bl21 de3 strain  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 de3 strain
    Bl21 De3 Strain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 strain/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 strain - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: .. Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The primers OTBS1837-31-F (5′-GTGGATCCATGGTATTACCTCAACAAAAATC-3′) and OTBS1837-466-R (5′-GTCTCGAGTTACTTATTAATATTAGGTAAAGC-3′) were used for amplification of a truncated form (amino acids 31–466) of the 47-kDa OMP gene (GenBank accession number NC_009488.1).

    Article Title: Redesign of substrate specificity and identification of aminoglycoside binding residues of Eis from Mycobacterium tuberculosis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21(DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. The pET28a plasmid used for cloning experiments was purchased from Novagen (Gibbstown, NJ).

    Article Title: In Silico, In Vitro and In Vivo Analysis of Binding Affinity between N and C-Domains of Clostridium perfringens Alpha Toxin
    Article Snippet: E. coli DH5α and BL21 DE3 strains and pRSET A plasmid were procured from Invitrogen, Bengaluru, India. .. C. perfringens culture was maintained in Fluid Thioglycollate broth and incubated in anaerobic jars (Himedia) at 37° C. Luria Bertani broth with appropriate amount of ampicillin antibiotic (Sigma, India) was used in all cloning and expression experiments.

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: The plastidal Protox C-terminal region derived from Ser-303 to Lys-562 (Fig. ) was cloned in-frame in the pET-28(a)+ vector (Novagen, Madison, WI). .. The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech).

    Article Title: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm
    Article Snippet: .. DNA manipulation, bacterial strains, growth media and chemicals E. coli strain DH5α was used for standard cloning procedures while expression was carried out in the BL21 (DE3) strain (Invitrogen, US). .. The synthetic Burkholderia cepacia lipase (BCL) gene was designed based on the protein sequence corresponding to the 3LIP crystal structure [ ], codon optimized for expression in E. coli and synthesized at Genscript (US).

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. Bacteria clones were cultured in the LB (Luria broth) with kanamycin in an incubator shaker at 37 °C and 250 rpm overnight (New Brunswick).

    Amplification:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The primers OTBS1837-31-F (5′-GTGGATCCATGGTATTACCTCAACAAAAATC-3′) and OTBS1837-466-R (5′-GTCTCGAGTTACTTATTAATATTAGGTAAAGC-3′) were used for amplification of a truncated form (amino acids 31–466) of the 47-kDa OMP gene (GenBank accession number NC_009488.1).

    Synthesized:

    Article Title: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm
    Article Snippet: DNA manipulation, bacterial strains, growth media and chemicals E. coli strain DH5α was used for standard cloning procedures while expression was carried out in the BL21 (DE3) strain (Invitrogen, US). .. The synthetic Burkholderia cepacia lipase (BCL) gene was designed based on the protein sequence corresponding to the 3LIP crystal structure [ ], codon optimized for expression in E. coli and synthesized at Genscript (US).

    Construct:

    Article Title: Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus
    Article Snippet: The BL21(DE3) strain of E . coli (Invitrogen) was used as an expression system for recombinant pET16b encoding 10 x His-BnaDGAT11-113 or 6 x His-BnaDGAT11-80 . .. Genetic constructs were designed such that each recombinant polypeptide segment contained an N-terminal tag as shown in the supplemental data.

    Incubation:

    Article Title: In Silico, In Vitro and In Vivo Analysis of Binding Affinity between N and C-Domains of Clostridium perfringens Alpha Toxin
    Article Snippet: E. coli DH5α and BL21 DE3 strains and pRSET A plasmid were procured from Invitrogen, Bengaluru, India. .. C. perfringens culture was maintained in Fluid Thioglycollate broth and incubated in anaerobic jars (Himedia) at 37° C. Luria Bertani broth with appropriate amount of ampicillin antibiotic (Sigma, India) was used in all cloning and expression experiments.

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen). .. The pellet was resuspended on ice in lysis buffer (phosphate-buffered saline supplemented with complete protease inhibitors (EDTA-free, Roche), phenylmethyl sulfonyl fluoride (Sigma) and 150 μM lysozyme (Sigma) and incubated on ice for 30 min. Triton-X 100 (1%), MgCl2 (10mM) and DNase I (5 μg/ml, Roche) were added, and the lysate was incubated on ice for 30 min.

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. The next day, 50 μL medium containing bacteria was put into 50 ml fresh LB with kanamycin and incubated again; when the OD (Optical Density) reached 0.6, IPTG (Isopropyl β-D-1- thiogalactopy- ranoside) was added to the medium at a final concentration of 0.1 mM.

    Infection:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: At 3–4 days postinfection, infectivity was determined using an indirect immunofluorescence assay. .. Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively.

    Expressing:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: .. Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The primers OTBS1837-31-F (5′-GTGGATCCATGGTATTACCTCAACAAAAATC-3′) and OTBS1837-466-R (5′-GTCTCGAGTTACTTATTAATATTAGGTAAAGC-3′) were used for amplification of a truncated form (amino acids 31–466) of the 47-kDa OMP gene (GenBank accession number NC_009488.1).

    Article Title: In Silico, In Vitro and In Vivo Analysis of Binding Affinity between N and C-Domains of Clostridium perfringens Alpha Toxin
    Article Snippet: E. coli DH5α and BL21 DE3 strains and pRSET A plasmid were procured from Invitrogen, Bengaluru, India. .. C. perfringens culture was maintained in Fluid Thioglycollate broth and incubated in anaerobic jars (Himedia) at 37° C. Luria Bertani broth with appropriate amount of ampicillin antibiotic (Sigma, India) was used in all cloning and expression experiments.

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: .. The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen). .. Bacteria were grown in Luria-Bertani medium to an OD of 0.6, and the expression of the fusion protein was induced with 0,5mM isopropyl-1-thio-β-D-galactopyranoside (AppliChem).

    Article Title: Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus
    Article Snippet: .. The BL21(DE3) strain of E . coli (Invitrogen) was used as an expression system for recombinant pET16b encoding 10 x His-BnaDGAT11-113 or 6 x His-BnaDGAT11-80 . .. Genetic constructs were designed such that each recombinant polypeptide segment contained an N-terminal tag as shown in the supplemental data.

    Article Title: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm
    Article Snippet: .. DNA manipulation, bacterial strains, growth media and chemicals E. coli strain DH5α was used for standard cloning procedures while expression was carried out in the BL21 (DE3) strain (Invitrogen, US). .. The synthetic Burkholderia cepacia lipase (BCL) gene was designed based on the protein sequence corresponding to the 3LIP crystal structure [ ], codon optimized for expression in E. coli and synthesized at Genscript (US).

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: .. Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. Bacteria clones were cultured in the LB (Luria broth) with kanamycin in an incubator shaker at 37 °C and 250 rpm overnight (New Brunswick).

    Article Title: Atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain in complex with the LDL-receptor tail
    Article Snippet: The PTB domain (residues 43–174) of rARH was overexpressed in Esherichia coli , BL21 DE3 strain (Invitrogen), and purified initially by Ni+2 affinity. .. Expression of rARH yielded 5–10 mg/L of 99% pure protein, whereas the PTB domain of rARH yielded 20–30 mg/L, and crystallized only in the presence of the LDLR peptide.

    Modification:

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: The generation of the GST fusion FT-PrP expression vector (pGEX-KG FT-PrP) was described previously; a modified purification protocol was used . .. The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen).

    Transformation Assay:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The plasmid containing recombinant 47-kDa OMP (rec47) was transformed into E. coli BL21 (DE3) for overexpression.

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: .. The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen). .. Bacteria were grown in Luria-Bertani medium to an OD of 0.6, and the expression of the fusion protein was induced with 0,5mM isopropyl-1-thio-β-D-galactopyranoside (AppliChem).

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: .. Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. Bacteria clones were cultured in the LB (Luria broth) with kanamycin in an incubator shaker at 37 °C and 250 rpm overnight (New Brunswick).

    Over Expression:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The plasmid containing recombinant 47-kDa OMP (rec47) was transformed into E. coli BL21 (DE3) for overexpression.

    Crystallization Assay:

    Article Title: Atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain in complex with the LDL-receptor tail
    Article Snippet: The PTB domain (residues 43–174) of rARH was overexpressed in Esherichia coli , BL21 DE3 strain (Invitrogen), and purified initially by Ni+2 affinity. .. Before crystallization the protein was mixed with 5- to 10-fold molar excess of the synthetic LDLR peptide (NSIN F D NP V Y QKTT) and concentrated to ≈4.7 mg/mL.

    Derivative Assay:

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: The plastidal Protox C-terminal region derived from Ser-303 to Lys-562 (Fig. ) was cloned in-frame in the pET-28(a)+ vector (Novagen, Madison, WI). .. The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech).

    Chromatography:

    Article Title: A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region ▿
    Article Snippet: .. For the affinity purification of antibodies, His6 -tagged proteins induced from pET-S1Δ, -S2, and -S3 in the BL21(DE3) strain of E. coli were purified by Ni-nitrilotriacetic acid chromatography (Invitrogen) and coupled to Affi-gel 10 resin (Bio-Rad). ..

    Cell Culture:

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. Bacteria clones were cultured in the LB (Luria broth) with kanamycin in an incubator shaker at 37 °C and 250 rpm overnight (New Brunswick).

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA). .. E. coli strains containing pET-26b vector were cultured at 37 °C in LB with final concentration 30 μg/ml of kanamycin.

    Polymerase Chain Reaction:

    Article Title: Biochemical and Structural Analysis of an Eis Family Aminoglycoside Acetyltransferase from Bacillus anthracis
    Article Snippet: The chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. PCR primers were purchased from Integrated DNA Technologies (IDT; Coralville, IA).

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The polymerase chain reaction product was digested with Bam HI and Xho I, and cloned into the bacterial expression vector pRSET A (Invitrogen).

    Article Title: Redesign of substrate specificity and identification of aminoglycoside binding residues of Eis from Mycobacterium tuberculosis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21(DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. PCR primers were purchased from Integrated DNA Technologies (Coralville, IA).

    Article Title: The aminoglycoside multi-acetylating activity of the enhanced intracellular survival (Eis) protein from Mycobacterium smegmatis and its inhibition
    Article Snippet: The chemically competent E. coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. MC2 155 used for PCR was a generous gift from Dr. Sabine Ehrt (Weill Cornell Medical College).

    Article Title: Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. Chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA).

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: DNA extraction kit was purchased from Peqlab and PCR product purification kit was obtained from BioNEER (Seoul, Korea). .. E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA).

    Affinity Purification:

    Article Title: A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region ▿
    Article Snippet: .. For the affinity purification of antibodies, His6 -tagged proteins induced from pET-S1Δ, -S2, and -S3 in the BL21(DE3) strain of E. coli were purified by Ni-nitrilotriacetic acid chromatography (Invitrogen) and coupled to Affi-gel 10 resin (Bio-Rad). ..

    Recombinant:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: Paragraph title: Bacterial strains and generation of recombinant plasmids. ... Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively.

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: Paragraph title: Purification of recombinant proteins ... The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen).

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech). .. The protein was further purified on a HiTrap SP column (5 mL; Amersham Pharmacia Biotech), and the purified recombinant protein (10 mg) was used to immunize rabbits.

    Article Title: Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus
    Article Snippet: .. The BL21(DE3) strain of E . coli (Invitrogen) was used as an expression system for recombinant pET16b encoding 10 x His-BnaDGAT11-113 or 6 x His-BnaDGAT11-80 . .. Genetic constructs were designed such that each recombinant polypeptide segment contained an N-terminal tag as shown in the supplemental data.

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: .. Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. Bacteria clones were cultured in the LB (Luria broth) with kanamycin in an incubator shaker at 37 °C and 250 rpm overnight (New Brunswick).

    Immunofluorescence:

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: At 3–4 days postinfection, infectivity was determined using an indirect immunofluorescence assay. .. Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively.

    DNA Extraction:

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: DNA extraction kit was purchased from Peqlab and PCR product purification kit was obtained from BioNEER (Seoul, Korea). .. E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA).

    Nucleic Acid Electrophoresis:

    Article Title: A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region ▿
    Article Snippet: β-Galactosidase fusion proteins were induced from pUR-S1, -S2, and -S3 in the TG1 strain of Escherichia coli by standard protocols, followed by 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ( ). .. For the affinity purification of antibodies, His6 -tagged proteins induced from pET-S1Δ, -S2, and -S3 in the BL21(DE3) strain of E. coli were purified by Ni-nitrilotriacetic acid chromatography (Invitrogen) and coupled to Affi-gel 10 resin (Bio-Rad).

    Isolation:

    Article Title: Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila
    Article Snippet: Bacterial strains and vector A. hydrophila (AH17) isolated from pond water was obtained from Dr. .. Escherichia coli DH5α and BL21 (DE3) strains were from GIBCO BRL, USA and Novagen, USA, respectively.

    Size-exclusion Chromatography:

    Article Title: Atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain in complex with the LDL-receptor tail
    Article Snippet: The PTB domain (residues 43–174) of rARH was overexpressed in Esherichia coli , BL21 DE3 strain (Invitrogen), and purified initially by Ni+2 affinity. .. Thrombin-digested products were further purified on a HiTrap SP column at pH 6.5, followed by size exclusion chromatography on a Superdex-200 column (GE Healthcare).

    Purification:

    Article Title: A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region ▿
    Article Snippet: .. For the affinity purification of antibodies, His6 -tagged proteins induced from pET-S1Δ, -S2, and -S3 in the BL21(DE3) strain of E. coli were purified by Ni-nitrilotriacetic acid chromatography (Invitrogen) and coupled to Affi-gel 10 resin (Bio-Rad). ..

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: Paragraph title: Purification of recombinant proteins ... The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen).

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: .. The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech). .. The protein was further purified on a HiTrap SP column (5 mL; Amersham Pharmacia Biotech), and the purified recombinant protein (10 mg) was used to immunize rabbits.

    Article Title: Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus
    Article Snippet: Paragraph title: Preparation and purification of BnaDGAT1 truncations ... The BL21(DE3) strain of E . coli (Invitrogen) was used as an expression system for recombinant pET16b encoding 10 x His-BnaDGAT11-113 or 6 x His-BnaDGAT11-80 .

    Article Title: Atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain in complex with the LDL-receptor tail
    Article Snippet: .. The PTB domain (residues 43–174) of rARH was overexpressed in Esherichia coli , BL21 DE3 strain (Invitrogen), and purified initially by Ni+2 affinity. .. Thrombin-digested products were further purified on a HiTrap SP column at pH 6.5, followed by size exclusion chromatography on a Superdex-200 column (GE Healthcare).

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: DNA extraction kit was purchased from Peqlab and PCR product purification kit was obtained from BioNEER (Seoul, Korea). .. E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA).

    Sequencing:

    Article Title: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm
    Article Snippet: DNA manipulation, bacterial strains, growth media and chemicals E. coli strain DH5α was used for standard cloning procedures while expression was carried out in the BL21 (DE3) strain (Invitrogen, US). .. The synthetic Burkholderia cepacia lipase (BCL) gene was designed based on the protein sequence corresponding to the 3LIP crystal structure [ ], codon optimized for expression in E. coli and synthesized at Genscript (US).

    Positron Emission Tomography:

    Article Title: A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region ▿
    Article Snippet: .. For the affinity purification of antibodies, His6 -tagged proteins induced from pET-S1Δ, -S2, and -S3 in the BL21(DE3) strain of E. coli were purified by Ni-nitrilotriacetic acid chromatography (Invitrogen) and coupled to Affi-gel 10 resin (Bio-Rad). ..

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: The plastidal Protox C-terminal region derived from Ser-303 to Lys-562 (Fig. ) was cloned in-frame in the pET-28(a)+ vector (Novagen, Madison, WI). .. The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech).

    Article Title: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm
    Article Snippet: DNA manipulation, bacterial strains, growth media and chemicals E. coli strain DH5α was used for standard cloning procedures while expression was carried out in the BL21 (DE3) strain (Invitrogen, US). .. Both BCL and the green fluorescent protein (GFP) were expressed from the pET-19b plasmid (Novagen, US).

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: .. E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA). .. Cultivation of Cohnella sp. A01, was carried out at 55 °C in LB medium for 4 days and it΄s genomic DNA was extracted using the high pure DNA extraction kit.

    IA:

    Article Title: Biochemical and Structural Analysis of an Eis Family Aminoglycoside Acetyltransferase from Bacillus anthracis
    Article Snippet: The chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. PCR primers were purchased from Integrated DNA Technologies (IDT; Coralville, IA).

    Article Title: Redesign of substrate specificity and identification of aminoglycoside binding residues of Eis from Mycobacterium tuberculosis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21(DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. PCR primers were purchased from Integrated DNA Technologies (Coralville, IA).

    Article Title: Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. Chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA).

    SDS Page:

    Article Title: A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region ▿
    Article Snippet: β-Galactosidase fusion proteins were induced from pUR-S1, -S2, and -S3 in the TG1 strain of Escherichia coli by standard protocols, followed by 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ( ). .. For the affinity purification of antibodies, His6 -tagged proteins induced from pET-S1Δ, -S2, and -S3 in the BL21(DE3) strain of E. coli were purified by Ni-nitrilotriacetic acid chromatography (Invitrogen) and coupled to Affi-gel 10 resin (Bio-Rad).

    Plasmid Preparation:

    Article Title: Biochemical and Structural Analysis of an Eis Family Aminoglycoside Acetyltransferase from Bacillus anthracis
    Article Snippet: The chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. The pET15b vector was purchased from Novagen (Gibbstown, NJ).

    Article Title: A Recombinant 47-kDa Outer Membrane Protein Induces an Immune Response against Orientia tsutsugamushi Strain Boryong
    Article Snippet: Escherichia coli TOP10 and BL21 DE3 strains (Invitrogen, Carlsbad, CA) were used for cloning and prokaryotic expression, respectively. .. The polymerase chain reaction product was digested with Bam HI and Xho I, and cloned into the bacterial expression vector pRSET A (Invitrogen).

    Article Title: Redesign of substrate specificity and identification of aminoglycoside binding residues of Eis from Mycobacterium tuberculosis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21(DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. The pET28a plasmid used for cloning experiments was purchased from Novagen (Gibbstown, NJ).

    Article Title: The aminoglycoside multi-acetylating activity of the enhanced intracellular survival (Eis) protein from Mycobacterium smegmatis and its inhibition
    Article Snippet: The chemically competent E. coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. The pET28a plasmid was purchased from Novagen (Gibbstown, NJ).

    Article Title: In Silico, In Vitro and In Vivo Analysis of Binding Affinity between N and C-Domains of Clostridium perfringens Alpha Toxin
    Article Snippet: .. E. coli DH5α and BL21 DE3 strains and pRSET A plasmid were procured from Invitrogen, Bengaluru, India. ..

    Article Title: Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila
    Article Snippet: Paragraph title: Bacterial strains and vector ... Escherichia coli DH5α and BL21 (DE3) strains were from GIBCO BRL, USA and Novagen, USA, respectively.

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: .. The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen). .. Bacteria were grown in Luria-Bertani medium to an OD of 0.6, and the expression of the fusion protein was induced with 0,5mM isopropyl-1-thio-β-D-galactopyranoside (AppliChem).

    Article Title: Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis
    Article Snippet: Chemically competent Escherichia coli TOP10 and BL21 (DE3) strains were purchased from Invitrogen (Carlsbad, CA). .. The pET28a plasmid used in this study was purchased from Novagen (Gibbstown, NJ).

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: The plastidal Protox C-terminal region derived from Ser-303 to Lys-562 (Fig. ) was cloned in-frame in the pET-28(a)+ vector (Novagen, Madison, WI). .. The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech).

    Article Title: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm
    Article Snippet: DNA manipulation, bacterial strains, growth media and chemicals E. coli strain DH5α was used for standard cloning procedures while expression was carried out in the BL21 (DE3) strain (Invitrogen, US). .. Both BCL and the green fluorescent protein (GFP) were expressed from the pET-19b plasmid (Novagen, US).

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: .. E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA). .. Cultivation of Cohnella sp. A01, was carried out at 55 °C in LB medium for 4 days and it΄s genomic DNA was extracted using the high pure DNA extraction kit.

    Affinity Chromatography:

    Article Title: Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts 1
    Article Snippet: The resulting His-tag fusion protein was overproduced in the BL21(DE3) strain of Escherichia coli (Invitrogen) and purified using a HisTrap kit according to the manufacturer's protocol (Amersham Pharmacia Biotech). .. Anti-serum was further purified by affinity chromatography with a HiTrap NHS-activated Sepharose column (Amersham Pharmacia Biotech).

    Concentration Assay:

    Article Title: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration
    Article Snippet: Prokaryotic expression for recombinant protein Plasmids for KGA and GAC deletion mutants were transformed into the BL21 (DE3) strain (Invitrogen, Carlsbad, CA) individually for prokaryotic expression. .. The next day, 50 μL medium containing bacteria was put into 50 ml fresh LB with kanamycin and incubated again; when the OD (Optical Density) reached 0.6, IPTG (Isopropyl β-D-1- thiogalactopy- ranoside) was added to the medium at a final concentration of 0.1 mM.

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA). .. E. coli strains containing pET-26b vector were cultured at 37 °C in LB with final concentration 30 μg/ml of kanamycin.

    Marker:

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization
    Article Snippet: Protein molecular mass marker, IPTG, amino acids, DTT, BME, GSH, ascorbic acid, iodoacetate, iodoacetamide were acquired from Merck. .. E. coli DH5α and BL21 (DE3) strains and pET-26b vector resistant to kanamycin were purchased from Invitrogen (Carlsbad, USA).

    Lysis:

    Article Title: The prion protein is an agonistic ligand of the G protein-coupled receptor Gpr126/Adgrg6
    Article Snippet: The FT-PrP expression vector was transformed into BL21 (DE3) strain of Escherichia coli (Invitrogen). .. The pellet was resuspended on ice in lysis buffer (phosphate-buffered saline supplemented with complete protease inhibitors (EDTA-free, Roche), phenylmethyl sulfonyl fluoride (Sigma) and 150 μM lysozyme (Sigma) and incubated on ice for 30 min. Triton-X 100 (1%), MgCl2 (10mM) and DNase I (5 μg/ml, Roche) were added, and the lysate was incubated on ice for 30 min.

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    Thermo Fisher e coli strain bl21
    Survival of Escherichia coli cells expressing the fusion protein GST-NdCTR1 or GST depends on the time of treatment with silver nanoparticles. Notes: ( A ) Protocols of the experiments demonstrating that NdCTR1 rescued bacteria treated with the SNPs. ( B and C ) Histograms showing survival of E. coli cells expressing GST (black) or GST-NdCTR1 (gray) after treatment with 10 μM SNPs and 100 μM SNPs. Abbreviations: SNPs, silver nanoparticles; NdCTR1, N-terminal domain of CTR1; GST-NdCTR1, glutathione-S-transferase-N-terminal domain of CTR1; <t>BL21</t> (DE3)/pGEX-4T-1, E. coli strain synthetizing GST; BL21 (DE3)/pNdCTR1, E. coli strain synthetizing GST-NdCTR1; CFU, colony-forming unit.
    E Coli Strain Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain bl21/product/Thermo Fisher
    Average 99 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    e coli strain bl21 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher e coli bl21
    Survival of Escherichia coli cells expressing the fusion protein GST-NdCTR1 or GST depends on the time of treatment with silver nanoparticles. Notes: ( A ) Protocols of the experiments demonstrating that NdCTR1 rescued bacteria treated with the SNPs. ( B and C ) Histograms showing survival of E. coli cells expressing GST (black) or GST-NdCTR1 (gray) after treatment with 10 μM SNPs and 100 μM SNPs. Abbreviations: SNPs, silver nanoparticles; NdCTR1, N-terminal domain of CTR1; GST-NdCTR1, glutathione-S-transferase-N-terminal domain of CTR1; <t>BL21</t> (DE3)/pGEX-4T-1, E. coli strain synthetizing GST; BL21 (DE3)/pNdCTR1, E. coli strain synthetizing GST-NdCTR1; CFU, colony-forming unit.
    E Coli Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

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    Survival of Escherichia coli cells expressing the fusion protein GST-NdCTR1 or GST depends on the time of treatment with silver nanoparticles. Notes: ( A ) Protocols of the experiments demonstrating that NdCTR1 rescued bacteria treated with the SNPs. ( B and C ) Histograms showing survival of E. coli cells expressing GST (black) or GST-NdCTR1 (gray) after treatment with 10 μM SNPs and 100 μM SNPs. Abbreviations: SNPs, silver nanoparticles; NdCTR1, N-terminal domain of CTR1; GST-NdCTR1, glutathione-S-transferase-N-terminal domain of CTR1; BL21 (DE3)/pGEX-4T-1, E. coli strain synthetizing GST; BL21 (DE3)/pNdCTR1, E. coli strain synthetizing GST-NdCTR1; CFU, colony-forming unit.

    Journal: International Journal of Nanomedicine

    Article Title: New silver nanoparticles induce apoptosis-like process in E. coli and interfere with mammalian copper metabolism

    doi: 10.2147/IJN.S117745

    Figure Lengend Snippet: Survival of Escherichia coli cells expressing the fusion protein GST-NdCTR1 or GST depends on the time of treatment with silver nanoparticles. Notes: ( A ) Protocols of the experiments demonstrating that NdCTR1 rescued bacteria treated with the SNPs. ( B and C ) Histograms showing survival of E. coli cells expressing GST (black) or GST-NdCTR1 (gray) after treatment with 10 μM SNPs and 100 μM SNPs. Abbreviations: SNPs, silver nanoparticles; NdCTR1, N-terminal domain of CTR1; GST-NdCTR1, glutathione-S-transferase-N-terminal domain of CTR1; BL21 (DE3)/pGEX-4T-1, E. coli strain synthetizing GST; BL21 (DE3)/pNdCTR1, E. coli strain synthetizing GST-NdCTR1; CFU, colony-forming unit.

    Article Snippet: E. coli strain BL21 (DE3)/pNdCTR1 was obtained by chemical transformation (TransformAid™; Thermo Scientific) of bacteria.

    Techniques: Expressing