biotinylated soybean agglutinin sba  (Vector Laboratories)

Journal: Vaccines

Article Title: Evaluation of a FlpA Glycoconjugate Vaccine with Ten N -Heptasaccharide Glycan Moieties to reduce Campylobacter jejuni Colonisation in Chickens

doi: 10.3390/vaccines12040395

Figure Lengend Snippet: Purity and glycosylation of vaccine antigens. Panel ( A ) shows SDS-PAGE analysis of affinity purified FlpA, FlpA-2×GT, and FlpA-10×GT with proteins of the expected size. For FlpA-2×GT and FlpA-10×GT, species of a higher molecular weight correspond to N -glycosylated variants, as evident from staining with biotinylated soybean agglutinin detected with a streptavidin fluorophore conjugate as shown in panel ( B ) (green). The proteins were detected in panel ( B ) using mouse monoclonal anti-6xHis conjugated to the 680LT fluorophore (red). Original, uncropped images can be found in the .

Article Snippet: The purity of FlpA and FlpA-10GT was assessed by Coomassie staining of proteins resolved by SDS-PAGE and Western blotting using a mouse monoclonal anti-6×His 680LT conjugate (Li-COR, Cambridge, UK) at 1:10,000 to detect the protein and biotinylated soybean agglutinin (SBA) at 1:400 (Vector Laboratories, Newark, CA, USA) together with streptavidin IRDye 800CW at 1:4000 (Li-COR, Cambridge, UK) to detect glycosylated protein.

Techniques: SDS Page, Affinity Purification, Molecular Weight, Staining

Journal: iScience

Article Title: Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissues

doi: 10.1016/j.isci.2023.108219

Figure Lengend Snippet: Lectins and their glycan-binding specificities

Article Snippet: Aleuria Aurantia Lectin (AAL), Biotinylated , Vector Laboratories, UK , Cat# B-1015-5.

Techniques: Binding Assay

Journal: iScience

Article Title: Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissues

doi: 10.1016/j.isci.2023.108219

Figure Lengend Snippet: Human cancer CDX and normal tissue lysates were subjected to lectin affinity capture (A) Captured proteins were assessed by immunoblotting for CD13 using mAb 400–500 anti-CD13 antibody. Loading was assessed by Coomassie stain ( Figure S4 ). (B and C) Effect of sialo-glycan lectin binding on CD13 detection using mAb 400–500 anti-CD13 antibody and in cancer CDX and normal tissues. (D) Significance of glycosylation on the metabolic half live (min) of CD13 substrate in MCF-7 CDX homogenate. Data shown are the mean of 3 independent experiments ±SEM. ∗∗∗p > 0.001 and ∗∗∗∗p > 0.0001 (two-way ANOVA).

Article Snippet: Aleuria Aurantia Lectin (AAL), Biotinylated , Vector Laboratories, UK , Cat# B-1015-5.

Techniques: Western Blot, Staining, Binding Assay

Journal: iScience

Article Title: Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissues

doi: 10.1016/j.isci.2023.108219

Figure Lengend Snippet: CD13 detection in lectin-affinity captured proteins from human cancer CDX and normal tissues

Article Snippet: Aleuria Aurantia Lectin (AAL), Biotinylated , Vector Laboratories, UK , Cat# B-1015-5.

Techniques:

Journal: iScience

Article Title: Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissues

doi: 10.1016/j.isci.2023.108219

Figure Lengend Snippet:

Article Snippet: Aleuria Aurantia Lectin (AAL), Biotinylated , Vector Laboratories, UK , Cat# B-1015-5.

Techniques: Recombinant, Saline, Reverse Transcription, SYBR Green Assay, Plasmid Preparation, Western Blot, Knock-Out

Journal: iScience

Article Title: Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissues

doi: 10.1016/j.isci.2023.108219

Figure Lengend Snippet: Lectins and their glycan-binding specificities

Article Snippet: Soybean Agglutinin (SBA), Biotinylated , Vector Laboratories, UK , Cat# B-1075-5.

Techniques: Binding Assay

Journal: iScience

Article Title: Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissues

doi: 10.1016/j.isci.2023.108219

Figure Lengend Snippet:

Article Snippet: Soybean Agglutinin (SBA), Biotinylated , Vector Laboratories, UK , Cat# B-1075-5.

Techniques: Recombinant, Saline, Reverse Transcription, SYBR Green Assay, Plasmid Preparation, Western Blot, Knock-Out