Journal: International Journal of Molecular Sciences
Article Title: In Vitro Evaluation of the Influence of Biosynthesized Calcium Oxide Nanoparticles on the Antibacterial Activity, pH, Microleakage and Cytotoxicity of Conventional Intracanal Medicaments
doi: 10.3390/ijms252211991
Figure Lengend Snippet: BJ cell line human fibroblasts ( a ). cultured in the flask (intra-experiment), ( b ). in a 96-well plate for cytotoxicity evaluation with the MTT Assay (intra-experiment). ( c , d ) Pre-experiment BJ cell line fibroblasts as observed under a microscope. Cells were obtained from ATCC (CRL-2522). Image ( c , d ) courtesy: ATCC, https://www.atcc.org/products/crl-2522#product-references (accessed on 27 October 2024).
Article Snippet: BJ human fibroblast cells (ATCC # CRL-2522) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum, 100 IU/mL of penicillin, 100 µg/mL of streptomycin and 2 mM L-glutamine in 75 cm flasks, incubated in 5% CO at 37 °C.
Techniques: Cell Culture, MTT Assay, Microscopy