biotinylated universal secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Universal Antibody Horse Anti Mouse Rabbit IgG
    Description:
    Biotinylated Universal Antibody Horse Anti Mouse Rabbit IgG is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Universal Horse Anti Mouse Rabbit IgG H L is supplied in liquid format With some exceptions the recommended dilution for most applications is 1 50 H L indicates the antibody recognizes both heavy and light chains This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG H L and rabbit IgG H L primary antibodies The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti mouse IgG H L or biotinylated anti rabbit IgG H L used individually The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product Note This antibody has significant crossreactivity for rodent IgG and is not recommended for use in staining rodent tissue
    Catalog Number:
    ba-1400
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Universal Mouse Rabbit
    Size:
    2 1 mg
    Host:
    Horse
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated universal secondary antibody
    Biotinylated Universal Antibody Horse Anti Mouse Rabbit IgG
    Biotinylated Universal Antibody Horse Anti Mouse Rabbit IgG is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Universal Horse Anti Mouse Rabbit IgG H L is supplied in liquid format With some exceptions the recommended dilution for most applications is 1 50 H L indicates the antibody recognizes both heavy and light chains This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG H L and rabbit IgG H L primary antibodies The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti mouse IgG H L or biotinylated anti rabbit IgG H L used individually The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product Note This antibody has significant crossreactivity for rodent IgG and is not recommended for use in staining rodent tissue
    https://www.bioz.com/result/biotinylated universal secondary antibody/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated universal secondary antibody - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Fermentation of Green Tea with 2% Aquilariae lignum Increases the Anti-Diabetic Activity of Green Tea Aqueous Extracts in the High Fat-Fed Mouse"

    Article Title: Fermentation of Green Tea with 2% Aquilariae lignum Increases the Anti-Diabetic Activity of Green Tea Aqueous Extracts in the High Fat-Fed Mouse

    Journal: Nutrients

    doi: 10.3390/nu7115447

    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( A ) Intact control: Normal pellet diet supplied vehicle control mice; 10 mL/kg of distilled water oral administered mice; ( B ) HFD (vehicle) control: 10 mL/kg of distilled water oral administered mice with HFD supply; ( C ) Simvastatin: 10 mg/kg of simvastatin oral administered mice with HFD supply; ( D ) Metformin: 250 mg/kg of metformin oral administered mice with HFD supply; ( E ) GT400: 400 mg/kg of GT oral administered mice with HFD supply; ( F ) fGT400: 400 mg/kg of fGT oral administered mice with HFD supply; ( G ) fGT200: 200 mg/kg of fGT oral administered mice with HFD supply; ( H ) fGT100: 100 mg/kg of fGT oral administered mice with HFD supply. NFD, normal fat pellet diet; HFD, high fat diet; GT, green tea extracts; fGT, Aquilariae lignum -fermented green tea extracts. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.
    Figure Legend Snippet: Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( A ) Intact control: Normal pellet diet supplied vehicle control mice; 10 mL/kg of distilled water oral administered mice; ( B ) HFD (vehicle) control: 10 mL/kg of distilled water oral administered mice with HFD supply; ( C ) Simvastatin: 10 mg/kg of simvastatin oral administered mice with HFD supply; ( D ) Metformin: 250 mg/kg of metformin oral administered mice with HFD supply; ( E ) GT400: 400 mg/kg of GT oral administered mice with HFD supply; ( F ) fGT400: 400 mg/kg of fGT oral administered mice with HFD supply; ( G ) fGT200: 200 mg/kg of fGT oral administered mice with HFD supply; ( H ) fGT100: 100 mg/kg of fGT oral administered mice with HFD supply. NFD, normal fat pellet diet; HFD, high fat diet; GT, green tea extracts; fGT, Aquilariae lignum -fermented green tea extracts. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Techniques Used: Mouse Assay, Avidin-Biotin Assay

    2) Product Images from "Beneficial effects of dried pomegranate juice concentrated powder on ultraviolet B-induced skin photoaging in hairless mice"

    Article Title: Beneficial effects of dried pomegranate juice concentrated powder on ultraviolet B-induced skin photoaging in hairless mice

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2017.4626

    Representative immunohistochemistrical images of dorsal back skin tissues, taken from unexposed intact or UVB-exposed hairless mice. (A) Intact vehicle control; (B) UVB control; (C) UVB-exposed mice treated with 2 ml/kg PCS mice; (D) UVB-exposed mice treated with 100 mg/kg PCP; (E) UVB-exposed mice treated with 200 mg/kg PCP; (F) UVB-exposed mice treated with 400 mg/kg PCP. All avidin-biotin complex immunostaining. Scale bars=50 µm. UVB, ultraviolet B; 4-HNE, 4-hydroxynonenal; PARP, cleaved poly(ADP-ribose) polymerase; MMP-9, matrix metalloproteinase 9; PCP, dried pomegranate juice concentrated powder; PCS, pomegranate juice concentrated solution.
    Figure Legend Snippet: Representative immunohistochemistrical images of dorsal back skin tissues, taken from unexposed intact or UVB-exposed hairless mice. (A) Intact vehicle control; (B) UVB control; (C) UVB-exposed mice treated with 2 ml/kg PCS mice; (D) UVB-exposed mice treated with 100 mg/kg PCP; (E) UVB-exposed mice treated with 200 mg/kg PCP; (F) UVB-exposed mice treated with 400 mg/kg PCP. All avidin-biotin complex immunostaining. Scale bars=50 µm. UVB, ultraviolet B; 4-HNE, 4-hydroxynonenal; PARP, cleaved poly(ADP-ribose) polymerase; MMP-9, matrix metalloproteinase 9; PCP, dried pomegranate juice concentrated powder; PCS, pomegranate juice concentrated solution.

    Techniques Used: Mouse Assay, Avidin-Biotin Assay, Immunostaining

    3) Product Images from "Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice"

    Article Title: Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2015/179185

    Representative immunohistochemical images of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the submandibular LN tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Noticeable increases of the numbers of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells were observed in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these submandibular LN hypersensitivities related increases of cytokine immunoreactive cells were significantly inhibited by topical treatment of 1% DEXA, bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); LN = lymph node; IFN = interferon; IL = interleukin; iNOS = inducible nitric oxide synthase (2); TNF = tumor necrosis factor; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.
    Figure Legend Snippet: Representative immunohistochemical images of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the submandibular LN tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Noticeable increases of the numbers of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells were observed in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these submandibular LN hypersensitivities related increases of cytokine immunoreactive cells were significantly inhibited by topical treatment of 1% DEXA, bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); LN = lymph node; IFN = interferon; IL = interleukin; iNOS = inducible nitric oxide synthase (2); TNF = tumor necrosis factor; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.

    Techniques Used: Immunohistochemistry, Mouse Assay, Immunolabeling, Avidin-Biotin Assay

    Representative immunohistochemical images of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the splenic tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Dramatic and noticeable increases of the numbers of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells were observed in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these hypersensitivities related increases of cytokine immunoreactive cells were significantly inhibited by topical treatment of 1% DEXA and bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); IFN = interferon; IL = interleukin; iNOS = inducible nitric oxide synthase (2); TNF = tumor necrosis factor; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.
    Figure Legend Snippet: Representative immunohistochemical images of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the splenic tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Dramatic and noticeable increases of the numbers of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells were observed in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these hypersensitivities related increases of cytokine immunoreactive cells were significantly inhibited by topical treatment of 1% DEXA and bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); IFN = interferon; IL = interleukin; iNOS = inducible nitric oxide synthase (2); TNF = tumor necrosis factor; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.

    Techniques Used: Immunohistochemistry, Mouse Assay, Immunolabeling, Avidin-Biotin Assay

    Representative immunohistochemical images of dermal IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the dorsal back skin tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Dramatic infiltrations of dermal IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells were observed in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these increases of immunoreactivities of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the dermis were significantly inhibited by bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. Topical application of 1% DEXA also significantly reduced the increases of dermal IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells induced by NDCB treatment as compared with DNCB control mice, in this experiment. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); IFN = interferon; IL = interleukin; iNOS = inducible nitric oxide synthase (2); TNF = tumor necrosis factor; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.
    Figure Legend Snippet: Representative immunohistochemical images of dermal IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the dorsal back skin tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Dramatic infiltrations of dermal IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells were observed in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these increases of immunoreactivities of IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α in the dermis were significantly inhibited by bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. Topical application of 1% DEXA also significantly reduced the increases of dermal IFN- γ , iNOS, IL-1 β , IL-2, and TNF- α immunolabeled cells induced by NDCB treatment as compared with DNCB control mice, in this experiment. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); IFN = interferon; IL = interleukin; iNOS = inducible nitric oxide synthase (2); TNF = tumor necrosis factor; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.

    Techniques Used: Immunohistochemistry, Mouse Assay, Immunolabeling, Avidin-Biotin Assay

    Representative immunohistochemical images of caspase-3, PARP, NT, 4-HNE, and MMP-9 in the dorsal back skin tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Noticeable elevations of caspase-3, PARP, NT, and 4-HNE immunoreactive epidermal cells, dermal MMP-9 immunoreactivities were detected on the dorsal back skin tissues in DNCB control mice, respectively. However, these increases of immunoreactivities related AD signs were significantly inhibited by bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. Topical application of 1% DEXA also significantly reduced the increases of caspase-3, PARP, NT, and 4-HNE immunoreactive epidermal cells and dermal MMP-9 immunoreactivities as compared with DNCB control mice, in this experiment. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); PARP = cleaved poly (ADP-ribose) polymerase; NT = nitrotyrosine; 4-HNE = 4-hydroxynonenal; MMP = matrix metalloprotease; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.
    Figure Legend Snippet: Representative immunohistochemical images of caspase-3, PARP, NT, 4-HNE, and MMP-9 in the dorsal back skin tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Noticeable elevations of caspase-3, PARP, NT, and 4-HNE immunoreactive epidermal cells, dermal MMP-9 immunoreactivities were detected on the dorsal back skin tissues in DNCB control mice, respectively. However, these increases of immunoreactivities related AD signs were significantly inhibited by bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. Topical application of 1% DEXA also significantly reduced the increases of caspase-3, PARP, NT, and 4-HNE immunoreactive epidermal cells and dermal MMP-9 immunoreactivities as compared with DNCB control mice, in this experiment. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); PARP = cleaved poly (ADP-ribose) polymerase; NT = nitrotyrosine; 4-HNE = 4-hydroxynonenal; MMP = matrix metalloprotease; ABC = avidin-biotin complex. All being ABC immunostain. Scale bars = 40 μ m.

    Techniques Used: Immunohistochemistry, Mouse Assay, Avidin-Biotin Assay

    4) Product Images from "Anti-Diabetic Obesity Effects of Wasabia Japonica Matsum Leaf Extract on 45% Kcal High-Fat Diet-Fed Mice"

    Article Title: Anti-Diabetic Obesity Effects of Wasabia Japonica Matsum Leaf Extract on 45% Kcal High-Fat Diet-Fed Mice

    Journal: Nutrients

    doi: 10.3390/nu12092837

    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.
    Figure Legend Snippet: Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Techniques Used: Mouse Assay, Avidin-Biotin Assay

    5) Product Images from "Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse"

    Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

    Journal: Nutrients

    doi: 10.3390/nu6093536

    Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.
    Figure Legend Snippet: Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Techniques Used: Mouse Assay, Avidin-Biotin Assay

    Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.
    Figure Legend Snippet: Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Techniques Used: Mouse Assay, Avidin-Biotin Assay

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    Avidin-Biotin Assay:

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    Article Snippet: To visualize αV β6 in tissue sections purified 3G9 was biotinylated with N-hydroxysuccinimide biotin ester overnight at 4 °C, followed by a single desalting step with PD-10 columns in PBS with 0.1% BSA and 0.02% azide as preservative. .. Sections were blocked in 5% swine serum (Sigma) and 2% BSA (Sigma), incubated with biotinylated 3G9 antibody and then with Fluorescein Avidin D (Vector Labs). .. Slides were mounted with Vectamount containing DAPI (Vector Labs), and the staining was analyzed using a Nikon Eclipse 80i microscope (Nikon Instruments, Japan).

    Article Title: Surface Plasmon Resonance (SPR) Detection Using Antibody-Linked Magnetic Nanoparticles for Analyte Capture, Purification, Concentration and Signal Amplification
    Article Snippet: A 50 μg/mL avidin solution (Avidin D, Vector Labs #A2000) was then flowed through the SPR system. .. The unbiotinylated monoclonal anti-SEB antibodies served as a control and exhibited no avidin binding, while the commercially biotinylated antibodies from Vector Labs and the biotinylated monoclonal anti-SEB each bound similar amounts of avidin. .. Biotinylated antibodies were then mixed with 50 nanometer streptavidin-coated nanobeads (Miltenyi μMACS, Gladbach, Germany #120-001-017) by incubating 100 #l of the bead solution with an excess (50 μg) of biotinylated antibodies.

    Staining:

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1
    Article Snippet: .. Serial sections were stained with antibodies to CD31, CD102, CD144, CD106, Flk-1, and CD62E at 1 µg/ml, detected with biotinylated antirat IgG, and developed with the NovaRED substrate kit (Vector Laboratories). ..

    Binding Assay:

    Article Title: Surface Plasmon Resonance (SPR) Detection Using Antibody-Linked Magnetic Nanoparticles for Analyte Capture, Purification, Concentration and Signal Amplification
    Article Snippet: A 50 μg/mL avidin solution (Avidin D, Vector Labs #A2000) was then flowed through the SPR system. .. The unbiotinylated monoclonal anti-SEB antibodies served as a control and exhibited no avidin binding, while the commercially biotinylated antibodies from Vector Labs and the biotinylated monoclonal anti-SEB each bound similar amounts of avidin. .. Biotinylated antibodies were then mixed with 50 nanometer streptavidin-coated nanobeads (Miltenyi μMACS, Gladbach, Germany #120-001-017) by incubating 100 #l of the bead solution with an excess (50 μg) of biotinylated antibodies.

    Blocking Assay:

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation
    Article Snippet: In the IHC staining assay, the sections were deparaffinized, rehydrated, and then heated in the Tris-EDTA Buffer (pH 9.0) at 100 °C for 20 min. .. Subsequently, the sections were blocked with the block solution (1–3% protein) for 2 h, incubated with primary antibodies (anti-SARS-CoV SUD and anti-mouse CD11b (ab133357; Abcam)) at 4 °C overnight, treated in 3% H2 O2 for 10 min to quench the endogenous peroxidase activity, and reacted with biotinylated universal antibodies from a VECTASTAIN® Elite® ABC Universal Kit (Vector Laboratories, Burlingame, CA, USA) for 30 min. After incubating with VECTASTAIN elite ABC reagent 30 min, the dark brown-black precipitate of the immune complexes in the sections was developed using DAB Peroxidase Substrate Kit (Vector Laboratories). ..

    Activity Assay:

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation
    Article Snippet: In the IHC staining assay, the sections were deparaffinized, rehydrated, and then heated in the Tris-EDTA Buffer (pH 9.0) at 100 °C for 20 min. .. Subsequently, the sections were blocked with the block solution (1–3% protein) for 2 h, incubated with primary antibodies (anti-SARS-CoV SUD and anti-mouse CD11b (ab133357; Abcam)) at 4 °C overnight, treated in 3% H2 O2 for 10 min to quench the endogenous peroxidase activity, and reacted with biotinylated universal antibodies from a VECTASTAIN® Elite® ABC Universal Kit (Vector Laboratories, Burlingame, CA, USA) for 30 min. After incubating with VECTASTAIN elite ABC reagent 30 min, the dark brown-black precipitate of the immune complexes in the sections was developed using DAB Peroxidase Substrate Kit (Vector Laboratories). ..

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    Vector Laboratories biotinylated universal secondary antibody
    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by <t>avidin-biotin-peroxidase</t> complex. Scale bars = 80 µm.
    Biotinylated Universal Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Anti-Diabetic Obesity Effects of Wasabia Japonica Matsum Leaf Extract on 45% Kcal High-Fat Diet-Fed Mice

    doi: 10.3390/nu12092837

    Figure Lengend Snippet: Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: This was followed by incubation with ABC reagents (Vectastain Elite ABC Kit, Vector Lab., Burlingame, CA, USA) and biotinylated universal secondary antibody (Vector Lab., Burlingame, CA, USA) in a humidity chamber for 1 h at room temperature.

    Techniques: Mouse Assay, Avidin-Biotin Assay

    Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

    doi: 10.3390/nu6093536

    Figure Lengend Snippet: Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: Next day, the sections were incubated with biotinylated universal secondary antibody (Vector Lab., dilution 1:50) for 1 h at room temperature, and then avidin-biotin-peroxidase reagents (Vectastain Elite ABC Kit, Vector Lab., dilution 1:50) according to ABC methods [ ].

    Techniques: Mouse Assay, Avidin-Biotin Assay

    Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

    doi: 10.3390/nu6093536

    Figure Lengend Snippet: Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: Next day, the sections were incubated with biotinylated universal secondary antibody (Vector Lab., dilution 1:50) for 1 h at room temperature, and then avidin-biotin-peroxidase reagents (Vectastain Elite ABC Kit, Vector Lab., dilution 1:50) according to ABC methods [ ].

    Techniques: Mouse Assay, Avidin-Biotin Assay

    Examples of anterograde and retrograde tracer injection sites. Shown are a Pha-L site in POR, 40P at −8.1 mm relative to β (A), a biotinylated dextrane site in the LEA, 61B at −6.5 mm relative to β (B), a diamidino yellow

    Journal: Behavioural brain research

    Article Title: HIPPOCAMPAL AND SUBICULAR EFFERENTS AND AFFERENTS OF THE PERIRHINAL, POSTRHINAL, AND ENTORHINAL CORTICES OF THE RAT

    doi: 10.1016/j.bbr.2013.07.005

    Figure Lengend Snippet: Examples of anterograde and retrograde tracer injection sites. Shown are a Pha-L site in POR, 40P at −8.1 mm relative to β (A), a biotinylated dextrane site in the LEA, 61B at −6.5 mm relative to β (B), a diamidino yellow

    Article Snippet: Following two 10 minute washes in 2% NGS in KPBS, sections were incubated in the biotinylated secondary antibody solution of goat anti-rabbit IgG (1:277 dilution; Vector Laboratories, Burlingame, CA), 0.3% TX, and 2% NGS in KPBS for 1 hr.

    Techniques: Injection

    Locomotor phenotype of some ephrin-B2 ( EFNB2 ) Col2 knockout ( KO ) cartilage conditional mice is related to an abnormal corticospinal tract. a - f Brain tracing experiments to visualize the corticospinal path with biotinylated dextran amines ( BDA ) performed in 6-week-old EFNB2 fl/fl (n = 2) and EFNB2 Col2 KO cartilage conditional (n = 3) mice. Representative photomicrographs of the cervical spinal cord of EFNB2 fl/fl ( a , c , d ) and EFNB2 Col2 KO cartilage conditional mice ( b , e , f ) at the level of the fifth cervical vertebra. g Percentage of BDA-labeled fibers in the contralateral and ipsilateral hemispheres. Arrowheads indicate the injection site ( a , b ) and labeled fibers ( c - f ). Scale bars at 100 μm ( a , d ). Data are expressed as the mean ± standard error of the mean and the P values were determined by Mann–Whitney U test

    Journal: Arthritis Research & Therapy

    Article Title: Cartilage-specific deletion of ephrin-B2 in mice results in early developmental defects and an osteoarthritis-like phenotype during aging in vivo

    doi: 10.1186/s13075-016-0965-6

    Figure Lengend Snippet: Locomotor phenotype of some ephrin-B2 ( EFNB2 ) Col2 knockout ( KO ) cartilage conditional mice is related to an abnormal corticospinal tract. a - f Brain tracing experiments to visualize the corticospinal path with biotinylated dextran amines ( BDA ) performed in 6-week-old EFNB2 fl/fl (n = 2) and EFNB2 Col2 KO cartilage conditional (n = 3) mice. Representative photomicrographs of the cervical spinal cord of EFNB2 fl/fl ( a , c , d ) and EFNB2 Col2 KO cartilage conditional mice ( b , e , f ) at the level of the fifth cervical vertebra. g Percentage of BDA-labeled fibers in the contralateral and ipsilateral hemispheres. Arrowheads indicate the injection site ( a , b ) and labeled fibers ( c - f ). Scale bars at 100 μm ( a , d ). Data are expressed as the mean ± standard error of the mean and the P values were determined by Mann–Whitney U test

    Article Snippet: Each slide was washed three times in PBS (pH 7.4) and incubated with a secondary biotinylated antibody (anti-mouse, or anti-rabbit when appropriate) (Vector Laboratories Inc., Burlingame, CA, USA), then processed using the Vectastain ABC kit (Vector Laboratories) following the manufacturer’s instructions.

    Techniques: Knock-Out, Mouse Assay, Labeling, Injection, MANN-WHITNEY