bisulfite treated control dna samples  (Qiagen)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    EpiTect Control DNA
    Description:
    For control experiments during methylation analysis Kit contents Qiagen EpiTect Control DNA Unmethylated Human Control DNA for 1000 Control PCRs Convenient Ready to use Quality controlled DNA Ideal for all Methylation Analyses Evaluating Primer Specificity for MSP Quantification standard for HRM and MethyLight PCR Evaluating Primer and Probe Specificity for MethyLight PCR Checking Efficiency of Bisulfite Conversion Reactions Benefits Convenient ready to use quality controlled DNA Bisulfite converted DNA for control experiments Suitable for all methylation analyses
    Catalog Number:
    59568
    Price:
    206
    Category:
    EpiTect Control DNA and Control DNA Set
    Buy from Supplier


    Structured Review

    Qiagen bisulfite treated control dna samples
    EpiTect Control DNA
    For control experiments during methylation analysis Kit contents Qiagen EpiTect Control DNA Unmethylated Human Control DNA for 1000 Control PCRs Convenient Ready to use Quality controlled DNA Ideal for all Methylation Analyses Evaluating Primer Specificity for MSP Quantification standard for HRM and MethyLight PCR Evaluating Primer and Probe Specificity for MethyLight PCR Checking Efficiency of Bisulfite Conversion Reactions Benefits Convenient ready to use quality controlled DNA Bisulfite converted DNA for control experiments Suitable for all methylation analyses
    https://www.bioz.com/result/bisulfite treated control dna samples/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bisulfite treated control dna samples - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    qpcr

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: The prognostic value of BRCA1 promoter methylation in early stage triple negative breast cancer
    Article Snippet: .. Specificity of the reactions was confirmed using the EpiTect® Control DNA set (Qiagen) with the same primers and PCR conditions. .. The presence of a methylated band was recorded as “positive” for BRCA1 PM ( ).

    Article Title: ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells
    Article Snippet: .. Methylated human control DNA from EpiTect PCR Control DNA Set (Qiagen) was used as positive control. .. Western blotting Whole-cell lysates were extracted using RIPA buffer and used for immunoblotting according to standard procedures using primary antibody against ESRP1 (Sigma). β-actin (Santa Cruz Biotechnology, Dallas, TX, USA; sc-47778) was used as a loading control.

    Article Title: Methylation and Loss of Secreted Frizzled-Related Protein 3 Enhances Melanoma Cell Migration and Invasion
    Article Snippet: .. Universally unmethylated and methylated DNA were used as positive and negative controls respectively for methylation specific PCR (EpiTect Control DNAs, Qiagen). .. Methylation specific PCR reactions were carried out as described above (all primer sequences are listed in ).

    Concentration Assay:

    Article Title: Detection of Methylated Septin 9 in Tissue and Plasma of Colorectal Patients with Neoplasia and the Relationship to the Amount of Circulating Cell-Free DNA
    Article Snippet: .. Standard curve was used for quantitative measurements using EpiTect bisulfite converted, fully methylated control DNA (Qiagen) in concentration steps from 30; 15; 5; 2 to 0.8 ng/PCR in each RT-PCR run. (DOCX) Click here for additional data file. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Detection of Methylated Septin 9 in Tissue and Plasma of Colorectal Patients with Neoplasia and the Relationship to the Amount of Circulating Cell-Free DNA
    Article Snippet: .. Standard curve was used for quantitative measurements using EpiTect bisulfite converted, fully methylated control DNA (Qiagen) in concentration steps from 30; 15; 5; 2 to 0.8 ng/PCR in each RT-PCR run. (DOCX) Click here for additional data file. ..

    Positive Control:

    Article Title: ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells
    Article Snippet: .. Methylated human control DNA from EpiTect PCR Control DNA Set (Qiagen) was used as positive control. .. Western blotting Whole-cell lysates were extracted using RIPA buffer and used for immunoblotting according to standard procedures using primary antibody against ESRP1 (Sigma). β-actin (Santa Cruz Biotechnology, Dallas, TX, USA; sc-47778) was used as a loading control.

    Methylation:

    Article Title: Detection of Methylated Septin 9 in Tissue and Plasma of Colorectal Patients with Neoplasia and the Relationship to the Amount of Circulating Cell-Free DNA
    Article Snippet: .. Standard curve was used for quantitative measurements using EpiTect bisulfite converted, fully methylated control DNA (Qiagen) in concentration steps from 30; 15; 5; 2 to 0.8 ng/PCR in each RT-PCR run. (DOCX) Click here for additional data file. ..

    Article Title: Epigenetic modifications at DMRs of placental genes are subjected to variations in normal gestation, pathological conditions and folate supplementation
    Article Snippet: .. For the 0% methylation standard we have used commercially available control DNA from Qiagen (EpiTect® Control DNA), while the enzymatic treatment of genomic DNA was used to synthesize fully methylated DNA (100% methylation standard) using M.SssI enzyme (CpG Methyltransferase, New England Biolabs, Beverly, MA, USA). .. These methylation standards were also subjected to bisulfite conversion.

    Article Title: Prognostic relevance of Wnt-inhibitory factor-1 (WIF1) and Dickkopf-3 (DKK3) promoter methylation in human breast cancer
    Article Snippet: .. Specificity of MSP primers in detecting the promoter methylation status were demonstrated by use of universal unmethylated and universal poly-methylated DNA as template (Epi Tect Control DNA Set; Qiagen, Hilden, Germany). .. Sensitivity of the utilized primers and cycling conditions was defined by use of a dilution series in MSP assays, constituted of methylated DNA diluted with unmethylated DNA (50%, 10%, 1%, 0.1%, 0.01% and 0% methylation).

    Article Title: Genome-Wide Screening of Genes Regulated by DNA Methylation in Colon Cancer Development
    Article Snippet: .. For calibration of the analysis, 0%, 2%, 25%, 50%, 75% and 100% artificially methylated DNA samples were prepared by mixing methylated (Universal Methylated Human DNA Standard, Zymo Research) and unmethylated (Unmethlyated EpiTect Control DNA, Qiagen) samples in the appropriate ratios. .. From each template 300 ng DNA was bisulfite converted by methylSEQr kit (Applied Biosystems) according to the manufacturer’s instructions.

    Article Title: JAM3 methylation status as a biomarker for diagnosis of preneoplastic and neoplastic lesions of the cervix
    Article Snippet: .. EpiTect Control DNA and Control DNA Sets (QIAGEN, Germany; containing both bisulfite-converted methylated and unmethylated DNA, and unconverted unmethylated DNA) were used as MSP and QMSP control DNA. .. In addition, PCR of the bisulfite converted housekeeping gene β-actin was performed as a reference.

    Article Title: ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells
    Article Snippet: .. Methylated human control DNA from EpiTect PCR Control DNA Set (Qiagen) was used as positive control. .. Western blotting Whole-cell lysates were extracted using RIPA buffer and used for immunoblotting according to standard procedures using primary antibody against ESRP1 (Sigma). β-actin (Santa Cruz Biotechnology, Dallas, TX, USA; sc-47778) was used as a loading control.

    Article Title: Methylation and Loss of Secreted Frizzled-Related Protein 3 Enhances Melanoma Cell Migration and Invasion
    Article Snippet: .. Universally unmethylated and methylated DNA were used as positive and negative controls respectively for methylation specific PCR (EpiTect Control DNAs, Qiagen). .. Methylation specific PCR reactions were carried out as described above (all primer sequences are listed in ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen bisulfite treated control dna samples
    Bisulfite Treated Control Dna Samples, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisulfite treated control dna samples/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bisulfite treated control dna samples - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results