block it alexa fluor red fluorescent oligo control  (Thermo Fisher)


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    Name:
    BLOCK iT Alexa Fluor Red Fluorescent Control
    Description:
    The BLOCK iT Alexa Fluor Red Fluorescent Control is an ideal indicator of lipid mediated transfection efficiency for RNAi experiments particularly those using the Lipofectamine RNAiMAX Transfection Reagent The sequence of the BLOCK iT Alexa Fluor Red Fluorescent Control is not homologous to any known gene preventing induction of nonspecific cellular events caused by introduction of the duplex into cells The BLOCK iT Alexa Fluor Red Fluorescent Control provides • A strong easily detectable and photostable Alexa Fluor 555 signal indicating transfection efficiency • Chemical modifications for a clearer more persistent cellular signal than with labeled siRNA • Proven correlation of transfection efficiency with Stealth RNAi siRNA and traditional siRNA molecules This dye can be detected using standard filter sets designed for Alexa Fluor 555 Cy 3 DsRed Texas Red or rhodamine fluorophores
    Catalog Number:
    14750100
    Price:
    None
    Category:
    Standards Ladders Controls
    Applications:
    Cell Culture|RNAi|RNAi Transfection|RNAi, Epigenetics & Non-Coding RNA Research|Synthetic siRNA Transfection|siRNA|Transfection
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    Structured Review

    Thermo Fisher block it alexa fluor red fluorescent oligo control
    The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the <t>BLOCK-iT</t> <t>Alexa</t> Fluor Red Fluorescent <t>Oligo.</t> 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p
    The BLOCK iT Alexa Fluor Red Fluorescent Control is an ideal indicator of lipid mediated transfection efficiency for RNAi experiments particularly those using the Lipofectamine RNAiMAX Transfection Reagent The sequence of the BLOCK iT Alexa Fluor Red Fluorescent Control is not homologous to any known gene preventing induction of nonspecific cellular events caused by introduction of the duplex into cells The BLOCK iT Alexa Fluor Red Fluorescent Control provides • A strong easily detectable and photostable Alexa Fluor 555 signal indicating transfection efficiency • Chemical modifications for a clearer more persistent cellular signal than with labeled siRNA • Proven correlation of transfection efficiency with Stealth RNAi siRNA and traditional siRNA molecules This dye can be detected using standard filter sets designed for Alexa Fluor 555 Cy 3 DsRed Texas Red or rhodamine fluorophores
    https://www.bioz.com/result/block it alexa fluor red fluorescent oligo control/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    block it alexa fluor red fluorescent oligo control - by Bioz Stars, 2021-04
    97/100 stars

    Images

    1) Product Images from "miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B"

    Article Title: miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B

    Journal: Cells

    doi: 10.3390/cells8121548

    The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p
    Figure Legend Snippet: The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p

    Techniques Used: Transfection, Blocking Assay

    Related Articles

    Transfection:

    Article Title: Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system
    Article Snippet: We then evaluated the effect of in vivo gene silencing of P2Y6 R using a small interfering RNA (siRNA) on the extension response. shows the diffusion area of the focally injected solution containing Evans blue on the surface of the somatosensory cortex. .. To optimize the conditions for gene silencing by siRNA, we first examined the transfection efficiency into microglia using BLOCK-iT Alexa Fluor Red Fluorescent Control (Thermo Fisher Scientific, Waltham, MA, USA). .. At 24 h after reverse transfection, the GFP-positive microglia in the cortical layers of 2–3 CX3CR1 +/GFP mice corresponded well with the BLOCK-iT Alexa Fluor Red-positive cells ( , arrowheads).

    Article Title: Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch
    Article Snippet: This line was established by successive use of Tol2 transposition, drug selection with G418 (300 μg/ml, Sigma) and picking of RFP-positive clones. .. In some experiments, 50 ng of non-integrative plasmid expressing an FP marker distinct from the iOn vector (CMV::mTurquoise2 , CMV::IRFP or CAG::GFP) were applied as transfection control. .. For FACS analysis, transfections were performed in 6-cm dishes with scaled up concentrations.

    Article Title: TREM-1 Protects HIV-1-Infected Macrophages from Apoptosis through Maintenance of Mitochondrial Function
    Article Snippet: .. Transfection efficiency was assessed with BLOCK-iT Alexa Fluor red fluorescent control (Thermo Fisher) using flow cytometry. ..

    Article Title: ELF4 is a target of miR-124 and promotes neuroblastoma proliferation and undifferentiated state
    Article Snippet: Firefly luciferase luminescence was measured 48 hours later using the Dual-Glo® Luciferase Assay System (Promega). .. Sea pansy luminescence was measured as a transfection control. .. RNA-seq AnalysisTo identify the transcriptional impact of ELF4, BE(2)-C cells were treated in triplicate with either siELF4 or siControl.

    Article Title: Helium alters the cytoskeleton and decreases permeability in endothelial cells cultured in vitro through a pathway involving Caveolin-1
    Article Snippet: .. Transfection with Block-iT Alexa Flour Red HUVEC growing on glass cover slips at a confluency of 50–80% were transfected with the red fluorescent protein Block-iT Alexa Flour Red (Invitrogen by Thermo Fischer Scientifics, Waltham, MA, USA) with use of Lipofectamine RNAiMAX (Invitrogen by Thermo Fischer Scientifics, Waltham, MA, USA). .. Block-iT Alexa Flour Red was used at a final concentration of 100 nM.

    Article Title: LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma
    Article Snippet: HNE1 and HONE1 cells were transfected with linc00312 overexpression vector or control vector for 24 h using Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA). .. Then, the cells were transfected with 1 μg of either the linearized plasmid or uncut pGL3 control plasmid, along with 50 ng of Renilla luciferase vector as a transfection efficiency control. .. Luciferase activity was assayed using the Dual Luciferase Reporter Assay Kit (Promega) 24 h after transfection.

    Article Title: The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch
    Article Snippet: Similar experiments were performed in wild-type embryos assaying Dlx2 mRNA expression in response to Wnt5a-soaked beads. .. RNA was prepared from PAs transfected with miR-452 mimic and Block-iT Alexa Fluor-red (Invitrogen) or Block-iT alone, using Trizol. .. For miRNA qRT-PCR, cDNA was reverse transcribed from 10 ng total RNA using the TaqMan microRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). miR-16 and U6 small nuclear RNA served as endogenous controls.

    Blocking Assay:

    Article Title: Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system
    Article Snippet: We then evaluated the effect of in vivo gene silencing of P2Y6 R using a small interfering RNA (siRNA) on the extension response. shows the diffusion area of the focally injected solution containing Evans blue on the surface of the somatosensory cortex. .. To optimize the conditions for gene silencing by siRNA, we first examined the transfection efficiency into microglia using BLOCK-iT Alexa Fluor Red Fluorescent Control (Thermo Fisher Scientific, Waltham, MA, USA). .. At 24 h after reverse transfection, the GFP-positive microglia in the cortical layers of 2–3 CX3CR1 +/GFP mice corresponded well with the BLOCK-iT Alexa Fluor Red-positive cells ( , arrowheads).

    Article Title: TREM-1 Protects HIV-1-Infected Macrophages from Apoptosis through Maintenance of Mitochondrial Function
    Article Snippet: .. Transfection efficiency was assessed with BLOCK-iT Alexa Fluor red fluorescent control (Thermo Fisher) using flow cytometry. ..

    Article Title: Helium alters the cytoskeleton and decreases permeability in endothelial cells cultured in vitro through a pathway involving Caveolin-1
    Article Snippet: .. Transfection with Block-iT Alexa Flour Red HUVEC growing on glass cover slips at a confluency of 50–80% were transfected with the red fluorescent protein Block-iT Alexa Flour Red (Invitrogen by Thermo Fischer Scientifics, Waltham, MA, USA) with use of Lipofectamine RNAiMAX (Invitrogen by Thermo Fischer Scientifics, Waltham, MA, USA). .. Block-iT Alexa Flour Red was used at a final concentration of 100 nM.

    Article Title: Balancing polymer hydrophobicity for ligand presentation and siRNA delivery in dual function CXCR4 inhibiting polyplexes
    Article Snippet: PAMD-Ch polymers were fluorescently labelled with AlexaFluor 647 following manufacturer’s instructions and purified by dialysis to remove unreacted dye. .. Fluorescently labelled siRNA (Block-iT™ Alexa Fluor® Red) was purchased from Invitrogen (Carlsbad, CA). ..

    Article Title: The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch
    Article Snippet: Similar experiments were performed in wild-type embryos assaying Dlx2 mRNA expression in response to Wnt5a-soaked beads. .. RNA was prepared from PAs transfected with miR-452 mimic and Block-iT Alexa Fluor-red (Invitrogen) or Block-iT alone, using Trizol. .. For miRNA qRT-PCR, cDNA was reverse transcribed from 10 ng total RNA using the TaqMan microRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). miR-16 and U6 small nuclear RNA served as endogenous controls.

    Plasmid Preparation:

    Article Title: Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch
    Article Snippet: This line was established by successive use of Tol2 transposition, drug selection with G418 (300 μg/ml, Sigma) and picking of RFP-positive clones. .. In some experiments, 50 ng of non-integrative plasmid expressing an FP marker distinct from the iOn vector (CMV::mTurquoise2 , CMV::IRFP or CAG::GFP) were applied as transfection control. .. For FACS analysis, transfections were performed in 6-cm dishes with scaled up concentrations.

    Article Title: LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma
    Article Snippet: HNE1 and HONE1 cells were transfected with linc00312 overexpression vector or control vector for 24 h using Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA). .. Then, the cells were transfected with 1 μg of either the linearized plasmid or uncut pGL3 control plasmid, along with 50 ng of Renilla luciferase vector as a transfection efficiency control. .. Luciferase activity was assayed using the Dual Luciferase Reporter Assay Kit (Promega) 24 h after transfection.

    Expressing:

    Article Title: Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch
    Article Snippet: This line was established by successive use of Tol2 transposition, drug selection with G418 (300 μg/ml, Sigma) and picking of RFP-positive clones. .. In some experiments, 50 ng of non-integrative plasmid expressing an FP marker distinct from the iOn vector (CMV::mTurquoise2 , CMV::IRFP or CAG::GFP) were applied as transfection control. .. For FACS analysis, transfections were performed in 6-cm dishes with scaled up concentrations.

    Marker:

    Article Title: Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch
    Article Snippet: This line was established by successive use of Tol2 transposition, drug selection with G418 (300 μg/ml, Sigma) and picking of RFP-positive clones. .. In some experiments, 50 ng of non-integrative plasmid expressing an FP marker distinct from the iOn vector (CMV::mTurquoise2 , CMV::IRFP or CAG::GFP) were applied as transfection control. .. For FACS analysis, transfections were performed in 6-cm dishes with scaled up concentrations.

    Flow Cytometry:

    Article Title: TREM-1 Protects HIV-1-Infected Macrophages from Apoptosis through Maintenance of Mitochondrial Function
    Article Snippet: .. Transfection efficiency was assessed with BLOCK-iT Alexa Fluor red fluorescent control (Thermo Fisher) using flow cytometry. ..

    Cytometry:

    Article Title: TREM-1 Protects HIV-1-Infected Macrophages from Apoptosis through Maintenance of Mitochondrial Function
    Article Snippet: .. Transfection efficiency was assessed with BLOCK-iT Alexa Fluor red fluorescent control (Thermo Fisher) using flow cytometry. ..

    Luciferase:

    Article Title: LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma
    Article Snippet: HNE1 and HONE1 cells were transfected with linc00312 overexpression vector or control vector for 24 h using Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA). .. Then, the cells were transfected with 1 μg of either the linearized plasmid or uncut pGL3 control plasmid, along with 50 ng of Renilla luciferase vector as a transfection efficiency control. .. Luciferase activity was assayed using the Dual Luciferase Reporter Assay Kit (Promega) 24 h after transfection.

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  • 97
    Thermo Fisher control sirna
    Repressive H3K27 trimethylation is reduced in <t>Dnmt1</t> -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 <t>siRNA</t> (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P
    Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control sirna/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control sirna - by Bioz Stars, 2021-04
    97/100 stars
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    Image Search Results


    Repressive H3K27 trimethylation is reduced in Dnmt1 -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 siRNA (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P

    Journal: Epigenetics

    Article Title: DNMT1 modulates interneuron morphology by regulating Pak6 expression through crosstalk with histone modifications

    doi: 10.1080/15592294.2018.1475980

    Figure Lengend Snippet: Repressive H3K27 trimethylation is reduced in Dnmt1 -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 siRNA (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P

    Article Snippet: Mouse Dnmt1 siRNA oligos (50 nM) or mouse Pak6 siRNA oligos (50 nM), containing a pool of at least three target-specific 19–25 nt siRNAs to knockdown gene expression ( Dnmt1 siRNA sc-35,203; Pak6 siRNA sc-44,879; Santa Cruz Biotechnology) or 50 nM control siRNA (BLOCK-iT Alexa Fluor red/green fluorescent oligo; Invitrogen) were applied for 5 h in antibiotic-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) at 37°C, 5% CO2 , and 95% humidity.

    Techniques: Staining, Fluorescence, Western Blot, Immunoprecipitation

    Dnmt1 -depletion causes a reduced association of trimethylated H3K27 with Pak6 regulatory regions. (A) Schematic illustration of the Pak6 gene locus with promoter (red), promoter flanking (light red), enhancer (yellow) and non-regulatory (white) sites according to UCSC genome browser showing specific DNA primer positions (#1, #2, #3) in regulatory (#1, #2) and non-regulatory (#3) gene regions. (B, C) ChIP-analysis of control and Dnmt1 siRNA-treated N2a cells with a H3K27me3-specific antibody shows the association of H3K27me3 to the indicated primer locations (#1–3 in A) in the Pak6 gene (B), analyzed by quantitative PCR and normalized to the amount of input (C; three individual experiments with four technical replicates each). IgG controls indicate the amount of non-specifically bound DNA. ***P

    Journal: Epigenetics

    Article Title: DNMT1 modulates interneuron morphology by regulating Pak6 expression through crosstalk with histone modifications

    doi: 10.1080/15592294.2018.1475980

    Figure Lengend Snippet: Dnmt1 -depletion causes a reduced association of trimethylated H3K27 with Pak6 regulatory regions. (A) Schematic illustration of the Pak6 gene locus with promoter (red), promoter flanking (light red), enhancer (yellow) and non-regulatory (white) sites according to UCSC genome browser showing specific DNA primer positions (#1, #2, #3) in regulatory (#1, #2) and non-regulatory (#3) gene regions. (B, C) ChIP-analysis of control and Dnmt1 siRNA-treated N2a cells with a H3K27me3-specific antibody shows the association of H3K27me3 to the indicated primer locations (#1–3 in A) in the Pak6 gene (B), analyzed by quantitative PCR and normalized to the amount of input (C; three individual experiments with four technical replicates each). IgG controls indicate the amount of non-specifically bound DNA. ***P

    Article Snippet: Mouse Dnmt1 siRNA oligos (50 nM) or mouse Pak6 siRNA oligos (50 nM), containing a pool of at least three target-specific 19–25 nt siRNAs to knockdown gene expression ( Dnmt1 siRNA sc-35,203; Pak6 siRNA sc-44,879; Santa Cruz Biotechnology) or 50 nM control siRNA (BLOCK-iT Alexa Fluor red/green fluorescent oligo; Invitrogen) were applied for 5 h in antibiotic-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) at 37°C, 5% CO2 , and 95% humidity.

    Techniques: Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

    Enhanced Pak6 expression induced by EZH2 inhibition results in increased morphological complexity. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with DMSO (A, C) or DZNep (B, D) and stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). Quantification of cell morphology is shown in (E) as complexity index relative to DMSO controls. The complexity index is a product of primary neurite number, the branch point number of the longest process and the ratio between longest process length and the mean length of all other neurites. (E; n = 52 POA and 85 N2a cells for DMSO, n = 69 POA and 93 N2a cells for DZNep). (F-J) Representative microphotographs of dissociated E16 (+ 2 div) POA cells treated either with DMSO (F, H) or DZNep (G, I) in addition to control (F, G) or Pak6 siRNA (H, I), then stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). The complexity index is quantified in (J) relative to DMSO + ctrl siRNA cell parameters (n = 39 POA cells for DMSO + ctrl siRNA, n = 43 POA cells for DZNep + ctrl siRNA, n = 51 POA cells for DMSO + Pak6 siRNA, n = 68 POA cells for DZNep + Pak6 siRNA). ‘n’ refers to the number of analyzed cells. **P

    Journal: Epigenetics

    Article Title: DNMT1 modulates interneuron morphology by regulating Pak6 expression through crosstalk with histone modifications

    doi: 10.1080/15592294.2018.1475980

    Figure Lengend Snippet: Enhanced Pak6 expression induced by EZH2 inhibition results in increased morphological complexity. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with DMSO (A, C) or DZNep (B, D) and stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). Quantification of cell morphology is shown in (E) as complexity index relative to DMSO controls. The complexity index is a product of primary neurite number, the branch point number of the longest process and the ratio between longest process length and the mean length of all other neurites. (E; n = 52 POA and 85 N2a cells for DMSO, n = 69 POA and 93 N2a cells for DZNep). (F-J) Representative microphotographs of dissociated E16 (+ 2 div) POA cells treated either with DMSO (F, H) or DZNep (G, I) in addition to control (F, G) or Pak6 siRNA (H, I), then stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). The complexity index is quantified in (J) relative to DMSO + ctrl siRNA cell parameters (n = 39 POA cells for DMSO + ctrl siRNA, n = 43 POA cells for DZNep + ctrl siRNA, n = 51 POA cells for DMSO + Pak6 siRNA, n = 68 POA cells for DZNep + Pak6 siRNA). ‘n’ refers to the number of analyzed cells. **P

    Article Snippet: Mouse Dnmt1 siRNA oligos (50 nM) or mouse Pak6 siRNA oligos (50 nM), containing a pool of at least three target-specific 19–25 nt siRNAs to knockdown gene expression ( Dnmt1 siRNA sc-35,203; Pak6 siRNA sc-44,879; Santa Cruz Biotechnology) or 50 nM control siRNA (BLOCK-iT Alexa Fluor red/green fluorescent oligo; Invitrogen) were applied for 5 h in antibiotic-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) at 37°C, 5% CO2 , and 95% humidity.

    Techniques: Expressing, Inhibition, Staining, Labeling

    The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p

    Journal: Cells

    Article Title: miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B

    doi: 10.3390/cells8121548

    Figure Lengend Snippet: The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p

    Article Snippet: To optimize transfection efficacy, the BLOCK-iT Alexa Fluor Red Fluorescent Oligo control (Invitrogen) was transfected and fluorescence was measured 24 h post transfection (excitation 540 nm, emission 590 nm).

    Techniques: Transfection, Blocking Assay

    Cu/Zn siRNA decreased Cu/Zn SOD-positive spermatogonia and increased oxidative damage and mortality. (A) Photomicrographs of fluorescence immunohistochemistry of siRNA transfected spermatogonia after 3 days of culture. Green staining indicates Cu/Zn SOD positive signal, red staining indicates 8-OHdG positive signal. Arrows indicate Cu/Zn SOD-negative cells in Cu/Zn SOD immunohistochemitry or 8-OHdG-positive cells in 8-OHdG immunohistochemistry. BF, bright field. (B) Calculated number of spermatogonia expressing Cu/Zn SOD and positive for 8-OHdG after siRNA transfection (n = 5 per group, P

    Journal: PLoS ONE

    Article Title: Tolerance of Spermatogonia to Oxidative Stress Is Due to High Levels of Zn and Cu/Zn Superoxide Dismutase

    doi: 10.1371/journal.pone.0016938

    Figure Lengend Snippet: Cu/Zn siRNA decreased Cu/Zn SOD-positive spermatogonia and increased oxidative damage and mortality. (A) Photomicrographs of fluorescence immunohistochemistry of siRNA transfected spermatogonia after 3 days of culture. Green staining indicates Cu/Zn SOD positive signal, red staining indicates 8-OHdG positive signal. Arrows indicate Cu/Zn SOD-negative cells in Cu/Zn SOD immunohistochemitry or 8-OHdG-positive cells in 8-OHdG immunohistochemistry. BF, bright field. (B) Calculated number of spermatogonia expressing Cu/Zn SOD and positive for 8-OHdG after siRNA transfection (n = 5 per group, P

    Article Snippet: Briefly, 15 pmol of eCu/Zn-SOD or control siRNA (BLOCK-iT™Alexa Flour® Red Fluorescent Oligo, Invitrogen) were diluted in 50 µl Opti-MEM I Reduced Serum Medium (Invitrogen) and then mixed.

    Techniques: Fluorescence, Immunohistochemistry, Transfection, Staining, Expressing

    The effect of mouse Oct4 knockdown in CCE cells. a and b CCE cells were transfected without ( a ) or with ( b ) the negative control alone for 48 h. c More than 85% of the cells were successfully transfected with Alexa Fluor Red Fluorescent Oligo. d , e and f The self-renewal and undifferentiated state of CCE cells were not maintained when transfected with the three siRNA of Oct4 – R1 ( d ), R2 ( e ) and R3 ( f ) when tested 48 h after the transfection. The arrows indicate the normal CCE mouse ES cells. The images were photographed with Leica DMI3000B at 200× magnification. Scale bar = 20 μm

    Journal: Cellular & Molecular Biology Letters

    Article Title: Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element

    doi: 10.1186/s11658-018-0104-2

    Figure Lengend Snippet: The effect of mouse Oct4 knockdown in CCE cells. a and b CCE cells were transfected without ( a ) or with ( b ) the negative control alone for 48 h. c More than 85% of the cells were successfully transfected with Alexa Fluor Red Fluorescent Oligo. d , e and f The self-renewal and undifferentiated state of CCE cells were not maintained when transfected with the three siRNA of Oct4 – R1 ( d ), R2 ( e ) and R3 ( f ) when tested 48 h after the transfection. The arrows indicate the normal CCE mouse ES cells. The images were photographed with Leica DMI3000B at 200× magnification. Scale bar = 20 μm

    Article Snippet: BLOCK-It Alexa Fluor Red Fluorescent Oligo (Invitrogen) was used to facilitate the assessment and optimize the delivery of double-stranded RNA oligonucleotides into the CCE cells.

    Techniques: Transfection, Negative Control