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Axis-Shield Diagnostics blood mononuclear cells pbmcs
Expression of CD69 and PD-1 and apoptosis of <t>MAIT</t> cells after stimulation with IL-12 and IL-18. <t>PBMCs</t> (1 × 10 6 /well) were incubated for 24 hours in the presence of IL-12 (50 ng/mL) and IL-18 (50 ng/mL), and then stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ), and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 6 HCs. Values are expressed as the mean ± SEM. *p
Blood Mononuclear Cells Pbmcs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Activation, Impaired Tumor Necrosis Factor-α Production, and Deficiency of Circulating Mucosal-Associated Invariant T Cells in Patients with Scrub Typhus"

Article Title: Activation, Impaired Tumor Necrosis Factor-α Production, and Deficiency of Circulating Mucosal-Associated Invariant T Cells in Patients with Scrub Typhus

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0004832

Expression of CD69 and PD-1 and apoptosis of MAIT cells after stimulation with IL-12 and IL-18. PBMCs (1 × 10 6 /well) were incubated for 24 hours in the presence of IL-12 (50 ng/mL) and IL-18 (50 ng/mL), and then stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ), and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 6 HCs. Values are expressed as the mean ± SEM. *p
Figure Legend Snippet: Expression of CD69 and PD-1 and apoptosis of MAIT cells after stimulation with IL-12 and IL-18. PBMCs (1 × 10 6 /well) were incubated for 24 hours in the presence of IL-12 (50 ng/mL) and IL-18 (50 ng/mL), and then stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ), and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 6 HCs. Values are expressed as the mean ± SEM. *p

Techniques Used: Expressing, Incubation, Staining, Flow Cytometry, Cytometry

Expression of IFN-γ, IL-17 and TNF-α in MAIT cells from scrub typhus patients. Freshly isolated PBMCs (1 × 10 6 /well) were incubated for 4 hours in the presence of PMA (100 ng/ml) and IM (1 μM). Panel A: Representative IFN-γ, IL-17 and TNF-α expression in the MAIT cell population were determined by intracellular flow cytometry after stimulation with PMA and IM. Data in panel B were obtained from 14 HCs and 23 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p
Figure Legend Snippet: Expression of IFN-γ, IL-17 and TNF-α in MAIT cells from scrub typhus patients. Freshly isolated PBMCs (1 × 10 6 /well) were incubated for 4 hours in the presence of PMA (100 ng/ml) and IM (1 μM). Panel A: Representative IFN-γ, IL-17 and TNF-α expression in the MAIT cell population were determined by intracellular flow cytometry after stimulation with PMA and IM. Data in panel B were obtained from 14 HCs and 23 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p

Techniques Used: Expressing, Isolation, Incubation, Flow Cytometry, Cytometry

Expression of CD69 and PD-1 and apoptosis of MAIT cells from scrub typhus patients. Freshly isolated PBMCs were stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies, and then analyzed by flow cytometry. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ) and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 14 HCs and 17 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p
Figure Legend Snippet: Expression of CD69 and PD-1 and apoptosis of MAIT cells from scrub typhus patients. Freshly isolated PBMCs were stained with FITC-conjugated anti-CD3, FITC-conjugated annexin V, APC-conjugated anti-TCR Vα7.2, PE-conjugated anti-CD3, PE-conjugated anti-CD69, PE-conjugated anti-PD-1 and PE-Cy5-conjugated anti-CD161 monoclonal antibodies, and then analyzed by flow cytometry. Percentages of CD69-expressing cells ( panel A ), annexin V-positive cells ( panel C ) and PD-1-expressing cells ( panel E ) among MAIT cells were determined by flow cytometry. Data in panels B, D and F were obtained from 14 HCs and 17 patients with scrub typhus. Symbols represent individual subjects and horizontal lines are median values. *p

Techniques Used: Expressing, Isolation, Staining, Flow Cytometry, Cytometry

Decreased circulating MAIT cell numbers in the peripheral blood of scrub typhus patients. Freshly isolated PBMCs from 53 HCs and 38 patients with scrub typhus were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs, and then analyzed by flow cytometry. Percentages of MAIT cells were calculated using a αβ T cell gate. Panel A : Representative MAIT cell percentages as determined by flow cytometry. Panel B : MAIT cell percentages among peripheral blood αβ T cells. Panel C : Absolute MAIT cell numbers (per microliter of blood). Symbols represent individual subjects and horizontal lines are median values. *p
Figure Legend Snippet: Decreased circulating MAIT cell numbers in the peripheral blood of scrub typhus patients. Freshly isolated PBMCs from 53 HCs and 38 patients with scrub typhus were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs, and then analyzed by flow cytometry. Percentages of MAIT cells were calculated using a αβ T cell gate. Panel A : Representative MAIT cell percentages as determined by flow cytometry. Panel B : MAIT cell percentages among peripheral blood αβ T cells. Panel C : Absolute MAIT cell numbers (per microliter of blood). Symbols represent individual subjects and horizontal lines are median values. *p

Techniques Used: Isolation, Staining, Flow Cytometry, Cytometry

2) Product Images from "The interleukin-27 -964A > G polymorphism enhances sepsis-induced inflammatory responses and confers susceptibility to the development of sepsis"

Article Title: The interleukin-27 -964A > G polymorphism enhances sepsis-induced inflammatory responses and confers susceptibility to the development of sepsis

Journal: Critical Care

doi: 10.1186/s13054-018-2180-0

The IL-27 rs153109 A > G polymorphism enhanced pro-inflammatory cytokine release of the human peripheral blood mononuclear cells (PBMCs). The PBMCs of 50 healthy volunteers were incubated in vitro and stimulated with 500 ng/mL lipolysaccharide (LPS) for 8 h. Then the supernatant concentration of TNF-α, IL-6 and IL-1β in groups with different rs153109 genotypes were measured ( a - f ). The horizontal line indicates the mean expression level within each genotype group. The error bar represents standard error of the mean
Figure Legend Snippet: The IL-27 rs153109 A > G polymorphism enhanced pro-inflammatory cytokine release of the human peripheral blood mononuclear cells (PBMCs). The PBMCs of 50 healthy volunteers were incubated in vitro and stimulated with 500 ng/mL lipolysaccharide (LPS) for 8 h. Then the supernatant concentration of TNF-α, IL-6 and IL-1β in groups with different rs153109 genotypes were measured ( a - f ). The horizontal line indicates the mean expression level within each genotype group. The error bar represents standard error of the mean

Techniques Used: Incubation, In Vitro, Concentration Assay, Expressing

The IL-27 expressions in the peripheral blood mononuclear cells (PBMCs) isolated from 55 healthy volunteers. The IL-27 mRNA expression of PBMCs from 55 healthy individuals under lipopolysaccharide (LPS) stimulation (500 ng/mL) in groups with different rs153109 genotypes ( a , b ); the supernatant concentration of IL-27 under LPS stimulation in groups with different rs153109 genotypes ( c , d ). The horizontal line indicates the mean expression level in each genotype group. The error bar represents standard error of the mean
Figure Legend Snippet: The IL-27 expressions in the peripheral blood mononuclear cells (PBMCs) isolated from 55 healthy volunteers. The IL-27 mRNA expression of PBMCs from 55 healthy individuals under lipopolysaccharide (LPS) stimulation (500 ng/mL) in groups with different rs153109 genotypes ( a , b ); the supernatant concentration of IL-27 under LPS stimulation in groups with different rs153109 genotypes ( c , d ). The horizontal line indicates the mean expression level in each genotype group. The error bar represents standard error of the mean

Techniques Used: Isolation, Expressing, Concentration Assay

3) Product Images from "Interleukin-1 receptor antagonist expression is inversely associated with outcomes of hepatitis B-related acute-on-chronic liver failure"

Article Title: Interleukin-1 receptor antagonist expression is inversely associated with outcomes of hepatitis B-related acute-on-chronic liver failure

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2017.4361

Inhibition of cytokine secretion in LPS-stimulated PBMCs by rhIL-1ra. LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.
Figure Legend Snippet: Inhibition of cytokine secretion in LPS-stimulated PBMCs by rhIL-1ra. LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.

Techniques Used: Inhibition, Recombinant

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Article Title: Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo
Article Snippet: Histone H4-derived synthetic peptides were incubated with heparinized blood at 37°C on rotation for 12 h. After centrifugation, CXCL10 was measured in the plasma using Human CXCL10/IP-10 Quantikine ELISA kit from R & D Systems (Minneapolis, MN, USA). .. Cytokine production in peripheral blood mononuclear cells (PBMCs) PBMCs were isolated from heparinized human blood using Polymorphprep (Axis-Shield, Dundee, Scotland) according to manufacturer’s protocols. ..

Article Title: Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication
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Article Title: Spontaneous CD4+ and CD8+ T‐cell responses directed against cancer testis antigens are present in the peripheral blood of testicular cancer patients
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Article Title: The number of circulating monocytes as biomarkers of the clinical response to methotrexate in untreated patients with rheumatoid arthritis
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Article Title: Monocyte populations as markers of response to adalimumab plus MTX in rheumatoid arthritis
Article Snippet: .. Isolation of peripheral blood mononuclear cells Peripheral blood mononuclear cells (PBMC) were separated out by Ficoll-Hypaque (Lymphoprep™, Axis-Shield, Oslo, Norway) gradient centrifugation [ ]. ..

Gradient Centrifugation:

Article Title: Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication
Article Snippet: .. Isolation of peripheral blood mononuclear cells (PBMCs) was accomplished by using gradient centrifugation with a Lymphoprep™ (Axis-Shield, Oslo, NO). ..

Article Title: Spontaneous CD4+ and CD8+ T‐cell responses directed against cancer testis antigens are present in the peripheral blood of testicular cancer patients
Article Snippet: .. Isolation of peripheral blood mononuclear cells (PBMCs) PBMCs were isolated from heparinized blood by density gradient centrifugation over Lymphoprep (Axis‐Shield) within 4 h of collection. .. PBMCs were either assayed fresh (for dextramer analysis) or cryopreserved in liquid nitrogen (for memory phenotyping and ELISPOT analysis) in media containing 90% FCS and 10% DMSO.

Article Title: Apoptosis and Mobilization of Lymphocytes to Cardiac Tissue Is Associated with Myocardial Infarction in a Reperfused Porcine Model and Infarct Size in Post-PCI Patients
Article Snippet: Total leukocyte cell count was determined using an automated blood cell counter. .. The peripheral blood mononuclear cells (PBMCs) were obtained using a density gradient centrifugation with Lymphoprep© (Axis-Shield, Norway). .. Following isolation, the PBMCs were frozen in freezing medium (10% DMSO and 90% fetal bovine serum) and stored at −80°C.

Article Title: Enhanced IgG4 production by follicular helper 2 T cells and the involvement of follicular helper 1 T cells in the pathogenesis of IgG4-related disease
Article Snippet: Proportions of lymphocyte subsets were determined by the combination of surface marker staining, with exclusion of doublets by forward and side scatter. .. Sorting of Tfh cell subsets and naïve B cells from peripheral blood mononuclear cells (PBMCs) PBMCs were separated by gradient centrifugation with a Lymphoprep (Axis-Shield; Oslo, Norway) according to the manufacturer’s instructions. .. CD4+ T cells and CD19+ B cells were enriched from PBMCs using a CD4+ T cell isolation kit and CD19+ B cell isolation kit, respectively (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s instructions.

Article Title: The number of circulating monocytes as biomarkers of the clinical response to methotrexate in untreated patients with rheumatoid arthritis
Article Snippet: .. Isolation of peripheral blood mononuclear cells Peripheral blood mononuclear cells (PBMC) were separated out by Ficoll-Hypaque (Lymphoprep™, Axis-Shield, Oslo, Norway) gradient centrifugation [ ]. ..

Article Title: Monocyte populations as markers of response to adalimumab plus MTX in rheumatoid arthritis
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    Axis-Shield Diagnostics peripheral blood mononuclear cells pbmcs
    Comparison of HBV-specific cell responses in the presence or absence of HBeAg. (A) C protein-specific T-cell responses elicited in ELISPOT assay; (B) Cytokine <t>IFN-Y</t> responses in cultured supernatants of <t>PBMCs</t> stimulated by C protein. HBeAg(−): HBeAg-negative subjects; HBeAg(+): HBeAg-positive subjects; HIV+: HIV co-infected; HIV-: not co-infected with HIV.
    Peripheral Blood Mononuclear Cells Pbmcs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmcs/product/Axis-Shield Diagnostics
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Axis-Shield Diagnostics blood mononuclear cells pbmcs
    Inhibition of cytokine secretion in LPS-stimulated <t>PBMCs</t> by <t>rhIL-1ra.</t> LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.
    Blood Mononuclear Cells Pbmcs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blood mononuclear cells pbmcs/product/Axis-Shield Diagnostics
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Axis-Shield Diagnostics human peripheral blood mononuclear cells pbmcs
    Infection with Mtb HN878 triggers increased IL-22 production through a TLR2-dependent pathway Lung cells isolated from B6, Il1r-/- and Tlr2-/- mice were infected with either Mtb H37Rv (Rv) or Mtb HN878 (HN) at MOI of 0.1 for 7 days, and the protein levels of ( a ) IL-1β and ( b ) IL-22 were assessed in culture supernatants by ELISA and luminex assay, respectively. Lung cells isolated from B6 mice were treated with cell wall extracted from Mtb H37Rv (Rv) or Mtb HN878 (HN), (10μg/ml each). Protein levels of ( c ) IL-1β and ( d ) IL-22 were assessed by ELISA and luminex assay respectively. <t>PBMCs</t> were isolated from <t>ATB</t> patients and were stimulated with Mtb H37Rv (Rv) or Mtb HN878 (HN) cell wall extracts (20 μg/ml each) and the protein levels of ( e ) IL-22 were measured by luminex assays. Error bars represent means ± s.e.m. n = 3-5 for a-d. n = 17 for human ATB patients for e. ND, not detected; UI, uninfected; UN, untreated. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 by ( a,b ) one-way ANOVA, ( c,d ) Student's t test ( e ) two-way ANOVA
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/Axis-Shield Diagnostics
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
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    Comparison of HBV-specific cell responses in the presence or absence of HBeAg. (A) C protein-specific T-cell responses elicited in ELISPOT assay; (B) Cytokine IFN-Y responses in cultured supernatants of PBMCs stimulated by C protein. HBeAg(−): HBeAg-negative subjects; HBeAg(+): HBeAg-positive subjects; HIV+: HIV co-infected; HIV-: not co-infected with HIV.

    Journal: Virology Journal

    Article Title: Hepatitis B virus (HBV)-specific T-cell responses to recombinant HBV core protein in patients with normal liver function and co-infected with chronic HBV and human immunodeficiency virus 1 (HIV-1)

    doi: 10.1186/1743-422X-10-232

    Figure Lengend Snippet: Comparison of HBV-specific cell responses in the presence or absence of HBeAg. (A) C protein-specific T-cell responses elicited in ELISPOT assay; (B) Cytokine IFN-Y responses in cultured supernatants of PBMCs stimulated by C protein. HBeAg(−): HBeAg-negative subjects; HBeAg(+): HBeAg-positive subjects; HIV+: HIV co-infected; HIV-: not co-infected with HIV.

    Article Snippet: Human interferon (IFN)-γ ELISPOT assay Peripheral whole blood was obtained from all subjects and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with Ficoll Lymphoprep (Axis –Shield PoC AS, Oslo, Norway).

    Techniques: Enzyme-linked Immunospot, Cell Culture, Infection

    Lenalidomide maintenance therapy does not enhance MM-specific CD4 + T-cell responses Analysis of T-cell responses specific for the MAGE-C1 peptide SQSSPVSSFPSSTSS. ( A ) CD137 expression on CD4 + and CD8 + T cells from patient#02 at the pre LEN timepoint upon in vitro restimulation with the MAGE-C1 peptide. ( B ) PBMCs obtained at the pre LEN and the LEN timepoint were examined by IFN-γ ELISPOT to measure the T-cell response to the MAGE-C1 peptide. The bar graphs show the mean number of spots per million cells of four replicate wells and the error bars represent the standard deviation. A two-way ANOVA with Sidak’s multiple comparisons test was performed to evaluate statistical significant differences, ns: not significant, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001.

    Journal: Oncotarget

    Article Title: Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation

    doi: 10.18632/oncotarget.24944

    Figure Lengend Snippet: Lenalidomide maintenance therapy does not enhance MM-specific CD4 + T-cell responses Analysis of T-cell responses specific for the MAGE-C1 peptide SQSSPVSSFPSSTSS. ( A ) CD137 expression on CD4 + and CD8 + T cells from patient#02 at the pre LEN timepoint upon in vitro restimulation with the MAGE-C1 peptide. ( B ) PBMCs obtained at the pre LEN and the LEN timepoint were examined by IFN-γ ELISPOT to measure the T-cell response to the MAGE-C1 peptide. The bar graphs show the mean number of spots per million cells of four replicate wells and the error bars represent the standard deviation. A two-way ANOVA with Sidak’s multiple comparisons test was performed to evaluate statistical significant differences, ns: not significant, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001.

    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from leukapheresis (pre-LEN timepoint) and whole blood (LEN timepoint) samples through lymphoprep gradient-centrifugation (Axis-Shield) following the manufacturer’s instructions before being cryopreserved.

    Techniques: Expressing, In Vitro, Enzyme-linked Immunospot, Standard Deviation

    Lenalidomide maintenance therapy results in an improved CD8 + T cell functionality, which is hampered by non-CD8 + T cells present in the periphery ( A ) PBMCs obtained at the pre LEN and the LEN timepoint were examined by IFN-γ ELISPOT to measure the CD8 + T-cell response to viral recall epitopes derived for CMV, EBV and influenza (CEF) ( n = 6). The number of spot forming units (SFU) as well as the activity in each well was determined. Bar graphs show the mean number of spots per million PBMCs or the mean activity of four replicate wells and the error bars show the standard deviation. Spot numbers and activity measured upon culture in the absence of peptide were subtracted. ( B – E ) CD8 + T cells purified from PBMC samples obtained at the pre LEN and LEN timepoint were examined by IFN-γ/IL-2/TNF-α FLUOROSPOT to characterize the CD8 + T-cell response to the CEF peptide pool. (B–C) Bar graphs show the mean number of spots per million CD8 + T cells of five replicate wells and the error bars show the standard deviation. Spot numbers and activity measured upon culture in the absence of peptide were subtracted. ( D ) Raw data showing three-color FLUOROSPOT results. One representative well is shown for each patient and timepoint. Cytokine-specific spots are shown in green (IFN-γ), red (TNF-α), blue (IL-2) or overlays of these colors. ( E ) The pie charts indicate the portion of mono- and polyfunctional CEF-specific CD8 + T cells. A two-way ANOVA with Sidak’s multiple comparisons test was performed to detect differences between the pre LEN and the LEN timepoint, ns: not significant, * p

    Journal: Oncotarget

    Article Title: Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation

    doi: 10.18632/oncotarget.24944

    Figure Lengend Snippet: Lenalidomide maintenance therapy results in an improved CD8 + T cell functionality, which is hampered by non-CD8 + T cells present in the periphery ( A ) PBMCs obtained at the pre LEN and the LEN timepoint were examined by IFN-γ ELISPOT to measure the CD8 + T-cell response to viral recall epitopes derived for CMV, EBV and influenza (CEF) ( n = 6). The number of spot forming units (SFU) as well as the activity in each well was determined. Bar graphs show the mean number of spots per million PBMCs or the mean activity of four replicate wells and the error bars show the standard deviation. Spot numbers and activity measured upon culture in the absence of peptide were subtracted. ( B – E ) CD8 + T cells purified from PBMC samples obtained at the pre LEN and LEN timepoint were examined by IFN-γ/IL-2/TNF-α FLUOROSPOT to characterize the CD8 + T-cell response to the CEF peptide pool. (B–C) Bar graphs show the mean number of spots per million CD8 + T cells of five replicate wells and the error bars show the standard deviation. Spot numbers and activity measured upon culture in the absence of peptide were subtracted. ( D ) Raw data showing three-color FLUOROSPOT results. One representative well is shown for each patient and timepoint. Cytokine-specific spots are shown in green (IFN-γ), red (TNF-α), blue (IL-2) or overlays of these colors. ( E ) The pie charts indicate the portion of mono- and polyfunctional CEF-specific CD8 + T cells. A two-way ANOVA with Sidak’s multiple comparisons test was performed to detect differences between the pre LEN and the LEN timepoint, ns: not significant, * p

    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from leukapheresis (pre-LEN timepoint) and whole blood (LEN timepoint) samples through lymphoprep gradient-centrifugation (Axis-Shield) following the manufacturer’s instructions before being cryopreserved.

    Techniques: Enzyme-linked Immunospot, Derivative Assay, Activity Assay, Standard Deviation, Purification

    Inhibition of cytokine secretion in LPS-stimulated PBMCs by rhIL-1ra. LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Interleukin-1 receptor antagonist expression is inversely associated with outcomes of hepatitis B-related acute-on-chronic liver failure

    doi: 10.3892/etm.2017.4361

    Figure Lengend Snippet: Inhibition of cytokine secretion in LPS-stimulated PBMCs by rhIL-1ra. LPS, lipopolysaccharide; PBMCs, peripheral blood mononuclear cells; rh, human recombinant; IL, interleukin; ra, receptor antagonist; low, LPS + 125 ng/ml rhIL-1ra; middle, LPS + 250 ng/ml rhIL-1ra; high, LPS + 500 ng/ml rhIL-1ra; IFN, interferon; TNF, tumor necrosis factor.

    Article Snippet: Incubation of peripheral blood mononuclear cells (PBMCs) with IL-1ra in vitro PBMCs were isolated from the heparinized blood of 31 patients with ACLF via centrifugation at 800 × g at 20°C for 20 min using Ficoll Lymphoprep (Axis-Shield Diagnostics Ltd., Dundee, UK).

    Techniques: Inhibition, Recombinant

    Infection with Mtb HN878 triggers increased IL-22 production through a TLR2-dependent pathway Lung cells isolated from B6, Il1r-/- and Tlr2-/- mice were infected with either Mtb H37Rv (Rv) or Mtb HN878 (HN) at MOI of 0.1 for 7 days, and the protein levels of ( a ) IL-1β and ( b ) IL-22 were assessed in culture supernatants by ELISA and luminex assay, respectively. Lung cells isolated from B6 mice were treated with cell wall extracted from Mtb H37Rv (Rv) or Mtb HN878 (HN), (10μg/ml each). Protein levels of ( c ) IL-1β and ( d ) IL-22 were assessed by ELISA and luminex assay respectively. PBMCs were isolated from ATB patients and were stimulated with Mtb H37Rv (Rv) or Mtb HN878 (HN) cell wall extracts (20 μg/ml each) and the protein levels of ( e ) IL-22 were measured by luminex assays. Error bars represent means ± s.e.m. n = 3-5 for a-d. n = 17 for human ATB patients for e. ND, not detected; UI, uninfected; UN, untreated. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 by ( a,b ) one-way ANOVA, ( c,d ) Student's t test ( e ) two-way ANOVA

    Journal: Mucosal immunology

    Article Title: Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection

    doi: 10.1038/mi.2017.15

    Figure Lengend Snippet: Infection with Mtb HN878 triggers increased IL-22 production through a TLR2-dependent pathway Lung cells isolated from B6, Il1r-/- and Tlr2-/- mice were infected with either Mtb H37Rv (Rv) or Mtb HN878 (HN) at MOI of 0.1 for 7 days, and the protein levels of ( a ) IL-1β and ( b ) IL-22 were assessed in culture supernatants by ELISA and luminex assay, respectively. Lung cells isolated from B6 mice were treated with cell wall extracted from Mtb H37Rv (Rv) or Mtb HN878 (HN), (10μg/ml each). Protein levels of ( c ) IL-1β and ( d ) IL-22 were assessed by ELISA and luminex assay respectively. PBMCs were isolated from ATB patients and were stimulated with Mtb H37Rv (Rv) or Mtb HN878 (HN) cell wall extracts (20 μg/ml each) and the protein levels of ( e ) IL-22 were measured by luminex assays. Error bars represent means ± s.e.m. n = 3-5 for a-d. n = 17 for human ATB patients for e. ND, not detected; UI, uninfected; UN, untreated. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 by ( a,b ) one-way ANOVA, ( c,d ) Student's t test ( e ) two-way ANOVA

    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) from ATB patients were isolated by Ficoll Hypaque gradient (Lymphoprep; Axis-Shield POC AS, Oslo, Norway ).

    Techniques: Infection, Isolation, Mouse Assay, Enzyme-linked Immunosorbent Assay, Luminex