bl21 star de3 cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 star de3 cells
    Bl21 Star De3 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 star de3 cells/product/Thermo Fisher
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    bl21 star de3 cells - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    Construct:

    Article Title: A dynamic regulatory interface on SARS-CoV-2 RNA polymerase
    Article Snippet: .. All constructs were transformed into Escherichia coli BL21(DE3) competent cells. ..

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Purification:

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Article Title: Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth
    Article Snippet: .. Purification of the His6 -RABIN8 and direct binding assay His6 -RABIN8 were expressed in BL21(DE3) cells that had been induced with 1 m m IPTG at 37 °C for 4 h. Bacteria pellets were then sonicated in PBS with 1 mg/ml lysozyme. .. Proteins were then pelleted by centrifugation at 9000 rpm for 10 min and dissolved in PBS with 8 m urea.

    Concentration Assay:

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    other:

    Article Title: Mapping RNAPII CTD Phosphorylation Reveals That the Identity and Modification of Seventh Heptad Residues Direct Tyr1 Phosphorylation
    Article Snippet: BL21 (DE3) cells coexpressing the human ABL1 kinase domain (residues 229−511) and YopH phosphatase from Yersinia were a kind gift from the Kuriyan lab.

    Expressing:

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly (Bemisia tabaci)
    Article Snippet: .. Plasmids were transformed into BL21(DE3) cells for BtKV-Cas9/mCh expression following standard protein expression protocols. ..

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Sequencing:

    Article Title: Improving prognosis of surrogate assay for breast cancer patients by absolute quantitation of Ki67 protein levels using Quantitative Dot Blot (QDB) method
    Article Snippet: .. The plasmid was verified by sequencing,95 and expressed in BL21 (DE3) competent cells. .. The cells were induced with IPTG, and 96 total bacterial lysate was extracted in 10ml binding buffer (20 mM sodium phosphate, 97 500 mM NaCI, 20 mM imidazole, PH 7.4) before it was loaded onto a high affinity Ni2+ 98 column pre-equilibrated with 10ml binding buffer.

    Sonication:

    Article Title: Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth
    Article Snippet: .. Purification of the His6 -RABIN8 and direct binding assay His6 -RABIN8 were expressed in BL21(DE3) cells that had been induced with 1 m m IPTG at 37 °C for 4 h. Bacteria pellets were then sonicated in PBS with 1 mg/ml lysozyme. .. Proteins were then pelleted by centrifugation at 9000 rpm for 10 min and dissolved in PBS with 8 m urea.

    Transformation Assay:

    Article Title: A dynamic regulatory interface on SARS-CoV-2 RNA polymerase
    Article Snippet: .. All constructs were transformed into Escherichia coli BL21(DE3) competent cells. ..

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly (Bemisia tabaci)
    Article Snippet: .. Plasmids were transformed into BL21(DE3) cells for BtKV-Cas9/mCh expression following standard protein expression protocols. ..

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Article Title: Glucosamine-6P and glucosamine-1P, respectively an activator and a substrate of rhodococcal ADP-glucose pyrophosphorylases, show a hint to ascertain (actino)bacterial glucosamine metabolism
    Article Snippet: .. Competent E. coli BL21 (DE3) cells were transformed with the [pET28c/RfaglgC ] construction. .. Protein expression was carried out using LB medium (10 g/l tryptone; 5 g/l yeast extract; 5 g/l NaCl) supplemented with 100 g/ml kanamycin.

    Binding Assay:

    Article Title: Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth
    Article Snippet: .. Purification of the His6 -RABIN8 and direct binding assay His6 -RABIN8 were expressed in BL21(DE3) cells that had been induced with 1 m m IPTG at 37 °C for 4 h. Bacteria pellets were then sonicated in PBS with 1 mg/ml lysozyme. .. Proteins were then pelleted by centrifugation at 9000 rpm for 10 min and dissolved in PBS with 8 m urea.

    Plasmid Preparation:

    Article Title: Improving prognosis of surrogate assay for breast cancer patients by absolute quantitation of Ki67 protein levels using Quantitative Dot Blot (QDB) method
    Article Snippet: .. The plasmid was verified by sequencing,95 and expressed in BL21 (DE3) competent cells. .. The cells were induced with IPTG, and 96 total bacterial lysate was extracted in 10ml binding buffer (20 mM sodium phosphate, 97 500 mM NaCI, 20 mM imidazole, PH 7.4) before it was loaded onto a high affinity Ni2+ 98 column pre-equilibrated with 10ml binding buffer.

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

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  • 99
    Thermo Fisher bl21 cells
    Role of BCAS0292 in epithelial cell attachment. A) Attachment of K56-2 WT, ΔBCAS0292 mutant and Δ0292_0292 complemented strain to CFBE41o − cells at an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain with 20 fields of view counted per data point in three independent experiments, ** p =0.0073. B) Confocal microscopy images representing the attachment of E. coli <t>BL21</t> control and E. coli BL21_BCAS0292 strains to CFBE41o-cells. Bacteria were labelled using a primary anti- E. coli FITC-conjugated antibody (green). Nuclei of CFBE41o-cells were counterstained with DAPI (blue) and actin stained with phalloidin conjugated with Alexa fluor 568. C) Attachment E. coli BL21 control and E. coli BL21_BCAS0292 to CFBE41o-cells, using an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain, with 20 fields of view counted per data point in three independent experiments. D) Confirmation of expression of BCAS0292, following induction in E. coli BL21 cells with 1mM IPTG by Coomassie Blue stained SDS PAGE gel (conditions used for Figure 1C ). Lane 1: MW marker; Lane 2: E. coli BL21 expression control; Lane 3: E. coli BL21_BCAS0292 after incubation with IPTG (1mM) for 2 h.
    Bl21 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 cells/product/Thermo Fisher
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    bl21 cells - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher e coli bl21 cells
    Spot assays used for monitoring the growth performance of <t>BL21/pEZYHb</t> and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress
    E Coli Bl21 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 cells/product/Thermo Fisher
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 cells - by Bioz Stars, 2020-09
    99/100 stars
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    84
    Thermo Fisher purification competent bl21 star de3 e coli cells
    Spot assays used for monitoring the growth performance of <t>BL21/pEZYHb</t> and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress
    Purification Competent Bl21 Star De3 E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/purification competent bl21 star de3 e coli cells/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    purification competent bl21 star de3 e coli cells - by Bioz Stars, 2020-09
    84/100 stars
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    Image Search Results


    Role of BCAS0292 in epithelial cell attachment. A) Attachment of K56-2 WT, ΔBCAS0292 mutant and Δ0292_0292 complemented strain to CFBE41o − cells at an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain with 20 fields of view counted per data point in three independent experiments, ** p =0.0073. B) Confocal microscopy images representing the attachment of E. coli BL21 control and E. coli BL21_BCAS0292 strains to CFBE41o-cells. Bacteria were labelled using a primary anti- E. coli FITC-conjugated antibody (green). Nuclei of CFBE41o-cells were counterstained with DAPI (blue) and actin stained with phalloidin conjugated with Alexa fluor 568. C) Attachment E. coli BL21 control and E. coli BL21_BCAS0292 to CFBE41o-cells, using an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain, with 20 fields of view counted per data point in three independent experiments. D) Confirmation of expression of BCAS0292, following induction in E. coli BL21 cells with 1mM IPTG by Coomassie Blue stained SDS PAGE gel (conditions used for Figure 1C ). Lane 1: MW marker; Lane 2: E. coli BL21 expression control; Lane 3: E. coli BL21_BCAS0292 after incubation with IPTG (1mM) for 2 h.

    Journal: bioRxiv

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia

    doi: 10.1101/2020.08.06.226779

    Figure Lengend Snippet: Role of BCAS0292 in epithelial cell attachment. A) Attachment of K56-2 WT, ΔBCAS0292 mutant and Δ0292_0292 complemented strain to CFBE41o − cells at an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain with 20 fields of view counted per data point in three independent experiments, ** p =0.0073. B) Confocal microscopy images representing the attachment of E. coli BL21 control and E. coli BL21_BCAS0292 strains to CFBE41o-cells. Bacteria were labelled using a primary anti- E. coli FITC-conjugated antibody (green). Nuclei of CFBE41o-cells were counterstained with DAPI (blue) and actin stained with phalloidin conjugated with Alexa fluor 568. C) Attachment E. coli BL21 control and E. coli BL21_BCAS0292 to CFBE41o-cells, using an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain, with 20 fields of view counted per data point in three independent experiments. D) Confirmation of expression of BCAS0292, following induction in E. coli BL21 cells with 1mM IPTG by Coomassie Blue stained SDS PAGE gel (conditions used for Figure 1C ). Lane 1: MW marker; Lane 2: E. coli BL21 expression control; Lane 3: E. coli BL21_BCAS0292 after incubation with IPTG (1mM) for 2 h.

    Article Snippet: Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C.

    Techniques: Cell Attachment Assay, Mutagenesis, Confocal Microscopy, Staining, Expressing, SDS Page, Marker, Incubation

    Spot assays used for monitoring the growth performance of BL21/pEZYHb and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress

    Journal: BMC Genomics

    Article Title: The alcohol dehydrogenase gene family in sugarcane and its involvement in cold stress regulation

    doi: 10.1186/s12864-020-06929-9

    Figure Lengend Snippet: Spot assays used for monitoring the growth performance of BL21/pEZYHb and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress

    Article Snippet: When E. coli BL21 cells containing different vectors were grown to OD600 of 0.6, 1.0 mmol L− 1 isopropyl β-D-thiogalactoside (IPTG) was added to induce protein production.

    Techniques: Transformation Assay, Incubation