block it alexa fluor red fluorescent oligo control (Thermo Fisher)


Name:
BLOCK iT Alexa Fluor Red Fluorescent Control
Description:
The BLOCK iT Alexa Fluor Red Fluorescent Control is an ideal indicator of lipid mediated transfection efficiency for RNAi experiments particularly those using the Lipofectamine RNAiMAX Transfection Reagent The sequence of the BLOCK iT Alexa Fluor Red Fluorescent Control is not homologous to any known gene preventing induction of nonspecific cellular events caused by introduction of the duplex into cells The BLOCK iT Alexa Fluor Red Fluorescent Control provides • A strong easily detectable and photostable Alexa Fluor 555 signal indicating transfection efficiency • Chemical modifications for a clearer more persistent cellular signal than with labeled siRNA • Proven correlation of transfection efficiency with Stealth RNAi siRNA and traditional siRNA molecules This dye can be detected using standard filter sets designed for Alexa Fluor 555 Cy 3 DsRed Texas Red or rhodamine fluorophores
Catalog Number:
14750100
Price:
None
Category:
Standards Ladders Controls
Applications:
Cell Culture|RNAi|RNAi Transfection|RNAi, Epigenetics & Non-Coding RNA Research|Synthetic siRNA Transfection|siRNA|Transfection
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Structured Review

The BLOCK iT Alexa Fluor Red Fluorescent Control is an ideal indicator of lipid mediated transfection efficiency for RNAi experiments particularly those using the Lipofectamine RNAiMAX Transfection Reagent The sequence of the BLOCK iT Alexa Fluor Red Fluorescent Control is not homologous to any known gene preventing induction of nonspecific cellular events caused by introduction of the duplex into cells The BLOCK iT Alexa Fluor Red Fluorescent Control provides • A strong easily detectable and photostable Alexa Fluor 555 signal indicating transfection efficiency • Chemical modifications for a clearer more persistent cellular signal than with labeled siRNA • Proven correlation of transfection efficiency with Stealth RNAi siRNA and traditional siRNA molecules This dye can be detected using standard filter sets designed for Alexa Fluor 555 Cy 3 DsRed Texas Red or rhodamine fluorophores
https://www.bioz.com/result/block it alexa fluor red fluorescent oligo control/product/Thermo Fisher
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Price from $9.99 to $1999.99
Images
1) Product Images from "miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B"
Article Title: miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B
Journal: Cells
doi: 10.3390/cells8121548

Figure Legend Snippet: The mirVana miRNA mimic transfections in THP-1 macrophage-like cells. ( a , b ) Transfection optimization using the BLOCK-iT Alexa Fluor Red Fluorescent Oligo. 20 nM or 40 nM of the fluorescent oligo were transfected into THP-1 macrophage-like cells using Lipofectamine 2000 to confirm the positive transfection of small nucleotides in the cells, 24 h after transfection. Data are presented as the mean ± SEM of two different duplicate measurements divided in 5 wells. *** p
Techniques Used: Transfection, Blocking Assay
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