bl21 bacterial cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 bacterial cells
    Bl21 Bacterial Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 bacterial cells/product/Thermo Fisher
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 bacterial cells - by Bioz Stars, 2020-02
    79/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: cDNA-encoding residues 475–568 of AF9 was cloned into pFLAG-MAC (Sigma) to generate pFLAG-AF9-ct. .. This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression.

    Centrifugation:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. Cells were lysed using a French Press and debris was removed by centrifugation at 10,000 × g for 20 min.

    In Vitro:

    Article Title: MDM2 Inhibits Axin-Induced p53 Activation Independently of its E3 Ligase Activity
    Article Snippet: .. In vitro Binding Assay The proteins His-Axin, His-p53, GST-MDM2, GST-MDM2 (C464A) and GST- MDM2Δp53 were expressed in BL21 bacterial cells (purchased from Invitrogen) induced by 1 mM IPTG for 6 h at 26°C, then were purified using His-select nickel affinity gel (Sigma) or glutathione-agarose beads (GE). ..

    Article Title: Regulation of Adipocyte Differentiation by the Zinc Finger Protein ZNF638 *
    Article Snippet: GST Pulldown Assays GST-ZNF638 fragments were purified from BL21 bacterial cells (Invitrogen) according to the following protocol. .. In vitro translated and radiolabeled C/EBPs were generated (Promega) with EasyTag EXPRESS35 S protein labeling mix (PerkinElmer Life Sciences).

    Incubation:

    Article Title: MDM2 Inhibits Axin-Induced p53 Activation Independently of its E3 Ligase Activity
    Article Snippet: In vitro Binding Assay The proteins His-Axin, His-p53, GST-MDM2, GST-MDM2 (C464A) and GST- MDM2Δp53 were expressed in BL21 bacterial cells (purchased from Invitrogen) induced by 1 mM IPTG for 6 h at 26°C, then were purified using His-select nickel affinity gel (Sigma) or glutathione-agarose beads (GE). .. Mixed proteins were incubated with rabbit anti-p53 antibody bound to protein A/G beads in lysis buffer for 3 h at 4°C .

    Article Title: Regulation of Adipocyte Differentiation by the Zinc Finger Protein ZNF638 *
    Article Snippet: GST Pulldown Assays GST-ZNF638 fragments were purified from BL21 bacterial cells (Invitrogen) according to the following protocol. .. Lysates were incubated with glutathione-Sepharose 4B beads (GE Healthcare) for 2 h at 4 °C and subsequently washed three times with lysis buffer.

    Construct:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: .. This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. Following induction of protein expression with isopropyl β- d -1-thiogalactopyranoside (IPTG), cell pellets were collected and resuspended in bacterial lysis buffer (20 m m Tris, pH 7.4, 1 m NaCl, 1 m m EDTA, and 1 m m dithiothreitol).

    Purification:

    Article Title: MDM2 Inhibits Axin-Induced p53 Activation Independently of its E3 Ligase Activity
    Article Snippet: .. In vitro Binding Assay The proteins His-Axin, His-p53, GST-MDM2, GST-MDM2 (C464A) and GST- MDM2Δp53 were expressed in BL21 bacterial cells (purchased from Invitrogen) induced by 1 mM IPTG for 6 h at 26°C, then were purified using His-select nickel affinity gel (Sigma) or glutathione-agarose beads (GE). ..

    Article Title: Regulation of Adipocyte Differentiation by the Zinc Finger Protein ZNF638 *
    Article Snippet: .. GST Pulldown Assays GST-ZNF638 fragments were purified from BL21 bacterial cells (Invitrogen) according to the following protocol. .. Cells were collected, pelleted, and lysed in 2 ml of lysis buffer (20 mm Tris (pH 8), 400 mm KCl, 1 mm EDTA, 0.1% Triton X-100, and 1 mm DTT).

    Protein Purification:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: Paragraph title: Protein Purification ... This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression.

    Concentration Assay:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. The supernatant was collected and diluted 1:5 with the lysis buffer containing no NaCl (final concentration of NaCl, 200 m m ).

    Generated:

    Article Title: Regulation of Adipocyte Differentiation by the Zinc Finger Protein ZNF638 *
    Article Snippet: GST Pulldown Assays GST-ZNF638 fragments were purified from BL21 bacterial cells (Invitrogen) according to the following protocol. .. In vitro translated and radiolabeled C/EBPs were generated (Promega) with EasyTag EXPRESS35 S protein labeling mix (PerkinElmer Life Sciences).

    Labeling:

    Article Title: Regulation of Adipocyte Differentiation by the Zinc Finger Protein ZNF638 *
    Article Snippet: GST Pulldown Assays GST-ZNF638 fragments were purified from BL21 bacterial cells (Invitrogen) according to the following protocol. .. In vitro translated and radiolabeled C/EBPs were generated (Promega) with EasyTag EXPRESS35 S protein labeling mix (PerkinElmer Life Sciences).

    Expressing:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: .. This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. Following induction of protein expression with isopropyl β- d -1-thiogalactopyranoside (IPTG), cell pellets were collected and resuspended in bacterial lysis buffer (20 m m Tris, pH 7.4, 1 m NaCl, 1 m m EDTA, and 1 m m dithiothreitol).

    Lysis:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. Following induction of protein expression with isopropyl β- d -1-thiogalactopyranoside (IPTG), cell pellets were collected and resuspended in bacterial lysis buffer (20 m m Tris, pH 7.4, 1 m NaCl, 1 m m EDTA, and 1 m m dithiothreitol).

    Article Title: MDM2 Inhibits Axin-Induced p53 Activation Independently of its E3 Ligase Activity
    Article Snippet: In vitro Binding Assay The proteins His-Axin, His-p53, GST-MDM2, GST-MDM2 (C464A) and GST- MDM2Δp53 were expressed in BL21 bacterial cells (purchased from Invitrogen) induced by 1 mM IPTG for 6 h at 26°C, then were purified using His-select nickel affinity gel (Sigma) or glutathione-agarose beads (GE). .. Mixed proteins were incubated with rabbit anti-p53 antibody bound to protein A/G beads in lysis buffer for 3 h at 4°C .

    Article Title: Regulation of Adipocyte Differentiation by the Zinc Finger Protein ZNF638 *
    Article Snippet: GST Pulldown Assays GST-ZNF638 fragments were purified from BL21 bacterial cells (Invitrogen) according to the following protocol. .. Cells were collected, pelleted, and lysed in 2 ml of lysis buffer (20 mm Tris (pH 8), 400 mm KCl, 1 mm EDTA, 0.1% Triton X-100, and 1 mm DTT).

    Western Blot:

    Article Title: MDM2 Inhibits Axin-Induced p53 Activation Independently of its E3 Ligase Activity
    Article Snippet: In vitro Binding Assay The proteins His-Axin, His-p53, GST-MDM2, GST-MDM2 (C464A) and GST- MDM2Δp53 were expressed in BL21 bacterial cells (purchased from Invitrogen) induced by 1 mM IPTG for 6 h at 26°C, then were purified using His-select nickel affinity gel (Sigma) or glutathione-agarose beads (GE). .. Precipitated proteins were washed by lysis buffer for 3 times and detected by western blotting using the appropriate antibodies.

    Transformation Assay:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: .. This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. Following induction of protein expression with isopropyl β- d -1-thiogalactopyranoside (IPTG), cell pellets were collected and resuspended in bacterial lysis buffer (20 m m Tris, pH 7.4, 1 m NaCl, 1 m m EDTA, and 1 m m dithiothreitol).

    Binding Assay:

    Article Title: MDM2 Inhibits Axin-Induced p53 Activation Independently of its E3 Ligase Activity
    Article Snippet: .. In vitro Binding Assay The proteins His-Axin, His-p53, GST-MDM2, GST-MDM2 (C464A) and GST- MDM2Δp53 were expressed in BL21 bacterial cells (purchased from Invitrogen) induced by 1 mM IPTG for 6 h at 26°C, then were purified using His-select nickel affinity gel (Sigma) or glutathione-agarose beads (GE). ..

    Plasmid Preparation:

    Article Title: Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression *
    Article Snippet: .. This plasmid construct was transformed into BL21 bacterial cells (Invitrogen) for protein expression. .. Following induction of protein expression with isopropyl β- d -1-thiogalactopyranoside (IPTG), cell pellets were collected and resuspended in bacterial lysis buffer (20 m m Tris, pH 7.4, 1 m NaCl, 1 m m EDTA, and 1 m m dithiothreitol).

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  • 90
    Thermo Fisher one shot bl21 de3 chemically competent e coli
    One Shot Bl21 De3 Chemically Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot bl21 de3 chemically competent e coli/product/Thermo Fisher
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    one shot bl21 de3 chemically competent e coli - by Bioz Stars, 2020-02
    90/100 stars
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    Thermo Fisher bl21 star de3 plyss one shot chemically competent e coli
    Bl21 Star De3 Plyss One Shot Chemically Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 star de3 plyss one shot chemically competent e coli/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bl21 star de3 plyss one shot chemically competent e coli - by Bioz Stars, 2020-02
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