biotinylated wisteria floribunda agglutinin wfa  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa
    Biotinylated Wisteria Floribunda Agglutinin Wfa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin

    Biotinylated Wisteria Floribunda Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Restoring vision in adult amblyopia by enhancing plasticity through deletion of the transcriptional repressor REST"

    Article Title: Restoring vision in adult amblyopia by enhancing plasticity through deletion of the transcriptional repressor REST

    Journal: iScience

    doi: 10.1016/j.isci.2024.109507


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Virus, Software

    biotinylated wisteria floribunda agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin
    a . Schematic of experimental timeline of viral vector injections and electrophysiology experiments in critical period mice. b . Whole-cell patch clamp recordings of layer 2/3 pyramidal neurons (left) or fast spiking neurons (right). c . Representative spontaneous excitatory postsynaptic currents (sEPSCs) from each of the four conditions. d . Average interevent interval (ms). e . Average amplitude (pA). f . Average area (ms/pA). d-f . Black symbols denote individual cell averages. tdT no MD: n = 17 cells, 9 mice. tdT MD: n = 16 cells, 7 mice. CCN1 no MD: n = 13 cells, 7 mice. CCN1 MD: n = 16 cells, 6 mice. g . Representative sEPSCs as in c from fast-spiking neurons. h . Average interevent interval (ms). i . Average amplitude (pA). j . Average area (ms/pA). h-j . Black symbols denote individual cell averages. tdT no MD: n = 16 cells, 10 mice. tdT MD: n = 14 cells, 9 mice. CCN1 no MD: n = 12 cells, 7 mice. CCN1 MD: n = 10 cells, 6 mice. Two-way ANOVA with post-hoc Tukey tests. k . Representative images of wisteria <t>floribunda</t> <t>agglutinin</t> (WFA) and parvalbumin (PV) staining in critical period VC. Scale bar, 20 µm. l . WFA integrated density around PV+ cells. 5 mice per group. Tdt no MD, n = 204 cells. tdT MD, n = 197 cells. CCN1 no MD, n = 228 cells. CCN1 MD, n = 193 cells. Kruskal Wallis test with post-hoc Dunn’s tests. m. Representative image of IBA1and CD68 staining in critical period VC. Scale bar, 10 µm . n. Volume of CD68+ puncta within IBA1 volume. Kruskal Wallis test with post-hoc Dunn’s tests. o . Representative image of IBA1 staining from critical period VC, top. Representative cell body and process tracing (skeleton), bottom. Scale bar, 10 µm. tdT no MD, 747 microglia from 4 mice. tdT MD, 876 microglia from 4 mice. CCN1 no MD, 496 microglia from 3 mice. CCN1 MD, 379 microglia from 3 mice. p . Proportion of microglial morphotypes-amoeboid, rod-like, ramified, and hyper-ramified. Black symbols, mouse averages. Two-way ANOVA with post-hoc Tukey tests. Only significant tests are shown. Bar charts show mean ± SEM. Box charts show median, upper and lower quartiles and whiskers show full range of data.
    Biotinylated Wisteria Floribunda Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated wisteria floribunda agglutinin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated wisteria floribunda agglutinin - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Astrocyte CCN1 stabilizes neural circuits in the adult brain"

    Article Title: Astrocyte CCN1 stabilizes neural circuits in the adult brain

    Journal: bioRxiv

    doi: 10.1101/2024.03.14.585077

    a . Schematic of experimental timeline of viral vector injections and electrophysiology experiments in critical period mice. b . Whole-cell patch clamp recordings of layer 2/3 pyramidal neurons (left) or fast spiking neurons (right). c . Representative spontaneous excitatory postsynaptic currents (sEPSCs) from each of the four conditions. d . Average interevent interval (ms). e . Average amplitude (pA). f . Average area (ms/pA). d-f . Black symbols denote individual cell averages. tdT no MD: n = 17 cells, 9 mice. tdT MD: n = 16 cells, 7 mice. CCN1 no MD: n = 13 cells, 7 mice. CCN1 MD: n = 16 cells, 6 mice. g . Representative sEPSCs as in c from fast-spiking neurons. h . Average interevent interval (ms). i . Average amplitude (pA). j . Average area (ms/pA). h-j . Black symbols denote individual cell averages. tdT no MD: n = 16 cells, 10 mice. tdT MD: n = 14 cells, 9 mice. CCN1 no MD: n = 12 cells, 7 mice. CCN1 MD: n = 10 cells, 6 mice. Two-way ANOVA with post-hoc Tukey tests. k . Representative images of wisteria floribunda agglutinin (WFA) and parvalbumin (PV) staining in critical period VC. Scale bar, 20 µm. l . WFA integrated density around PV+ cells. 5 mice per group. Tdt no MD, n = 204 cells. tdT MD, n = 197 cells. CCN1 no MD, n = 228 cells. CCN1 MD, n = 193 cells. Kruskal Wallis test with post-hoc Dunn’s tests. m. Representative image of IBA1and CD68 staining in critical period VC. Scale bar, 10 µm . n. Volume of CD68+ puncta within IBA1 volume. Kruskal Wallis test with post-hoc Dunn’s tests. o . Representative image of IBA1 staining from critical period VC, top. Representative cell body and process tracing (skeleton), bottom. Scale bar, 10 µm. tdT no MD, 747 microglia from 4 mice. tdT MD, 876 microglia from 4 mice. CCN1 no MD, 496 microglia from 3 mice. CCN1 MD, 379 microglia from 3 mice. p . Proportion of microglial morphotypes-amoeboid, rod-like, ramified, and hyper-ramified. Black symbols, mouse averages. Two-way ANOVA with post-hoc Tukey tests. Only significant tests are shown. Bar charts show mean ± SEM. Box charts show median, upper and lower quartiles and whiskers show full range of data.
    Figure Legend Snippet: a . Schematic of experimental timeline of viral vector injections and electrophysiology experiments in critical period mice. b . Whole-cell patch clamp recordings of layer 2/3 pyramidal neurons (left) or fast spiking neurons (right). c . Representative spontaneous excitatory postsynaptic currents (sEPSCs) from each of the four conditions. d . Average interevent interval (ms). e . Average amplitude (pA). f . Average area (ms/pA). d-f . Black symbols denote individual cell averages. tdT no MD: n = 17 cells, 9 mice. tdT MD: n = 16 cells, 7 mice. CCN1 no MD: n = 13 cells, 7 mice. CCN1 MD: n = 16 cells, 6 mice. g . Representative sEPSCs as in c from fast-spiking neurons. h . Average interevent interval (ms). i . Average amplitude (pA). j . Average area (ms/pA). h-j . Black symbols denote individual cell averages. tdT no MD: n = 16 cells, 10 mice. tdT MD: n = 14 cells, 9 mice. CCN1 no MD: n = 12 cells, 7 mice. CCN1 MD: n = 10 cells, 6 mice. Two-way ANOVA with post-hoc Tukey tests. k . Representative images of wisteria floribunda agglutinin (WFA) and parvalbumin (PV) staining in critical period VC. Scale bar, 20 µm. l . WFA integrated density around PV+ cells. 5 mice per group. Tdt no MD, n = 204 cells. tdT MD, n = 197 cells. CCN1 no MD, n = 228 cells. CCN1 MD, n = 193 cells. Kruskal Wallis test with post-hoc Dunn’s tests. m. Representative image of IBA1and CD68 staining in critical period VC. Scale bar, 10 µm . n. Volume of CD68+ puncta within IBA1 volume. Kruskal Wallis test with post-hoc Dunn’s tests. o . Representative image of IBA1 staining from critical period VC, top. Representative cell body and process tracing (skeleton), bottom. Scale bar, 10 µm. tdT no MD, 747 microglia from 4 mice. tdT MD, 876 microglia from 4 mice. CCN1 no MD, 496 microglia from 3 mice. CCN1 MD, 379 microglia from 3 mice. p . Proportion of microglial morphotypes-amoeboid, rod-like, ramified, and hyper-ramified. Black symbols, mouse averages. Two-way ANOVA with post-hoc Tukey tests. Only significant tests are shown. Bar charts show mean ± SEM. Box charts show median, upper and lower quartiles and whiskers show full range of data.

    Techniques Used: Plasmid Preparation, Patch Clamp, Staining

    biotinylated wisteria floribunda agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin
    Biotinylated Wisteria Floribunda Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin wfa  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa
    Biotinylated Wisteria Floribunda Agglutinin Wfa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin lectin
    Biotinylated Wisteria Floribunda Agglutinin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin wfa primary antibody  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa primary antibody
    Biotinylated Wisteria Floribunda Agglutinin Wfa Primary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin wfa  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa
    Biotinylated Wisteria Floribunda Agglutinin Wfa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin
    Biotinylated Wisteria Floribunda Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated wisteria floribunda agglutinin wfa  (Vector Laboratories)


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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa
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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa
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    Vector Laboratories biotinylated wisteria floribunda agglutinin

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    Biotinylated Wisteria Floribunda Agglutinin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated wisteria floribunda agglutinin wfa primary antibody

    Biotinylated Wisteria Floribunda Agglutinin Wfa Primary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Journal: iScience

    Article Title: Restoring vision in adult amblyopia by enhancing plasticity through deletion of the transcriptional repressor REST

    doi: 10.1016/j.isci.2024.109507

    Figure Lengend Snippet:

    Article Snippet: Slices were placed free-floating in multi-well plates, washed 3 times for 3 min in PBS for lectins (PBlec: 0.1 mM CaCl 2 , MnCl 2 , MgCl 2 in PBS) and transferred to blocking solution (10% BSA + 1% Triton X-100 in PBlec) for 1 h. They were then moved to a primary antibody/lectin bath comprised of biotinylated Wisteria floribunda agglutinin (WFA, 1:300, Vector Labs B1355), anti-PV guinea pig antibody (1:300, Synaptic Systems 195-004) and anti-Otx2 rabbit antibody (1:100, Proteintech 134971-AP) in PBlec overnight at 4 °C.

    Techniques: Plasmid Preparation, Virus, Software