biotinylated  (Vector Laboratories)


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    Name:
    Biotinylated Concanavalin A Con A
    Description:
    Concanavalin A Con A recognizes α linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide
    Catalog Number:
    b-1005
    Price:
    None
    Category:
    Proteins
    Size:
    5 mg
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated
    Biotinylated Concanavalin A Con A
    Concanavalin A Con A recognizes α linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide
    https://www.bioz.com/result/biotinylated/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated - by Bioz Stars, 2021-03
    96/100 stars

    Images

    1) Product Images from "Interleukin 6 Influences Germinal Center Development and Antibody Production via a Contribution of C3 Complement Component "

    Article Title: Interleukin 6 Influences Germinal Center Development and Antibody Production via a Contribution of C3 Complement Component

    Journal: The Journal of Experimental Medicine

    doi:

    Expression of B7.2 is similar while B7.1 is upregulated on germinal center B cells of IL-6–deficient as compared with wild-type mice. Germinal center cells were isolated as described in the Materials and Methods section. The B cells were gated on using B220 conjugated to PE and then the level of B7.1 ( A ) and B7.2 ( B ) determined using biotinylated reagents plus avidin-FITC. The results are presented as levels on the wild-type derived ( solid histograms ) versus germinal center B cells obtained from the deficient mice ( open histograms ). Isotype controls were similar to the level seen with B7.1 on wild-type derived cells.
    Figure Legend Snippet: Expression of B7.2 is similar while B7.1 is upregulated on germinal center B cells of IL-6–deficient as compared with wild-type mice. Germinal center cells were isolated as described in the Materials and Methods section. The B cells were gated on using B220 conjugated to PE and then the level of B7.1 ( A ) and B7.2 ( B ) determined using biotinylated reagents plus avidin-FITC. The results are presented as levels on the wild-type derived ( solid histograms ) versus germinal center B cells obtained from the deficient mice ( open histograms ). Isotype controls were similar to the level seen with B7.1 on wild-type derived cells.

    Techniques Used: Expressing, Mouse Assay, Isolation, Avidin-Biotin Assay, Derivative Assay

    Related Articles

    Incubation:

    Article Title: AdcAII of Streptococcus pneumoniae Affects Pneumococcal Invasiveness
    Article Snippet: AdcAII was biotin labeled using the Biotin Labeling Kit (Roche, Indianapolis, IN) per manufacturer’s instructions. .. After incubation with biotinylated AdcAII, the blot was washed with PBST and incubated with horseradish peroxidase strepavidin (1:5000) (Vector Laboratories, Burlingame, CA) prior to another wash and development using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL). .. For a laminin-binding control, recombinant 37/67kDa laminin receptor protein was used as a probe and developed using anti-LR mAb (1:500) (Abcam, Cambridge, MA) and HRP-conjugated goat anti-mouse secondary (1:10000) (Bio-Rad).

    Article Title: An Endoplasmic Reticulum Protein, p180, Is Highly Expressed in Human Cytomegalovirus-Permissive Cells and Interacts with the Tegument Protein Encoded by UL48
    Article Snippet: After extensive washing with buffer A (150 mM NaCl, 1 mM EDTA, 1% gelatin, 10 mM Tris-HCl, pH 7.0), radioactive bands were detected with an image analyzer (BAS 1000; Fuji Photofilm, Kanagawa, Japan). .. Alternatively, the membrane was incubated with biotinylated virions in place of radiolabeled virions and then with streptavidin conjugated with horseradish peroxidase (Vector Laboratory, Burlingame, Calif.). .. The positive bands were detected by an ECL system (Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, United Kingdom).

    Article Title: A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors
    Article Snippet: Samples were applied to the rehydrated slide in TSM buffer containing 1% BSA and 0.05% Tween 20 in a final volume of 100 μl and the slides were incubated in a humidified chamber at room temperature for 1 h. After incubation, sample was washed away by gently dipping the slides in buffer contained in Coplin jars. .. Biotinylated ConA (Vector Labs) lectin binding to glycans on the array was detected by a secondary incubation with streptavidin-cyanine 5 at 5 μg/ml (SA-Cy5). .. The slides were scanned with a Genepix fluorescence microarray scanner equipped with four lasers covering an excitation range from 488 to 637 nm.

    Amplification:

    Article Title: Masked mRNA is stored with aggregated nuclear speckles and its asymmetric redistribution requires a homolog of mago nashi
    Article Snippet: Detection of biotinylated FISH probes was achieved through the use of a goat-anti-biotin antibody, followed by a rabbit-anti-goat antibody conjugated with AlexaFluor 594. .. Alternatively, biotinylated probes requiring signal amplification were detected using either TexasRed or Fluorescein labeled Avidin (Vector Laboratories A-2016 and A20-11) followed by biotinylated anti-Avidin D (Vector Laboratories BA-0300) labeling and a second round of TexasRed or Fluorescein labeled Avidin labeling following the manufacturers instructions. ..

    Labeling:

    Article Title: Masked mRNA is stored with aggregated nuclear speckles and its asymmetric redistribution requires a homolog of mago nashi
    Article Snippet: Detection of biotinylated FISH probes was achieved through the use of a goat-anti-biotin antibody, followed by a rabbit-anti-goat antibody conjugated with AlexaFluor 594. .. Alternatively, biotinylated probes requiring signal amplification were detected using either TexasRed or Fluorescein labeled Avidin (Vector Laboratories A-2016 and A20-11) followed by biotinylated anti-Avidin D (Vector Laboratories BA-0300) labeling and a second round of TexasRed or Fluorescein labeled Avidin labeling following the manufacturers instructions. ..

    Avidin-Biotin Assay:

    Article Title: Masked mRNA is stored with aggregated nuclear speckles and its asymmetric redistribution requires a homolog of mago nashi
    Article Snippet: Detection of biotinylated FISH probes was achieved through the use of a goat-anti-biotin antibody, followed by a rabbit-anti-goat antibody conjugated with AlexaFluor 594. .. Alternatively, biotinylated probes requiring signal amplification were detected using either TexasRed or Fluorescein labeled Avidin (Vector Laboratories A-2016 and A20-11) followed by biotinylated anti-Avidin D (Vector Laboratories BA-0300) labeling and a second round of TexasRed or Fluorescein labeled Avidin labeling following the manufacturers instructions. ..

    Activity Assay:

    Article Title: EndoE from Enterococcus faecalis Hydrolyzes the Glycans of the Biofilm Inhibiting Protein Lactoferrin and Mediates Growth
    Article Snippet: Briefly, 2 µg of hLF was incubated with 2 µg recombinant EndoE, EndoE(E186Q) or EndoE(E662Q) in a total volume of 20 µl of PBS buffer for 16 h at 37°C. .. Activity was analyzed with 10% SDS PAGE (stained with Coomassie Brilliant Blue G-250) and lectin blot analysis was performed using 0.5 µg/ml biotinylated ConA lectin (Vector Laboratories, Burlingame, CA) , . .. UHPLC Analysis Glycans from 1 µg hLF were released with EndoE or N-glycosidase F (PNGaseF; ProZyme) in PBS at 37°C overnight.

    SDS Page:

    Article Title: EndoE from Enterococcus faecalis Hydrolyzes the Glycans of the Biofilm Inhibiting Protein Lactoferrin and Mediates Growth
    Article Snippet: Briefly, 2 µg of hLF was incubated with 2 µg recombinant EndoE, EndoE(E186Q) or EndoE(E662Q) in a total volume of 20 µl of PBS buffer for 16 h at 37°C. .. Activity was analyzed with 10% SDS PAGE (stained with Coomassie Brilliant Blue G-250) and lectin blot analysis was performed using 0.5 µg/ml biotinylated ConA lectin (Vector Laboratories, Burlingame, CA) , . .. UHPLC Analysis Glycans from 1 µg hLF were released with EndoE or N-glycosidase F (PNGaseF; ProZyme) in PBS at 37°C overnight.

    Staining:

    Article Title: EndoE from Enterococcus faecalis Hydrolyzes the Glycans of the Biofilm Inhibiting Protein Lactoferrin and Mediates Growth
    Article Snippet: Briefly, 2 µg of hLF was incubated with 2 µg recombinant EndoE, EndoE(E186Q) or EndoE(E662Q) in a total volume of 20 µl of PBS buffer for 16 h at 37°C. .. Activity was analyzed with 10% SDS PAGE (stained with Coomassie Brilliant Blue G-250) and lectin blot analysis was performed using 0.5 µg/ml biotinylated ConA lectin (Vector Laboratories, Burlingame, CA) , . .. UHPLC Analysis Glycans from 1 µg hLF were released with EndoE or N-glycosidase F (PNGaseF; ProZyme) in PBS at 37°C overnight.

    Binding Assay:

    Article Title: A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors
    Article Snippet: Samples were applied to the rehydrated slide in TSM buffer containing 1% BSA and 0.05% Tween 20 in a final volume of 100 μl and the slides were incubated in a humidified chamber at room temperature for 1 h. After incubation, sample was washed away by gently dipping the slides in buffer contained in Coplin jars. .. Biotinylated ConA (Vector Labs) lectin binding to glycans on the array was detected by a secondary incubation with streptavidin-cyanine 5 at 5 μg/ml (SA-Cy5). .. The slides were scanned with a Genepix fluorescence microarray scanner equipped with four lasers covering an excitation range from 488 to 637 nm.

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  • 98
    Vector Laboratories biotinylated isolectin b4
    FAK and its kinase activity are required for VEGF-induced proliferation and migration. ( A , B ) Representative images of <t>isolectin</t> B4 (green) and pH3 (red) staining of whole-mount retinal vasculatures of control, cKO and cKD mice at P5 ( A ) and quantification of mitotic ECs (pH3 positive, IB4 positive, marked by arrows) per EC area. pH3 positive, IB4 negative cells are mitotic non-ECs, marked by asterisks. ( B ). Scale bar = 100 μm. n = 5, mean ± SEM. *p
    Biotinylated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated isolectin b4/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated isolectin b4 - by Bioz Stars, 2021-03
    98/100 stars
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    99
    Vector Laboratories biotinylated antirat igg
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Biotinylated Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirat igg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated antirat igg - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    FAK and its kinase activity are required for VEGF-induced proliferation and migration. ( A , B ) Representative images of isolectin B4 (green) and pH3 (red) staining of whole-mount retinal vasculatures of control, cKO and cKD mice at P5 ( A ) and quantification of mitotic ECs (pH3 positive, IB4 positive, marked by arrows) per EC area. pH3 positive, IB4 negative cells are mitotic non-ECs, marked by asterisks. ( B ). Scale bar = 100 μm. n = 5, mean ± SEM. *p

    Journal: Scientific Reports

    Article Title: Nuclear FAK and its kinase activity regulate VEGFR2 transcription in angiogenesis of adult mice

    doi: 10.1038/s41598-018-20930-z

    Figure Lengend Snippet: FAK and its kinase activity are required for VEGF-induced proliferation and migration. ( A , B ) Representative images of isolectin B4 (green) and pH3 (red) staining of whole-mount retinal vasculatures of control, cKO and cKD mice at P5 ( A ) and quantification of mitotic ECs (pH3 positive, IB4 positive, marked by arrows) per EC area. pH3 positive, IB4 negative cells are mitotic non-ECs, marked by asterisks. ( B ). Scale bar = 100 μm. n = 5, mean ± SEM. *p

    Article Snippet: Retinas were dissected, permeabilized and blocked in PBS containing 1% BSA and 0.5% Triton at 4 °C overnight, washed in PBLec (1% Triton X-100, 1 mM CaCl2 , 1 mM MgCl2 , and 0.1 mM MnCl2 in PBS), and incubated overnight in PBLec containing biotinylated isolectin B4 (1:25, Vector Labs).

    Techniques: Activity Assay, Migration, Staining, Mouse Assay

    Differentiation of CD9-positive cells into endothelial cells. ( A ) Bright field images of primary cultured CD9-positive cells cultured for 120 h on laminin-coated surfaces in medium with 0.1% BSA (left) or 10% FBS (right) at 2.0 × 10 4 cells/cm 2 . ( B ) Immunocytochemistry of VE-cadherin (left, green ) and in situ hybridisation of Kdr (right, arrowhead ) after cultivation of CD9-positive cells for 120 h with 10% FBS. ( C ) Double-staining of isolectin B4 with CD9 (upper row), S100β (middle row), and SOX2 (lower row) in rat CD9-positive cells after primary culture for 120 h with 10% FBS. ( D ) Relative ratio of mRNA levels of the following genes after primary culture of CD9-positive cells with 10% FBS ( white bar ) or 0.1% BSA ( black bar ) as determined by qPCR (mean ± SEM, n = 3), followed by normalisation with an internal control ( Actb ): Left graph: Cd9 , S100β, Sox2, Prop1, Cadh1 , and Cxcr4 . Right graph: Id2, Sox18 , Nrp1 , Kdr , Pecam1 , and Edn .

    Journal: Scientific Reports

    Article Title: Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats

    doi: 10.1038/s41598-018-23923-0

    Figure Lengend Snippet: Differentiation of CD9-positive cells into endothelial cells. ( A ) Bright field images of primary cultured CD9-positive cells cultured for 120 h on laminin-coated surfaces in medium with 0.1% BSA (left) or 10% FBS (right) at 2.0 × 10 4 cells/cm 2 . ( B ) Immunocytochemistry of VE-cadherin (left, green ) and in situ hybridisation of Kdr (right, arrowhead ) after cultivation of CD9-positive cells for 120 h with 10% FBS. ( C ) Double-staining of isolectin B4 with CD9 (upper row), S100β (middle row), and SOX2 (lower row) in rat CD9-positive cells after primary culture for 120 h with 10% FBS. ( D ) Relative ratio of mRNA levels of the following genes after primary culture of CD9-positive cells with 10% FBS ( white bar ) or 0.1% BSA ( black bar ) as determined by qPCR (mean ± SEM, n = 3), followed by normalisation with an internal control ( Actb ): Left graph: Cd9 , S100β, Sox2, Prop1, Cadh1 , and Cxcr4 . Right graph: Id2, Sox18 , Nrp1 , Kdr , Pecam1 , and Edn .

    Article Snippet: Cultured cells fixed with 4% paraformaldehyde in 0.025 M PB for 20 min at room temperature (21–23 °C) were first immersed in phosphate-buffered saline (PBS) containing 2% normal goat or donkey serum for 20 min at 30 °C, then incubated overnight with biotinylated isolectin B4 (1:25; Vector Laboratories) or primary antibodies as listed in Supplementary Table at room temperature.

    Techniques: Cell Culture, Immunocytochemistry, In Situ, Hybridization, Double Staining, Real-time Polymerase Chain Reaction

    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1

    doi:

    Figure Lengend Snippet: TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Article Snippet: Serial sections were stained with antibodies to CD31, CD102, CD144, CD106, Flk-1, and CD62E at 1 µg/ml, detected with biotinylated antirat IgG, and developed with the NovaRED substrate kit (Vector Laboratories).

    Techniques: Staining, Fluorescence, Inverted Microscopy, Software, Generated

    Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

    doi: 10.1186/s12964-018-0249-7

    Figure Lengend Snippet: Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Article Snippet: Sections were blocked in 5% swine serum (Sigma) and 2% BSA (Sigma), incubated with biotinylated 3G9 antibody and then with Fluorescein Avidin D (Vector Labs).

    Techniques: Staining, Immunofluorescence, Expressing