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Funakoshi blockace
Blockace, supplied by Funakoshi, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blockace/product/Funakoshi
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
blockace - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Microscopy:

Article Title: Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture
Article Snippet: After blocking with BlockAce for at least 30 min at 4 °C, the slices were incubated with the primary antibody ( ) for 60 min, rinsed with PBS, and then incubated with a secondary antibody for 30 min. Next, the sections were incubated with an avidin-conjugated biotinylated antibody (VECTASTAIN ABC kit; Funakoshi, Osaka, Japan). .. Images were obtained using a Zeiss 780 confocal microscope (Carl Zeiss, Oberkochen, Germany) and a confocal laser microscope (Olympus, Tokyo, Japan).

Avidin-Biotin Assay:

Article Title: Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture
Article Snippet: .. After blocking with BlockAce for at least 30 min at 4 °C, the slices were incubated with the primary antibody ( ) for 60 min, rinsed with PBS, and then incubated with a secondary antibody for 30 min. Next, the sections were incubated with an avidin-conjugated biotinylated antibody (VECTASTAIN ABC kit; Funakoshi, Osaka, Japan). ..

Labeling:

Article Title: Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN
Article Snippet: After blocking with BlockAce (Funakoshi, Tokyo, Japan, ; catalog no. BUF029), the membrane was incubated with horseradish peroxidase (HRP)-conjugated rBC2LCN (1 μg/ml) or goat anti-podocalyxin pAb (0.2 mg/ml; R & D Systems Inc., Minneapolis, MN, ; catalog no. AF1658) followed by HRP-conjugated donkey anti-goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA, ; catalog no. 705-035-003). .. The HRP labeling of rBC2LCN was performed using a peroxidase labeling kit (Dojindo Molecular Technologies Inc., Gaithersburg, MD, ; LK11).

Immunoprecipitation:

Article Title: Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN
Article Snippet: One microgram of the hydrophilic fractions or 5 μl of the immunoprecipitated samples described above were electrophoresed under reducing conditions on 5%–20% polyacrylamide gel (Dream Realization & Communication, Tokyo, Japan, ; catalog no. NTH-676HP). .. After blocking with BlockAce (Funakoshi, Tokyo, Japan, ; catalog no. BUF029), the membrane was incubated with horseradish peroxidase (HRP)-conjugated rBC2LCN (1 μg/ml) or goat anti-podocalyxin pAb (0.2 mg/ml; R & D Systems Inc., Minneapolis, MN, ; catalog no. AF1658) followed by HRP-conjugated donkey anti-goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA, ; catalog no. 705-035-003).

Incubation:

Article Title: Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
Article Snippet: .. After blocking with BlockAce (Cat#: BUF029, Funakoshi Co., Ltd., Tokyo, Japan), the membrane was incubated with 500-fold dilution of anti-CD9 (Clone#: Ts9, Cat#: 10626D, Thermo Fisher Scientific K.K.) and 2000-fold dilution of anti-CD63 (Clone#: 8A12, Cat#: SHI-EXO-M02, COSMO BIO Co. Ltd., Tokyo, Japan) followed by 10,000-fold dilution of peroxidase-labeled AffiniPure Goat Anti-Mouse IgG (H+L) (Cat#: 115-035-003, Jackson ImmunoResearch Inc., West Grove, PA). .. Production of high-density lectin microarray High-density lectin microarray immobilizing 96 lectins was produced as previously described , .

Article Title: Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture
Article Snippet: .. After blocking with BlockAce for at least 30 min at 4 °C, the slices were incubated with the primary antibody ( ) for 60 min, rinsed with PBS, and then incubated with a secondary antibody for 30 min. Next, the sections were incubated with an avidin-conjugated biotinylated antibody (VECTASTAIN ABC kit; Funakoshi, Osaka, Japan). ..

Article Title: Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN
Article Snippet: .. After blocking with BlockAce (Funakoshi, Tokyo, Japan, ; catalog no. BUF029), the membrane was incubated with horseradish peroxidase (HRP)-conjugated rBC2LCN (1 μg/ml) or goat anti-podocalyxin pAb (0.2 mg/ml; R & D Systems Inc., Minneapolis, MN, ; catalog no. AF1658) followed by HRP-conjugated donkey anti-goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA, ; catalog no. 705-035-003). .. Silver staining of the gel was also performed using a silver staining kit (Wako Chemical, Osaka, Japan, ; catalog no. 299-58901).

Activity Assay:

Article Title: Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture
Article Snippet: Deparaffinized sections were treated with 0.6% H2 O2 in methanol for 30 min to suppress endogenous peroxidase activity. .. After blocking with BlockAce for at least 30 min at 4 °C, the slices were incubated with the primary antibody ( ) for 60 min, rinsed with PBS, and then incubated with a secondary antibody for 30 min. Next, the sections were incubated with an avidin-conjugated biotinylated antibody (VECTASTAIN ABC kit; Funakoshi, Osaka, Japan).

Blocking Assay:

Article Title: Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
Article Snippet: .. After blocking with BlockAce (Cat#: BUF029, Funakoshi Co., Ltd., Tokyo, Japan), the membrane was incubated with 500-fold dilution of anti-CD9 (Clone#: Ts9, Cat#: 10626D, Thermo Fisher Scientific K.K.) and 2000-fold dilution of anti-CD63 (Clone#: 8A12, Cat#: SHI-EXO-M02, COSMO BIO Co. Ltd., Tokyo, Japan) followed by 10,000-fold dilution of peroxidase-labeled AffiniPure Goat Anti-Mouse IgG (H+L) (Cat#: 115-035-003, Jackson ImmunoResearch Inc., West Grove, PA). .. Production of high-density lectin microarray High-density lectin microarray immobilizing 96 lectins was produced as previously described , .

Article Title: Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture
Article Snippet: .. After blocking with BlockAce for at least 30 min at 4 °C, the slices were incubated with the primary antibody ( ) for 60 min, rinsed with PBS, and then incubated with a secondary antibody for 30 min. Next, the sections were incubated with an avidin-conjugated biotinylated antibody (VECTASTAIN ABC kit; Funakoshi, Osaka, Japan). ..

Article Title: Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN
Article Snippet: .. After blocking with BlockAce (Funakoshi, Tokyo, Japan, ; catalog no. BUF029), the membrane was incubated with horseradish peroxidase (HRP)-conjugated rBC2LCN (1 μg/ml) or goat anti-podocalyxin pAb (0.2 mg/ml; R & D Systems Inc., Minneapolis, MN, ; catalog no. AF1658) followed by HRP-conjugated donkey anti-goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA, ; catalog no. 705-035-003). .. Silver staining of the gel was also performed using a silver staining kit (Wako Chemical, Osaka, Japan, ; catalog no. 299-58901).

Mass Spectrometry:

Article Title: Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
Article Snippet: SDS PAGE and Western blotting EVs (0.2 μg) were electrophoresed under non-reducing conditions on 5–20% polyacrylamide gels (Cat#: NTH-676HP, DRC, Tokyo, Japan) and visualized using the Sliver Staining MS kit (Cat#: 293-77601, Wako Pure Chemical Industries, Ltd.) according to the manufacturer’s instructions. .. After blocking with BlockAce (Cat#: BUF029, Funakoshi Co., Ltd., Tokyo, Japan), the membrane was incubated with 500-fold dilution of anti-CD9 (Clone#: Ts9, Cat#: 10626D, Thermo Fisher Scientific K.K.) and 2000-fold dilution of anti-CD63 (Clone#: 8A12, Cat#: SHI-EXO-M02, COSMO BIO Co. Ltd., Tokyo, Japan) followed by 10,000-fold dilution of peroxidase-labeled AffiniPure Goat Anti-Mouse IgG (H+L) (Cat#: 115-035-003, Jackson ImmunoResearch Inc., West Grove, PA).

Western Blot:

Article Title: Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
Article Snippet: Paragraph title: SDS PAGE and Western blotting ... After blocking with BlockAce (Cat#: BUF029, Funakoshi Co., Ltd., Tokyo, Japan), the membrane was incubated with 500-fold dilution of anti-CD9 (Clone#: Ts9, Cat#: 10626D, Thermo Fisher Scientific K.K.) and 2000-fold dilution of anti-CD63 (Clone#: 8A12, Cat#: SHI-EXO-M02, COSMO BIO Co. Ltd., Tokyo, Japan) followed by 10,000-fold dilution of peroxidase-labeled AffiniPure Goat Anti-Mouse IgG (H+L) (Cat#: 115-035-003, Jackson ImmunoResearch Inc., West Grove, PA).

Article Title: Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN
Article Snippet: Paragraph title: Western and Lectin Blotting ... After blocking with BlockAce (Funakoshi, Tokyo, Japan, ; catalog no. BUF029), the membrane was incubated with horseradish peroxidase (HRP)-conjugated rBC2LCN (1 μg/ml) or goat anti-podocalyxin pAb (0.2 mg/ml; R & D Systems Inc., Minneapolis, MN, ; catalog no. AF1658) followed by HRP-conjugated donkey anti-goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA, ; catalog no. 705-035-003).

Staining:

Article Title: Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
Article Snippet: SDS PAGE and Western blotting EVs (0.2 μg) were electrophoresed under non-reducing conditions on 5–20% polyacrylamide gels (Cat#: NTH-676HP, DRC, Tokyo, Japan) and visualized using the Sliver Staining MS kit (Cat#: 293-77601, Wako Pure Chemical Industries, Ltd.) according to the manufacturer’s instructions. .. After blocking with BlockAce (Cat#: BUF029, Funakoshi Co., Ltd., Tokyo, Japan), the membrane was incubated with 500-fold dilution of anti-CD9 (Clone#: Ts9, Cat#: 10626D, Thermo Fisher Scientific K.K.) and 2000-fold dilution of anti-CD63 (Clone#: 8A12, Cat#: SHI-EXO-M02, COSMO BIO Co. Ltd., Tokyo, Japan) followed by 10,000-fold dilution of peroxidase-labeled AffiniPure Goat Anti-Mouse IgG (H+L) (Cat#: 115-035-003, Jackson ImmunoResearch Inc., West Grove, PA).

Silver Staining:

Article Title: Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN
Article Snippet: After blocking with BlockAce (Funakoshi, Tokyo, Japan, ; catalog no. BUF029), the membrane was incubated with horseradish peroxidase (HRP)-conjugated rBC2LCN (1 μg/ml) or goat anti-podocalyxin pAb (0.2 mg/ml; R & D Systems Inc., Minneapolis, MN, ; catalog no. AF1658) followed by HRP-conjugated donkey anti-goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA, ; catalog no. 705-035-003). .. Silver staining of the gel was also performed using a silver staining kit (Wako Chemical, Osaka, Japan, ; catalog no. 299-58901).

SDS Page:

Article Title: Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
Article Snippet: Paragraph title: SDS PAGE and Western blotting ... After blocking with BlockAce (Cat#: BUF029, Funakoshi Co., Ltd., Tokyo, Japan), the membrane was incubated with 500-fold dilution of anti-CD9 (Clone#: Ts9, Cat#: 10626D, Thermo Fisher Scientific K.K.) and 2000-fold dilution of anti-CD63 (Clone#: 8A12, Cat#: SHI-EXO-M02, COSMO BIO Co. Ltd., Tokyo, Japan) followed by 10,000-fold dilution of peroxidase-labeled AffiniPure Goat Anti-Mouse IgG (H+L) (Cat#: 115-035-003, Jackson ImmunoResearch Inc., West Grove, PA).

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    Funakoshi blockace
    Blockace, supplied by Funakoshi, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blockace/product/Funakoshi
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    blockace - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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