bl21 de3 competent e coli  (New England Biolabs)


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    Name:
    BL21 DE3 Competent E coli
    Description:
    BL21 DE3 Competent E coli 20x0 05 ml
    Catalog Number:
    c2527h
    Price:
    204
    Size:
    1 ml
    Category:
    Competent Bacteria
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    New England Biolabs bl21 de3 competent e coli
    BL21 DE3 Competent E coli
    BL21 DE3 Competent E coli 20x0 05 ml
    https://www.bioz.com/result/bl21 de3 competent e coli/product/New England Biolabs
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 competent e coli - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Engineering of CRISPR-Cas12b for human genome editing
    Article Snippet: .. Cas12b genes were cloned into bacterial expression plasmids (T7-TwinStrep-SUMO-NLS-Cas12b-NLS-3xHA) and expressed in BL21(DE3) cells (NEB #C2527H) containing a pLysS-tRNA plasmid (from Novagen #70956). ..

    Article Title: Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis
    Article Snippet: .. Bacterial strains and culture conditions Standard cloning and metabolic engineering were carried out using E. coli strains 10β and BL21 (DE3), respectively (both supplied by New England Biolabs, Ipswich, MA, USA). .. For general cloning, the bacteria were cultivated in lysogenic broth (LB) medium, whereas for all other experiments the bacteria were cultivated in M9 medium supplemented with 0.5% (w/v) glucose and appropriate antibiotics (50 μg mL−1 kanamycin, 100 μg mL−1 ampicillin and/or 25 μg mL−1 chloramphenicol) at 37 °C [ ].

    Cell Culture:

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Expression of the SID protein in bacteria After verifying the sequence of the pCold-sid clone, the vector was transformed into BL21 (DE3) pLysS competent cells (NEB, Ipswich, MA) and a single colony was cultured in LB containing 100 µg/ml ampicillin at 37°C overnight. ..

    other:

    Article Title: The traffic ATPase PilF interacts with the inner membrane platform of the DNA translocator and type IV pili from Thermus thermophilus
    Article Snippet: Organisms and cultivation Escherichia coli DH5α, BL21 (DE3) and T7 Express cells harboring pET28a plasmids (New England Biolabs, Ipswich, MA, USA) were grown at 37 °C in LB medium or 2 × YT medium containing 20 μg·mL−1 kanamycin.

    Expressing:

    Article Title: Engineering of CRISPR-Cas12b for human genome editing
    Article Snippet: .. Cas12b genes were cloned into bacterial expression plasmids (T7-TwinStrep-SUMO-NLS-Cas12b-NLS-3xHA) and expressed in BL21(DE3) cells (NEB #C2527H) containing a pLysS-tRNA plasmid (from Novagen #70956). ..

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Expression of the SID protein in bacteria After verifying the sequence of the pCold-sid clone, the vector was transformed into BL21 (DE3) pLysS competent cells (NEB, Ipswich, MA) and a single colony was cultured in LB containing 100 µg/ml ampicillin at 37°C overnight. ..

    Sequencing:

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Expression of the SID protein in bacteria After verifying the sequence of the pCold-sid clone, the vector was transformed into BL21 (DE3) pLysS competent cells (NEB, Ipswich, MA) and a single colony was cultured in LB containing 100 µg/ml ampicillin at 37°C overnight. ..

    Transformation Assay:

    Article Title: The Biochemical Basis of Vitamin A Production from the Asymmetric Carotenoid β-Cryptoxanthin
    Article Snippet: .. The plasmids were transformed into BL21 (DE3) competent E. coli (New England BioLabs) using a standard protocol. .. Transformed bacterial cells were grown in Luria Bertani broth at 37°C with constant shaking until reaching optical density of A600 =0.6.

    Article Title: Identification of the agr Peptide of Listeria monocytogenes
    Article Snippet: .. AIP Production in E. coli For heterologous AIP production, pET29a_agrB or pET29a_agrBD were transformed into E. coli BL21(DE3) (New England Biolabs) and transformants were selected on LB agar containing kanamycin. ..

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Expression of the SID protein in bacteria After verifying the sequence of the pCold-sid clone, the vector was transformed into BL21 (DE3) pLysS competent cells (NEB, Ipswich, MA) and a single colony was cultured in LB containing 100 µg/ml ampicillin at 37°C overnight. ..

    Plasmid Preparation:

    Article Title: Engineering of CRISPR-Cas12b for human genome editing
    Article Snippet: .. Cas12b genes were cloned into bacterial expression plasmids (T7-TwinStrep-SUMO-NLS-Cas12b-NLS-3xHA) and expressed in BL21(DE3) cells (NEB #C2527H) containing a pLysS-tRNA plasmid (from Novagen #70956). ..

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Expression of the SID protein in bacteria After verifying the sequence of the pCold-sid clone, the vector was transformed into BL21 (DE3) pLysS competent cells (NEB, Ipswich, MA) and a single colony was cultured in LB containing 100 µg/ml ampicillin at 37°C overnight. ..

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  • 99
    New England Biolabs bl21 de3 competent e coli
    Bl21 De3 Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 competent e coli/product/New England Biolabs
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 competent e coli - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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