biotinylated soybean agglutinin sba  (Vector Laboratories)


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    Vector Laboratories biotinylated soybean agglutinin sba
    Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with <t>biotinylated</t> soybean <t>agglutinin</t> detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .
    Biotinylated Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Evaluation of a FlpA Glycoconjugate Vaccine with Ten N -Heptasaccharide Glycan Moieties to reduce Campylobacter jejuni Colonisation in Chickens"

    Article Title: Evaluation of a FlpA Glycoconjugate Vaccine with Ten N -Heptasaccharide Glycan Moieties to reduce Campylobacter jejuni Colonisation in Chickens

    Journal: Vaccines

    doi: 10.3390/vaccines12040395

    Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with biotinylated soybean agglutinin detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .
    Figure Legend Snippet: Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with biotinylated soybean agglutinin detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .

    Techniques Used: SDS Page, Affinity Purification, Molecular Weight, Staining

    biotinylated soybean agglutinin sba  (Vector Laboratories)


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    Vector Laboratories biotinylated soybean agglutinin sba
    Biotinylated Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated soybean agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated soybean agglutinin
    Biotinylated Soybean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated soybean agglutinin/product/Vector Laboratories
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    biotinylated soybean agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated soybean agglutinin
    Biotinylated Soybean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated soybean agglutinin/product/Vector Laboratories
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    biotinylated soybean agglutinin sba  (Vector Laboratories)


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    Vector Laboratories biotinylated soybean agglutinin sba
    Biotinylated Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b 1015  (Vector Laboratories)


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    Vector Laboratories b 1015
    B 1015, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated lectin
    List of antibodies used for staining.
    Biotinylated Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts"

    Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007475

    List of antibodies used for staining.
    Figure Legend Snippet: List of antibodies used for staining.

    Techniques Used: Staining, Plasmid Preparation

    biotinylated sba lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated sba lectin
    Biotinylated Sba Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lectin solution  (Vector Laboratories)


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    Vector Laboratories lectin solution
    Lectin Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated soybean agglutinin sba  (Vector Laboratories)


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    Vector Laboratories biotinylated soybean agglutinin sba
    a Schematic illustration of a structure-switchable DNA-based glycan chip platform using a pH-responsive i-motif DNA linker. b Schematic illustration and scanned raw image for hybridization of complementary single-stranded oligonucleotides with surface-immobilized i-motif DNAs under basic conditions (pH 9.0). c – g Schematic illustrations and scanned raw images for hybridization and denaturation of lactose-oligonucleotide conjugates by pH-responsive structural change in surface-immobilized i-motif DNAs. c The first round hybridization of lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions (pH 9.0). d The first round of denaturation of lactose-oligonucleotide conjugates from surface-immobilized i-motif DNAs under acidic conditions (pH 4.5). e The second round of hybridization of isolated lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions. f The second round of denaturation of lactose-oligonucleotide conjugates from surface-immobilized i-motif DNAs under acidic conditions. g The third round of hybridization of isolated lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions. The hybridized lactose-oligonucleotide conjugates were detected by using <t>biotinylated</t> RCA 120 lectin and Alexa Fluor ® 647-conjugated streptavidin. Scale bar is 800 μm. Symbols: blue circle, Glc; yellow circle, Gal.
    Biotinylated Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Glycan chip based on structure-switchable DNA linker for on-chip biosynthesis of cancer-associated complex glycans"

    Article Title: Glycan chip based on structure-switchable DNA linker for on-chip biosynthesis of cancer-associated complex glycans

    Journal: Nature Communications

    doi: 10.1038/s41467-021-21538-0

    a Schematic illustration of a structure-switchable DNA-based glycan chip platform using a pH-responsive i-motif DNA linker. b Schematic illustration and scanned raw image for hybridization of complementary single-stranded oligonucleotides with surface-immobilized i-motif DNAs under basic conditions (pH 9.0). c – g Schematic illustrations and scanned raw images for hybridization and denaturation of lactose-oligonucleotide conjugates by pH-responsive structural change in surface-immobilized i-motif DNAs. c The first round hybridization of lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions (pH 9.0). d The first round of denaturation of lactose-oligonucleotide conjugates from surface-immobilized i-motif DNAs under acidic conditions (pH 4.5). e The second round of hybridization of isolated lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions. f The second round of denaturation of lactose-oligonucleotide conjugates from surface-immobilized i-motif DNAs under acidic conditions. g The third round of hybridization of isolated lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions. The hybridized lactose-oligonucleotide conjugates were detected by using biotinylated RCA 120 lectin and Alexa Fluor ® 647-conjugated streptavidin. Scale bar is 800 μm. Symbols: blue circle, Glc; yellow circle, Gal.
    Figure Legend Snippet: a Schematic illustration of a structure-switchable DNA-based glycan chip platform using a pH-responsive i-motif DNA linker. b Schematic illustration and scanned raw image for hybridization of complementary single-stranded oligonucleotides with surface-immobilized i-motif DNAs under basic conditions (pH 9.0). c – g Schematic illustrations and scanned raw images for hybridization and denaturation of lactose-oligonucleotide conjugates by pH-responsive structural change in surface-immobilized i-motif DNAs. c The first round hybridization of lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions (pH 9.0). d The first round of denaturation of lactose-oligonucleotide conjugates from surface-immobilized i-motif DNAs under acidic conditions (pH 4.5). e The second round of hybridization of isolated lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions. f The second round of denaturation of lactose-oligonucleotide conjugates from surface-immobilized i-motif DNAs under acidic conditions. g The third round of hybridization of isolated lactose-oligonucleotide conjugates with surface-immobilized i-motif DNAs under basic conditions. The hybridized lactose-oligonucleotide conjugates were detected by using biotinylated RCA 120 lectin and Alexa Fluor ® 647-conjugated streptavidin. Scale bar is 800 μm. Symbols: blue circle, Glc; yellow circle, Gal.

    Techniques Used: Hybridization, Isolation

    Scanned raw images and quantitative intensity plots for on-chip biosynthesized ( a ) Gb3 trisaccharide, ( b ) Gb4 tetrasaccharide, ( c ) Gb5 pentasaccharide, ( d ) SSEA-4 hexasaccharide, and ( e ) Globo H hexasaccharide. Synthesized complex glycans were detected by using biotinylated lectins and Alexa Fluor ® 647-conjugated streptavidin. Each value presents the mean ± SEM from forty-nine independent spots excluding the highest and lowest signals. Statistical significance was assessed using Student’s unpaired t test (**** p < 0.0001). GS-IB 4 Griffonia simplicifolia isolectin B 4 , SBA soybean agglutinin lectin, RCA 120 Ricinus communis agglutinin I lectin, MAL II Maackia amurensis lectin II, LTL Lotus tetragonolobus lectin, LgtC α-1,4-galactosyltransferase, LgtD β-1,3- N -acetylgalactosaminyltransferase/β-1,3-galactosyltransferase, α2,3-SialT α-2,3-sialyltransferase, α1,2-FucT α-1,2-fucosyltransferase. Symbols: blue circle, Glc; yellow circle, Gal; yellow square, GalNAc; red triangle, Fuc; purple square, Neu5Ac. Scale bar is 800 μm. Source data are provided as a .
    Figure Legend Snippet: Scanned raw images and quantitative intensity plots for on-chip biosynthesized ( a ) Gb3 trisaccharide, ( b ) Gb4 tetrasaccharide, ( c ) Gb5 pentasaccharide, ( d ) SSEA-4 hexasaccharide, and ( e ) Globo H hexasaccharide. Synthesized complex glycans were detected by using biotinylated lectins and Alexa Fluor ® 647-conjugated streptavidin. Each value presents the mean ± SEM from forty-nine independent spots excluding the highest and lowest signals. Statistical significance was assessed using Student’s unpaired t test (**** p < 0.0001). GS-IB 4 Griffonia simplicifolia isolectin B 4 , SBA soybean agglutinin lectin, RCA 120 Ricinus communis agglutinin I lectin, MAL II Maackia amurensis lectin II, LTL Lotus tetragonolobus lectin, LgtC α-1,4-galactosyltransferase, LgtD β-1,3- N -acetylgalactosaminyltransferase/β-1,3-galactosyltransferase, α2,3-SialT α-2,3-sialyltransferase, α1,2-FucT α-1,2-fucosyltransferase. Symbols: blue circle, Glc; yellow circle, Gal; yellow square, GalNAc; red triangle, Fuc; purple square, Neu5Ac. Scale bar is 800 μm. Source data are provided as a .

    Techniques Used: Synthesized

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    Vector Laboratories biotinylated soybean agglutinin sba
    Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with <t>biotinylated</t> soybean <t>agglutinin</t> detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .
    Biotinylated Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated soybean agglutinin
    Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with <t>biotinylated</t> soybean <t>agglutinin</t> detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .
    Biotinylated Soybean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated soybean agglutinin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
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    biotinylated soybean agglutinin - by Bioz Stars, 2024-06
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    93
    Vector Laboratories b 1015
    Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with <t>biotinylated</t> soybean <t>agglutinin</t> detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .
    B 1015, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated lectin
    List of antibodies used for staining.
    Biotinylated Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated sba lectin
    List of antibodies used for staining.
    Biotinylated Sba Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories lectin solution
    List of antibodies used for staining.
    Lectin Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with biotinylated soybean agglutinin detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .

    Journal: Vaccines

    Article Title: Evaluation of a FlpA Glycoconjugate Vaccine with Ten N -Heptasaccharide Glycan Moieties to reduce Campylobacter jejuni Colonisation in Chickens

    doi: 10.3390/vaccines12040395

    Figure Lengend Snippet: Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with biotinylated soybean agglutinin detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .

    Article Snippet: The purity of FlpA and FlpA-10GT was assessed by Coomassie staining of proteins resolved by SDS-PAGE and Western blotting using a mouse monoclonal anti-6×His 680LT conjugate (Li-COR, Cambridge, UK) at 1:10,000 to detect the protein and biotinylated soybean agglutinin (SBA) at 1:400 (Vector Laboratories, Newark, CA, USA) together with streptavidin IRDye 800CW at 1:4000 (Li-COR, Cambridge, UK) to detect glycosylated protein.

    Techniques: SDS Page, Affinity Purification, Molecular Weight, Staining

    List of antibodies used for staining.

    Journal: PLoS ONE

    Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

    doi: 10.1371/journal.pone.0007475

    Figure Lengend Snippet: List of antibodies used for staining.

    Article Snippet: Soybean agglutinin (SBA) , B-1015 , Biotinylated lectin , Vector Laboratories.

    Techniques: Staining, Plasmid Preparation