bl21 star cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 star cells
    Bl21 Star Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 star cells/product/Thermo Fisher
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    bl21 star cells - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mining Endonuclease Cleavage Determinants in Genomic Sequence Data *
    Article Snippet: .. Expression and Purification of Proteins Genes for each homologue-based variant of I-AniI were assembled, cloned, sequence verified, and transformed into BL21 Star cells (Invitrogen). .. A half-liter or 1 liter culture of autoinduction media ( ) was then inoculated and grown at 37 °C for 8–12 h until approximate saturation, after which expression at 18 °C was allowed for 20–24 h. Cells were then harvested and resuspended in 20 mm Tris, pH 7.5, 30 mm imidazole, and 1.0 m NaCl prior to lysis via a freeze-thaw cycle, sonication, and the addition of lysozyme.

    Positron Emission Tomography:

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: .. C-terminally hexahistidine-tagged 16E1^E4 with a leucine-glutamic acid linker (His-16E1^E4) was expressed from the pET-28(b)+/16E1^E4 expression vector in BL21 Star cells (Invitrogen, United Kingdom) as described by the manufacturer. ..

    Purification:

    Article Title: Mining Endonuclease Cleavage Determinants in Genomic Sequence Data *
    Article Snippet: .. Expression and Purification of Proteins Genes for each homologue-based variant of I-AniI were assembled, cloned, sequence verified, and transformed into BL21 Star cells (Invitrogen). .. A half-liter or 1 liter culture of autoinduction media ( ) was then inoculated and grown at 37 °C for 8–12 h until approximate saturation, after which expression at 18 °C was allowed for 20–24 h. Cells were then harvested and resuspended in 20 mm Tris, pH 7.5, 30 mm imidazole, and 1.0 m NaCl prior to lysis via a freeze-thaw cycle, sonication, and the addition of lysozyme.

    Concentration Assay:

    Article Title: A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis
    Article Snippet: .. Gene expression in BL21 Star cells (Invitrogen) was induced by adding IPTG to a final concentration of 1 mM, and cultures were grown at 18°C overnight. .. Pt UGEc was purified from the supernatant of lysed cell pellets using HIS-Select Nickel Affinity Gel purification (Sigma-Aldrich).

    Generated:

    Article Title: Organochlorine-mediated potentiation of the general coactivator p300 through p38 mitogen-activated protein kinase
    Article Snippet: .. The GST and GST-coactivator fusion proteins were generated using pGEX expression vectors transformed into BL21 star cells (Invitrogen). ..

    Expressing:

    Article Title: A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis
    Article Snippet: .. Gene expression in BL21 Star cells (Invitrogen) was induced by adding IPTG to a final concentration of 1 mM, and cultures were grown at 18°C overnight. .. Pt UGEc was purified from the supernatant of lysed cell pellets using HIS-Select Nickel Affinity Gel purification (Sigma-Aldrich).

    Article Title: Organochlorine-mediated potentiation of the general coactivator p300 through p38 mitogen-activated protein kinase
    Article Snippet: .. The GST and GST-coactivator fusion proteins were generated using pGEX expression vectors transformed into BL21 star cells (Invitrogen). ..

    Article Title: Hyperunstable matrix proteins in the byssus of Mytilus galloprovincialis
    Article Snippet: .. A partial cDNA encoding the C-terminal 155 amino acids of a variant of the TMP family was subcloned into the pRSET expression vector (Invitrogen) and subsequently expressed in BL21 Star cells (Invitrogen). .. Expression of the recombinant protein was induced by adding isopropyl thiogalactoside (IPTG) to a final concentration of 1 mmol l–1 when the bacterial cultures reached an optical density at 600 nm (OD600 ) between 0.4 and 0.6.

    Article Title: Crystal Structures of Physcomitrella patens AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 [W] AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 [W] [OA]
    Article Snippet: .. For expression, the respective plasmid was transformed in BL21 Star cells (Invitrogen). ..

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: .. C-terminally hexahistidine-tagged 16E1^E4 with a leucine-glutamic acid linker (His-16E1^E4) was expressed from the pET-28(b)+/16E1^E4 expression vector in BL21 Star cells (Invitrogen, United Kingdom) as described by the manufacturer. ..

    Article Title: Mining Endonuclease Cleavage Determinants in Genomic Sequence Data *
    Article Snippet: .. Expression and Purification of Proteins Genes for each homologue-based variant of I-AniI were assembled, cloned, sequence verified, and transformed into BL21 Star cells (Invitrogen). .. A half-liter or 1 liter culture of autoinduction media ( ) was then inoculated and grown at 37 °C for 8–12 h until approximate saturation, after which expression at 18 °C was allowed for 20–24 h. Cells were then harvested and resuspended in 20 mm Tris, pH 7.5, 30 mm imidazole, and 1.0 m NaCl prior to lysis via a freeze-thaw cycle, sonication, and the addition of lysozyme.

    Sequencing:

    Article Title: Mining Endonuclease Cleavage Determinants in Genomic Sequence Data *
    Article Snippet: .. Expression and Purification of Proteins Genes for each homologue-based variant of I-AniI were assembled, cloned, sequence verified, and transformed into BL21 Star cells (Invitrogen). .. A half-liter or 1 liter culture of autoinduction media ( ) was then inoculated and grown at 37 °C for 8–12 h until approximate saturation, after which expression at 18 °C was allowed for 20–24 h. Cells were then harvested and resuspended in 20 mm Tris, pH 7.5, 30 mm imidazole, and 1.0 m NaCl prior to lysis via a freeze-thaw cycle, sonication, and the addition of lysozyme.

    Transformation Assay:

    Article Title: Organochlorine-mediated potentiation of the general coactivator p300 through p38 mitogen-activated protein kinase
    Article Snippet: .. The GST and GST-coactivator fusion proteins were generated using pGEX expression vectors transformed into BL21 star cells (Invitrogen). ..

    Article Title: Crystal Structures of Physcomitrella patens AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 [W] AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 [W] [OA]
    Article Snippet: .. For expression, the respective plasmid was transformed in BL21 Star cells (Invitrogen). ..

    Article Title: Mining Endonuclease Cleavage Determinants in Genomic Sequence Data *
    Article Snippet: .. Expression and Purification of Proteins Genes for each homologue-based variant of I-AniI were assembled, cloned, sequence verified, and transformed into BL21 Star cells (Invitrogen). .. A half-liter or 1 liter culture of autoinduction media ( ) was then inoculated and grown at 37 °C for 8–12 h until approximate saturation, after which expression at 18 °C was allowed for 20–24 h. Cells were then harvested and resuspended in 20 mm Tris, pH 7.5, 30 mm imidazole, and 1.0 m NaCl prior to lysis via a freeze-thaw cycle, sonication, and the addition of lysozyme.

    Variant Assay:

    Article Title: Hyperunstable matrix proteins in the byssus of Mytilus galloprovincialis
    Article Snippet: .. A partial cDNA encoding the C-terminal 155 amino acids of a variant of the TMP family was subcloned into the pRSET expression vector (Invitrogen) and subsequently expressed in BL21 Star cells (Invitrogen). .. Expression of the recombinant protein was induced by adding isopropyl thiogalactoside (IPTG) to a final concentration of 1 mmol l–1 when the bacterial cultures reached an optical density at 600 nm (OD600 ) between 0.4 and 0.6.

    Article Title: Mining Endonuclease Cleavage Determinants in Genomic Sequence Data *
    Article Snippet: .. Expression and Purification of Proteins Genes for each homologue-based variant of I-AniI were assembled, cloned, sequence verified, and transformed into BL21 Star cells (Invitrogen). .. A half-liter or 1 liter culture of autoinduction media ( ) was then inoculated and grown at 37 °C for 8–12 h until approximate saturation, after which expression at 18 °C was allowed for 20–24 h. Cells were then harvested and resuspended in 20 mm Tris, pH 7.5, 30 mm imidazole, and 1.0 m NaCl prior to lysis via a freeze-thaw cycle, sonication, and the addition of lysozyme.

    Plasmid Preparation:

    Article Title: Hyperunstable matrix proteins in the byssus of Mytilus galloprovincialis
    Article Snippet: .. A partial cDNA encoding the C-terminal 155 amino acids of a variant of the TMP family was subcloned into the pRSET expression vector (Invitrogen) and subsequently expressed in BL21 Star cells (Invitrogen). .. Expression of the recombinant protein was induced by adding isopropyl thiogalactoside (IPTG) to a final concentration of 1 mmol l–1 when the bacterial cultures reached an optical density at 600 nm (OD600 ) between 0.4 and 0.6.

    Article Title: Crystal Structures of Physcomitrella patens AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 [W] AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity 1 [W] [OA]
    Article Snippet: .. For expression, the respective plasmid was transformed in BL21 Star cells (Invitrogen). ..

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: .. C-terminally hexahistidine-tagged 16E1^E4 with a leucine-glutamic acid linker (His-16E1^E4) was expressed from the pET-28(b)+/16E1^E4 expression vector in BL21 Star cells (Invitrogen, United Kingdom) as described by the manufacturer. ..

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    Thermo Fisher bl21 cells
    Role of BCAS0292 in epithelial cell attachment. A) Attachment of K56-2 WT, ΔBCAS0292 mutant and Δ0292_0292 complemented strain to CFBE41o − cells at an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain with 20 fields of view counted per data point in three independent experiments, ** p =0.0073. B) Confocal microscopy images representing the attachment of E. coli <t>BL21</t> control and E. coli BL21_BCAS0292 strains to CFBE41o-cells. Bacteria were labelled using a primary anti- E. coli FITC-conjugated antibody (green). Nuclei of CFBE41o-cells were counterstained with DAPI (blue) and actin stained with phalloidin conjugated with Alexa fluor 568. C) Attachment E. coli BL21 control and E. coli BL21_BCAS0292 to CFBE41o-cells, using an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain, with 20 fields of view counted per data point in three independent experiments. D) Confirmation of expression of BCAS0292, following induction in E. coli BL21 cells with 1mM IPTG by Coomassie Blue stained SDS PAGE gel (conditions used for Figure 1C ). Lane 1: MW marker; Lane 2: E. coli BL21 expression control; Lane 3: E. coli BL21_BCAS0292 after incubation with IPTG (1mM) for 2 h.
    Bl21 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 cells/product/Thermo Fisher
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    bl21 cells - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher e coli bl21 cells
    Spot assays used for monitoring the growth performance of <t>BL21/pEZYHb</t> and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress
    E Coli Bl21 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 cells/product/Thermo Fisher
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 cells - by Bioz Stars, 2020-09
    99/100 stars
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    84
    Thermo Fisher purification competent bl21 star de3 e coli cells
    Spot assays used for monitoring the growth performance of <t>BL21/pEZYHb</t> and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress
    Purification Competent Bl21 Star De3 E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/purification competent bl21 star de3 e coli cells/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    purification competent bl21 star de3 e coli cells - by Bioz Stars, 2020-09
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    Image Search Results


    Role of BCAS0292 in epithelial cell attachment. A) Attachment of K56-2 WT, ΔBCAS0292 mutant and Δ0292_0292 complemented strain to CFBE41o − cells at an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain with 20 fields of view counted per data point in three independent experiments, ** p =0.0073. B) Confocal microscopy images representing the attachment of E. coli BL21 control and E. coli BL21_BCAS0292 strains to CFBE41o-cells. Bacteria were labelled using a primary anti- E. coli FITC-conjugated antibody (green). Nuclei of CFBE41o-cells were counterstained with DAPI (blue) and actin stained with phalloidin conjugated with Alexa fluor 568. C) Attachment E. coli BL21 control and E. coli BL21_BCAS0292 to CFBE41o-cells, using an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain, with 20 fields of view counted per data point in three independent experiments. D) Confirmation of expression of BCAS0292, following induction in E. coli BL21 cells with 1mM IPTG by Coomassie Blue stained SDS PAGE gel (conditions used for Figure 1C ). Lane 1: MW marker; Lane 2: E. coli BL21 expression control; Lane 3: E. coli BL21_BCAS0292 after incubation with IPTG (1mM) for 2 h.

    Journal: bioRxiv

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia

    doi: 10.1101/2020.08.06.226779

    Figure Lengend Snippet: Role of BCAS0292 in epithelial cell attachment. A) Attachment of K56-2 WT, ΔBCAS0292 mutant and Δ0292_0292 complemented strain to CFBE41o − cells at an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain with 20 fields of view counted per data point in three independent experiments, ** p =0.0073. B) Confocal microscopy images representing the attachment of E. coli BL21 control and E. coli BL21_BCAS0292 strains to CFBE41o-cells. Bacteria were labelled using a primary anti- E. coli FITC-conjugated antibody (green). Nuclei of CFBE41o-cells were counterstained with DAPI (blue) and actin stained with phalloidin conjugated with Alexa fluor 568. C) Attachment E. coli BL21 control and E. coli BL21_BCAS0292 to CFBE41o-cells, using an MOI 50:1. Data represents the mean number of bacteria/ 100 cells for each strain, with 20 fields of view counted per data point in three independent experiments. D) Confirmation of expression of BCAS0292, following induction in E. coli BL21 cells with 1mM IPTG by Coomassie Blue stained SDS PAGE gel (conditions used for Figure 1C ). Lane 1: MW marker; Lane 2: E. coli BL21 expression control; Lane 3: E. coli BL21_BCAS0292 after incubation with IPTG (1mM) for 2 h.

    Article Snippet: Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C.

    Techniques: Cell Attachment Assay, Mutagenesis, Confocal Microscopy, Staining, Expressing, SDS Page, Marker, Incubation

    Spot assays used for monitoring the growth performance of BL21/pEZYHb and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress

    Journal: BMC Genomics

    Article Title: The alcohol dehydrogenase gene family in sugarcane and its involvement in cold stress regulation

    doi: 10.1186/s12864-020-06929-9

    Figure Lengend Snippet: Spot assays used for monitoring the growth performance of BL21/pEZYHb and BL21/pEZYHb- ScADH3 transformed E. coli cells on LB plates under cold stress. The growth performance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells on LB plates without supplements or stresses were used as controls. After spotting the sample on LB agar plates, incubation at 4 °C in darkness for 3 days, 7 days, and 14 days was used to study the tolerance of BL21/pEZYHb and BL21/ pEZYHb- ScADH3 cells under cold stress

    Article Snippet: When E. coli BL21 cells containing different vectors were grown to OD600 of 0.6, 1.0 mmol L− 1 isopropyl β-D-thiogalactoside (IPTG) was added to induce protein production.

    Techniques: Transformation Assay, Incubation