uptake of exosomes by UMUC-3 cells at different time points. Exosomes labeled with PKH26 are stained red, whereas UMUC-3 cells are stained green, and the DAPI nuclei are stained blue. A specific exosome secretion inhibitor GW4869 is added into the coculture system of BMSCs and UMUC-3 cells (scale bar = 25 μm). (E) Exosomal protein expression and concentration determined by BCA assay and NTA. (F) Western blot analysis of exosomal surface marker proteins CD9, CD63, CD81, Alix, TSG101, and Calnexin. (G) UMUC-3 cell viability measured by MTT assay. (H) UMUC-3 cell migration determined by scratch test. (I) UMUC-3 cell invasion examined by Transwell assay (scale bar = 50 μm). Measurement data are presented as mean ± SD. Independent sample t test was used for statistical analysis between the two groups. Viability of cells at different time points is analyzed by repeated-measurement ANOVA. The experiment is repeated three times. *p

    Techniques Used: Concentration Assay, Particle Size Analysis, Marker, Expressing, Labeling, Staining, BIA-KA, Western Blot, MTT Assay, Migration, Transwell Assay

    4) Product Images from "In vitro differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in mouse: a proteomic analysis"

    Article Title: In vitro differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in mouse: a proteomic analysis

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Isolation, characterization and co-culture of BMSCs and EStCs. A: Flow cytometric analysis of BMSCs cell surface markers expression from primary culture. Mesenchymal stem cell surface marker CD29 expression rate was 81.22% compared to the isotype and
    Figure Legend Snippet: Isolation, characterization and co-culture of BMSCs and EStCs. A: Flow cytometric analysis of BMSCs cell surface markers expression from primary culture. Mesenchymal stem cell surface marker CD29 expression rate was 81.22% compared to the isotype and

    Techniques Used: Isolation, Co-Culture Assay, Flow Cytometry, Expressing, Marker

    5) Product Images from "Bone mesenchymal stem cells transplantation combined with mild hypothermia improves the prognosis of cerebral ischemia in rats"

    Article Title: Bone mesenchymal stem cells transplantation combined with mild hypothermia improves the prognosis of cerebral ischemia in rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0197405

    The combination therapy promoted BMSCs homing into the ischemic brain. BMSCs labeled by BrdU (red) and nuclei labeled with DAPI (blue) were observed at the lesion after MCAO. A. The homing ability was evaluated at day 1, day 5 and day 10 after MCAO. B. Quantification of migrated BMSCs. Values are expressed as mean± SE. n = 6.
    Figure Legend Snippet: The combination therapy promoted BMSCs homing into the ischemic brain. BMSCs labeled by BrdU (red) and nuclei labeled with DAPI (blue) were observed at the lesion after MCAO. A. The homing ability was evaluated at day 1, day 5 and day 10 after MCAO. B. Quantification of migrated BMSCs. Values are expressed as mean± SE. n = 6.

    Techniques Used: Labeling

    6) Product Images from "Interleukin-1β pre-treated bone marrow stromal cells alleviate neuropathic pain through CCL7-mediated inhibition of microglial activation in the spinal cord"

    Article Title: Interleukin-1β pre-treated bone marrow stromal cells alleviate neuropathic pain through CCL7-mediated inhibition of microglial activation in the spinal cord

    Journal: Scientific Reports

    doi: 10.1038/srep42260

    CXCL13 mediated the directional migration of IL-1β-BMSCs in vivo and in vitro . ( A ) Representative immunofluorescent images of the spinal cord on day 7 after SNL (day 7 after IL-1β-BMSCs injection). The middle panel and the right panel were the enlarged images of the white box parts in the left panel. Arrows in the middle and right panels are GFP-labeled IL-1β-BMSCs (GFP-BMSCs). Scale bars = 200 μm (left panel) and 50 μm (middle and right panels). ( B ) Numbers of GFP-BMSCs in ipsilateral and contralateral spinal cords on day 7 after SNL or sham operation. ( C ) Expression of CXCL13 in ipsilateral and contralateral spinal cords seven days after SNL or sham treatment. ( D ) Expression of CXCL13 in ipsilateral spinal cords on day 7 after SNL. Control or Cxcl13 shRNA lentivirus vectors were injected three days before SNL. Full-length bolts in C and D were presented in Supplementary Figure S1 . ( E ) Representative immunofluorescent images of GFP-BMSCs on the surface of an ipsilateral spinal cord. ( F ) Statistical results of the numbers GFP-BMSCs on the surface of ipsilateral spinal cords. ( G ) Representative images of transwell chemotaxis of BMSCs and IL-1β-BMSCs toward CXCL13. Scale bars = 100 μm. ( H ) Statistical results of cell migration. For ( A – F ), 32 rats in total and n = 7–8 rats/group (4 rats for immunofluorescence and 3–4 rats for western blot), and for ( H ) ten random fields at 100 × magnification were analyzed, n = 5 cultures/group. *P
    Figure Legend Snippet: CXCL13 mediated the directional migration of IL-1β-BMSCs in vivo and in vitro . ( A ) Representative immunofluorescent images of the spinal cord on day 7 after SNL (day 7 after IL-1β-BMSCs injection). The middle panel and the right panel were the enlarged images of the white box parts in the left panel. Arrows in the middle and right panels are GFP-labeled IL-1β-BMSCs (GFP-BMSCs). Scale bars = 200 μm (left panel) and 50 μm (middle and right panels). ( B ) Numbers of GFP-BMSCs in ipsilateral and contralateral spinal cords on day 7 after SNL or sham operation. ( C ) Expression of CXCL13 in ipsilateral and contralateral spinal cords seven days after SNL or sham treatment. ( D ) Expression of CXCL13 in ipsilateral spinal cords on day 7 after SNL. Control or Cxcl13 shRNA lentivirus vectors were injected three days before SNL. Full-length bolts in C and D were presented in Supplementary Figure S1 . ( E ) Representative immunofluorescent images of GFP-BMSCs on the surface of an ipsilateral spinal cord. ( F ) Statistical results of the numbers GFP-BMSCs on the surface of ipsilateral spinal cords. ( G ) Representative images of transwell chemotaxis of BMSCs and IL-1β-BMSCs toward CXCL13. Scale bars = 100 μm. ( H ) Statistical results of cell migration. For ( A – F ), 32 rats in total and n = 7–8 rats/group (4 rats for immunofluorescence and 3–4 rats for western blot), and for ( H ) ten random fields at 100 × magnification were analyzed, n = 5 cultures/group. *P

    Techniques Used: Migration, In Vivo, In Vitro, Injection, Labeling, Expressing, shRNA, Chemotaxis Assay, Immunofluorescence, Western Blot

    7) Product Images from "Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering"

    Article Title: Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering

    Journal: Polymers

    doi: 10.3390/polym8120429

    Live/Dead cell assays ( A ) and cytoskeletal arrangement determined by phalloidin/DAPI staining ( B ) of co-cultured BMSCs and chondrocytes in OC scaffolds. Bar = 300 μm; ( C ) The cytoskeletal arrangement of BMSCs and chondrocytes on a single microsphere surface in the bone and cartilage part at day 14. Bar = 150 μm.
    Figure Legend Snippet: Live/Dead cell assays ( A ) and cytoskeletal arrangement determined by phalloidin/DAPI staining ( B ) of co-cultured BMSCs and chondrocytes in OC scaffolds. Bar = 300 μm; ( C ) The cytoskeletal arrangement of BMSCs and chondrocytes on a single microsphere surface in the bone and cartilage part at day 14. Bar = 150 μm.

    Techniques Used: Staining, Cell Culture

    8) Product Images from "Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells ☆"

    Article Title: Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells ☆

    Journal: Neural Regeneration Research

    doi:

    Phosphorylated protein ERK and p38 expression in BMSCs after induction with apr-BYHWD
    Figure Legend Snippet: Phosphorylated protein ERK and p38 expression in BMSCs after induction with apr-BYHWD

    Techniques Used: Expressing

    Influence of MAPK pathway blockers on NSE expression after BMSCs induction with apr-BYHWD
    Figure Legend Snippet: Influence of MAPK pathway blockers on NSE expression after BMSCs induction with apr-BYHWD

    Techniques Used: Expressing

    Influence of MAPK pathway blockers on the nestin expression after BMSCs were induced with apr-BYHWD
    Figure Legend Snippet: Influence of MAPK pathway blockers on the nestin expression after BMSCs were induced with apr-BYHWD

    Techniques Used: Expressing

    9) Product Images from "Spindle Shaped Human Mesenchymal Stem/Stromal Cells from Amniotic Fluid Promote Neovascularization"

    Article Title: Spindle Shaped Human Mesenchymal Stem/Stromal Cells from Amniotic Fluid Promote Neovascularization

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0054747

    Analysis of angiogenic factors secreted by SS-AF-MSCs, BM-MSCs and hDFs in vitro using proteome arrays. (a-c) Representative proteome profiler arrays for (a) SS-AF-MSCs, (b) BMSCs and (c) hDFs respectively; (d) corresponding names of each molecule within the array summarized in tabular form.
    Figure Legend Snippet: Analysis of angiogenic factors secreted by SS-AF-MSCs, BM-MSCs and hDFs in vitro using proteome arrays. (a-c) Representative proteome profiler arrays for (a) SS-AF-MSCs, (b) BMSCs and (c) hDFs respectively; (d) corresponding names of each molecule within the array summarized in tabular form.

    Techniques Used: In Vitro

    SS-AF-MSCs support neovascularisation in vitro – comparing the dynamics of vessel formation in the presence of SS-AF-MSCs, hDFs or BMSCs. (a-f) Representative fields of vascular tubules after (a) 4, (b) 7, (c) 10, and (d to f) 14 days of co-culture of eGFP UCB ECFC derived cells with (a to d) SS-AF-MSCs, and pre-selected optimized batches of (e) BMSCs or (f) hDFs respectively. Scale bar = 500 µm. (g-i) Quantification of vascular tubule phenotypes at days 4, 7, 10 and 14, showing no significant difference in the number of junctions, tubules and total tubule length during the 14 days of co-culture between the 3 stromal cell types (p > 0.05 Student’s t test). Values are means±S.D. for three independent experiments.
    Figure Legend Snippet: SS-AF-MSCs support neovascularisation in vitro – comparing the dynamics of vessel formation in the presence of SS-AF-MSCs, hDFs or BMSCs. (a-f) Representative fields of vascular tubules after (a) 4, (b) 7, (c) 10, and (d to f) 14 days of co-culture of eGFP UCB ECFC derived cells with (a to d) SS-AF-MSCs, and pre-selected optimized batches of (e) BMSCs or (f) hDFs respectively. Scale bar = 500 µm. (g-i) Quantification of vascular tubule phenotypes at days 4, 7, 10 and 14, showing no significant difference in the number of junctions, tubules and total tubule length during the 14 days of co-culture between the 3 stromal cell types (p > 0.05 Student’s t test). Values are means±S.D. for three independent experiments.

    Techniques Used: In Vitro, Co-Culture Assay, Derivative Assay

    Quantitating vessel formation in in vivo studies. (a) Histological evaluation of vessels containing SS-AF-MSCs and UCB ECFC derived cells, harvested 14 days post-implantation and stained (i) with hematoxylin/eosin and for (ii-iii) human CD31 antigen (brown stain). High-power view of a vessel containing red blood cells (arrowed) from (ii) is shown in (iii). (b) Microvessel density in matrigel implants containing combined SS-AF-MSCs, BMSCs or hDFs with UCB ECFC derived cells, SS-AF-MSCs only, BMSCs only, hDFs only or UCB ECFC derived cells only. Vessel number (vessels/mm 2 ) was estimated using Image J 1.38× software. Statistical analysis was performed using Student’s t test. (c) Vessel diameter estimation in matrigel implants containing SS-AF-MSCs, BMSCs or hDFs and UCB ECFC derived cells, SS-AF-MSCs only, or UCB ECFC derived cells only using Image J 1.38× software, (*p
    Figure Legend Snippet: Quantitating vessel formation in in vivo studies. (a) Histological evaluation of vessels containing SS-AF-MSCs and UCB ECFC derived cells, harvested 14 days post-implantation and stained (i) with hematoxylin/eosin and for (ii-iii) human CD31 antigen (brown stain). High-power view of a vessel containing red blood cells (arrowed) from (ii) is shown in (iii). (b) Microvessel density in matrigel implants containing combined SS-AF-MSCs, BMSCs or hDFs with UCB ECFC derived cells, SS-AF-MSCs only, BMSCs only, hDFs only or UCB ECFC derived cells only. Vessel number (vessels/mm 2 ) was estimated using Image J 1.38× software. Statistical analysis was performed using Student’s t test. (c) Vessel diameter estimation in matrigel implants containing SS-AF-MSCs, BMSCs or hDFs and UCB ECFC derived cells, SS-AF-MSCs only, or UCB ECFC derived cells only using Image J 1.38× software, (*p

    Techniques Used: In Vivo, Derivative Assay, Staining, Software

    10) Product Images from "MicroRNA-29a-3p enhances dental implant osseointegration of hyperlipidemic rats via suppressing dishevelled 2 and frizzled 4"

    Article Title: MicroRNA-29a-3p enhances dental implant osseointegration of hyperlipidemic rats via suppressing dishevelled 2 and frizzled 4

    Journal: Cell & Bioscience

    doi: 10.1186/s13578-018-0254-y

    MiR-29a-3p expression was decreased in experiment group correlated with compromised osteogenic differentiation. a FACS was performed to identify BMSCs and assess the purity of BMSCs. Immunofluorescence was carried out to show the cytoskeletal structures and the introcellular location of ALP proteins ( b ), and Runx2 proteins ( c ). d ALP staining and ALP activity assays were applied to detect osteogenic differentiation. e ARS staining was used to determine the mineralization of osteoblasts, mn: mineralized nodule. f Oil red O staining was conducted to analyze the adipogenesis of osteoblasts, drop: lipid drop. g Relative expression levels of miR-29a-3p, ALP mRNA and Runx2 mRNA in control and experiment groups. h Western blotting was used to detect ALP and Runx2 proteins in control and experiment groups. Results are represented as mean ± SD of three independent experiments. * P
    Figure Legend Snippet: MiR-29a-3p expression was decreased in experiment group correlated with compromised osteogenic differentiation. a FACS was performed to identify BMSCs and assess the purity of BMSCs. Immunofluorescence was carried out to show the cytoskeletal structures and the introcellular location of ALP proteins ( b ), and Runx2 proteins ( c ). d ALP staining and ALP activity assays were applied to detect osteogenic differentiation. e ARS staining was used to determine the mineralization of osteoblasts, mn: mineralized nodule. f Oil red O staining was conducted to analyze the adipogenesis of osteoblasts, drop: lipid drop. g Relative expression levels of miR-29a-3p, ALP mRNA and Runx2 mRNA in control and experiment groups. h Western blotting was used to detect ALP and Runx2 proteins in control and experiment groups. Results are represented as mean ± SD of three independent experiments. * P

    Techniques Used: Expressing, FACS, Immunofluorescence, ALP Assay, Staining, Activity Assay, Western Blot

    11) Product Images from "Comparing the Osteogenic Potentials and Bone Regeneration Capacities of Bone Marrow and Dental Pulp Mesenchymal Stem Cells in a Rabbit Calvarial Bone Defect Model"

    Article Title: Comparing the Osteogenic Potentials and Bone Regeneration Capacities of Bone Marrow and Dental Pulp Mesenchymal Stem Cells in a Rabbit Calvarial Bone Defect Model

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20205015

    ( a ) Images of BMSCs and DPSCs at passage 3 after 3 days of culture. Images of BMSCs and DPSCs at × 10 and × 40 magnification revealed that both cell types exhibited a healthy fibroblastic morphology typical of MSCs. Scale bars = 200 μm. ( b ) Proliferation rates of BMSCs and DPSCs. A similar proliferation rate and tendency was found for the two cell types.
    Figure Legend Snippet: ( a ) Images of BMSCs and DPSCs at passage 3 after 3 days of culture. Images of BMSCs and DPSCs at × 10 and × 40 magnification revealed that both cell types exhibited a healthy fibroblastic morphology typical of MSCs. Scale bars = 200 μm. ( b ) Proliferation rates of BMSCs and DPSCs. A similar proliferation rate and tendency was found for the two cell types.

    Techniques Used:

    Images showing in vitro trilineage differentiation of cultured BMSCs and DPSCs. The trilineage differentiation capacities of the BMSCs and DPSCs were confirmed by alizarin red for extracellular calcium, safranin O for extracellular cartilage, and oil red O for intracellular lipid accumulation. Scale bars = 100 μm.
    Figure Legend Snippet: Images showing in vitro trilineage differentiation of cultured BMSCs and DPSCs. The trilineage differentiation capacities of the BMSCs and DPSCs were confirmed by alizarin red for extracellular calcium, safranin O for extracellular cartilage, and oil red O for intracellular lipid accumulation. Scale bars = 100 μm.

    Techniques Used: In Vitro, Cell Culture

    Comparison of osteogenic differentiation capacities of BMSCs and DPSCs. ( a ) Mineral depositions of BMSCs and DPSCs were detected by alizarin red staining following 7, 14, and 21 days of osteogenic induction. ( b ) The BMSCs showed a higher osteogenic differentiation ability than the DPSCs with statistically significant differences at different time points (** p
    Figure Legend Snippet: Comparison of osteogenic differentiation capacities of BMSCs and DPSCs. ( a ) Mineral depositions of BMSCs and DPSCs were detected by alizarin red staining following 7, 14, and 21 days of osteogenic induction. ( b ) The BMSCs showed a higher osteogenic differentiation ability than the DPSCs with statistically significant differences at different time points (** p

    Techniques Used: Staining

    Comparison of ALP (alkaline phosphatase) activity and osteogenesis-related gene expression of BMSCs and DPSCs. ( a ) The expression of ALP activity of the BMSCs and DPSCs in differentiation media and regular culture medium was evaluated after 1, 2, 3, 4, 5 and 6 days of culture. ( b ) The relative osteogenic lineage gene expression levels (ALP, RUNX2 (runt related transcription factor 2), and Osteocalcin) of the BMSCs and DPSCs were assessed by qRT-PCR (* p
    Figure Legend Snippet: Comparison of ALP (alkaline phosphatase) activity and osteogenesis-related gene expression of BMSCs and DPSCs. ( a ) The expression of ALP activity of the BMSCs and DPSCs in differentiation media and regular culture medium was evaluated after 1, 2, 3, 4, 5 and 6 days of culture. ( b ) The relative osteogenic lineage gene expression levels (ALP, RUNX2 (runt related transcription factor 2), and Osteocalcin) of the BMSCs and DPSCs were assessed by qRT-PCR (* p

    Techniques Used: ALP Assay, Activity Assay, Expressing, Quantitative RT-PCR

    Characterization of surface marker profiles for BMSCs and DPSCs. The flow cytometry results revealed that the BMSCs and DPSCs were positive for CD29, CD90, CD105, and CD146, but negative for CD34 and CD45.
    Figure Legend Snippet: Characterization of surface marker profiles for BMSCs and DPSCs. The flow cytometry results revealed that the BMSCs and DPSCs were positive for CD29, CD90, CD105, and CD146, but negative for CD34 and CD45.

    Techniques Used: Marker, Flow Cytometry, Cytometry

    Rabbit calvarial bone defects and microscopic analysis. ( a ) Scanning electron microscopy (SEM) images of BMSCs and DPSCs cultured on Bio-Oss scaffolds. ( b ) Rabbit calvarial bone defects. ( c ) Local transplantation of MSCs combined with Bio-Oss scaffolds. ( d ) Macroscopic view of regenerative areas after 3 weeks of healing. ( e ) Macroscopic view of regenerative areas after 6 weeks of healing.
    Figure Legend Snippet: Rabbit calvarial bone defects and microscopic analysis. ( a ) Scanning electron microscopy (SEM) images of BMSCs and DPSCs cultured on Bio-Oss scaffolds. ( b ) Rabbit calvarial bone defects. ( c ) Local transplantation of MSCs combined with Bio-Oss scaffolds. ( d ) Macroscopic view of regenerative areas after 3 weeks of healing. ( e ) Macroscopic view of regenerative areas after 6 weeks of healing.

    Techniques Used: Electron Microscopy, Cell Culture, Transplantation Assay

    12) Product Images from "Population doubling level-dependent change of secreted glycosaminoglycan in equine bone marrow-derived mesenchymal stem cells"

    Article Title: Population doubling level-dependent change of secreted glycosaminoglycan in equine bone marrow-derived mesenchymal stem cells

    Journal: Journal of Equine Science

    doi: 10.1294/jes.26.73

    Expression of CD44 (a), CD73 (b), CD90 (c), CD105 (d) and CD146 (e) in BMSCs at PDL-2 or PDL-12 cultured with the growth medium alone (none) or hyaluronan (HA)-supplemented medium. The values are expressed relative to the value of BMSCs at PDL-2 as the mean ± SD of three independent experiments and significant differences between the values of the respective experimental groups and PDL-2 are indicated ( *P
    Figure Legend Snippet: Expression of CD44 (a), CD73 (b), CD90 (c), CD105 (d) and CD146 (e) in BMSCs at PDL-2 or PDL-12 cultured with the growth medium alone (none) or hyaluronan (HA)-supplemented medium. The values are expressed relative to the value of BMSCs at PDL-2 as the mean ± SD of three independent experiments and significant differences between the values of the respective experimental groups and PDL-2 are indicated ( *P

    Techniques Used: Expressing, Cell Culture

    Expression of osteocalcin (a), peroxisome proliferator-activated receptor-γ (PPARγ, b) and type II collage a1 chain (Col2a1) in BMSCs of PDL-2 or PDL-12 cultured with the growth medium alone (none) or hyaluronan (HA)-supplemented medium. The values are expressed as the mean ± SD of three independent experiments and significant differences between the values of the respective experimental groups and PDL-2 are indicated ( *P
    Figure Legend Snippet: Expression of osteocalcin (a), peroxisome proliferator-activated receptor-γ (PPARγ, b) and type II collage a1 chain (Col2a1) in BMSCs of PDL-2 or PDL-12 cultured with the growth medium alone (none) or hyaluronan (HA)-supplemented medium. The values are expressed as the mean ± SD of three independent experiments and significant differences between the values of the respective experimental groups and PDL-2 are indicated ( *P

    Techniques Used: Expressing, Cell Culture

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    Article Snippet: To induce bone marrow adipocyte differentiation, mBMSCs were treated with adipogenic cocktail (30% StemXVivo Adipogenic Supplement, 1μM insulin, 2μM Rosiglitazone) for 8–10 days as previously described ( ).

    Article Title: Yellow adipocytes comprise a new adipocyte sub-type present in human bone marrow
    Article Snippet: The BM-AT that share the same macroscopic aspects compared to SC-AT was dissected from area rich in hematopoietic cells (red marrow).

    Isolation:

    Article Title: Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat
    Article Snippet: .. MSC Preparation and Characterization Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [ , ]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. .. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.

    Article Title: Persistence of Coxiella burnetii, the Agent of Q Fever, in Murine Adipose Tissue
    Article Snippet: .. Second, visceral fat depots from BALB/c mice were sampled and stromal vascular cells containing pre-adipocytes cells were isolated in 10% collagenase (Sigma-Aldrich) for 1 hour at 37°C, as previously described . ..

    Derivative Assay:

    Article Title: Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat
    Article Snippet: .. MSC Preparation and Characterization Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [ , ]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. .. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.

    Flow Cytometry:

    Article Title: Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat
    Article Snippet: .. MSC Preparation and Characterization Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [ , ]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. .. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.

    Cytometry:

    Article Title: Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat
    Article Snippet: .. MSC Preparation and Characterization Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [ , ]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. .. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.

    Migration:

    Article Title: Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat
    Article Snippet: .. MSC Preparation and Characterization Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [ , ]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. .. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.

    Chemotaxis Assay:

    Article Title: Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat
    Article Snippet: .. MSC Preparation and Characterization Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [ , ]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. .. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.

    Mouse Assay:

    Article Title: Immunosuppression in Experimental Chagas Disease Is Mediated by an Alteration of Bone Marrow Stromal Cell Function During the Acute Phase of Infection
    Article Snippet: After 3 days, non-adherent cells were collected, stained and analyzed by flow cytometry. .. To examine the influence of different stimuli on cellular stress, stromal cells of naïve mice were prepared and incubated for 4 days with serum of naïve or 14 days infected C57BL/6 wild-type mice (diluted 1:6), L-arginine (Sigma Aldrich), the arginase inhibitor N-hydroxy-L-arginine (NhLA) (Sigma Aldrich), and IL-4 (Peprotech, Hamburg, Germany). .. Subsequently, a cellular stress assay was performed (WST-1; Roche) following the manufacturer's instructions ( ).

    Article Title: Persistence of Coxiella burnetii, the Agent of Q Fever, in Murine Adipose Tissue
    Article Snippet: .. Second, visceral fat depots from BALB/c mice were sampled and stromal vascular cells containing pre-adipocytes cells were isolated in 10% collagenase (Sigma-Aldrich) for 1 hour at 37°C, as previously described . ..

    Incubation:

    Article Title: Immunosuppression in Experimental Chagas Disease Is Mediated by an Alteration of Bone Marrow Stromal Cell Function During the Acute Phase of Infection
    Article Snippet: After 3 days, non-adherent cells were collected, stained and analyzed by flow cytometry. .. To examine the influence of different stimuli on cellular stress, stromal cells of naïve mice were prepared and incubated for 4 days with serum of naïve or 14 days infected C57BL/6 wild-type mice (diluted 1:6), L-arginine (Sigma Aldrich), the arginase inhibitor N-hydroxy-L-arginine (NhLA) (Sigma Aldrich), and IL-4 (Peprotech, Hamburg, Germany). .. Subsequently, a cellular stress assay was performed (WST-1; Roche) following the manufacturer's instructions ( ).

    Infection:

    Article Title: Immunosuppression in Experimental Chagas Disease Is Mediated by an Alteration of Bone Marrow Stromal Cell Function During the Acute Phase of Infection
    Article Snippet: After 3 days, non-adherent cells were collected, stained and analyzed by flow cytometry. .. To examine the influence of different stimuli on cellular stress, stromal cells of naïve mice were prepared and incubated for 4 days with serum of naïve or 14 days infected C57BL/6 wild-type mice (diluted 1:6), L-arginine (Sigma Aldrich), the arginase inhibitor N-hydroxy-L-arginine (NhLA) (Sigma Aldrich), and IL-4 (Peprotech, Hamburg, Germany). .. Subsequently, a cellular stress assay was performed (WST-1; Roche) following the manufacturer's instructions ( ).

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    Millipore differentiation culture time points bmscs scaffold constructs
    Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + <t>BMSCs–scaffold</t> group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage <t>ECM</t> component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P
    Differentiation Culture Time Points Bmscs Scaffold Constructs, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/differentiation culture time points bmscs scaffold constructs/product/Millipore
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    Millipore bmscs
    Profound hypoxia altered metabolic profiling in BM. a Pathway analysis of 49 metabolites identified as being present in the Cy group but not in the <t>NCy</t> group. x Axis represents the pathway impact, and y axis represents the pathway enrichment. Larger sizes and darker colours represent increased pathway enrichment and higher pathway impact values, respectively. b d -galactose concentrations in the peripheral blood ( n = 22 per group) and bone marrow ( n = 5 per group) of patients were analysed. c ROS levels of <t>BMSCs</t> from the NCy and Cy groups were analysed. The data shown are the mean ± SD from three independent experiments, and statistical significance was analysed using Student’s t -test (** P
    Bmscs, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    91/100 stars
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    Image Search Results


    Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + BMSCs–scaffold group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage ECM component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P

    Journal: Stem Cell Research & Therapy

    Article Title: Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

    doi: 10.1186/s13287-020-01845-x

    Figure Lengend Snippet: Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + BMSCs–scaffold group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage ECM component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P

    Article Snippet: Assessment of ECM accumulation at different differentiation culture time points BMSCs–scaffold constructs cultured for 7, 14, 21, and 28 days were digested in 125 μg/mL papain solution (Sigma) at 55 °C overnight and then centrifuged at 10,000g for 10 min; the supernatant was collected for glycosaminoglycan (GAG) and DNA determination.

    Techniques: Immunohistochemistry, Staining

    Profound hypoxia altered metabolic profiling in BM. a Pathway analysis of 49 metabolites identified as being present in the Cy group but not in the NCy group. x Axis represents the pathway impact, and y axis represents the pathway enrichment. Larger sizes and darker colours represent increased pathway enrichment and higher pathway impact values, respectively. b d -galactose concentrations in the peripheral blood ( n = 22 per group) and bone marrow ( n = 5 per group) of patients were analysed. c ROS levels of BMSCs from the NCy and Cy groups were analysed. The data shown are the mean ± SD from three independent experiments, and statistical significance was analysed using Student’s t -test (** P

    Journal: Nature Communications

    Article Title: Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota

    doi: 10.1038/s41467-018-04453-9

    Figure Lengend Snippet: Profound hypoxia altered metabolic profiling in BM. a Pathway analysis of 49 metabolites identified as being present in the Cy group but not in the NCy group. x Axis represents the pathway impact, and y axis represents the pathway enrichment. Larger sizes and darker colours represent increased pathway enrichment and higher pathway impact values, respectively. b d -galactose concentrations in the peripheral blood ( n = 22 per group) and bone marrow ( n = 5 per group) of patients were analysed. c ROS levels of BMSCs from the NCy and Cy groups were analysed. The data shown are the mean ± SD from three independent experiments, and statistical significance was analysed using Student’s t -test (** P

    Article Snippet: To evaluate the effect of d -galactose on BMSCs, BMSCs from children in the NCy group were cultured in an incubator with low (4%) or normal (21%) O2 , and the cells were exposed to normal (50 μg/ml) or high (90 μg/ml) concentrations of d -galactose (Sigma, St. Louis, MO).

    Techniques:

    The combination therapy promoted BMSCs homing into the ischemic brain. BMSCs labeled by BrdU (red) and nuclei labeled with DAPI (blue) were observed at the lesion after MCAO. A. The homing ability was evaluated at day 1, day 5 and day 10 after MCAO. B. Quantification of migrated BMSCs. Values are expressed as mean± SE. n = 6.

    Journal: PLoS ONE

    Article Title: Bone mesenchymal stem cells transplantation combined with mild hypothermia improves the prognosis of cerebral ischemia in rats

    doi: 10.1371/journal.pone.0197405

    Figure Lengend Snippet: The combination therapy promoted BMSCs homing into the ischemic brain. BMSCs labeled by BrdU (red) and nuclei labeled with DAPI (blue) were observed at the lesion after MCAO. A. The homing ability was evaluated at day 1, day 5 and day 10 after MCAO. B. Quantification of migrated BMSCs. Values are expressed as mean± SE. n = 6.

    Article Snippet: After identification, BMSCs were incubated with 10 μmol/L BrdU (Sigma Aldrich) for 48 h before transplantation.

    Techniques: Labeling