block it fluorescent oligo  (Thermo Fisher)


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    Name:
    Oligo dT Primer 50 µM
    Description:
    These are the same Ambion primers that are currently included in the RETROscript Kit SKU AM1710 They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript Kit
    Catalog Number:
    am5730g
    Price:
    None
    Category:
    Oligos Primers Probes Nucleotides
    Applications:
    PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR
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    Structured Review

    Thermo Fisher block it fluorescent oligo
    These are the same Ambion primers that are currently included in the RETROscript Kit SKU AM1710 They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript Kit
    https://www.bioz.com/result/block it fluorescent oligo/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    block it fluorescent oligo - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Infection:

    Article Title: Entry of Human Papillomavirus Type 16 by Actin-Dependent, Clathrin- and Lipid Raft-Independent Endocytosis
    Article Snippet: .. Infection studies and analysis by automated microscopy For siRNA mediated knockdown experiments, 3,000 cells were reverse transfected in optical bottom 96-well plates (Nunc) with the indicated amount of siRNA oligos using Lipofectamine RNAimax (Invitrogen), and infected 48 h post transfection of siRNAs. .. In case of oligos directed against clathrin heavy chain and the AP2 μ subunit, cells were transfected for a second time 24 h post initial transfection.

    Microscopy:

    Article Title: Entry of Human Papillomavirus Type 16 by Actin-Dependent, Clathrin- and Lipid Raft-Independent Endocytosis
    Article Snippet: .. Infection studies and analysis by automated microscopy For siRNA mediated knockdown experiments, 3,000 cells were reverse transfected in optical bottom 96-well plates (Nunc) with the indicated amount of siRNA oligos using Lipofectamine RNAimax (Invitrogen), and infected 48 h post transfection of siRNAs. .. In case of oligos directed against clathrin heavy chain and the AP2 μ subunit, cells were transfected for a second time 24 h post initial transfection.

    Transfection:

    Article Title: Entry of Human Papillomavirus Type 16 by Actin-Dependent, Clathrin- and Lipid Raft-Independent Endocytosis
    Article Snippet: .. Infection studies and analysis by automated microscopy For siRNA mediated knockdown experiments, 3,000 cells were reverse transfected in optical bottom 96-well plates (Nunc) with the indicated amount of siRNA oligos using Lipofectamine RNAimax (Invitrogen), and infected 48 h post transfection of siRNAs. .. In case of oligos directed against clathrin heavy chain and the AP2 μ subunit, cells were transfected for a second time 24 h post initial transfection.

    Article Title: Genomic HIV RNA Induces Innate Immune Responses through RIG-I-Dependent Sensing of Secondary-Structured RNA
    Article Snippet: .. RNA transfection RNA oligos or genomic HIV RNA was transfected into cells using Lipofectamine 2000 (Invitrogen). ..

    Article Title: miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors
    Article Snippet: .. Cell Transfection Cells were transfected with 30 mM Ambion pre-miR-411 precursor oligos (Life Technologies) using Lipofectamine 2000 (Life Technologies) following the manufacture protocol. .. Briefly, 4×103 /cm2 C2 C12 cells were plated in growth medium one day before the transfection.

    Article Title: Berberine Radiosensitizes Human Esophageal Cancer Cells by Downregulating Homologous Recombination Repair Protein RAD51
    Article Snippet: Renilla luciferase activity from a cotransfected pRL-SV40 control vector (50 ng/well) was used for normalization. .. RAD51 depletion by RNA interference RAD51 siRNA duplex 1, 2 and 3 and the negative oligo were obtained from GenePharma (Shanghai, China). siRNA duplexes or negative oligo (200 nM) were transfected into KYSE30 and KYSE450 cells using lipofectamine2000 (Invitrogen) for 24 h. RAD51 protein levels were determined by western blotting analysis and the levels of radiosensitivity were determined by clonogenic assay described above. .. Sequences for small interfering RNA for RAD51 RNAi are as follows: siRAD51-1: CGAUGUGAAGAAAUUGGAATT siRAD51-2: GACUGGAUCUAUCACAGAATT siRAD51-3: GCAGUGAUGUCCUGGAUAATT

    Western Blot:

    Article Title: Berberine Radiosensitizes Human Esophageal Cancer Cells by Downregulating Homologous Recombination Repair Protein RAD51
    Article Snippet: Renilla luciferase activity from a cotransfected pRL-SV40 control vector (50 ng/well) was used for normalization. .. RAD51 depletion by RNA interference RAD51 siRNA duplex 1, 2 and 3 and the negative oligo were obtained from GenePharma (Shanghai, China). siRNA duplexes or negative oligo (200 nM) were transfected into KYSE30 and KYSE450 cells using lipofectamine2000 (Invitrogen) for 24 h. RAD51 protein levels were determined by western blotting analysis and the levels of radiosensitivity were determined by clonogenic assay described above. .. Sequences for small interfering RNA for RAD51 RNAi are as follows: siRAD51-1: CGAUGUGAAGAAAUUGGAATT siRAD51-2: GACUGGAUCUAUCACAGAATT siRAD51-3: GCAGUGAUGUCCUGGAUAATT

    Clonogenic Assay:

    Article Title: Berberine Radiosensitizes Human Esophageal Cancer Cells by Downregulating Homologous Recombination Repair Protein RAD51
    Article Snippet: Renilla luciferase activity from a cotransfected pRL-SV40 control vector (50 ng/well) was used for normalization. .. RAD51 depletion by RNA interference RAD51 siRNA duplex 1, 2 and 3 and the negative oligo were obtained from GenePharma (Shanghai, China). siRNA duplexes or negative oligo (200 nM) were transfected into KYSE30 and KYSE450 cells using lipofectamine2000 (Invitrogen) for 24 h. RAD51 protein levels were determined by western blotting analysis and the levels of radiosensitivity were determined by clonogenic assay described above. .. Sequences for small interfering RNA for RAD51 RNAi are as follows: siRAD51-1: CGAUGUGAAGAAAUUGGAATT siRAD51-2: GACUGGAUCUAUCACAGAATT siRAD51-3: GCAGUGAUGUCCUGGAUAATT

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  • 95
    Thermo Fisher block it fluorescent sirna
    KYL-conjugated protamine increases the uptake of <t>siRNA</t> in LNCaP cells. Two and a half microliters of the <t>BLOCK-iT™</t> Fluorescent siRNA (20 μ M) were mixed with 1.5 μ g protamine (or the protamine-KYL conjugate) in 100 μ l serum-free
    Block It Fluorescent Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/block it fluorescent sirna/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    block it fluorescent sirna - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    97
    Thermo Fisher block it alexa fluor red fluorescent oligo control
    The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the <t>BLOCK-iT</t> <t>Alexa</t> Fluor Red Fluorescent <t>Oligo.</t> 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p
    Block It Alexa Fluor Red Fluorescent Oligo Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/block it alexa fluor red fluorescent oligo control/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    block it alexa fluor red fluorescent oligo control - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    KYL-conjugated protamine increases the uptake of siRNA in LNCaP cells. Two and a half microliters of the BLOCK-iT™ Fluorescent siRNA (20 μ M) were mixed with 1.5 μ g protamine (or the protamine-KYL conjugate) in 100 μ l serum-free

    Journal: Pharmaceutical research

    Article Title: Identification of a LNCaP-Specific Binding Peptide Using Phage Display

    doi: 10.1007/s11095-011-0469-7

    Figure Lengend Snippet: KYL-conjugated protamine increases the uptake of siRNA in LNCaP cells. Two and a half microliters of the BLOCK-iT™ Fluorescent siRNA (20 μ M) were mixed with 1.5 μ g protamine (or the protamine-KYL conjugate) in 100 μ l serum-free

    Article Snippet: The BLOCK-iT™ Fluorescent siRNA was obtained from Invitrogen (Carlsbad, CA).

    Techniques: Blocking Assay

    Nodal knockdown induces a decrease of cell proliferation and an increase of apoptosis in C18-4 cells. ( A ): Transfection efficiency was monitored by the uptake of BLOCK-iT ™ fluorescent oligo (green fluorescence, left panel) at 24 hours following

    Journal: Stem cells (Dayton, Ohio)

    Article Title: Nodal Signaling via an Autocrine Pathway Promotes Proliferation of Mouse Spermatogonial Stem/Progenitor Cells through Smad2/3 and Oct-4 Activation

    doi: 10.1002/stem.198

    Figure Lengend Snippet: Nodal knockdown induces a decrease of cell proliferation and an increase of apoptosis in C18-4 cells. ( A ): Transfection efficiency was monitored by the uptake of BLOCK-iT ™ fluorescent oligo (green fluorescence, left panel) at 24 hours following

    Article Snippet: We found that Nodal siRNAs could be efficiently transfected into C18-4 cells using Lipofectamine™ 2000 as shown by a high uptake of the BLOCK-iT™ fluorescent oligo, whose fluorescent signal correlates with the delivery of active siRNAs (Invitrogen).

    Techniques: Transfection, Blocking Assay, Fluorescence

    siRNA c-Src knockdown promoted N-cadherin junction maturation and lens cell differentiation/morphogenesis. A siRNA approach was used to knockdown c-Src expression (si c-Src) in primary quail embryo lens cell cultures. Control cultures were transfected with siCONTROL non-targeting siRNAs (labeled as siControl or si-Ctl). (A) Immunoblot analysis showed that c-Src was effectively knocked-down without blocking the expression of the SFK Fyn. (B) Phase contrast imaging at 48 hrs post-transfection showed that knockdown of c-Src blocked expansion of lens epithelial cells; iii and iv are higher magnification images of representative areas from i and ii , respectively. (C) Immunostaining for phosphoHistone3, a mitotic cell marker confirmed that the block in population expansion following c-Src siRNA knockdown resulted, at least in part, from the inhibition of lens cell proliferation (pHistone3, red; nuclei, blue); results quantified in the panel to the right. (D) Immunoblotting for filensin and aquaporin-0, with β-actin as control, showed that c-Src siRNA knockdown promoted lens differentiation-specific gene expression. (E) Phase contrast imaging at 72 hrs post-transfection showed that siRNA knockdown of c-Src promoted formation of lentoids (L, outlined with a dashed white line). (D) To examine if the c-Src siRNA knockdown affected maturation of N-cadherin junctions, primary quail embryo lens cultures were transfected with either c-Src siRNA or siCONTROL non-targeting siRNAs, and each co-transfected with the BLOCK-iT fluorescent oligo (green) to mark transfected cells. Confocal imaging following immunostaining for N-cadherin (red) showed that c-Src knockdown induced maturation of N-cadherin junctions, seen as linear staining for N-cadherin along cell-cell interfaces of BLOCK-iT-positive (green) cells. White arrows point to the same BLOCK-iT-positive cell in each set of images. All studies were representative of at least three independent experiments. Phase contrast images were acquired at 10X; magnification bar=20μm.

    Journal: Developmental dynamics : an official publication of the American Association of Anatomists

    Article Title: DISTINCT ROLES FOR N-CADHERIN LINKED C-SRC AND FYN KINASES IN LENS DEVELOPMENT

    doi: 10.1002/dvdy.23935

    Figure Lengend Snippet: siRNA c-Src knockdown promoted N-cadherin junction maturation and lens cell differentiation/morphogenesis. A siRNA approach was used to knockdown c-Src expression (si c-Src) in primary quail embryo lens cell cultures. Control cultures were transfected with siCONTROL non-targeting siRNAs (labeled as siControl or si-Ctl). (A) Immunoblot analysis showed that c-Src was effectively knocked-down without blocking the expression of the SFK Fyn. (B) Phase contrast imaging at 48 hrs post-transfection showed that knockdown of c-Src blocked expansion of lens epithelial cells; iii and iv are higher magnification images of representative areas from i and ii , respectively. (C) Immunostaining for phosphoHistone3, a mitotic cell marker confirmed that the block in population expansion following c-Src siRNA knockdown resulted, at least in part, from the inhibition of lens cell proliferation (pHistone3, red; nuclei, blue); results quantified in the panel to the right. (D) Immunoblotting for filensin and aquaporin-0, with β-actin as control, showed that c-Src siRNA knockdown promoted lens differentiation-specific gene expression. (E) Phase contrast imaging at 72 hrs post-transfection showed that siRNA knockdown of c-Src promoted formation of lentoids (L, outlined with a dashed white line). (D) To examine if the c-Src siRNA knockdown affected maturation of N-cadherin junctions, primary quail embryo lens cultures were transfected with either c-Src siRNA or siCONTROL non-targeting siRNAs, and each co-transfected with the BLOCK-iT fluorescent oligo (green) to mark transfected cells. Confocal imaging following immunostaining for N-cadherin (red) showed that c-Src knockdown induced maturation of N-cadherin junctions, seen as linear staining for N-cadherin along cell-cell interfaces of BLOCK-iT-positive (green) cells. White arrows point to the same BLOCK-iT-positive cell in each set of images. All studies were representative of at least three independent experiments. Phase contrast images were acquired at 10X; magnification bar=20μm.

    Article Snippet: As an indicator of transfected cells in the immunolocalization studies lens cultures were co-transfected with BLOCK-iT fluorescent oligo (Invitrogen, Camarillo, CA).

    Techniques: Cell Differentiation, Expressing, Transfection, Labeling, CTL Assay, Blocking Assay, Imaging, Immunostaining, Marker, Inhibition, Staining

    The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p

    Journal: Cells

    Article Title: miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B

    doi: 10.3390/cells8121548

    Figure Lengend Snippet: The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p

    Article Snippet: To optimize transfection efficacy, the BLOCK-iT Alexa Fluor Red Fluorescent Oligo control (Invitrogen) was transfected and fluorescence was measured 24 h post transfection (excitation 540 nm, emission 590 nm).

    Techniques: Transfection, Blocking Assay