Paxilline, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Contributions of CaV1.3 Channels to Ca2+ Current and Ca2+-Activated BK Current in the Suprachiasmatic Nucleus"
Article Title: Contributions of CaV1.3 Channels to Ca2+ Current and Ca2+-Activated BK Current in the Suprachiasmatic Nucleus
Journal: Frontiers in Physiology
Figure Legend Snippet: Effects of Ca 2+ chelators and Ca 2+ channel inhibitors on BK currents recorded from SCN neurons during the day and night. Paxilline-sensitive macroscopic BK currents were recorded from C57BL6 WT (A–E) and Ca V 1.3 WT (F) SCNs. All intracellular solutions in this study were made with 0.9 mM EGTA, except where 5 mM BAPTA was substituted (A–E) . Currents were elicited from a holding potential of −90 mV by 150-ms voltage steps from −110 to +90 mV in +20-mV increments. (A,B) Representative BK currents from −90 to +90 mV are shown from day (A) and night (B) SCN neurons. (C,D) Current-voltage plot comparing BK current density recorded in either control EGTA or BAPTA during the day (C) and night (D) . (E) Summary of BK current density at +90 mV recorded with control EGTA or BAPTA with Ca 2+ channel inhibitors 10 μM nimodipine (Nim) during the day, or 10 μM dantrolene (Dan) at night. In EGTA, Nim decreased BK currents compared to controls ( P = 0.0008), with no significant difference in BAPTA. At night, BK currents were decreased with BAPTA compared to control EGTA conditions ( P
Figure Legend Snippet: BK currents from Ca V 1.3 WT and Ca V 1.3 KO SCN during the day and night. Paxilline-sensitive macroscopic BK currents were recorded as in Figure 1 . (A) Summary of BK current densities at +90 mV recorded from Ca V 1.3 WT and Ca V 1.3 KO neurons during the day and night. BK currents were increased at night for both Ca V 1.3 WT ( P = 0.004) and Ca V 1.3 KO ( P = 0.007) compared to the day. * P