blood mononuclear cells pbmcs (Axis-Shield Diagnostics)

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Axis-Shield Diagnostics blood mononuclear cells pbmcs

Expression of CD69 and PD-1 and apoptosis of <t>MAIT</t> cells after stimulation with IL-12 and IL-18. <t>PBMCs</t> (1 × 10 6 /well) were incubated for 24 hours in the presence of IL-12 (50 ng/mL) and IL-18 (50 ng/mL), and then stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ), and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 6 HCs. Values are expressed as the mean ± SEM. *p

Blood Mononuclear Cells Pbmcs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blood mononuclear cells pbmcs - by Bioz Stars, 2021-03

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1) Product Images from "Activation, Impaired Tumor Necrosis Factor-α Production, and Deficiency of Circulating Mucosal-Associated Invariant T Cells in Patients with Scrub Typhus"

Article Title: Activation, Impaired Tumor Necrosis Factor-α Production, and Deficiency of Circulating Mucosal-Associated Invariant T Cells in Patients with Scrub Typhus

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0004832

Expression of CD69 and PD-1 and apoptosis of MAIT cells after stimulation with IL-12 and IL-18. PBMCs (1 × 10 6 /well) were incubated for 24 hours in the presence of IL-12 (50 ng/mL) and IL-18 (50 ng/mL), and then stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ), and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 6 HCs. Values are expressed as the mean ± SEM. *p

Figure Legend Snippet: Expression of CD69 and PD-1 and apoptosis of MAIT cells after stimulation with IL-12 and IL-18. PBMCs (1 × 10 6 /well) were incubated for 24 hours in the presence of IL-12 (50 ng/mL) and IL-18 (50 ng/mL), and then stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ), and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 6 HCs. Values are expressed as the mean ± SEM. *p

Techniques Used: Expressing, Incubation, Staining, Flow Cytometry, Cytometry

Expression of IFN-γ, IL-17 and TNF-α in MAIT cells from scrub typhus patients. Freshly isolated PBMCs (1 × 10 6 /well) were incubated for 4 hours in the presence of PMA (100 ng/ml) and IM (1 μM). Panel A: Representative IFN-γ, IL-17 and TNF-α expression in the MAIT cell population were determined by intracellular flow cytometry after stimulation with PMA and IM. Data in panel B were obtained from 14 HCs and 23 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p

Figure Legend Snippet: Expression of IFN-γ, IL-17 and TNF-α in MAIT cells from scrub typhus patients. Freshly isolated PBMCs (1 × 10 6 /well) were incubated for 4 hours in the presence of PMA (100 ng/ml) and IM (1 μM). Panel A: Representative IFN-γ, IL-17 and TNF-α expression in the MAIT cell population were determined by intracellular flow cytometry after stimulation with PMA and IM. Data in panel B were obtained from 14 HCs and 23 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p

Techniques Used: Expressing, Isolation, Incubation, Flow Cytometry, Cytometry

Expression of CD69 and PD-1 and apoptosis of MAIT cells from scrub typhus patients. Freshly isolated PBMCs were stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies, and then analyzed by flow cytometry. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ) and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 14 HCs and 17 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p

Figure Legend Snippet: Expression of CD69 and PD-1 and apoptosis of MAIT cells from scrub typhus patients. Freshly isolated PBMCs were stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies, and then analyzed by flow cytometry. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ) and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 14 HCs and 17 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p

Techniques Used: Expressing, Isolation, Staining, Flow Cytometry, Cytometry

Decreased circulating MAIT cell numbers in the peripheral blood of scrub typhus patients. Freshly isolated PBMCs from 53 HCs and 38 patients with scrub typhus were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs, and then analyzed by flow cytometry. Percentages of MAIT cells were calculated using a αβ T cell gate. Panel A : Representative MAIT cell percentages as determined by flow cytometry. Panel B : MAIT cell percentages among peripheral blood αβ T cells. Panel C : Absolute MAIT cell numbers (per microliter of blood). Symbols represent individual subjects and horizontal lines are median values. *p

Figure Legend Snippet: Decreased circulating MAIT cell numbers in the peripheral blood of scrub typhus patients. Freshly isolated PBMCs from 53 HCs and 38 patients with scrub typhus were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs, and then analyzed by flow cytometry. Percentages of MAIT cells were calculated using a αβ T cell gate. Panel A : Representative MAIT cell percentages as determined by flow cytometry. Panel B : MAIT cell percentages among peripheral blood αβ T cells. Panel C : Absolute MAIT cell numbers (per microliter of blood). Symbols represent individual subjects and horizontal lines are median values. *p

Techniques Used: Isolation, Staining, Flow Cytometry, Cytometry

2) Product Images from "The interleukin-27 -964A > G polymorphism enhances sepsis-induced inflammatory responses and confers susceptibility to the development of sepsis"

Article Title: The interleukin-27 -964A > G polymorphism enhances sepsis-induced inflammatory responses and confers susceptibility to the development of sepsis

Journal: Critical Care

doi: 10.1186/s13054-018-2180-0

The IL-27 rs153109 A > G polymorphism enhanced pro-inflammatory cytokine release of the human peripheral blood mononuclear cells (PBMCs). The PBMCs of 50 healthy volunteers were incubated in vitro and stimulated with 500 ng/mL lipolysaccharide (LPS) for 8 h. Then the supernatant concentration of TNF-α, IL-6 and IL-1β in groups with different rs153109 genotypes were measured ( a - f ). The horizontal line indicates the mean expression level within each genotype group. The error bar represents standard error of the mean

Figure Legend Snippet: The IL-27 rs153109 A > G polymorphism enhanced pro-inflammatory cytokine release of the human peripheral blood mononuclear cells (PBMCs). The PBMCs of 50 healthy volunteers were incubated in vitro and stimulated with 500 ng/mL lipolysaccharide (LPS) for 8 h. Then the supernatant concentration of TNF-α, IL-6 and IL-1β in groups with different rs153109 genotypes were measured ( a - f ). The horizontal line indicates the mean expression level within each genotype group. The error bar represents standard error of the mean

Techniques Used: Incubation, In Vitro, Concentration Assay, Expressing

The IL-27 expressions in the peripheral blood mononuclear cells (PBMCs) isolated from 55 healthy volunteers. The IL-27 mRNA expression of PBMCs from 55 healthy individuals under lipopolysaccharide (LPS) stimulation (500 ng/mL) in groups with different rs153109 genotypes ( a , b ); the supernatant concentration of IL-27 under LPS stimulation in groups with different rs153109 genotypes ( c , d ). The horizontal line indicates the mean expression level in each genotype group. The error bar represents standard error of the mean

Figure Legend Snippet: The IL-27 expressions in the peripheral blood mononuclear cells (PBMCs) isolated from 55 healthy volunteers. The IL-27 mRNA expression of PBMCs from 55 healthy individuals under lipopolysaccharide (LPS) stimulation (500 ng/mL) in groups with different rs153109 genotypes ( a , b ); the supernatant concentration of IL-27 under LPS stimulation in groups with different rs153109 genotypes ( c , d ). The horizontal line indicates the mean expression level in each genotype group. The error bar represents standard error of the mean

Techniques Used: Isolation, Expressing, Concentration Assay

3) Product Images from "Interleukin-1 receptor antagonist expression is inversely associated with outcomes of hepatitis B-related acute-on-chronic liver failure"

Article Title: Interleukin-1 receptor antagonist expression is inversely associated with outcomes of hepatitis B-related acute-on-chronic liver failure

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2017.4361

Inhibition of cytokine secretion in LPS-stimulated PBMCs by rhIL-1ra. LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.

Figure Legend Snippet: Inhibition of cytokine secretion in LPS-stimulated PBMCs by rhIL-1ra. LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.

Techniques Used: Inhibition, Recombinant

Isolation:

Article Title: Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo
Article Snippet: Histone H4-derived synthetic peptides were incubated with heparinized blood at 37°C on rotation for 12 h. After centrifugation, CXCL10 was measured in the plasma using Human CXCL10/IP-10 Quantikine ELISA kit from R & D Systems (Minneapolis, MN, USA). .. Cytokine production in peripheral blood mononuclear cells (PBMCs) PBMCs were isolated from heparinized human blood using Polymorphprep (Axis-Shield, Dundee, Scotland) according to manufacturer’s protocols. ..

Article Title: Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication
Article Snippet: .. Isolation of peripheral blood mononuclear cells (PBMCs) was accomplished by using gradient centrifugation with a Lymphoprep™ (Axis-Shield, Oslo, NO). ..

Article Title: Spontaneous CD4+ and CD8+ T‐cell responses directed against cancer testis antigens are present in the peripheral blood of testicular cancer patients
Article Snippet: .. Isolation of peripheral blood mononuclear cells (PBMCs) PBMCs were isolated from heparinized blood by density gradient centrifugation over Lymphoprep (Axis‐Shield) within 4 h of collection. .. PBMCs were either assayed fresh (for dextramer analysis) or cryopreserved in liquid nitrogen (for memory phenotyping and ELISPOT analysis) in media containing 90% FCS and 10% DMSO.

Article Title: The number of circulating monocytes as biomarkers of the clinical response to methotrexate in untreated patients with rheumatoid arthritis
Article Snippet: .. Isolation of peripheral blood mononuclear cells Peripheral blood mononuclear cells (PBMC) were separated out by Ficoll-Hypaque (Lymphoprep™, Axis-Shield, Oslo, Norway) gradient centrifugation [ ]. ..

Article Title: Monocyte populations as markers of response to adalimumab plus MTX in rheumatoid arthritis
Article Snippet: .. Isolation of peripheral blood mononuclear cells Peripheral blood mononuclear cells (PBMC) were separated out by Ficoll-Hypaque (Lymphoprep™, Axis-Shield, Oslo, Norway) gradient centrifugation [ ]. ..

Gradient Centrifugation:

Article Title: Apoptosis and Mobilization of Lymphocytes to Cardiac Tissue Is Associated with Myocardial Infarction in a Reperfused Porcine Model and Infarct Size in Post-PCI Patients
Article Snippet: Total leukocyte cell count was determined using an automated blood cell counter. .. The peripheral blood mononuclear cells (PBMCs) were obtained using a density gradient centrifugation with Lymphoprep© (Axis-Shield, Norway). .. Following isolation, the PBMCs were frozen in freezing medium (10% DMSO and 90% fetal bovine serum) and stored at −80°C.

Article Title: Enhanced IgG4 production by follicular helper 2 T cells and the involvement of follicular helper 1 T cells in the pathogenesis of IgG4-related disease
Article Snippet: Proportions of lymphocyte subsets were determined by the combination of surface marker staining, with exclusion of doublets by forward and side scatter. .. Sorting of Tfh cell subsets and naïve B cells from peripheral blood mononuclear cells (PBMCs) PBMCs were separated by gradient centrifugation with a Lymphoprep (Axis-Shield; Oslo, Norway) according to the manufacturer’s instructions. .. CD4+ T cells and CD19+ B cells were enriched from PBMCs using a CD4+ T cell isolation kit and CD19+ B cell isolation kit, respectively (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s instructions.