bk ca  (Alomone Labs)


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    Alomone Labs bk ca
    Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bk ca - by Bioz Stars, 2023-01
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    bk ca  (Alomone Labs)


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    Alomone Labs bk ca
    Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bk ca/product/Alomone Labs
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    alomone apc  (Alomone Labs)


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    Alomone Labs alomone apc
    Alomone Apc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal bk antibodies  (Alomone Labs)


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  • 94

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    Alomone Labs polyclonal bk antibodies
    Polyclonal Bk Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    alomone apc  (Alomone Labs)


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    Alomone Labs alomone apc
    Alomone Apc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rb anti k v 3 3 kcn3 pab  (Alomone Labs)


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    Alomone Labs rb anti k v 3 3 kcn3 pab
    Primary and Secondary Antibody Sources and Working Dilutions.
    Rb Anti K V 3 3 Kcn3 Pab, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Immunohistochemical Characterization of the Chemosensory Pulmonary Neuroepithelial Bodies in the Naked Mole-Rat Reveals a Unique Adaptive Phenotype"

    Article Title: Immunohistochemical Characterization of the Chemosensory Pulmonary Neuroepithelial Bodies in the Naked Mole-Rat Reveals a Unique Adaptive Phenotype

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0112623

    Primary and Secondary Antibody Sources and Working Dilutions.
    Figure Legend Snippet: Primary and Secondary Antibody Sources and Working Dilutions.

    Techniques Used:

    rb anti k v 4 3 kcn4 pab  (Alomone Labs)


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    Alomone Labs rb anti k v 4 3 kcn4 pab
    Primary and Secondary Antibody Sources and Working Dilutions.
    Rb Anti K V 4 3 Kcn4 Pab, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Immunohistochemical Characterization of the Chemosensory Pulmonary Neuroepithelial Bodies in the Naked Mole-Rat Reveals a Unique Adaptive Phenotype"

    Article Title: Immunohistochemical Characterization of the Chemosensory Pulmonary Neuroepithelial Bodies in the Naked Mole-Rat Reveals a Unique Adaptive Phenotype

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0112623

    Primary and Secondary Antibody Sources and Working Dilutions.
    Figure Legend Snippet: Primary and Secondary Antibody Sources and Working Dilutions.

    Techniques Used:

    calcium modulated potassium channel bk  (Alomone Labs)


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    Alomone Labs calcium modulated potassium channel bk
    Calcium Modulated Potassium Channel Bk, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti α 1184 1200  (Alomone Labs)


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    Alomone Labs anti α 1184 1200
    Anti α 1184 1200, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal  (Alomone Labs)


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    Alomone Labs rabbit polyclonal
    (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a <t>polyclonal</t> antibody against prestin (green, E and F). Scale bar equals 10 µm.
    Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "BK Channels Mediate Cholinergic Inhibition of High Frequency Cochlear Hair Cells"

    Article Title: BK Channels Mediate Cholinergic Inhibition of High Frequency Cochlear Hair Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0013836

    (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.
    Figure Legend Snippet: (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.

    Techniques Used:

    (A) Whole-cell currents recorded from a basal outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence of either 100 µM linopirdine (red trace) or both 100 µM linopirdine and 100 nM IBTX (blue trace). (For clarity only 2 potentials, −131 mV and 49 mV are shown.) (B) At depolarizing membrane potentials, both linopirdine-sensitive KCNQ4 and IBTX-sensitive currents contribute to the voltage gated currents in basal outer hair cells. (C) Bar plot comparing the percent block of I Max at 49 mV in apical and basal OHCs in the presence of 100 µM linopirdine (black bars) or both 100 µM linopirdine and 100 nM IBTX (grey bars). (D) Confocal micrograph (1 optical section) of a basal cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) as well as immunoreactivity in the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.
    Figure Legend Snippet: (A) Whole-cell currents recorded from a basal outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence of either 100 µM linopirdine (red trace) or both 100 µM linopirdine and 100 nM IBTX (blue trace). (For clarity only 2 potentials, −131 mV and 49 mV are shown.) (B) At depolarizing membrane potentials, both linopirdine-sensitive KCNQ4 and IBTX-sensitive currents contribute to the voltage gated currents in basal outer hair cells. (C) Bar plot comparing the percent block of I Max at 49 mV in apical and basal OHCs in the presence of 100 µM linopirdine (black bars) or both 100 µM linopirdine and 100 nM IBTX (grey bars). (D) Confocal micrograph (1 optical section) of a basal cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) as well as immunoreactivity in the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.

    Techniques Used: Blocking Assay

    3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a monoclonal antibody against the BK channel (red) and a polyclonal antibody against synapsin (green) to label the efferent presynaptic terminals show that BK channel immunoreactivity in the three rows of outer hair cells (when present) is associated with efferent terminals. (D) Moreover, the size of BK channel immunopuncta increases from apical to middle and basal turns, paralleling an increase in size and likely number of efferent terminals. Data are presented as box plots representing the median (box interior), the 25th and 75th percentile (box boundaries), the 10th and 90th percentile (whiskers), and the 5th and 95th percentile (dots). (E) 3D renderings of confocal z-stacks of a middle cochlear turn immunostained with a polyclonal antibody against the BK channel (red) and a monoclonal antibody against the NaK-ATPase α3 (green) to label the efferent presynaptic terminal membrane shows adjacent but not co localized expression of the BK channel with the presynaptic efferent terminal membrane. The location of the three rows of outer hair cells are indicated (solid arrowheads). Grid dimensions equal 10 µm.
    Figure Legend Snippet: 3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a monoclonal antibody against the BK channel (red) and a polyclonal antibody against synapsin (green) to label the efferent presynaptic terminals show that BK channel immunoreactivity in the three rows of outer hair cells (when present) is associated with efferent terminals. (D) Moreover, the size of BK channel immunopuncta increases from apical to middle and basal turns, paralleling an increase in size and likely number of efferent terminals. Data are presented as box plots representing the median (box interior), the 25th and 75th percentile (box boundaries), the 10th and 90th percentile (whiskers), and the 5th and 95th percentile (dots). (E) 3D renderings of confocal z-stacks of a middle cochlear turn immunostained with a polyclonal antibody against the BK channel (red) and a monoclonal antibody against the NaK-ATPase α3 (green) to label the efferent presynaptic terminal membrane shows adjacent but not co localized expression of the BK channel with the presynaptic efferent terminal membrane. The location of the three rows of outer hair cells are indicated (solid arrowheads). Grid dimensions equal 10 µm.

    Techniques Used: Expressing

    3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green) show expression of the SK2 channel in the three rows of outer hair cells in all regions. Confocal z-stacks (23 optical sections) of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green, D) and monoclonal antibody against the BK channel (red, E) show colocalized expression (F) in the three rows of outer hair cells, further suggesting the localization of BK channels to the postsynaptic outer hair cell membrane. (Examination of single optical sections also shows colocalization of the SK2 and BK channel.) The size of the SK2 channel immunopuncta increases from apical to middle turns and then decreases in basal turns. Data are presented as box plots representing the median (box interior), the 25 th and 75 th percentile (box boundaries), the 10 th and 90 th percentile (whiskers), and the 5 th and 95 th percentile (dots). (G) 3D renderings of confocal z-stacks of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green), a monoclonal antibody against the BK channel (red), and a goat polyclonal antibody against synapsin (blue) confirm the colocalized expression of SK2 and BK channels in association with efferent terminals (H) in the three rows of outer hair cells. In basal turns, BK and SK2 channel immunoreactivity are colocalized. Grid dimensions equal 10 µm for top and bottom panels. Scale bar equals 10 µm for middle panels.
    Figure Legend Snippet: 3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green) show expression of the SK2 channel in the three rows of outer hair cells in all regions. Confocal z-stacks (23 optical sections) of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green, D) and monoclonal antibody against the BK channel (red, E) show colocalized expression (F) in the three rows of outer hair cells, further suggesting the localization of BK channels to the postsynaptic outer hair cell membrane. (Examination of single optical sections also shows colocalization of the SK2 and BK channel.) The size of the SK2 channel immunopuncta increases from apical to middle turns and then decreases in basal turns. Data are presented as box plots representing the median (box interior), the 25 th and 75 th percentile (box boundaries), the 10 th and 90 th percentile (whiskers), and the 5 th and 95 th percentile (dots). (G) 3D renderings of confocal z-stacks of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green), a monoclonal antibody against the BK channel (red), and a goat polyclonal antibody against synapsin (blue) confirm the colocalized expression of SK2 and BK channels in association with efferent terminals (H) in the three rows of outer hair cells. In basal turns, BK and SK2 channel immunoreactivity are colocalized. Grid dimensions equal 10 µm for top and bottom panels. Scale bar equals 10 µm for middle panels.

    Techniques Used: Expressing

    anti sk channel  (Alomone Labs)


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    Alomone Labs anti sk channel
    Anti Sk Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs bk ca
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    (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a <t>polyclonal</t> antibody against prestin (green, E and F). Scale bar equals 10 µm.
    Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti sk channel
    (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a <t>polyclonal</t> antibody against prestin (green, E and F). Scale bar equals 10 µm.
    Anti Sk Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Primary and Secondary Antibody Sources and Working Dilutions.

    Journal: PLoS ONE

    Article Title: Immunohistochemical Characterization of the Chemosensory Pulmonary Neuroepithelial Bodies in the Naked Mole-Rat Reveals a Unique Adaptive Phenotype

    doi: 10.1371/journal.pone.0112623

    Figure Lengend Snippet: Primary and Secondary Antibody Sources and Working Dilutions.

    Article Snippet: Rb anti-K v 3.3 (KCN3) pAb , 1∶800 , Alomone Lab.

    Techniques:

    Primary and Secondary Antibody Sources and Working Dilutions.

    Journal: PLoS ONE

    Article Title: Immunohistochemical Characterization of the Chemosensory Pulmonary Neuroepithelial Bodies in the Naked Mole-Rat Reveals a Unique Adaptive Phenotype

    doi: 10.1371/journal.pone.0112623

    Figure Lengend Snippet: Primary and Secondary Antibody Sources and Working Dilutions.

    Article Snippet: Rb anti-K v 4.3 (KCN4) pAb , 1∶500 , Alomone Lab.

    Techniques:

    (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.

    Journal: PLoS ONE

    Article Title: BK Channels Mediate Cholinergic Inhibition of High Frequency Cochlear Hair Cells

    doi: 10.1371/journal.pone.0013836

    Figure Lengend Snippet: (A) Whole-cell currents recorded from an apical outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence (grey line) of bath application of 100 nM IBTX, a specific blocker of BK channels, show little IBTX-sensitive currents. (For clarity only some of the traces are shown.) (B) No differences in voltage-gated currents were observed in response to IBTX application across all test potentials examined in apical outer hair cells. (C) Bar plot comparing the fractional contribution of IBTX- and linopirdine-sensitive currents to apical outer hair cells at 49 mV show that apical outer hair cells express predominantly linopirdine-sensitive KCNQ4 currents and little or no BK currents. Confocal micrograph (1 optical section) of an apical cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) but no immunoreactivity in the region associated with the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.

    Article Snippet: Immunofluorescent staining was performed as described previously using the following antibodies: mouse monoclonal anti-BK (L6/23; 1∶500; provided by Dr. James Trimmer, UC Davis, CA), rabbit polyclonal anti BK (APC021; 1∶500; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-SK2 (1∶500; provided by Dr John Adelman, Vollum Institute, University of Oregon Health Sciences), goat polyclonal anti-prestin (1∶300; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-synapsin (AB1543P; 1∶600; Chemicon/Millipore, Temecula, CA); and mouse monoclonal anti-Na-KATPase α3 subunit (MA3-915; 1∶600; Affinity Bioreagents, Rockford, IL).

    Techniques:

    (A) Whole-cell currents recorded from a basal outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence of either 100 µM linopirdine (red trace) or both 100 µM linopirdine and 100 nM IBTX (blue trace). (For clarity only 2 potentials, −131 mV and 49 mV are shown.) (B) At depolarizing membrane potentials, both linopirdine-sensitive KCNQ4 and IBTX-sensitive currents contribute to the voltage gated currents in basal outer hair cells. (C) Bar plot comparing the percent block of I Max at 49 mV in apical and basal OHCs in the presence of 100 µM linopirdine (black bars) or both 100 µM linopirdine and 100 nM IBTX (grey bars). (D) Confocal micrograph (1 optical section) of a basal cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) as well as immunoreactivity in the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.

    Journal: PLoS ONE

    Article Title: BK Channels Mediate Cholinergic Inhibition of High Frequency Cochlear Hair Cells

    doi: 10.1371/journal.pone.0013836

    Figure Lengend Snippet: (A) Whole-cell currents recorded from a basal outer hair cell in response to voltage steps (100 ms in duration) from −131 mV to 49 mV in 10 mV increments (with an interpulse holding potential of −81 mV) before (black line) and in the presence of either 100 µM linopirdine (red trace) or both 100 µM linopirdine and 100 nM IBTX (blue trace). (For clarity only 2 potentials, −131 mV and 49 mV are shown.) (B) At depolarizing membrane potentials, both linopirdine-sensitive KCNQ4 and IBTX-sensitive currents contribute to the voltage gated currents in basal outer hair cells. (C) Bar plot comparing the percent block of I Max at 49 mV in apical and basal OHCs in the presence of 100 µM linopirdine (black bars) or both 100 µM linopirdine and 100 nM IBTX (grey bars). (D) Confocal micrograph (1 optical section) of a basal cochlear turn immunostained with a monoclonal antibody against the BK channel (red, D and F) shows immunoreactivity in the region corresponding to the neck of the inner hair cells (indicated with an open arrowhead) as well as immunoreactivity in the outer hair cells (indicated by the solid arrowheads), immunostained with a polyclonal antibody against prestin (green, E and F). Scale bar equals 10 µm.

    Article Snippet: Immunofluorescent staining was performed as described previously using the following antibodies: mouse monoclonal anti-BK (L6/23; 1∶500; provided by Dr. James Trimmer, UC Davis, CA), rabbit polyclonal anti BK (APC021; 1∶500; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-SK2 (1∶500; provided by Dr John Adelman, Vollum Institute, University of Oregon Health Sciences), goat polyclonal anti-prestin (1∶300; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-synapsin (AB1543P; 1∶600; Chemicon/Millipore, Temecula, CA); and mouse monoclonal anti-Na-KATPase α3 subunit (MA3-915; 1∶600; Affinity Bioreagents, Rockford, IL).

    Techniques: Blocking Assay

    3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a monoclonal antibody against the BK channel (red) and a polyclonal antibody against synapsin (green) to label the efferent presynaptic terminals show that BK channel immunoreactivity in the three rows of outer hair cells (when present) is associated with efferent terminals. (D) Moreover, the size of BK channel immunopuncta increases from apical to middle and basal turns, paralleling an increase in size and likely number of efferent terminals. Data are presented as box plots representing the median (box interior), the 25th and 75th percentile (box boundaries), the 10th and 90th percentile (whiskers), and the 5th and 95th percentile (dots). (E) 3D renderings of confocal z-stacks of a middle cochlear turn immunostained with a polyclonal antibody against the BK channel (red) and a monoclonal antibody against the NaK-ATPase α3 (green) to label the efferent presynaptic terminal membrane shows adjacent but not co localized expression of the BK channel with the presynaptic efferent terminal membrane. The location of the three rows of outer hair cells are indicated (solid arrowheads). Grid dimensions equal 10 µm.

    Journal: PLoS ONE

    Article Title: BK Channels Mediate Cholinergic Inhibition of High Frequency Cochlear Hair Cells

    doi: 10.1371/journal.pone.0013836

    Figure Lengend Snippet: 3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a monoclonal antibody against the BK channel (red) and a polyclonal antibody against synapsin (green) to label the efferent presynaptic terminals show that BK channel immunoreactivity in the three rows of outer hair cells (when present) is associated with efferent terminals. (D) Moreover, the size of BK channel immunopuncta increases from apical to middle and basal turns, paralleling an increase in size and likely number of efferent terminals. Data are presented as box plots representing the median (box interior), the 25th and 75th percentile (box boundaries), the 10th and 90th percentile (whiskers), and the 5th and 95th percentile (dots). (E) 3D renderings of confocal z-stacks of a middle cochlear turn immunostained with a polyclonal antibody against the BK channel (red) and a monoclonal antibody against the NaK-ATPase α3 (green) to label the efferent presynaptic terminal membrane shows adjacent but not co localized expression of the BK channel with the presynaptic efferent terminal membrane. The location of the three rows of outer hair cells are indicated (solid arrowheads). Grid dimensions equal 10 µm.

    Article Snippet: Immunofluorescent staining was performed as described previously using the following antibodies: mouse monoclonal anti-BK (L6/23; 1∶500; provided by Dr. James Trimmer, UC Davis, CA), rabbit polyclonal anti BK (APC021; 1∶500; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-SK2 (1∶500; provided by Dr John Adelman, Vollum Institute, University of Oregon Health Sciences), goat polyclonal anti-prestin (1∶300; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-synapsin (AB1543P; 1∶600; Chemicon/Millipore, Temecula, CA); and mouse monoclonal anti-Na-KATPase α3 subunit (MA3-915; 1∶600; Affinity Bioreagents, Rockford, IL).

    Techniques: Expressing

    3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green) show expression of the SK2 channel in the three rows of outer hair cells in all regions. Confocal z-stacks (23 optical sections) of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green, D) and monoclonal antibody against the BK channel (red, E) show colocalized expression (F) in the three rows of outer hair cells, further suggesting the localization of BK channels to the postsynaptic outer hair cell membrane. (Examination of single optical sections also shows colocalization of the SK2 and BK channel.) The size of the SK2 channel immunopuncta increases from apical to middle turns and then decreases in basal turns. Data are presented as box plots representing the median (box interior), the 25 th and 75 th percentile (box boundaries), the 10 th and 90 th percentile (whiskers), and the 5 th and 95 th percentile (dots). (G) 3D renderings of confocal z-stacks of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green), a monoclonal antibody against the BK channel (red), and a goat polyclonal antibody against synapsin (blue) confirm the colocalized expression of SK2 and BK channels in association with efferent terminals (H) in the three rows of outer hair cells. In basal turns, BK and SK2 channel immunoreactivity are colocalized. Grid dimensions equal 10 µm for top and bottom panels. Scale bar equals 10 µm for middle panels.

    Journal: PLoS ONE

    Article Title: BK Channels Mediate Cholinergic Inhibition of High Frequency Cochlear Hair Cells

    doi: 10.1371/journal.pone.0013836

    Figure Lengend Snippet: 3D renderings of confocal z-stacks of apical (A), middle (B), and basal (C) cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green) show expression of the SK2 channel in the three rows of outer hair cells in all regions. Confocal z-stacks (23 optical sections) of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green, D) and monoclonal antibody against the BK channel (red, E) show colocalized expression (F) in the three rows of outer hair cells, further suggesting the localization of BK channels to the postsynaptic outer hair cell membrane. (Examination of single optical sections also shows colocalization of the SK2 and BK channel.) The size of the SK2 channel immunopuncta increases from apical to middle turns and then decreases in basal turns. Data are presented as box plots representing the median (box interior), the 25 th and 75 th percentile (box boundaries), the 10 th and 90 th percentile (whiskers), and the 5 th and 95 th percentile (dots). (G) 3D renderings of confocal z-stacks of basal cochlear turns immunostained with a polyclonal antibody against the SK2 channel (green), a monoclonal antibody against the BK channel (red), and a goat polyclonal antibody against synapsin (blue) confirm the colocalized expression of SK2 and BK channels in association with efferent terminals (H) in the three rows of outer hair cells. In basal turns, BK and SK2 channel immunoreactivity are colocalized. Grid dimensions equal 10 µm for top and bottom panels. Scale bar equals 10 µm for middle panels.

    Article Snippet: Immunofluorescent staining was performed as described previously using the following antibodies: mouse monoclonal anti-BK (L6/23; 1∶500; provided by Dr. James Trimmer, UC Davis, CA), rabbit polyclonal anti BK (APC021; 1∶500; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-SK2 (1∶500; provided by Dr John Adelman, Vollum Institute, University of Oregon Health Sciences), goat polyclonal anti-prestin (1∶300; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-synapsin (AB1543P; 1∶600; Chemicon/Millipore, Temecula, CA); and mouse monoclonal anti-Na-KATPase α3 subunit (MA3-915; 1∶600; Affinity Bioreagents, Rockford, IL).

    Techniques: Expressing