biotinylated universal secondary antibody  (Vector Laboratories)

Journal: Nutrients

Article Title: Anti-Diabetic Obesity Effects of Wasabia Japonica Matsum Leaf Extract on 45% Kcal High-Fat Diet-Fed Mice

doi: 10.3390/nu12092837

Figure Lengend Snippet: Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

Article Snippet: This was followed by incubation with ABC reagents (Vectastain Elite ABC Kit, Vector Lab., Burlingame, CA, USA) and biotinylated universal secondary antibody (Vector Lab., Burlingame, CA, USA) in a humidity chamber for 1 h at room temperature.

Techniques: Mouse Assay, Avidin-Biotin Assay

Journal: Nutrients

Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

doi: 10.3390/nu6093536

Figure Lengend Snippet: Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

Article Snippet: Next day, the sections were incubated with biotinylated universal secondary antibody (Vector Lab., dilution 1:50) for 1 h at room temperature, and then avidin-biotin-peroxidase reagents (Vectastain Elite ABC Kit, Vector Lab., dilution 1:50) according to ABC methods [ ].

Techniques: Mouse Assay, Avidin-Biotin Assay

Journal: Nutrients

Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

doi: 10.3390/nu6093536

Figure Lengend Snippet: Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

Article Snippet: Next day, the sections were incubated with biotinylated universal secondary antibody (Vector Lab., dilution 1:50) for 1 h at room temperature, and then avidin-biotin-peroxidase reagents (Vectastain Elite ABC Kit, Vector Lab., dilution 1:50) according to ABC methods [ ].

Techniques: Mouse Assay, Avidin-Biotin Assay

Journal: Behavioural brain research

Article Title: HIPPOCAMPAL AND SUBICULAR EFFERENTS AND AFFERENTS OF THE PERIRHINAL, POSTRHINAL, AND ENTORHINAL CORTICES OF THE RAT

doi: 10.1016/j.bbr.2013.07.005

Figure Lengend Snippet: Examples of anterograde and retrograde tracer injection sites. Shown are a Pha-L site in POR, 40P at −8.1 mm relative to β (A), a biotinylated dextrane site in the LEA, 61B at −6.5 mm relative to β (B), a diamidino yellow

Article Snippet: Following two 10 minute washes in 2% NGS in KPBS, sections were incubated in the biotinylated secondary antibody solution of goat anti-rabbit IgG (1:277 dilution; Vector Laboratories, Burlingame, CA), 0.3% TX, and 2% NGS in KPBS for 1 hr.

Techniques: Injection

Journal: Arthritis Research & Therapy

Article Title: Cartilage-specific deletion of ephrin-B2 in mice results in early developmental defects and an osteoarthritis-like phenotype during aging in vivo

doi: 10.1186/s13075-016-0965-6

Figure Lengend Snippet: Locomotor phenotype of some ephrin-B2 ( EFNB2 ) Col2 knockout ( KO ) cartilage conditional mice is related to an abnormal corticospinal tract. a - f Brain tracing experiments to visualize the corticospinal path with biotinylated dextran amines ( BDA ) performed in 6-week-old EFNB2 fl/fl (n = 2) and EFNB2 Col2 KO cartilage conditional (n = 3) mice. Representative photomicrographs of the cervical spinal cord of EFNB2 fl/fl ( a , c , d ) and EFNB2 Col2 KO cartilage conditional mice ( b , e , f ) at the level of the fifth cervical vertebra. g Percentage of BDA-labeled fibers in the contralateral and ipsilateral hemispheres. Arrowheads indicate the injection site ( a , b ) and labeled fibers ( c - f ). Scale bars at 100 μm ( a , d ). Data are expressed as the mean ± standard error of the mean and the P values were determined by Mann–Whitney U test

Article Snippet: Each slide was washed three times in PBS (pH 7.4) and incubated with a secondary biotinylated antibody (anti-mouse, or anti-rabbit when appropriate) (Vector Laboratories Inc., Burlingame, CA, USA), then processed using the Vectastain ABC kit (Vector Laboratories) following the manufacturer’s instructions.

Techniques: Knock-Out, Mouse Assay, Labeling, Injection, MANN-WHITNEY